pycom07g01630

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000709
Physical Location & Seq
Reverse (-)
1289562 .. 1291184
1623 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g01630.1

Sequence Viewer

Length: 966 bp
ATGAGTACCGGAGAATCAAAGACTGTATGTGTAACAGGAGCTTCCGGTTACATAGCATCATGGCTGGTGAAGCTCTTACTGCAAAAGGGTTATACCGTCAAGGCCACTGTTCGCGATCCAAATGATGCAAAGAAAACAGAACACTTGCTCTCATTAGATGGAGCAAAGGAGAGGCTTCATTTGTTCAAAGCGGATTTATTAGAAGAAGGATCATTTGACGCTGTAATTGATGGATGTGTAGGCGTTTTTCATACAGCATCGCCTGCACAATTTTCAGTCACTGACCCACAGGTAGAAATAATTGAGCCTGCAGTGAAGGGAACACTTAATGTTCTAAATTCATGCGCAAAATTCCCCGCTGTCAAGAGGGTGGTTATAACATCCTCTCTGGCTTCAGTAGGGTTGAGTGGAAAACCTCTGACCTCTGATGTGGTTATGGATGAAACATGGTACTCGGATCCACTTTTTTGCGAGACGACCAAGCAATGGTATCCTCTCTCAAAAACTTTAGCTGAGGAGGCCGCCTGGAAATTTGCCAAAGGAAATGGTATTGACTTGGTAACAATACATCCAGGGAATGCGATTGGTCCACCCTTACAGCCAAACCTTAATCTCTCCGTCGAGTTTCTTCTGAATTTAATGAGTGGTATCGAAACACCTTTTATAAACTATACATTTGTGGATGTTAGAGATGTTGCCTTTGCTCATATTCAAGCATTTGAAGTTCCTTCAGCTAGTGGCAGATATTGTTTAGTTGCACAAGTTGCTGATGGTCCCGATACTCTAAAGATTTTACGAGAGCTTTACCCCACATTGAGCCTTTGTGAGCCTGGCAATCCTTCTGGCTCCAAGTTTCAAGTGTCCAAGGAGAAGGCAAAATGTTTGGGAATCACTTTCCTTCCTCTGGAAACAAGTCTCAAGGACACTGTTGAAAGCCTCATGGAGAAGGGCTTCCTCAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

322

Amino Acids

34.81

Weight (kDa)

5.49

Isoelectric Point (pI)

26.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 101 8e-10 NmrA-like family
Epimerase PF01370 9 - 245 5.7e-23 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 239 1.8e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 197 2.1e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 206 1.9e-10 Male sterility protein
NAD_binding_10 PF13460 13 - 131 2.8e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 377, 665
Acc16I TGCGCA 1 cut(s) 346
AccB7I CCANNNNNTGG 1 cut(s) 486
AccII CGCG 1 cut(s) 114
AciI CCGC 3 cut(s) 191, 357, 522
AclWI GGATC 4 cut(s) 110, 217, 452, 465
AcsI RAATTY 4 cut(s) 337, 350, 530, 634
AcuI CTGAAG 2 cut(s) 378, 714
AfaI GTAC 2 cut(s) 7, 452
AfiI CCNNNNNNNGG 2 cut(s) 486, 904
AgsI TTSAA 5 cut(s) 187, 713, 722, 857, 932
AjnI CCWGG 3 cut(s) 524, 571, 829
AluBI AGCT 5 cut(s) 41, 73, 512, 734, 802
AluI AGCT 5 cut(s) 41, 73, 512, 734, 802
Alw26I GTCTC 2 cut(s) 467, 920
AlwI GGATC 4 cut(s) 110, 217, 452, 465
AlwNI CAGNNNCTG 1 cut(s) 281
AoxI GGCC 2 cut(s) 102, 519
ApoI RAATTY 4 cut(s) 337, 350, 530, 634
Asp700I GAANNNNTTC 1 cut(s) 950
AspLEI GCGC 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 587, 773
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 2 cut(s) 587, 773
BamHI GGATCC 1 cut(s) 457
BbvCI CCTCAGC 1 cut(s) 513
BccI CCATC 3 cut(s) 152, 224, 764
BciT130I CCWGG 3 cut(s) 526, 573, 831
BciVI GTATCC 1 cut(s) 501
BcoDI GTCTC 2 cut(s) 467, 920
BfaI CTAG 1 cut(s) 735
BfmI CTRYAG 1 cut(s) 309
BfuI GTATCC 1 cut(s) 501
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
Bme1390I CCNGG 3 cut(s) 526, 573, 831
Bme18I GGWCC 2 cut(s) 587, 773
BmgT120I GGNCC 2 cut(s) 587, 773
BmiI GGNNCC 3 cut(s) 459, 775, 847
BmrFI CCNGG 3 cut(s) 526, 573, 831
BmsI GCATC 3 cut(s) 65, 115, 266
Bpu10I CCTNAGC 1 cut(s) 513
BpuEI CTTGAG 2 cut(s) 902, 941
BsaJI CCNNGG 2 cut(s) 572, 864
BsaWI WCCGGW 2 cut(s) 8, 44
Bsc4I CCNNNNNNNGG 2 cut(s) 486, 904
Bse3DI GCAATG 1 cut(s) 491
BseBI CCWGG 3 cut(s) 526, 573, 831
BseDI CCNNGG 2 cut(s) 572, 864
BseGI GGATG 5 cut(s) 239, 380, 445, 568, 688
BseLI CCNNNNNNNGG 2 cut(s) 486, 904
BseMI GCAATG 1 cut(s) 491
BseMII CTCAG 1 cut(s) 504
BseRI GAGGAG 1 cut(s) 530
BsgI GTGCAG 1 cut(s) 249
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 2 cut(s) 104, 521
BsiSI CCGG 2 cut(s) 9, 45
BslFI GGGAC 1 cut(s) 759
BslI CCNNNNNNNGG 2 cut(s) 486, 904
BsmAI GTCTC 2 cut(s) 467, 920
BsmBI CGTCTC 1 cut(s) 467
BsmFI GGGAC 1 cut(s) 759
BsmI GAATGC 1 cut(s) 583
BsnI GGCC 2 cut(s) 104, 521
Bsp143I GATC 3 cut(s) 115, 209, 457
Bsp68I TCGCGA 1 cut(s) 114
BspACI CCGC 3 cut(s) 191, 357, 522
BspANI GGCC 2 cut(s) 104, 521
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 3 cut(s) 459, 775, 847
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 4 cut(s) 110, 217, 452, 465
BsrDI GCAATG 1 cut(s) 491
BssECI CCNNGG 2 cut(s) 572, 864
BssMI GATC 3 cut(s) 115, 209, 457
BssT1I CCWWGG 1 cut(s) 864
Bst2UI CCWGG 3 cut(s) 526, 573, 831
Bst4CI ACNGT 4 cut(s) 25, 97, 109, 928
BstAPI GCANNNNNTGC 2 cut(s) 263, 764
BstC8I GCNNGC 2 cut(s) 264, 309
BstDEI CTNAG 1 cut(s) 513
BstF5I GGATG 5 cut(s) 239, 380, 445, 568, 688
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 3 cut(s) 118, 212, 460
BstMAI GTCTC 2 cut(s) 467, 920
BstMBI GATC 3 cut(s) 115, 209, 457
BstMWI GCNNNNNNNGC 5 cut(s) 70, 79, 263, 518, 764
BstNI CCWGG 3 cut(s) 526, 573, 831
BstSCI CCNGG 3 cut(s) 524, 571, 829
BstSFI CTRYAG 1 cut(s) 309
BstUI CGCG 1 cut(s) 114
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
BsuI GTATCC 1 cut(s) 501
BsuRI GGCC 2 cut(s) 104, 521
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 5 cut(s) 239, 380, 445, 568, 688
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 4 cut(s) 105, 279, 318, 924
BtuMI TCGCGA 1 cut(s) 114
Cac8I GCNNGC 2 cut(s) 264, 309
CaiI CAGNNNCTG 1 cut(s) 281
CfoI GCGC 1 cut(s) 347
Cfr13I GGNCC 2 cut(s) 587, 773
CseI GACGC 1 cut(s) 227
Csp6I GTAC 2 cut(s) 6, 451
CviAII CATG 4 cut(s) 60, 342, 447, 940
CviQI GTAC 2 cut(s) 6, 451
DdeI CTNAG 1 cut(s) 513
DpnI GATC 3 cut(s) 117, 211, 459
DpnII GATC 3 cut(s) 115, 209, 457
Eco130I CCWWGG 1 cut(s) 864
Eco47I GGWCC 2 cut(s) 587, 773
Eco57I CTGAAG 2 cut(s) 378, 714
EcoRII CCWGG 3 cut(s) 524, 571, 829
EcoT14I CCWWGG 1 cut(s) 864
ErhI CCWWGG 1 cut(s) 864
Esp3I CGTCTC 1 cut(s) 467
FaeI CATG 4 cut(s) 63, 345, 450, 943
FaqI GGGAC 1 cut(s) 759
FatI CATG 4 cut(s) 59, 341, 446, 939
FauI CCCGC 1 cut(s) 364
Fnu4HI GCNGC 1 cut(s) 522
FokI GGATG 5 cut(s) 246, 367, 452, 555, 695
Fsp4HI GCNGC 1 cut(s) 522
FspBI CTAG 1 cut(s) 735
FspI TGCGCA 1 cut(s) 346
GlaI GCGC 1 cut(s) 346
GluI GCNGC 1 cut(s) 522
HaeIII GGCC 2 cut(s) 104, 521
HapII CCGG 2 cut(s) 9, 45
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 347
Hin1II CATG 4 cut(s) 63, 345, 450, 943
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HinfI GANTC 2 cut(s) 14, 888
HpaII CCGG 2 cut(s) 9, 45
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 1 cut(s) 590
Hpy188I TCNGA 4 cut(s) 420, 427, 457, 633
Hpy188III TCNNGA 4 cut(s) 113, 364, 776, 905
Hpy8I GTNNAC 1 cut(s) 590
Hpy99I CGWCG 1 cut(s) 623
HpyAV CCTTC 7 cut(s) 200, 310, 738, 849, 865, 908, 940
HpyCH4III ACNGT 4 cut(s) 25, 97, 109, 928
HpyCH4V TGCA 5 cut(s) 82, 128, 266, 311, 758
HpyF10VI GCNNNNNNNGC 5 cut(s) 70, 79, 263, 518, 764
HpyF3I CTNAG 1 cut(s) 513
Hsp92II CATG 4 cut(s) 63, 345, 450, 943
HspAI GCGC 1 cut(s) 345
Kzo9I GATC 3 cut(s) 115, 209, 457
LmnI GCTCC 3 cut(s) 38, 161, 851
LweI GCATC 3 cut(s) 65, 115, 266
MaeI CTAG 1 cut(s) 735
MaeIII GTNAC 4 cut(s) 31, 47, 277, 559
MalI GATC 3 cut(s) 117, 211, 459
MboI GATC 3 cut(s) 115, 209, 457
MboII GAAGA 2 cut(s) 215, 620
MflI RGATCY 1 cut(s) 457
MluCI AATT 7 cut(s) 225, 269, 300, 337, 350, 530, 634
MroXI GAANNNNTTC 1 cut(s) 950
MseI TTAA 3 cut(s) 327, 609, 638
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 2 cut(s) 9, 45
MspR9I CCNGG 3 cut(s) 526, 573, 831
Mva1269I GAATGC 1 cut(s) 583
MvaI CCWGG 3 cut(s) 526, 573, 831
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 5 cut(s) 70, 79, 263, 518, 764
NdeII GATC 3 cut(s) 115, 209, 457
NlaIII CATG 4 cut(s) 63, 345, 450, 943
NlaIV GGNNCC 3 cut(s) 459, 775, 847
NmuCI GTSAC 1 cut(s) 277
NruI TCGCGA 1 cut(s) 114
NsbI TGCGCA 1 cut(s) 346
PctI GAATGC 1 cut(s) 583
PdmI GAANNNNTTC 1 cut(s) 950
PfeI GAWTC 2 cut(s) 14, 888
PflMI CCANNNNNTGG 1 cut(s) 486
PkrI GCNGC 1 cut(s) 523
PsiI TTATAA 2 cut(s) 377, 665
Psp6I CCWGG 3 cut(s) 524, 571, 829
PspGI CCWGG 3 cut(s) 524, 571, 829
PspN4I GGNNCC 3 cut(s) 459, 775, 847
PspPI GGNCC 2 cut(s) 587, 773
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 1 cut(s) 457
RruI TCGCGA 1 cut(s) 114
RsaI GTAC 2 cut(s) 7, 452
RsaNI GTAC 2 cut(s) 6, 451
SaqAI TTAA 3 cut(s) 327, 609, 638
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 3 cut(s) 115, 209, 457
Sau96I GGNCC 2 cut(s) 587, 773
ScrFI CCNGG 3 cut(s) 526, 573, 831
SfaNI GCATC 3 cut(s) 65, 115, 266
SfcI CTRYAG 1 cut(s) 309
SinI GGWCC 2 cut(s) 587, 773
SmlI CTYRAG 2 cut(s) 917, 956
SmoI CTYRAG 2 cut(s) 917, 956
Sse9I AATT 7 cut(s) 225, 269, 300, 337, 350, 530, 634
SsiI CCGC 3 cut(s) 191, 357, 522
SspMI CTAG 1 cut(s) 735
StyD4I CCNGG 3 cut(s) 524, 571, 829
StyI CCWWGG 1 cut(s) 864
TaaI ACNGT 4 cut(s) 25, 97, 109, 928
TaqI TCGA 2 cut(s) 621, 651
TasI AATT 7 cut(s) 225, 269, 300, 337, 350, 530, 634
TauI GCSGC 1 cut(s) 524
TfiI GAWTC 2 cut(s) 14, 888
Tru1I TTAA 3 cut(s) 327, 609, 638
Tru9I TTAA 3 cut(s) 327, 609, 638
TscAI CASTG 4 cut(s) 112, 286, 318, 931
TseFI GTSAC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 4 cut(s) 167, 239, 330, 456
TspGWI ACGGA 1 cut(s) 607
TspRI CASTG 4 cut(s) 112, 286, 318, 931
Van91I CCANNNNNTGG 1 cut(s) 486
VpaK11BI GGWCC 2 cut(s) 587, 773
XapI RAATTY 4 cut(s) 337, 350, 530, 634
XmnI GAANNNNTTC 1 cut(s) 950
XspI CTAG 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.