MD02G1304900.v1.1

reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000709
Physical Location & Seq
Forward (+)
35818328 .. 35822062
3735 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1304900.v1.1.491

Sequence Viewer

Length: 981 bp
ATGAGTGGAGGAACAAAGGTGGTATGTGTAACAGGAGCCTCAGGTTACATAGCATCATGGTTGGTCAAACTCTTATTACAAAGGGGTTATACCGTCAAAGCCACTGTTCGTGATCCAAGTGATTCAAAGAAAACAGAACACTTGCTCTCACTAGATGGAGCAAAGGAAAGGCTTCGTTTGTTCAAAGCAGATTTATTAGAAGAAGGATCTTTTGACACCGTAGTTGACGGATGTCAAGGCGTTTTTCATACAGCATCTCCTGTTCAGTTTTCAGTCACTGACCCACAGGCAGAACTAGTTGAGCCTGCAGTGAAGGGAACACTTAATGTTCTAAAATCATGCGCGAAATTTCCTACTGTCAAGAGAGTGGTTTTAACATCTTCTATGGCTTCAGTATCGGTGAATGGGAAACCTCTGACATCTGATGTGGTTTTGGATGAAACATGGTATTCGGATCCACTTGTTTGTGAGAAGAACGAGAGATGGTATGCGCTCTCAAAAACTTTAGCTGAGGAGGCTGCCTGGAAATACGCTAAAGAAAATGGAATTGACTTGGTTACAATGAATCCAGGGTTTGTGATAGGTCCACTCTTACAGCCAACACTTAATCTCACTGTGGAGATGATTCAGAATCTCATAACCGGTACAGGTACCAAAACACTACCCCTTTCAAATTACCGGTCCATTGATGTTAGGGATGTTGTCTCTGCTCATATTCTGGCATTTGAAGTTCCTTCAGCAAGTGGAAGATATTGTTTAGTTTCAAATGTTACACACGCTTCCGAGGTTCTGAAGATTATACAAGACCTTTATCCCACTTTGCACCTTCCTGAAAAATGTGAGATTGGTAGTAGTTCAGACCCGAAATATCCAATATCAGCAGAGAAAGCAAAAGGTTTAGGAATTAATTTCCTTCCGTTGGAAGTCAGTCTTAGAGACACAATTGAAAGCCTGAAGGAGAAGGGCTTCCTCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

327

Amino Acids

35.5

Weight (kDa)

6.25

Isoelectric Point (pI)

28.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 101 4.3e-10 NmrA-like family
Epimerase PF01370 8 - 247 2.8e-26 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 242 1.4e-18 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 245 7.1e-16 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 228 2.1e-15 Male sterility protein
NAD_binding_10 PF13460 12 - 136 3.2e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 650
AccB1I GGYRCC 1 cut(s) 650
AccII CGCG 1 cut(s) 344
AclWI GGATC 4 cut(s) 107, 214, 449, 462
AcsI RAATTY 1 cut(s) 347
AcuI CTGAAG 4 cut(s) 375, 720, 812, 974
AfaI GTAC 2 cut(s) 646, 652
AfiI CCNNNNNNNGG 1 cut(s) 919
AgeI ACCGGT 2 cut(s) 641, 678
AgsI TTSAA 6 cut(s) 126, 184, 672, 728, 765, 947
AhlI ACTAGT 1 cut(s) 295
AjnI CCWGG 2 cut(s) 521, 568
AluBI AGCT 1 cut(s) 509
AluI AGCT 1 cut(s) 509
Alw26I GTCTC 2 cut(s) 709, 930
AlwI GGATC 4 cut(s) 107, 214, 449, 462
AlwNI CAGNNNCTG 1 cut(s) 278
ApeKI GCWGC 1 cut(s) 518
ApoI RAATTY 1 cut(s) 347
ArsI GACNNNNNNTTYG 2 cut(s) 665, 697
AseI ATTAAT 1 cut(s) 906
AsiGI ACCGGT 2 cut(s) 641, 678
Asp700I GAANNNNTTC 2 cut(s) 171, 965
Asp718I GGTACC 1 cut(s) 650
AspLEI GCGC 2 cut(s) 344, 493
AspS9I GGNCC 2 cut(s) 584, 681
AsuHPI GGTGA 1 cut(s) 412
AvaII GGWCC 2 cut(s) 584, 681
AxyI CCTNAGG 1 cut(s) 40
BamHI GGATCC 1 cut(s) 454
BanI GGYRCC 1 cut(s) 650
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 1 cut(s) 505
BccI CCATC 2 cut(s) 149, 477
BciT130I CCWGG 2 cut(s) 523, 570
BcoDI GTCTC 2 cut(s) 709, 930
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 2 cut(s) 152, 296
BfmI CTRYAG 1 cut(s) 306
BisI GCNGC 1 cut(s) 519
BlsI GCNGC 1 cut(s) 520
Bme1390I CCNGG 2 cut(s) 523, 570
Bme18I GGWCC 2 cut(s) 584, 681
BmgT120I GGNCC 2 cut(s) 584, 681
BmiI GGNNCC 3 cut(s) 37, 456, 652
BmrFI CCNGG 2 cut(s) 523, 570
BmsI GCATC 2 cut(s) 62, 263
BoxI GACNNNNGTC 1 cut(s) 231
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 1 cut(s) 956
BsaJI CCNNGG 2 cut(s) 569, 783
BsaWI WCCGGW 2 cut(s) 641, 678
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 919
Bse118I RCCGGY 2 cut(s) 641, 678
Bse21I CCTNAGG 1 cut(s) 40
BseBI CCWGG 2 cut(s) 523, 570
BseDI CCNNGG 2 cut(s) 569, 783
BseGI GGATG 3 cut(s) 236, 442, 703
BseLI CCNNNNNNNGG 1 cut(s) 919
BseMII CTCAG 2 cut(s) 54, 501
BseRI GAGGAG 1 cut(s) 527
BseXI GCAGC 1 cut(s) 505
Bsh1236I CGCG 1 cut(s) 344
BshNI GGYRCC 1 cut(s) 650
BshTI ACCGGT 2 cut(s) 641, 678
BsiSI CCGG 2 cut(s) 642, 679
BslI CCNNNNNNNGG 1 cut(s) 919
BsmAI GTCTC 2 cut(s) 709, 930
Bsp143I GATC 3 cut(s) 112, 206, 454
BspCNI CTCAG 2 cut(s) 53, 502
BspFNI CGCG 1 cut(s) 344
BspLI GGNNCC 3 cut(s) 37, 456, 652
BspMAI CTGCAG 1 cut(s) 310
BspPI GGATC 4 cut(s) 107, 214, 449, 462
BspT107I GGYRCC 1 cut(s) 650
BsrFI RCCGGY 2 cut(s) 641, 678
BssAI RCCGGY 2 cut(s) 641, 678
BssECI CCNNGG 2 cut(s) 569, 783
BssMI GATC 3 cut(s) 112, 206, 454
Bst2UI CCWGG 2 cut(s) 523, 570
Bst4CI ACNGT 5 cut(s) 94, 106, 220, 358, 616
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 3 cut(s) 40, 510, 932
BstF5I GGATG 3 cut(s) 236, 442, 703
BstFNI CGCG 1 cut(s) 344
BstHHI GCGC 2 cut(s) 344, 493
BstKTI GATC 3 cut(s) 115, 209, 457
BstMAI GTCTC 2 cut(s) 709, 930
BstMBI GATC 3 cut(s) 112, 206, 454
BstMWI GCNNNNNNNGC 2 cut(s) 515, 887
BstNI CCWGG 2 cut(s) 523, 570
BstPAI GACNNNNGTC 1 cut(s) 231
BstSCI CCNGG 2 cut(s) 521, 568
BstSFI CTRYAG 1 cut(s) 306
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 1 cut(s) 505
BstX2I RGATCY 2 cut(s) 206, 454
BstYI RGATCY 2 cut(s) 206, 454
Bsu36I CCTNAGG 1 cut(s) 40
BtsCI GGATG 3 cut(s) 236, 442, 703
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 4 cut(s) 102, 276, 315, 612
Cac8I GCNNGC 1 cut(s) 306
CaiI CAGNNNCTG 1 cut(s) 278
CfoI GCGC 2 cut(s) 344, 493
Cfr10I RCCGGY 2 cut(s) 641, 678
Cfr13I GGNCC 2 cut(s) 584, 681
Csp6I GTAC 2 cut(s) 645, 651
CspAI ACCGGT 2 cut(s) 641, 678
CviAII CATG 3 cut(s) 57, 339, 444
CviQI GTAC 2 cut(s) 645, 651
DdeI CTNAG 3 cut(s) 40, 510, 932
DpnI GATC 3 cut(s) 114, 208, 456
DpnII GATC 3 cut(s) 112, 206, 454
Eco47I GGWCC 2 cut(s) 584, 681
Eco57I CTGAAG 4 cut(s) 375, 720, 812, 974
Eco81I CCTNAGG 1 cut(s) 40
EcoRII CCWGG 2 cut(s) 521, 568
FaeI CATG 3 cut(s) 60, 342, 447
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FatI CATG 3 cut(s) 56, 338, 443
Fnu4HI GCNGC 1 cut(s) 519
FokI GGATG 3 cut(s) 243, 449, 710
Fsp4HI GCNGC 1 cut(s) 519
FspBI CTAG 2 cut(s) 152, 296
GlaI GCGC 2 cut(s) 343, 492
GluI GCNGC 1 cut(s) 519
HapII CCGG 2 cut(s) 642, 679
HhaI GCGC 2 cut(s) 344, 493
Hin1II CATG 3 cut(s) 60, 342, 447
Hin6I GCGC 2 cut(s) 342, 491
HinP1I GCGC 2 cut(s) 342, 491
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 4 cut(s) 122, 565, 625, 631
HpaII CCGG 2 cut(s) 642, 679
HphI GGTGA 1 cut(s) 412
Hpy166II GTNNAC 2 cut(s) 226, 587
Hpy188I TCNGA 7 cut(s) 417, 424, 454, 630, 784, 792, 859
Hpy188III TCNNGA 4 cut(s) 110, 361, 830, 973
Hpy8I GTNNAC 2 cut(s) 226, 587
HpyAV CCTTC 7 cut(s) 197, 307, 744, 836, 923, 949, 955
HpyCH4III ACNGT 5 cut(s) 94, 106, 220, 358, 616
HpyCH4V TGCA 2 cut(s) 308, 823
HpyF10VI GCNNNNNNNGC 2 cut(s) 515, 887
HpyF3I CTNAG 3 cut(s) 40, 510, 932
Hsp92II CATG 3 cut(s) 60, 342, 447
HspAI GCGC 2 cut(s) 342, 491
KpnI GGTACC 1 cut(s) 654
Kzo9I GATC 3 cut(s) 112, 206, 454
LmnI GCTCC 2 cut(s) 35, 158
Lsp1109I GCAGC 1 cut(s) 505
LweI GCATC 2 cut(s) 62, 263
MaeI CTAG 2 cut(s) 152, 296
MaeIII GTNAC 5 cut(s) 28, 44, 274, 556, 769
MalI GATC 3 cut(s) 114, 208, 456
MboI GATC 3 cut(s) 112, 206, 454
MboII GAAGA 5 cut(s) 212, 372, 484, 759, 805
MfeI CAATTG 1 cut(s) 942
MflI RGATCY 2 cut(s) 206, 454
MluCI AATT 6 cut(s) 347, 546, 673, 903, 907, 942
MmeI TCCRAC 1 cut(s) 900
MnlI CCTC 6 cut(s) 49, 423, 505, 508, 778, 980
MroXI GAANNNNTTC 2 cut(s) 171, 965
MseI TTAA 4 cut(s) 324, 374, 606, 906
MspI CCGG 2 cut(s) 642, 679
MspR9I CCNGG 2 cut(s) 523, 570
MunI CAATTG 1 cut(s) 942
MvaI CCWGG 2 cut(s) 523, 570
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 2 cut(s) 515, 887
NdeII GATC 3 cut(s) 112, 206, 454
NlaIII CATG 3 cut(s) 60, 342, 447
NlaIV GGNNCC 3 cut(s) 37, 456, 652
NmuCI GTSAC 1 cut(s) 274
PdmI GAANNNNTTC 2 cut(s) 171, 965
PfeI GAWTC 4 cut(s) 122, 565, 625, 631
PinAI ACCGGT 2 cut(s) 641, 678
PkrI GCNGC 1 cut(s) 520
PshAI GACNNNNGTC 1 cut(s) 231
PshBI ATTAAT 1 cut(s) 906
Psp6I CCWGG 2 cut(s) 521, 568
PspGI CCWGG 2 cut(s) 521, 568
PspN4I GGNNCC 3 cut(s) 37, 456, 652
PspPI GGNCC 2 cut(s) 584, 681
PstI CTGCAG 1 cut(s) 310
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 2 cut(s) 206, 454
RsaI GTAC 2 cut(s) 646, 652
RsaNI GTAC 2 cut(s) 645, 651
SaqAI TTAA 4 cut(s) 324, 374, 606, 906
SatI GCNGC 1 cut(s) 519
Sau3AI GATC 3 cut(s) 112, 206, 454
Sau96I GGNCC 2 cut(s) 584, 681
ScrFI CCNGG 2 cut(s) 523, 570
SfaNI GCATC 2 cut(s) 62, 263
SfcI CTRYAG 1 cut(s) 306
SinI GGWCC 2 cut(s) 584, 681
SmlI CTYRAG 1 cut(s) 971
SmoI CTYRAG 1 cut(s) 971
SpeI ACTAGT 1 cut(s) 295
Sse9I AATT 6 cut(s) 347, 546, 673, 903, 907, 942
SspMI CTAG 2 cut(s) 152, 296
StyD4I CCNGG 2 cut(s) 521, 568
TaaI ACNGT 5 cut(s) 94, 106, 220, 358, 616
TasI AATT 6 cut(s) 347, 546, 673, 903, 907, 942
TfiI GAWTC 4 cut(s) 122, 565, 625, 631
Tru1I TTAA 4 cut(s) 324, 374, 606, 906
Tru9I TTAA 4 cut(s) 324, 374, 606, 906
TscAI CASTG 4 cut(s) 109, 283, 315, 619
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 1 cut(s) 518
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 3 cut(s) 236, 453, 578
TspGWI ACGGA 2 cut(s) 243, 906
TspRI CASTG 4 cut(s) 109, 283, 315, 619
VpaK11BI GGWCC 2 cut(s) 584, 681
VspI ATTAAT 1 cut(s) 906
XapI RAATTY 1 cut(s) 347
XmnI GAANNNNTTC 2 cut(s) 171, 965
XspI CTAG 2 cut(s) 152, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.