Rw1G002030

reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000709
Physical Location & Seq
Reverse (-)
3623640 .. 3626149
2510 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G002030.1

Sequence Viewer

Length: 987 bp
ATGAGTGGAGAAACTAAGGTTGTGTGTGTAACAGGAGCCTCCGGTTACATAGCATCGTGGCTAGTGAAGCTCTTATTACAAGAAGGTTATACTGTCAAAGCCACCGTTCGTGACCCAAATGATGCAAAGAAAACAGAACACTTGCTTTCACTTGATGGAGCTAAGGAAAGGCTTCATCTGTTCAAAGCAGATTTATTAGAAGAAGGATCTTTTGACCCTGTCGTTGATGGATGCGAAGGTGTTTTTCATACAGCATCCCCTGTCCTATTGTCATCTACTAATCCGCAGGCAGAACTAATTGACCCTGCAGTAAAGGGAACACATAATGTTCTGAAATCATGTGCAAAATTTCCGACTGTCAAGAGAGTGGTTTTAACTTCTTCTATGGCTGCAGTAATATTGACCGGAAAACCTCTGACCTCAGATGTGGTAGTTGATGAAACATGGTTTTCGGATCCACTTTTTTGTGAGAAGAATAAGCTATGGTATATTCTTTCAAAAACTATAGCTGAGGATGCTGCTTGGAAATTCGCCAAGGAAAATGGGATTGATCTGGTTTCAATAAATCCAGGGTTTGTGATTGGTCCGTTGTTACAGCCGACTCTCAATCTCTCTGTGGAAGTGATTCAGAGTCTCCTAACTGGCAAGGCATTGCCCGCTTCAAATTACAGATTCGTTGATGTTAGAGATGTTGCCTCTGCGCATATTCAAGCCTTTGAAGTTCCTTCAGCCAGTGGAAGGTATTGTTTGGTCAGCCATGTTGCACACATTTCCGAGGTTCTGAAGATTATAAGACAATTTTATCCCACTTTGTACATTCCGGAAAGATATGAGGGTGACAATAATCCTCATCGTATTCTTCCTTCTGACCCAAGTTATCAAGTATCCAACGAGAAGGCAAAAGGTTTAGGAATTAACTTCTTTCCTTTTGAAGTAACGGTCAAAGACACTGTTGAAAGCCTGAAGGAGAAGGGCTTCCTCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

328

Amino Acids

36.01

Weight (kDa)

5.94

Isoelectric Point (pI)

27.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 104 1.3e-09 NmrA-like family
Epimerase PF01370 8 - 244 1.7e-22 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 238 1.6e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 199 1.4e-11 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 128 5.2e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 131 1.5e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 791
Acc16I TGCGCA 1 cut(s) 702
AccIII TCCGGA 1 cut(s) 820
AciI CCGC 2 cut(s) 284, 657
AclWI GGATC 3 cut(s) 214, 449, 462
AcsI RAATTY 2 cut(s) 347, 527
AcuI CTGAAG 3 cut(s) 711, 803, 983
AfaI GTAC 1 cut(s) 815
AfiI CCNNNNNNNGG 1 cut(s) 738
AgsI TTSAA 8 cut(s) 184, 498, 561, 663, 710, 719, 932, 956
AjnI CCWGG 1 cut(s) 568
AluBI AGCT 4 cut(s) 70, 161, 481, 509
AluI AGCT 4 cut(s) 70, 161, 481, 509
Alw26I GTCTC 1 cut(s) 638
AlwI GGATC 3 cut(s) 214, 449, 462
Aor13HI TCCGGA 1 cut(s) 820
ApeKI GCWGC 2 cut(s) 389, 518
ApoI RAATTY 2 cut(s) 347, 527
Asp700I GAANNNNTTC 3 cut(s) 171, 624, 974
AspLEI GCGC 1 cut(s) 703
AspS9I GGNCC 1 cut(s) 584
AsuHPI GGTGA 1 cut(s) 848
AvaII GGWCC 1 cut(s) 584
BamHI GGATCC 1 cut(s) 454
BarI GAAGNNNNNNTAC 2 cut(s) 847, 879
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 2 cut(s) 376, 505
BccI CCATC 2 cut(s) 149, 221
BciT130I CCWGG 1 cut(s) 570
BciVI GTATCC 1 cut(s) 895
BcoDI GTCTC 1 cut(s) 638
BfaI CTAG 1 cut(s) 62
BfmI CTRYAG 3 cut(s) 306, 390, 504
BfuI GTATCC 1 cut(s) 895
BisI GCNGC 2 cut(s) 390, 519
BlsI GCNGC 2 cut(s) 391, 520
Bme1390I CCNGG 1 cut(s) 570
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 1 cut(s) 584
BmiI GGNNCC 2 cut(s) 37, 456
BmrFI CCNGG 1 cut(s) 570
BmsI GCATC 5 cut(s) 62, 112, 221, 263, 505
Bpu10I CCTNAGC 2 cut(s) 162, 510
BsaJI CCNNGG 3 cut(s) 534, 569, 774
BsaWI WCCGGW 3 cut(s) 41, 404, 820
Bsc4I CCNNNNNNNGG 1 cut(s) 738
Bse1I ACTGG 2 cut(s) 646, 732
Bse3DI GCAATG 1 cut(s) 650
BseAI TCCGGA 1 cut(s) 820
BseBI CCWGG 1 cut(s) 570
BseDI CCNNGG 3 cut(s) 534, 569, 774
BseGI GGATG 3 cut(s) 236, 254, 520
BseLI CCNNNNNNNGG 1 cut(s) 738
BseMI GCAATG 1 cut(s) 650
BseMII CTCAG 2 cut(s) 435, 501
BseNI ACTGG 2 cut(s) 646, 732
BseXI GCAGC 2 cut(s) 376, 505
BsiSI CCGG 3 cut(s) 42, 405, 821
BslI CCNNNNNNNGG 1 cut(s) 738
BsmAI GTCTC 1 cut(s) 638
Bsp13I TCCGGA 1 cut(s) 820
Bsp1407I TGTACA 1 cut(s) 813
Bsp143I GATC 3 cut(s) 206, 454, 550
BspACI CCGC 2 cut(s) 284, 657
BspCNI CTCAG 2 cut(s) 434, 502
BspEI TCCGGA 1 cut(s) 820
BspLI GGNNCC 2 cut(s) 37, 456
BspMAI CTGCAG 2 cut(s) 310, 394
BspPI GGATC 3 cut(s) 214, 449, 462
BsrDI GCAATG 1 cut(s) 650
BsrGI TGTACA 1 cut(s) 813
BsrI ACTGG 2 cut(s) 646, 732
BssECI CCNNGG 3 cut(s) 534, 569, 774
BssMI GATC 3 cut(s) 206, 454, 550
BssT1I CCWWGG 1 cut(s) 534
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 5 cut(s) 94, 106, 358, 940, 952
BstAUI TGTACA 1 cut(s) 813
BstC8I GCNNGC 2 cut(s) 288, 657
BstDEI CTNAG 4 cut(s) 15, 162, 421, 510
BstF5I GGATG 3 cut(s) 236, 254, 520
BstHHI GCGC 1 cut(s) 703
BstKTI GATC 3 cut(s) 209, 457, 553
BstMAI GTCTC 1 cut(s) 638
BstMBI GATC 3 cut(s) 206, 454, 550
BstMWI GCNNNNNNNGC 3 cut(s) 67, 515, 656
BstNI CCWGG 1 cut(s) 570
BstSCI CCNGG 1 cut(s) 568
BstSFI CTRYAG 3 cut(s) 306, 390, 504
BstV1I GCAGC 2 cut(s) 376, 505
BstX2I RGATCY 2 cut(s) 206, 454
BstYI RGATCY 2 cut(s) 206, 454
BsuI GTATCC 1 cut(s) 895
BtsCI GGATG 3 cut(s) 236, 254, 520
BtsIMutI CAGTG 2 cut(s) 739, 948
Cac8I GCNNGC 2 cut(s) 288, 657
CfoI GCGC 1 cut(s) 703
Cfr13I GGNCC 1 cut(s) 584
Csp6I GTAC 1 cut(s) 814
CviAII CATG 3 cut(s) 339, 444, 758
CviQI GTAC 1 cut(s) 814
DdeI CTNAG 4 cut(s) 15, 162, 421, 510
DpnI GATC 3 cut(s) 208, 456, 552
DpnII GATC 3 cut(s) 206, 454, 550
Eco130I CCWWGG 1 cut(s) 534
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 3 cut(s) 711, 803, 983
EcoRII CCWGG 1 cut(s) 568
EcoT14I CCWWGG 1 cut(s) 534
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 3 cut(s) 342, 447, 761
FatI CATG 3 cut(s) 338, 443, 757
FauI CCCGC 1 cut(s) 664
Fnu4HI GCNGC 2 cut(s) 390, 519
FokI GGATG 3 cut(s) 241, 243, 527
Fsp4HI GCNGC 2 cut(s) 390, 519
FspBI CTAG 1 cut(s) 62
FspI TGCGCA 1 cut(s) 702
GlaI GCGC 1 cut(s) 702
GluI GCNGC 2 cut(s) 390, 519
HapII CCGG 3 cut(s) 42, 405, 821
HhaI GCGC 1 cut(s) 703
Hin1II CATG 3 cut(s) 342, 447, 761
Hin6I GCGC 1 cut(s) 701
HinP1I GCGC 1 cut(s) 701
HinfI GANTC 4 cut(s) 601, 625, 631, 672
HpaII CCGG 3 cut(s) 42, 405, 821
HphI GGTGA 1 cut(s) 848
Hpy188I TCNGA 9 cut(s) 333, 354, 417, 424, 454, 630, 775, 783, 868
Hpy188III TCNNGA 3 cut(s) 110, 361, 821
HpyAV CCTTC 9 cut(s) 77, 197, 230, 732, 735, 873, 889, 958, 964
HpyCH4III ACNGT 5 cut(s) 94, 106, 358, 940, 952
HpyCH4V TGCA 5 cut(s) 125, 308, 344, 392, 764
HpyF10VI GCNNNNNNNGC 3 cut(s) 67, 515, 656
HpyF3I CTNAG 4 cut(s) 15, 162, 421, 510
Hsp92II CATG 3 cut(s) 342, 447, 761
HspAI GCGC 1 cut(s) 701
Kpn2I TCCGGA 1 cut(s) 820
Kzo9I GATC 3 cut(s) 206, 454, 550
LmnI GCTCC 2 cut(s) 35, 158
Lsp1109I GCAGC 2 cut(s) 376, 505
LweI GCATC 5 cut(s) 62, 112, 221, 263, 505
MaeI CTAG 1 cut(s) 62
MaeIII GTNAC 6 cut(s) 28, 44, 110, 591, 836, 934
MalI GATC 3 cut(s) 208, 456, 552
MboI GATC 3 cut(s) 206, 454, 550
MboII GAAGA 5 cut(s) 212, 372, 484, 796, 851
MflI RGATCY 2 cut(s) 206, 454
MluCI AATT 7 cut(s) 297, 347, 527, 664, 797, 912, 982
MlyI GAGTC 2 cut(s) 595, 640
MmeI TCCRAC 2 cut(s) 377, 912
MnlI CCTC 8 cut(s) 49, 423, 430, 505, 706, 769, 826, 858
MroI TCCGGA 1 cut(s) 820
MroXI GAANNNNTTC 3 cut(s) 171, 624, 974
MseI TTAA 2 cut(s) 374, 915
MspI CCGG 3 cut(s) 42, 405, 821
MspR9I CCNGG 1 cut(s) 570
MvaI CCWGG 1 cut(s) 570
MwoI GCNNNNNNNGC 3 cut(s) 67, 515, 656
NdeII GATC 3 cut(s) 206, 454, 550
NlaIII CATG 3 cut(s) 342, 447, 761
NlaIV GGNNCC 2 cut(s) 37, 456
NmuCI GTSAC 2 cut(s) 110, 836
NsbI TGCGCA 1 cut(s) 702
PdmI GAANNNNTTC 3 cut(s) 171, 624, 974
PfeI GAWTC 2 cut(s) 625, 672
PflFI GACNNNGTC 1 cut(s) 218
PkrI GCNGC 2 cut(s) 391, 520
PleI GAGTC 2 cut(s) 595, 639
PpsI GAGTC 2 cut(s) 595, 639
PsiI TTATAA 1 cut(s) 791
Psp6I CCWGG 1 cut(s) 568
PspGI CCWGG 1 cut(s) 568
PspN4I GGNNCC 2 cut(s) 37, 456
PspPI GGNCC 1 cut(s) 584
PstI CTGCAG 2 cut(s) 310, 394
PsuI RGATCY 2 cut(s) 206, 454
PsyI GACNNNGTC 1 cut(s) 218
RsaI GTAC 1 cut(s) 815
RsaNI GTAC 1 cut(s) 814
SaqAI TTAA 2 cut(s) 374, 915
SatI GCNGC 2 cut(s) 390, 519
Sau3AI GATC 3 cut(s) 206, 454, 550
Sau96I GGNCC 1 cut(s) 584
SchI GAGTC 2 cut(s) 595, 640
ScrFI CCNGG 1 cut(s) 570
SfaNI GCATC 5 cut(s) 62, 112, 221, 263, 505
SfcI CTRYAG 3 cut(s) 306, 390, 504
SinI GGWCC 1 cut(s) 584
Sse9I AATT 7 cut(s) 297, 347, 527, 664, 797, 912, 982
SsiI CCGC 2 cut(s) 284, 657
SspI AATATT 1 cut(s) 399
SspMI CTAG 1 cut(s) 62
StyD4I CCNGG 1 cut(s) 568
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 5 cut(s) 94, 106, 358, 940, 952
TasI AATT 7 cut(s) 297, 347, 527, 664, 797, 912, 982
TatI WGTACW 1 cut(s) 813
TfiI GAWTC 2 cut(s) 625, 672
Tru1I TTAA 2 cut(s) 374, 915
Tru9I TTAA 2 cut(s) 374, 915
TscAI CASTG 2 cut(s) 739, 955
TseFI GTSAC 2 cut(s) 110, 836
TseI GCWGC 2 cut(s) 389, 518
Tsp45I GTSAC 2 cut(s) 110, 836
TspDTI ATGAA 3 cut(s) 164, 236, 453
TspGWI ACGGA 1 cut(s) 576
TspRI CASTG 2 cut(s) 739, 955
Tth111I GACNNNGTC 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 584
XapI RAATTY 2 cut(s) 347, 527
XmnI GAANNNNTTC 3 cut(s) 171, 624, 974
XspI CTAG 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.