Rh1DG045600

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000709
Physical Location & Seq
Forward (+)
7472688 .. 7474694
2007 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG045600.1

Sequence Viewer

Length: 948 bp
ATGAGTGGAGAAAGGAAGGTTGTGTGTGTTACAGGAGCTTCTGGTTTCATAGCTTCATGGCTGGTGAAGCTCTTGTTACAGCGAAGTTATGTCGTCAGAGCCACCGTTCGTGACCCAAATAATCCGAAGAAAACAGAACACTTGCTCTCACTTGAGGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAGGGGTCTTTTGACCTTGTAGTTGATGGATGTGAAGGTGTTTTCCACACAGCATCTCCGGCAGAACTGATTGATCCTGCAGTAAAGGGAACGCTTAATGTTCTTAAATCATGCGGGAAATTTCCAACAATCAAGAGAGTAGTTTTGACATCTTCCATGGCGGCAGTTATGGTCAATGGAAGACCTTTAACCTCTAATGTGATAGTTGATGAAACATGGTTTTCTGATCCACTTGTTTGTGAGGAGTTGAAGGAATGGTATTTTCTTTCGAAAACATTAGCAGAGGAGGCTGCTTGGAAATTTGCTAAGGAAAACGGGATCGACATGGTGACCATAAATCCCTCGTATGTACTGGGTCCATTCTTGCAGCCAACTCTTAATTTCAGTGTGGAGATGGTTCTGGATCTCAAGAAGGATGTCAATGGAGTAAACAATGCAAATTATACATCTACTGATGTTAGAGATGTTGCCTCGGCTCATATTCAAGCATTTGAAATTCCTTCAGCTAGTGGAAGATATTGTTTAGTTGCAAATGTCACCCCCATTCACGAGGCTCTGAAGATTTTAAAGGAACTTCATCCTACTTTGTGCCCACCTGAAATATGTGAGCATGATATCTCTTCTGCCCCAGAGTATCAAATATCCCAGGAAAAAGCAAAAAGTTTGGGAGTTAGTTTCCTTCCGTTGGAAGTCAGTCTCAGGGACACTATTGAAAGCCTGAAGGAGAAGGGTTTCCTCAAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

315

Amino Acids

34.69

Weight (kDa)

5.6

Isoelectric Point (pI)

27.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 86 2.5e-07 NmrA-like family
Epimerase PF01370 8 - 235 7.5e-20 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 231 1.3e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 186 3.2e-10 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 100 2.2e-07 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 123 1.1e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 315, 362
AclWI GGATC 4 cut(s) 269, 422, 527, 612
AcsI RAATTY 3 cut(s) 320, 500, 696
AcuI CTGAAG 3 cut(s) 687, 779, 941
AfaI GTAC 1 cut(s) 552
AfiI CCNNNNNNNGG 1 cut(s) 886
AgsI TTSAA 5 cut(s) 184, 451, 686, 695, 914
AjnI CCWGG 1 cut(s) 846
AluBI AGCT 4 cut(s) 38, 53, 70, 707
AluI AGCT 4 cut(s) 38, 53, 70, 707
Alw26I GTCTC 1 cut(s) 902
AlwI GGATC 4 cut(s) 269, 422, 527, 612
ApeKI GCWGC 2 cut(s) 491, 568
ApoI RAATTY 3 cut(s) 320, 500, 696
Asp700I GAANNNNTTC 2 cut(s) 171, 932
AspS9I GGNCC 1 cut(s) 557
AsuHPI GGTGA 3 cut(s) 76, 541, 730
AsuII TTCGAA 1 cut(s) 470
AvaII GGWCC 1 cut(s) 557
BaeGI GKGCMC 1 cut(s) 794
BauI CACGAG 1 cut(s) 749
BbsI GAAGAC 1 cut(s) 388
BbvI GCAGC 2 cut(s) 478, 580
BccI CCATC 2 cut(s) 221, 589
BciT130I CCWGG 1 cut(s) 848
BcoDI GTCTC 1 cut(s) 902
BfaI CTAG 1 cut(s) 708
BfmI CTRYAG 1 cut(s) 279
BisI GCNGC 3 cut(s) 363, 492, 569
BlsI GCNGC 3 cut(s) 364, 493, 570
Bme1390I CCNGG 1 cut(s) 848
Bme18I GGWCC 1 cut(s) 557
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 1 cut(s) 558
BmrFI CCNGG 1 cut(s) 848
BmrI ACTGGG 1 cut(s) 563
BmsI GCATC 1 cut(s) 263
BmuI ACTGGG 1 cut(s) 563
BpiI GAAGAC 1 cut(s) 388
Bpu10I CCTNAGC 1 cut(s) 507
Bpu14I TTCGAA 1 cut(s) 470
BpuEI CTTGAG 3 cut(s) 173, 593, 923
BsaJI CCNNGG 3 cut(s) 357, 672, 846
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 886
Bse1I ACTGG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 848
BseDI CCNNGG 3 cut(s) 357, 672, 846
BseGI GGATG 3 cut(s) 236, 622, 778
BseLI CCNNNNNNNGG 1 cut(s) 886
BseMII CTCAG 1 cut(s) 913
BseNI ACTGG 1 cut(s) 558
BseRI GAGGAG 2 cut(s) 458, 500
BseSI GKGCMC 1 cut(s) 794
BseXI GCAGC 2 cut(s) 478, 580
BsiSI CCGG 1 cut(s) 260
BslFI GGGAC 1 cut(s) 917
BslI CCNNNNNNNGG 1 cut(s) 886
BsmAI GTCTC 1 cut(s) 902
BsmFI GGGAC 1 cut(s) 917
Bsp119I TTCGAA 1 cut(s) 470
Bsp1286I GDGCHC 1 cut(s) 794
Bsp143I GATC 4 cut(s) 274, 427, 519, 604
Bsp19I CCATGG 1 cut(s) 357
BspACI CCGC 2 cut(s) 315, 362
BspCNI CTCAG 1 cut(s) 912
BspLI GGNNCC 1 cut(s) 558
BspMAI CTGCAG 1 cut(s) 283
BspPI GGATC 4 cut(s) 269, 422, 527, 612
BspT104I TTCGAA 1 cut(s) 470
BsrI ACTGG 1 cut(s) 558
BssECI CCNNGG 3 cut(s) 357, 672, 846
BssMI GATC 4 cut(s) 274, 427, 519, 604
BssSI CACGAG 1 cut(s) 749
BssT1I CCWWGG 1 cut(s) 357
Bst2BI CACGAG 1 cut(s) 749
Bst2UI CCWGG 1 cut(s) 848
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 2 cut(s) 195, 826
BstBI TTCGAA 1 cut(s) 470
BstDEI CTNAG 2 cut(s) 507, 899
BstDSI CCRYGG 1 cut(s) 357
BstEII GGTNACC 1 cut(s) 529
BstF5I GGATG 3 cut(s) 236, 622, 778
BstKTI GATC 4 cut(s) 277, 430, 522, 607
BstMAI GTCTC 1 cut(s) 902
BstMBI GATC 4 cut(s) 274, 427, 519, 604
BstMWI GCNNNNNNNGC 3 cut(s) 67, 260, 488
BstNI CCWGG 1 cut(s) 848
BstPI GGTNACC 1 cut(s) 529
BstSCI CCNGG 1 cut(s) 846
BstSFI CTRYAG 1 cut(s) 279
BstSLI GKGCMC 1 cut(s) 794
BstV1I GCAGC 2 cut(s) 478, 580
BstV2I GAAGAC 1 cut(s) 388
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BtgI CCRYGG 1 cut(s) 357
BtsCI GGATG 3 cut(s) 236, 622, 778
BtsIMutI CAGTG 1 cut(s) 592
Cfr13I GGNCC 1 cut(s) 557
Csp6I GTAC 1 cut(s) 551
CviAII CATG 6 cut(s) 57, 312, 358, 417, 526, 812
CviQI GTAC 1 cut(s) 551
DdeI CTNAG 2 cut(s) 507, 899
DpnI GATC 4 cut(s) 276, 429, 521, 606
DpnII GATC 4 cut(s) 274, 427, 519, 604
DraI TTTAAA 1 cut(s) 768
Eam1104I CTCTTC 2 cut(s) 195, 826
EarI CTCTTC 2 cut(s) 195, 826
Eco130I CCWWGG 1 cut(s) 357
Eco32I GATATC 1 cut(s) 817
Eco47I GGWCC 1 cut(s) 557
Eco57I CTGAAG 3 cut(s) 687, 779, 941
Eco91I GGTNACC 1 cut(s) 529
EcoO65I GGTNACC 1 cut(s) 529
EcoRII CCWGG 1 cut(s) 846
EcoRV GATATC 1 cut(s) 817
EcoT14I CCWWGG 1 cut(s) 357
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 6 cut(s) 60, 315, 361, 420, 529, 815
FaqI GGGAC 1 cut(s) 917
FatI CATG 6 cut(s) 56, 311, 357, 416, 525, 811
FauI CCCGC 1 cut(s) 308
Fnu4HI GCNGC 3 cut(s) 363, 492, 569
FokI GGATG 3 cut(s) 243, 629, 765
Fsp4HI GCNGC 3 cut(s) 363, 492, 569
FspBI CTAG 1 cut(s) 708
GluI GCNGC 3 cut(s) 363, 492, 569
HapII CCGG 1 cut(s) 260
Hin1II CATG 6 cut(s) 60, 315, 361, 420, 529, 815
HpaII CCGG 1 cut(s) 260
HphI GGTGA 3 cut(s) 76, 541, 730
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 4 cut(s) 98, 126, 427, 759
Hpy188III TCNNGA 5 cut(s) 110, 334, 602, 610, 749
Hpy8I GTNNAC 1 cut(s) 631
HpyAV CCTTC 8 cut(s) 10, 230, 445, 607, 711, 890, 916, 922
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4V TGCA 4 cut(s) 281, 568, 638, 731
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 260, 488
HpyF3I CTNAG 2 cut(s) 507, 899
Hsp92II CATG 6 cut(s) 60, 315, 361, 420, 529, 815
Kzo9I GATC 4 cut(s) 274, 427, 519, 604
LmnI GCTCC 2 cut(s) 35, 158
Lsp1109I GCAGC 2 cut(s) 478, 580
LweI GCATC 1 cut(s) 263
MaeI CTAG 1 cut(s) 708
MaeIII GTNAC 5 cut(s) 28, 75, 110, 529, 736
MalI GATC 4 cut(s) 276, 429, 521, 606
MboI GATC 4 cut(s) 274, 427, 519, 604
MboII GAAGA 7 cut(s) 139, 212, 345, 393, 726, 772, 813
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 1 cut(s) 794
MluCI AATT 5 cut(s) 320, 500, 580, 640, 696
MmeI TCCRAC 2 cut(s) 350, 867
MroXI GAANNNNTTC 2 cut(s) 171, 932
MseI TTAA 5 cut(s) 297, 306, 389, 579, 767
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 848
MvaI CCWGG 1 cut(s) 848
MwoI GCNNNNNNNGC 3 cut(s) 67, 260, 488
NcoI CCATGG 1 cut(s) 357
NdeII GATC 4 cut(s) 274, 427, 519, 604
NlaIII CATG 6 cut(s) 60, 315, 361, 420, 529, 815
NlaIV GGNNCC 1 cut(s) 558
NmeAIII GCCGAG 1 cut(s) 653
NmuCI GTSAC 3 cut(s) 110, 529, 736
NspV TTCGAA 1 cut(s) 470
PdmI GAANNNNTTC 2 cut(s) 171, 932
PkrI GCNGC 3 cut(s) 364, 493, 570
Psp6I CCWGG 1 cut(s) 846
PspEI GGTNACC 1 cut(s) 529
PspGI CCWGG 1 cut(s) 846
PspN4I GGNNCC 1 cut(s) 558
PspPI GGNCC 1 cut(s) 557
PstI CTGCAG 1 cut(s) 283
PsuI RGATCY 1 cut(s) 604
RsaI GTAC 1 cut(s) 552
RsaNI GTAC 1 cut(s) 551
SaqAI TTAA 5 cut(s) 297, 306, 389, 579, 767
SatI GCNGC 3 cut(s) 363, 492, 569
Sau3AI GATC 4 cut(s) 274, 427, 519, 604
Sau96I GGNCC 1 cut(s) 557
ScrFI CCNGG 1 cut(s) 848
SduI GDGCHC 1 cut(s) 794
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 1 cut(s) 279
SfuI TTCGAA 1 cut(s) 470
SinI GGWCC 1 cut(s) 557
SmlI CTYRAG 3 cut(s) 152, 608, 938
SmoI CTYRAG 3 cut(s) 152, 608, 938
Sse9I AATT 5 cut(s) 320, 500, 580, 640, 696
SsiI CCGC 2 cut(s) 315, 362
SspMI CTAG 1 cut(s) 708
StyD4I CCNGG 1 cut(s) 846
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 1 cut(s) 106
TaqI TCGA 2 cut(s) 470, 522
TasI AATT 5 cut(s) 320, 500, 580, 640, 696
TatI WGTACW 1 cut(s) 550
TauI GCSGC 1 cut(s) 365
Tru1I TTAA 5 cut(s) 297, 306, 389, 579, 767
Tru9I TTAA 5 cut(s) 297, 306, 389, 579, 767
TscAI CASTG 1 cut(s) 592
TseFI GTSAC 3 cut(s) 110, 529, 736
TseI GCWGC 2 cut(s) 491, 568
Tsp45I GTSAC 3 cut(s) 110, 529, 736
TspDTI ATGAA 5 cut(s) 37, 45, 164, 426, 767
TspGWI ACGGA 1 cut(s) 873
TspRI CASTG 1 cut(s) 592
VpaK11BI GGWCC 1 cut(s) 557
XapI RAATTY 3 cut(s) 320, 500, 696
XmnI GAANNNNTTC 2 cut(s) 171, 932
XspI CTAG 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.