RchiOBHm_Chr1g0317581

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000709
Physical Location & Seq
Reverse (-)
5102204 .. 5106307
4104 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54788

Sequence Viewer

Length: 810 bp
ATGAGTGGAGCAAGGAAAGTTGCTGTGTGTGTAACTGGAGCGTCTGGTTATATTGCATCATGGCTGGTCAAGCTTTTGTTAGGACGAGGTTATATTGTCAAAGCCACTGTCCGGGACCCAAACGATCCGAAGAAAACAGAACACTTGCTTTCACTCGATGGAGCAAAAGAAAGGCTTCATTTGTTCAAAGCAGATTTGCTAGATGAAGGATCTTTTGACCCTGTGGTTGATGGATGTGACGGTGTTTTCCATACAGCATCTCCAGTACTATTTTCATCGATCAGTGACCCACAGGCAGAACTAATTGACCCTGCTGTGAAGGGAACACTAAATGTTCTGAAATCATGCAAAAAATTTCCAACCGTCAAGCGAGTGGTTTTGACATCTTCTATGGCTTCAGTAATGATGAATGGAAAACCTCTGACCCCTGATGTTGTGGTGGATGAAACATGGTATTCAGATCCACTTATTGCTGAGAAGCACAAGCTATGGTATCTTCTTTCAAAGACTTTAGCTGAGGAGGCTGCTTGGAATTTTGCTGAAGGAAATGGGATTGACTTGGTTTCAATAAATCCGGGGTTTGTGATAGGACCACTCTTACAGCCGACAATTAATTTGACCGTGGAGATGATTCAGAATCTCATAACTGGGCAGTGTGAGGATGACAATCCTCTTTTGCTTCCTTCTGACCCACAGTTTCAAGTATCCAAGGAGCAGGCAAAAGGTCTGGGAATTGGTTTTTATCCATTTGAAGCAACTCTGAAGGACACTATTGAAAGCCTGAAGGAGAAGGGCTTTCTCAACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

269

Amino Acids

29.29

Weight (kDa)

5.19

Isoelectric Point (pI)

23.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 8 - 106 1.4e-08 NmrA-like family
Epimerase PF01370 9 - 213 3.5e-21 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 211 2.4e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 191 2e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 201 2.2e-11 Male sterility protein
NAD_binding_10 PF13460 13 - 134 3e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 119, 217, 455
AcsI RAATTY 2 cut(s) 353, 532
AcuI CTGAAG 4 cut(s) 381, 561, 782, 803
AfaI GTAC 1 cut(s) 267
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 6 cut(s) 187, 504, 567, 701, 752, 776
AluBI AGCT 3 cut(s) 73, 487, 515
AluI AGCT 3 cut(s) 73, 487, 515
AlwI GGATC 3 cut(s) 119, 217, 455
ApeKI GCWGC 1 cut(s) 524
ApoI RAATTY 2 cut(s) 353, 532
ArsI GACNNNNNNTTYG 4 cut(s) 93, 125, 598, 630
AseI ATTAAT 1 cut(s) 612
Asp700I GAANNNNTTC 1 cut(s) 174
AspS9I GGNCC 2 cut(s) 115, 590
AsuC2I CCSGG 2 cut(s) 113, 576
AvaII GGWCC 2 cut(s) 115, 590
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 1 cut(s) 511
BccI CCATC 2 cut(s) 152, 224
BciVI GTATCC 1 cut(s) 715
BcnI CCSGG 2 cut(s) 113, 576
BfaI CTAG 1 cut(s) 200
BfuI GTATCC 1 cut(s) 715
BisI GCNGC 1 cut(s) 525
BlsI GCNGC 1 cut(s) 526
BmcAI AGTACT 1 cut(s) 267
Bme1390I CCNGG 2 cut(s) 113, 576
Bme18I GGWCC 2 cut(s) 115, 590
BmgT120I GGNCC 2 cut(s) 115, 590
BmiI GGNNCC 2 cut(s) 116, 117
BmrFI CCNGG 2 cut(s) 113, 576
BmrI ACTGGG 1 cut(s) 657
BmsI GCATC 2 cut(s) 65, 266
BmuI ACTGGG 1 cut(s) 657
BpmI CTGGAG 2 cut(s) 57, 246
Bpu10I CCTNAGC 1 cut(s) 516
BpuMI CCSGG 2 cut(s) 113, 576
Bsa29I ATCGAT 1 cut(s) 278
BsaBI GATNNNNATC 1 cut(s) 666
BsaJI CCNNGG 3 cut(s) 575, 621, 708
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 3 cut(s) 40, 263, 652
Bse8I GATNNNNATC 1 cut(s) 666
BseCI ATCGAT 1 cut(s) 278
BseDI CCNNGG 3 cut(s) 575, 621, 708
BseGI GGATG 3 cut(s) 239, 448, 667
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 2 cut(s) 465, 507
BseNI ACTGG 3 cut(s) 40, 263, 652
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 1 cut(s) 511
BshVI ATCGAT 1 cut(s) 278
BsiSI CCGG 2 cut(s) 112, 575
BslFI GGGAC 1 cut(s) 128
BslI CCNNNNNNNGG 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 128
Bsp143I GATC 4 cut(s) 124, 209, 279, 460
BspCNI CTCAG 2 cut(s) 466, 508
BspDI ATCGAT 1 cut(s) 278
BspLI GGNNCC 2 cut(s) 116, 117
BspPI GGATC 3 cut(s) 119, 217, 455
BsrI ACTGG 3 cut(s) 40, 263, 652
BssECI CCNNGG 3 cut(s) 575, 621, 708
BssMI GATC 4 cut(s) 124, 209, 279, 460
BssT1I CCWWGG 1 cut(s) 708
Bst4CI ACNGT 5 cut(s) 109, 242, 364, 622, 696
BstC8I GCNNGC 1 cut(s) 717
BstDEI CTNAG 2 cut(s) 474, 516
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 3 cut(s) 239, 448, 667
BstKTI GATC 4 cut(s) 127, 212, 282, 463
BstMBI GATC 4 cut(s) 124, 209, 279, 460
BstMWI GCNNNNNNNGC 2 cut(s) 70, 521
BstSCI CCNGG 2 cut(s) 111, 574
BstV1I GCAGC 1 cut(s) 511
BstX2I RGATCY 2 cut(s) 209, 460
BstYI RGATCY 2 cut(s) 209, 460
Bsu15I ATCGAT 1 cut(s) 278
BsuI GTATCC 1 cut(s) 715
BsuTUI ATCGAT 1 cut(s) 278
BtgI CCRYGG 1 cut(s) 621
BtsCI GGATG 3 cut(s) 239, 448, 667
BtsI GCAGTG 1 cut(s) 659
BtsIMutI CAGTG 3 cut(s) 105, 289, 659
Cac8I GCNNGC 1 cut(s) 717
Cfr13I GGNCC 2 cut(s) 115, 590
ClaI ATCGAT 1 cut(s) 278
CseI GACGC 1 cut(s) 30
Csp6I GTAC 1 cut(s) 266
CviAII CATG 3 cut(s) 60, 345, 450
CviQI GTAC 1 cut(s) 266
DdeI CTNAG 2 cut(s) 474, 516
DpnI GATC 4 cut(s) 126, 211, 281, 462
DpnII GATC 4 cut(s) 124, 209, 279, 460
Eco130I CCWWGG 1 cut(s) 708
Eco47I GGWCC 2 cut(s) 115, 590
Eco57I CTGAAG 4 cut(s) 381, 561, 782, 803
EcoO109I RGGNCCY 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 708
ErhI CCWWGG 1 cut(s) 708
FaeI CATG 3 cut(s) 63, 348, 453
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 1 cut(s) 128
FatI CATG 3 cut(s) 59, 344, 449
FauNDI CATATG 1 cut(s) 806
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 3 cut(s) 246, 455, 674
Fsp4HI GCNGC 1 cut(s) 525
FspBI CTAG 1 cut(s) 200
GluI GCNGC 1 cut(s) 525
GsuI CTGGAG 2 cut(s) 57, 246
HapII CCGG 2 cut(s) 112, 575
HgaI GACGC 1 cut(s) 30
Hin1II CATG 3 cut(s) 63, 348, 453
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 2 cut(s) 631, 637
HpaII CCGG 2 cut(s) 112, 575
Hpy188I TCNGA 7 cut(s) 129, 339, 423, 460, 636, 688, 762
HpyAV CCTTC 7 cut(s) 200, 313, 536, 693, 757, 778, 784
HpyCH4III ACNGT 5 cut(s) 109, 242, 364, 622, 696
HpyCH4V TGCA 2 cut(s) 56, 348
HpyF10VI GCNNNNNNNGC 2 cut(s) 70, 521
HpyF3I CTNAG 2 cut(s) 474, 516
Hsp92II CATG 3 cut(s) 63, 348, 453
KflI GGGWCCC 1 cut(s) 115
Kzo9I GATC 4 cut(s) 124, 209, 279, 460
LmnI GCTCC 4 cut(s) 8, 38, 161, 712
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 2 cut(s) 65, 266
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 3 cut(s) 31, 236, 284
MalI GATC 4 cut(s) 126, 211, 281, 462
MboI GATC 4 cut(s) 124, 209, 279, 460
MboII GAAGA 3 cut(s) 142, 378, 488
MflI RGATCY 2 cut(s) 209, 460
MluCI AATT 6 cut(s) 303, 353, 532, 609, 613, 732
MmeI TCCRAC 1 cut(s) 383
MnlI CCTC 6 cut(s) 80, 429, 511, 514, 652, 681
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 1 cut(s) 612
MspI CCGG 2 cut(s) 112, 575
MspR9I CCNGG 2 cut(s) 113, 576
MwoI GCNNNNNNNGC 2 cut(s) 70, 521
NciI CCSGG 2 cut(s) 113, 576
NdeI CATATG 1 cut(s) 806
NdeII GATC 4 cut(s) 124, 209, 279, 460
NlaIII CATG 3 cut(s) 63, 348, 453
NlaIV GGNNCC 2 cut(s) 116, 117
NmuCI GTSAC 2 cut(s) 236, 284
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 2 cut(s) 631, 637
PfoI TCCNGGA 1 cut(s) 111
PkrI GCNGC 1 cut(s) 526
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 612
Psp5II RGGWCCY 1 cut(s) 115
PspN4I GGNNCC 2 cut(s) 116, 117
PspPI GGNCC 2 cut(s) 115, 590
PspPPI RGGWCCY 1 cut(s) 115
PsuI RGATCY 2 cut(s) 209, 460
RsaI GTAC 1 cut(s) 267
RsaNI GTAC 1 cut(s) 266
SaqAI TTAA 1 cut(s) 612
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 4 cut(s) 124, 209, 279, 460
Sau96I GGNCC 2 cut(s) 115, 590
ScaI AGTACT 1 cut(s) 267
ScrFI CCNGG 2 cut(s) 113, 576
SetI ASST 6 cut(s) 75, 91, 421, 489, 517, 727
SfaNI GCATC 2 cut(s) 65, 266
SinI GGWCC 2 cut(s) 115, 590
Sse9I AATT 6 cut(s) 303, 353, 532, 609, 613, 732
SspMI CTAG 1 cut(s) 200
StyD4I CCNGG 2 cut(s) 111, 574
StyI CCWWGG 1 cut(s) 708
TaaI ACNGT 5 cut(s) 109, 242, 364, 622, 696
TaqI TCGA 2 cut(s) 156, 278
TasI AATT 6 cut(s) 303, 353, 532, 609, 613, 732
TatI WGTACW 1 cut(s) 265
TfiI GAWTC 2 cut(s) 631, 637
Tru1I TTAA 1 cut(s) 612
Tru9I TTAA 1 cut(s) 612
TscAI CASTG 3 cut(s) 112, 289, 659
TseFI GTSAC 2 cut(s) 236, 284
TseI GCWGC 1 cut(s) 524
Tsp45I GTSAC 2 cut(s) 236, 284
TspDTI ATGAA 5 cut(s) 167, 219, 264, 422, 459
TspRI CASTG 3 cut(s) 112, 289, 659
VpaK11BI GGWCC 2 cut(s) 115, 590
VspI ATTAAT 1 cut(s) 612
XapI RAATTY 2 cut(s) 353, 532
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 1 cut(s) 200
ZrmI AGTACT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.