RLG00000030595

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000709
Physical Location & Seq
Forward (+)
64204973 .. 64208676
3704 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030595

Sequence Viewer

Length: 1209 bp
ATGAGTGGAGAAACTAAGGTTGTGTGTGTAACAGGAGCCTCCGGTTACATAGCATCGTGGCTGGTGAAGCTCTTATTACAACAAGGTTATACTGTCAAAGCCACCGTTCGTGACCCAAATGATGCAAAGAAAACAGAACACTTGCTTTCACTTGATGGAGCTAAGGAAAGGCTTCATCTGTTCAAAGCAGATTTATTAGAAGAAGGATCTTTTGACCCTGTCGTTGATGGATGTGAAGGTGTTTTTCATACAGCATCCCCTGTGCTATTCTCATCTACTAATCCGCAGGCAGAACTAATTGATCCTGCAGTAAAGGGAACACATAATGTTCTGAAATCATGCGCAAAATTTCCTACTGTCAAGAGAGTGGTTTTAACTTCTTCTATGGCTTCAGTAATATTGACCGGAAAACCTCTGACCTCAGATGTGGTAGTTGATGAAACATGGTTTTCGGATCCACTTTTTTGTGAGAAGAATAAGCTATGGTATATTCTTTCAAAAACTTTAGCTGAGGAGGCTGCTTGGAAATTCGCCAAGGAAAATGGGATTGATCTGGTTTCAATAAATCCAGGGTTTGTGATTGGTCCGTTGTTGCAGCCGACTCTGAATCTCTCTGTGGAGGTGATTCAGAGTCTCCTAACTGGCAAGGCATTGCCCGCTTCAAATTACAGATTCATTGATGTTAGAGATGTTGCCTCTGCGCATATTCAAGCCTTCGAAGTTCCTTCAGCCAGTGGAAGGTATTGTTTGGTCAGCCATGTTGCGCACATTTCCGAGGCCCTGAAGATTATAAGACAACTTTATCCCACTTTGTACATTCCTGAAAGATATAAGGATGACAATAATCCTCATCGTATTCTTCCTTCTGACCCAAGTTATAAAGTATCCAATGAGAAGGCAAAAGGAAACCCTTCTCTCTCTCCTCTTCTCCTTTCTCCGGATACGATCGACGACTTCGAGAGGGGCGTAGAGCGGAGCCCTCTCTCTCCTGCCGCCCAAAAATCTCCGCCGTTGATTCAATTCCGGGTTCATGTCATCTCATCTCAGATCTACTTGCAAATGGCTCAGATCTACCTCCATCTTAACCTCCTTGGCGGCTCTCTCACAGACCAAAGGAAGCCATGGCCGCCGTGCATGTCGTATCCTGCCCTCCCCGGCTTGGCAGCGAATCGAATTAGGTCGAGTGTATTCTCGCATAGATTCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

403

Amino Acids

44.26

Weight (kDa)

8.11

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 104 1.8e-09 NmrA-like family
Epimerase PF01370 8 - 244 1.1e-22 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 238 3.1e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 199 7.4e-12 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 128 1.2e-10 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 130 2.8e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 791, 879
Acc16I TGCGCA 3 cut(s) 343, 702, 765
AccBSI CCGCTC 1 cut(s) 973
AccIII TCCGGA 1 cut(s) 937
AciI CCGC 7 cut(s) 284, 657, 973, 993, 1007, 1095, 1127
AclWI GGATC 4 cut(s) 214, 296, 449, 462
AcoI YGGCCR 1 cut(s) 1124
AcsI RAATTY 2 cut(s) 347, 527
AcuI CTGAAG 3 cut(s) 375, 711, 803
AfaI GTAC 1 cut(s) 815
AfiI CCNNNNNNNGG 3 cut(s) 738, 937, 1159
AgsI TTSAA 6 cut(s) 184, 498, 561, 663, 710, 1019
AjnI CCWGG 1 cut(s) 568
AluBI AGCT 4 cut(s) 70, 161, 481, 509
AluI AGCT 4 cut(s) 70, 161, 481, 509
Alw26I GTCTC 1 cut(s) 638
AlwI GGATC 4 cut(s) 214, 296, 449, 462
Aor13HI TCCGGA 1 cut(s) 937
AoxI GGCC 2 cut(s) 777, 1124
ApeKI GCWGC 3 cut(s) 518, 595, 1163
ApoI RAATTY 2 cut(s) 347, 527
Asp700I GAANNNNTTC 2 cut(s) 171, 910
AspLEI GCGC 3 cut(s) 344, 703, 766
AspS9I GGNCC 2 cut(s) 584, 778
AsuC2I CCSGG 2 cut(s) 1025, 1155
AsuHPI GGTGA 2 cut(s) 76, 634
AsuII TTCGAA 1 cut(s) 717
AvaII GGWCC 1 cut(s) 584
BamHI GGATCC 1 cut(s) 454
BanII GRGCYC 1 cut(s) 980
BarI GAAGNNNNNNTAC 2 cut(s) 847, 879
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 3 cut(s) 505, 607, 1175
BccI CCATC 3 cut(s) 149, 221, 1086
BceAI ACGGC 2 cut(s) 994, 1114
BciT130I CCWGG 1 cut(s) 570
BciVI GTATCC 3 cut(s) 895, 934, 1152
BcnI CCSGG 2 cut(s) 1025, 1155
BcoDI GTCTC 1 cut(s) 638
BfmI CTRYAG 1 cut(s) 306
BfuI GTATCC 3 cut(s) 895, 934, 1152
BglII AGATCT 2 cut(s) 1047, 1068
BisI GCNGC 6 cut(s) 519, 596, 993, 1096, 1127, 1164
BlsI GCNGC 6 cut(s) 520, 597, 994, 1097, 1128, 1165
Bme1390I CCNGG 3 cut(s) 570, 1025, 1155
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 2 cut(s) 584, 778
BmiI GGNNCC 3 cut(s) 37, 456, 977
BmrFI CCNGG 3 cut(s) 570, 1025, 1155
BmsI GCATC 3 cut(s) 62, 112, 263
Bpu10I CCTNAGC 2 cut(s) 162, 510
Bpu14I TTCGAA 1 cut(s) 717
BpuMI CCSGG 2 cut(s) 1025, 1155
BsaJI CCNNGG 6 cut(s) 534, 569, 774, 1090, 1121, 1153
BsaWI WCCGGW 3 cut(s) 41, 404, 937
Bsc4I CCNNNNNNNGG 3 cut(s) 738, 937, 1159
Bse1I ACTGG 2 cut(s) 646, 732
Bse3DI GCAATG 1 cut(s) 650
BseAI TCCGGA 1 cut(s) 937
BseBI CCWGG 1 cut(s) 570
BseDI CCNNGG 6 cut(s) 534, 569, 774, 1090, 1121, 1153
BseGI GGATG 3 cut(s) 236, 254, 841
BseLI CCNNNNNNNGG 3 cut(s) 738, 937, 1159
BseMI GCAATG 1 cut(s) 650
BseMII CTCAG 4 cut(s) 435, 501, 1058, 1079
BseNI ACTGG 2 cut(s) 646, 732
BseRI GAGGAG 2 cut(s) 527, 912
BseXI GCAGC 3 cut(s) 505, 607, 1175
Bsh1285I CGRYCG 1 cut(s) 948
BshFI GGCC 2 cut(s) 779, 1126
BsiEI CGRYCG 1 cut(s) 948
BsiSI CCGG 5 cut(s) 42, 405, 938, 1024, 1155
BslI CCNNNNNNNGG 3 cut(s) 738, 937, 1159
BsmAI GTCTC 1 cut(s) 638
BsnI GGCC 2 cut(s) 779, 1126
Bsp119I TTCGAA 1 cut(s) 717
Bsp1286I GDGCHC 1 cut(s) 980
Bsp13I TCCGGA 1 cut(s) 937
Bsp1407I TGTACA 1 cut(s) 813
Bsp143I GATC 7 cut(s) 206, 301, 454, 550, 945, 1047, 1068
Bsp19I CCATGG 1 cut(s) 1121
BspACI CCGC 7 cut(s) 284, 657, 973, 993, 1007, 1095, 1127
BspANI GGCC 2 cut(s) 779, 1126
BspCNI CTCAG 4 cut(s) 434, 502, 1057, 1078
BspEI TCCGGA 1 cut(s) 937
BspLI GGNNCC 3 cut(s) 37, 456, 977
BspMAI CTGCAG 1 cut(s) 310
BspPI GGATC 4 cut(s) 214, 296, 449, 462
BspT104I TTCGAA 1 cut(s) 717
BsrBI CCGCTC 1 cut(s) 973
BsrDI GCAATG 1 cut(s) 650
BsrGI TGTACA 1 cut(s) 813
BsrI ACTGG 2 cut(s) 646, 732
BssECI CCNNGG 6 cut(s) 534, 569, 774, 1090, 1121, 1153
BssMI GATC 7 cut(s) 206, 301, 454, 550, 945, 1047, 1068
BssT1I CCWWGG 3 cut(s) 534, 1090, 1121
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 3 cut(s) 94, 106, 358
Bst6I CTCTTC 1 cut(s) 930
BstAUI TGTACA 1 cut(s) 813
BstBI TTCGAA 1 cut(s) 717
BstC8I GCNNGC 2 cut(s) 288, 657
BstDEI CTNAG 6 cut(s) 15, 162, 421, 510, 1044, 1065
BstDSI CCRYGG 1 cut(s) 1121
BstF5I GGATG 3 cut(s) 236, 254, 841
BstHHI GCGC 3 cut(s) 344, 703, 766
BstKTI GATC 7 cut(s) 209, 304, 457, 553, 948, 1050, 1071
BstMAI GTCTC 1 cut(s) 638
BstMBI GATC 7 cut(s) 206, 301, 454, 550, 945, 1047, 1068
BstMCI CGRYCG 1 cut(s) 948
BstMWI GCNNNNNNNGC 4 cut(s) 67, 515, 656, 1126
BstNI CCWGG 1 cut(s) 570
BstNSI RCATGY 1 cut(s) 1138
BstSCI CCNGG 3 cut(s) 568, 1023, 1153
BstSFI CTRYAG 1 cut(s) 306
BstV1I GCAGC 3 cut(s) 505, 607, 1175
BstX2I RGATCY 4 cut(s) 206, 454, 1047, 1068
BstYI RGATCY 4 cut(s) 206, 454, 1047, 1068
BsuI GTATCC 3 cut(s) 895, 934, 1152
BsuRI GGCC 2 cut(s) 779, 1126
BtgI CCRYGG 1 cut(s) 1121
BtsCI GGATG 3 cut(s) 236, 254, 841
BtsIMutI CAGTG 1 cut(s) 739
Cac8I GCNNGC 2 cut(s) 288, 657
CfoI GCGC 3 cut(s) 344, 703, 766
Cfr13I GGNCC 2 cut(s) 584, 778
Csp6I GTAC 1 cut(s) 814
CviAII CATG 7 cut(s) 339, 444, 758, 1031, 1122, 1135, 1206
CviQI GTAC 1 cut(s) 814
DdeI CTNAG 6 cut(s) 15, 162, 421, 510, 1044, 1065
DpnI GATC 7 cut(s) 208, 303, 456, 552, 947, 1049, 1070
DpnII GATC 7 cut(s) 206, 301, 454, 550, 945, 1047, 1068
EaeI YGGCCR 1 cut(s) 1124
Eam1104I CTCTTC 1 cut(s) 930
EarI CTCTTC 1 cut(s) 930
EciI GGCGGA 1 cut(s) 996
Eco130I CCWWGG 3 cut(s) 534, 1090, 1121
Eco24I GRGCYC 1 cut(s) 980
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 3 cut(s) 375, 711, 803
EcoO109I RGGNCCY 1 cut(s) 778
EcoRII CCWGG 1 cut(s) 568
EcoT14I CCWWGG 3 cut(s) 534, 1090, 1121
EcoT38I GRGCYC 1 cut(s) 980
ErhI CCWWGG 3 cut(s) 534, 1090, 1121
FaeI CATG 7 cut(s) 342, 447, 761, 1034, 1125, 1138, 1209
FatI CATG 7 cut(s) 338, 443, 757, 1030, 1121, 1134, 1205
FauI CCCGC 1 cut(s) 664
Fnu4HI GCNGC 6 cut(s) 519, 596, 993, 1096, 1127, 1164
FokI GGATG 3 cut(s) 241, 243, 848
FriOI GRGCYC 1 cut(s) 980
Fsp4HI GCNGC 6 cut(s) 519, 596, 993, 1096, 1127, 1164
FspI TGCGCA 3 cut(s) 343, 702, 765
GlaI GCGC 3 cut(s) 343, 702, 765
GluI GCNGC 6 cut(s) 519, 596, 993, 1096, 1127, 1164
HaeIII GGCC 2 cut(s) 779, 1126
HapII CCGG 5 cut(s) 42, 405, 938, 1024, 1155
HhaI GCGC 3 cut(s) 344, 703, 766
Hin1II CATG 7 cut(s) 342, 447, 761, 1034, 1125, 1138, 1209
Hin6I GCGC 3 cut(s) 342, 701, 764
HinP1I GCGC 3 cut(s) 342, 701, 764
HinfI GANTC 8 cut(s) 601, 607, 625, 631, 672, 1015, 1168, 1200
HpaII CCGG 5 cut(s) 42, 405, 938, 1024, 1155
HphI GGTGA 2 cut(s) 76, 634
Hpy188III TCNNGA 5 cut(s) 110, 361, 821, 938, 958
Hpy99I CGWCG 1 cut(s) 953
HpyAV CCTTC 8 cut(s) 197, 230, 724, 732, 735, 873, 889, 921
HpyCH4III ACNGT 3 cut(s) 94, 106, 358
HpyCH4V TGCA 5 cut(s) 125, 308, 595, 1057, 1134
HpyF10VI GCNNNNNNNGC 4 cut(s) 67, 515, 656, 1126
HpyF3I CTNAG 6 cut(s) 15, 162, 421, 510, 1044, 1065
Hsp92II CATG 7 cut(s) 342, 447, 761, 1034, 1125, 1138, 1209
HspAI GCGC 3 cut(s) 342, 701, 764
Kpn2I TCCGGA 1 cut(s) 937
Kzo9I GATC 7 cut(s) 206, 301, 454, 550, 945, 1047, 1068
LmnI GCTCC 3 cut(s) 35, 158, 975
Lsp1109I GCAGC 3 cut(s) 505, 607, 1175
LweI GCATC 3 cut(s) 62, 112, 263
MaeIII GTNAC 3 cut(s) 28, 44, 110
MalI GATC 7 cut(s) 208, 303, 456, 552, 947, 1049, 1070
MbiI CCGCTC 1 cut(s) 973
MboI GATC 7 cut(s) 206, 301, 454, 550, 945, 1047, 1068
MboII GAAGA 6 cut(s) 212, 372, 484, 796, 851, 917
MflI RGATCY 4 cut(s) 206, 454, 1047, 1068
MhlI GDGCHC 1 cut(s) 980
MluCI AATT 6 cut(s) 297, 347, 527, 664, 1019, 1173
MlyI GAGTC 2 cut(s) 595, 640
MroI TCCGGA 1 cut(s) 937
MroXI GAANNNNTTC 2 cut(s) 171, 910
MseI TTAA 2 cut(s) 374, 1083
MspI CCGG 5 cut(s) 42, 405, 938, 1024, 1155
MspR9I CCNGG 3 cut(s) 570, 1025, 1155
MvaI CCWGG 1 cut(s) 570
MwoI GCNNNNNNNGC 4 cut(s) 67, 515, 656, 1126
NciI CCSGG 2 cut(s) 1025, 1155
NcoI CCATGG 1 cut(s) 1121
NdeII GATC 7 cut(s) 206, 301, 454, 550, 945, 1047, 1068
NlaIII CATG 7 cut(s) 342, 447, 761, 1034, 1125, 1138, 1209
NlaIV GGNNCC 3 cut(s) 37, 456, 977
NmuCI GTSAC 1 cut(s) 110
NsbI TGCGCA 3 cut(s) 343, 702, 765
NspI RCATGY 1 cut(s) 1138
NspV TTCGAA 1 cut(s) 717
PcsI WCGNNNNNNNCGW 2 cut(s) 954, 963
PdmI GAANNNNTTC 2 cut(s) 171, 910
PfeI GAWTC 6 cut(s) 607, 625, 672, 1015, 1168, 1200
PflFI GACNNNGTC 1 cut(s) 218
PkrI GCNGC 6 cut(s) 520, 597, 994, 1097, 1128, 1165
Ple19I CGATCG 1 cut(s) 948
PleI GAGTC 2 cut(s) 595, 639
PpsI GAGTC 2 cut(s) 595, 639
PsiI TTATAA 2 cut(s) 791, 879
Psp6I CCWGG 1 cut(s) 568
PspGI CCWGG 1 cut(s) 568
PspN4I GGNNCC 3 cut(s) 37, 456, 977
PspPI GGNCC 2 cut(s) 584, 778
PstI CTGCAG 1 cut(s) 310
PsuI RGATCY 4 cut(s) 206, 454, 1047, 1068
PsyI GACNNNGTC 1 cut(s) 218
PvuI CGATCG 1 cut(s) 948
RsaI GTAC 1 cut(s) 815
RsaNI GTAC 1 cut(s) 814
SaqAI TTAA 2 cut(s) 374, 1083
SatI GCNGC 6 cut(s) 519, 596, 993, 1096, 1127, 1164
Sau3AI GATC 7 cut(s) 206, 301, 454, 550, 945, 1047, 1068
Sau96I GGNCC 2 cut(s) 584, 778
SchI GAGTC 2 cut(s) 595, 640
ScrFI CCNGG 3 cut(s) 570, 1025, 1155
SduI GDGCHC 1 cut(s) 980
SfaNI GCATC 3 cut(s) 62, 112, 263
SfcI CTRYAG 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 717
SinI GGWCC 1 cut(s) 584
Sse9I AATT 6 cut(s) 297, 347, 527, 664, 1019, 1173
SsiI CCGC 7 cut(s) 284, 657, 973, 993, 1007, 1095, 1127
SspI AATATT 1 cut(s) 399
StyD4I CCNGG 3 cut(s) 568, 1023, 1153
StyI CCWWGG 3 cut(s) 534, 1090, 1121
TaaI ACNGT 3 cut(s) 94, 106, 358
TaqI TCGA 5 cut(s) 717, 948, 957, 1171, 1181
TasI AATT 6 cut(s) 297, 347, 527, 664, 1019, 1173
TatI WGTACW 1 cut(s) 813
TauI GCSGC 3 cut(s) 995, 1098, 1129
TfiI GAWTC 6 cut(s) 607, 625, 672, 1015, 1168, 1200
Tru1I TTAA 2 cut(s) 374, 1083
Tru9I TTAA 2 cut(s) 374, 1083
TscAI CASTG 1 cut(s) 739
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 3 cut(s) 518, 595, 1163
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 5 cut(s) 164, 236, 453, 664, 1019
TspGWI ACGGA 1 cut(s) 576
TspRI CASTG 1 cut(s) 739
Tth111I GACNNNGTC 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 584
XapI RAATTY 2 cut(s) 347, 527
XceI RCATGY 1 cut(s) 1138
XmnI GAANNNNTTC 2 cut(s) 171, 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.