Rroxscaffold_4G00330420

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000709
Physical Location & Seq
Forward (+)
64115538 .. 64117543
2006 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00330420.1

Sequence Viewer

Length: 993 bp
ATGAGTGGAGAAAGGAAGGTTGTGTGTGTTACAGGAGCTTCTGGTTTCATAGCTTCATGGCTGGTGAAGCTCTTGTTACAGCGAGGTTATGTCGTCAAAGCCACCGTTCGTGACCCAAATAATCCGAAGAAAACAGAACACTTGCTCTCACTTGAGGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAGGGGTCTTTTGACCTTGTAGTTGATGGATGTGAAGGTGTTTTCCACACAGCATCTCCGGTACTATTTTCATCGATCAGCGACCCACAGGTTCATCGGATCTCCGCAGAAGTGATTGATCCTGCAGTAAAGGGAACGCTTAATGTTCTTAAATCGTGCGTGAAATTTCCAACAATCAAGAGGGTAGTTTTAACATCTTCCATGGCGGCAGTTATAGTCAATGGAAGACCTTTAACCTCTAATGTGGTAGTAGATGAAACATGGTTTTCTGATCCACTTGTTTGTCAGGAGTTGAAGCAATGGTATTTTCTTTCGAAAACTTTAGCAGAGGAGGCTGCTTGGAAATTTGCTAAAGAAAACGGGATTGACATGGTGACCATAAATCCCTCGTACGTGATGGGTCCGCCCTTGCAGCCAACTCTTAATTTGAGTGTGGAGATGGTTCTGGATCTCAAGAAGGGTGTCAACGGAGTAGCAATTGACAATTATACATCTACTGATGTTAGAGATGTTGCCTCTGCTCATATTCAAGCATTTGAAGTTCCTTCAGCTAGTGGAAGATATTGTTTAGTTGCAAATGTCACCCCTATTCACAAGGCTCTGAAGATTTTAAAGGAACTTCATCCTACTTTGTGCCCACCTGAAATATGTGAGCATGATATCCCTTCTGGCCCAGAGTATCAAATATCCCAGGAAAAAGCAAAAAGTTTGGGAGTTAGTTTCCTTCCATTGGAAGTAAGTCTCAGGGACACTGTTGAATGCCTGAAGGAGAAGGGCTTCCTCAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

330

Amino Acids

36.15

Weight (kDa)

6.26

Isoelectric Point (pI)

26.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 86 3.5e-07 NmrA-like family
Epimerase PF01370 8 - 251 1.4e-19 NAD dependent epimerase/dehydratase family
KR PF08659 8 - 141 6.4e-06 KR domain
3Beta_HSD PF01073 10 - 246 2.5e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 206 1.2e-11 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 135 1.7e-09 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 138 2.9e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 306, 407, 605
AclWI GGATC 4 cut(s) 308, 314, 467, 657
AcsI RAATTY 2 cut(s) 365, 545
AcuI CTGAAG 3 cut(s) 732, 824, 986
AfaI GTAC 2 cut(s) 264, 593
AfiI CCNNNNNNNGG 1 cut(s) 931
AgsI TTSAA 5 cut(s) 184, 496, 731, 740, 959
AjnI CCWGG 1 cut(s) 891
AluBI AGCT 4 cut(s) 38, 53, 70, 752
AluI AGCT 4 cut(s) 38, 53, 70, 752
Alw26I GTCTC 1 cut(s) 947
AlwI GGATC 4 cut(s) 308, 314, 467, 657
AoxI GGCC 1 cut(s) 871
ApeKI GCWGC 2 cut(s) 536, 613
ApoI RAATTY 2 cut(s) 365, 545
Asp700I GAANNNNTTC 2 cut(s) 171, 977
AspS9I GGNCC 2 cut(s) 602, 872
AsuHPI GGTGA 3 cut(s) 76, 586, 775
AsuII TTCGAA 1 cut(s) 515
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 1 cut(s) 839
BbsI GAAGAC 1 cut(s) 433
BbvI GCAGC 2 cut(s) 523, 625
BccI CCATC 3 cut(s) 221, 592, 634
BciT130I CCWGG 1 cut(s) 893
BcoDI GTCTC 1 cut(s) 947
BfaI CTAG 1 cut(s) 753
BfmI CTRYAG 1 cut(s) 324
BisI GCNGC 3 cut(s) 408, 537, 614
BlsI GCNGC 3 cut(s) 409, 538, 615
Bme1390I CCNGG 1 cut(s) 893
Bme18I GGWCC 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 602, 872
BmiI GGNNCC 1 cut(s) 603
BmrFI CCNGG 1 cut(s) 893
BmsI GCATC 1 cut(s) 263
BpiI GAAGAC 1 cut(s) 433
Bpu14I TTCGAA 1 cut(s) 515
BpuEI CTTGAG 3 cut(s) 173, 638, 968
Bsa29I ATCGAT 1 cut(s) 275
BsaAI YACGTR 1 cut(s) 595
BsaJI CCNNGG 2 cut(s) 402, 891
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 931
Bse3DI GCAATG 1 cut(s) 506
BseBI CCWGG 1 cut(s) 893
BseCI ATCGAT 1 cut(s) 275
BseDI CCNNGG 2 cut(s) 402, 891
BseGI GGATG 2 cut(s) 236, 823
BseLI CCNNNNNNNGG 1 cut(s) 931
BseMI GCAATG 1 cut(s) 506
BseMII CTCAG 1 cut(s) 958
BseRI GAGGAG 1 cut(s) 545
BseSI GKGCMC 1 cut(s) 839
BseXI GCAGC 2 cut(s) 523, 625
BshFI GGCC 1 cut(s) 873
BshVI ATCGAT 1 cut(s) 275
BsiSI CCGG 1 cut(s) 260
BsiWI CGTACG 1 cut(s) 591
BslFI GGGAC 1 cut(s) 962
BslI CCNNNNNNNGG 1 cut(s) 931
BsmAI GTCTC 1 cut(s) 947
BsmFI GGGAC 1 cut(s) 962
BsmI GAATGC 1 cut(s) 965
BsnI GGCC 1 cut(s) 873
Bsp119I TTCGAA 1 cut(s) 515
Bsp1286I GDGCHC 1 cut(s) 839
Bsp143I GATC 5 cut(s) 276, 300, 319, 472, 649
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 3 cut(s) 306, 407, 605
BspANI GGCC 1 cut(s) 873
BspCNI CTCAG 1 cut(s) 957
BspDI ATCGAT 1 cut(s) 275
BspLI GGNNCC 1 cut(s) 603
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 4 cut(s) 308, 314, 467, 657
BspT104I TTCGAA 1 cut(s) 515
BsrDI GCAATG 1 cut(s) 506
BssECI CCNNGG 2 cut(s) 402, 891
BssMI GATC 5 cut(s) 276, 300, 319, 472, 649
BssT1I CCWWGG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 893
Bst4CI ACNGT 2 cut(s) 106, 955
Bst6I CTCTTC 1 cut(s) 195
BstBAI YACGTR 1 cut(s) 595
BstBI TTCGAA 1 cut(s) 515
BstDEI CTNAG 1 cut(s) 944
BstDSI CCRYGG 1 cut(s) 402
BstEII GGTNACC 1 cut(s) 574
BstF5I GGATG 2 cut(s) 236, 823
BstKTI GATC 5 cut(s) 279, 303, 322, 475, 652
BstMAI GTCTC 1 cut(s) 947
BstMBI GATC 5 cut(s) 276, 300, 319, 472, 649
BstMWI GCNNNNNNNGC 3 cut(s) 67, 533, 613
BstNI CCWGG 1 cut(s) 893
BstPI GGTNACC 1 cut(s) 574
BstSCI CCNGG 1 cut(s) 891
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 839
BstV1I GCAGC 2 cut(s) 523, 625
BstV2I GAAGAC 1 cut(s) 433
BstX2I RGATCY 2 cut(s) 300, 649
BstYI RGATCY 2 cut(s) 300, 649
Bsu15I ATCGAT 1 cut(s) 275
BsuRI GGCC 1 cut(s) 873
BsuTUI ATCGAT 1 cut(s) 275
BtgI CCRYGG 1 cut(s) 402
BtsCI GGATG 2 cut(s) 236, 823
BtsIMutI CAGTG 1 cut(s) 951
Cfr13I GGNCC 2 cut(s) 602, 872
ClaI ATCGAT 1 cut(s) 275
Csp6I GTAC 2 cut(s) 263, 592
CviAII CATG 5 cut(s) 57, 403, 462, 571, 857
CviQI GTAC 2 cut(s) 263, 592
DdeI CTNAG 1 cut(s) 944
DpnI GATC 5 cut(s) 278, 302, 321, 474, 651
DpnII GATC 5 cut(s) 276, 300, 319, 472, 649
DraI TTTAAA 1 cut(s) 813
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
EciI GGCGGA 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 402
Eco32I GATATC 1 cut(s) 862
Eco47I GGWCC 1 cut(s) 602
Eco57I CTGAAG 3 cut(s) 732, 824, 986
Eco91I GGTNACC 1 cut(s) 574
EcoO65I GGTNACC 1 cut(s) 574
EcoRII CCWGG 1 cut(s) 891
EcoRV GATATC 1 cut(s) 862
EcoT14I CCWWGG 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 5 cut(s) 60, 406, 465, 574, 860
FaqI GGGAC 1 cut(s) 962
FatI CATG 5 cut(s) 56, 402, 461, 570, 856
Fnu4HI GCNGC 3 cut(s) 408, 537, 614
FokI GGATG 2 cut(s) 243, 810
Fsp4HI GCNGC 3 cut(s) 408, 537, 614
FspBI CTAG 1 cut(s) 753
GluI GCNGC 3 cut(s) 408, 537, 614
HaeIII GGCC 1 cut(s) 873
HapII CCGG 1 cut(s) 260
Hin1II CATG 5 cut(s) 60, 406, 465, 574, 860
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HpaII CCGG 1 cut(s) 260
HphI GGTGA 3 cut(s) 76, 586, 775
Hpy166II GTNNAC 1 cut(s) 667
Hpy188I TCNGA 4 cut(s) 126, 300, 472, 804
Hpy188III TCNNGA 5 cut(s) 110, 379, 488, 647, 655
Hpy8I GTNNAC 1 cut(s) 667
HpyAV CCTTC 8 cut(s) 10, 230, 652, 756, 876, 935, 961, 967
HpyCH4III ACNGT 2 cut(s) 106, 955
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 3 cut(s) 326, 613, 776
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 533, 613
HpyF3I CTNAG 1 cut(s) 944
HpySE526I ACGT 1 cut(s) 594
Hsp92II CATG 5 cut(s) 60, 406, 465, 574, 860
Kzo9I GATC 5 cut(s) 276, 300, 319, 472, 649
LmnI GCTCC 2 cut(s) 35, 158
Lsp1109I GCAGC 2 cut(s) 523, 625
LweI GCATC 1 cut(s) 263
MaeI CTAG 1 cut(s) 753
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 5 cut(s) 28, 75, 110, 574, 781
MalI GATC 5 cut(s) 278, 302, 321, 474, 651
MboI GATC 5 cut(s) 276, 300, 319, 472, 649
MboII GAAGA 6 cut(s) 139, 212, 390, 438, 771, 817
MfeI CAATTG 1 cut(s) 678
MflI RGATCY 2 cut(s) 300, 649
MhlI GDGCHC 1 cut(s) 839
MluCI AATT 5 cut(s) 365, 545, 625, 678, 685
MmeI TCCRAC 1 cut(s) 395
MroXI GAANNNNTTC 2 cut(s) 171, 977
MseI TTAA 6 cut(s) 342, 351, 392, 434, 624, 812
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 893
MunI CAATTG 1 cut(s) 678
Mva1269I GAATGC 1 cut(s) 965
MvaI CCWGG 1 cut(s) 893
MwoI GCNNNNNNNGC 3 cut(s) 67, 533, 613
NcoI CCATGG 1 cut(s) 402
NdeII GATC 5 cut(s) 276, 300, 319, 472, 649
NlaIII CATG 5 cut(s) 60, 406, 465, 574, 860
NlaIV GGNNCC 1 cut(s) 603
NmuCI GTSAC 3 cut(s) 110, 574, 781
NspV TTCGAA 1 cut(s) 515
PctI GAATGC 1 cut(s) 965
PdmI GAANNNNTTC 2 cut(s) 171, 977
Pfl23II CGTACG 1 cut(s) 591
PkrI GCNGC 3 cut(s) 409, 538, 615
Ppu21I YACGTR 1 cut(s) 595
Psp6I CCWGG 1 cut(s) 891
PspEI GGTNACC 1 cut(s) 574
PspGI CCWGG 1 cut(s) 891
PspLI CGTACG 1 cut(s) 591
PspN4I GGNNCC 1 cut(s) 603
PspPI GGNCC 2 cut(s) 602, 872
PstI CTGCAG 1 cut(s) 328
PsuI RGATCY 2 cut(s) 300, 649
RsaI GTAC 2 cut(s) 264, 593
RsaNI GTAC 2 cut(s) 263, 592
SaqAI TTAA 6 cut(s) 342, 351, 392, 434, 624, 812
SatI GCNGC 3 cut(s) 408, 537, 614
Sau3AI GATC 5 cut(s) 276, 300, 319, 472, 649
Sau96I GGNCC 2 cut(s) 602, 872
ScrFI CCNGG 1 cut(s) 893
SduI GDGCHC 1 cut(s) 839
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 1 cut(s) 324
SfuI TTCGAA 1 cut(s) 515
SinI GGWCC 1 cut(s) 602
SmlI CTYRAG 3 cut(s) 152, 653, 983
SmoI CTYRAG 3 cut(s) 152, 653, 983
Sse9I AATT 5 cut(s) 365, 545, 625, 678, 685
SsiI CCGC 3 cut(s) 306, 407, 605
SspMI CTAG 1 cut(s) 753
StyD4I CCNGG 1 cut(s) 891
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 2 cut(s) 106, 955
TaiI ACGT 1 cut(s) 597
TaqI TCGA 2 cut(s) 275, 515
TasI AATT 5 cut(s) 365, 545, 625, 678, 685
TauI GCSGC 1 cut(s) 410
Tru1I TTAA 6 cut(s) 342, 351, 392, 434, 624, 812
Tru9I TTAA 6 cut(s) 342, 351, 392, 434, 624, 812
TscAI CASTG 1 cut(s) 958
TseFI GTSAC 3 cut(s) 110, 574, 781
TseI GCWGC 2 cut(s) 536, 613
Tsp45I GTSAC 3 cut(s) 110, 574, 781
TspDTI ATGAA 7 cut(s) 37, 45, 164, 261, 284, 471, 812
TspGWI ACGGA 1 cut(s) 684
TspRI CASTG 1 cut(s) 958
VpaK11BI GGWCC 1 cut(s) 602
XapI RAATTY 2 cut(s) 365, 545
XmnI GAANNNNTTC 2 cut(s) 171, 977
XspI CTAG 1 cut(s) 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.