pycom02g25560

reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000709
Physical Location & Seq
Forward (+)
23425744 .. 23426902
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25560.2

Sequence Viewer

Length: 669 bp
ATGAGTACTGGAGAAACAAAGACTGTGTGTGTGACAGGAGCATCTGGTTACATAGCATCATGGCTCGTGAAGCTCTTACTACAGAAGGGTTATACTGTTAAAGGCACTGTTCGTGACCCAAATGATCCAAAGAAAACAGAACACTTACTCTCGCTAGATGGAGCAAAAGAAAGGCTTCATTTGTTGAAAGCGGATTTGTTAGAAGAAGGGGCTTTCGATGATGCAGTCGATGGATGTGTTGGTGTTTTTCATACAGCATCGCCTGCACAATTCTCAGCCACTGACCCACAGGCAGAAATAGTGGAGCCTGCAGTGAGAGGAACACTCAATGTTCTTAAATCATGCGCGAAATTCCCCGCTGTCAAGAGAGTGGTTTTAACATCATCCATGGCTTCAGTAACGGGGACCGGAACCCCTCTGACATCTGATGTGGTTTTGGATGAAACATGGTACTCAGATTCACTTCTTTGTGAGAAGAACAAGGAATGGTATCCTCTCTCAAAAACTTTAGCTGAGGAGGCTGCCTGGAAATTTGCTGAAGGAAATGGGATTGACTTGGTGTCGATGAATCCAGGGATTGCGATTGGTCCACTCTTACAGCCAACTCTTAATCTTTCTGTCGAGATATTTCGTAATCTCATAAGTGGTACGTTTCACCCCTTGTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

223

Amino Acids

23.84

Weight (kDa)

5.25

Isoelectric Point (pI)

21.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 347
AciI CCGC 2 cut(s) 191, 357
AclWI GGATC 1 cut(s) 119
AcsI RAATTY 2 cut(s) 350, 530
AcuI CTGAAG 2 cut(s) 378, 558
AfaI GTAC 3 cut(s) 7, 452, 649
AgsI TTSAA 1 cut(s) 187
AhdI GACNNNNNGTC 1 cut(s) 559
AjnI CCWGG 2 cut(s) 524, 571
AluBI AGCT 2 cut(s) 73, 512
AluI AGCT 2 cut(s) 73, 512
AlwI GGATC 1 cut(s) 119
AlwNI CAGNNNCTG 1 cut(s) 281
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 2 cut(s) 350, 530
Asp700I GAANNNNTTC 1 cut(s) 174
AspLEI GCGC 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 405, 587
AsuHPI GGTGA 1 cut(s) 647
AvaII GGWCC 2 cut(s) 405, 587
BauI CACGAG 1 cut(s) 65
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 1 cut(s) 508
BccI CCATC 2 cut(s) 152, 224
BciT130I CCWGG 2 cut(s) 526, 573
BciVI GTATCC 1 cut(s) 501
BfaI CTAG 1 cut(s) 155
BfmI CTRYAG 2 cut(s) 80, 309
BfuI GTATCC 1 cut(s) 501
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
BmcAI AGTACT 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 526, 573
Bme18I GGWCC 2 cut(s) 405, 587
BmeRI GACNNNNNGTC 1 cut(s) 559
BmgT120I GGNCC 2 cut(s) 405, 587
BmiI GGNNCC 3 cut(s) 306, 406, 412
BmrFI CCNGG 2 cut(s) 526, 573
BmsI GCATC 4 cut(s) 50, 65, 211, 266
BplI GAGNNNNNCTC 2 cut(s) 309, 341
BpmI CTGGAG 1 cut(s) 30
Bpu10I CCTNAGC 1 cut(s) 513
BsaJI CCNNGG 2 cut(s) 387, 572
BsaWI WCCGGW 1 cut(s) 407
Bse1I ACTGG 1 cut(s) 13
BseBI CCWGG 2 cut(s) 526, 573
BseDI CCNNGG 2 cut(s) 387, 572
BseGI GGATG 3 cut(s) 239, 383, 445
BseMII CTCAG 3 cut(s) 288, 468, 504
BseNI ACTGG 1 cut(s) 13
BseRI GAGGAG 1 cut(s) 530
BseXI GCAGC 1 cut(s) 508
BsgI GTGCAG 1 cut(s) 249
Bsh1236I CGCG 1 cut(s) 347
BsiSI CCGG 1 cut(s) 408
BslFI GGGAC 1 cut(s) 418
BsmFI GGGAC 1 cut(s) 418
Bsp143I GATC 1 cut(s) 124
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 2 cut(s) 191, 357
BspCNI CTCAG 3 cut(s) 287, 467, 505
BspFNI CGCG 1 cut(s) 347
BspLI GGNNCC 3 cut(s) 306, 406, 412
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 1 cut(s) 119
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 2 cut(s) 387, 572
BssMI GATC 1 cut(s) 124
BssSI CACGAG 1 cut(s) 65
BssT1I CCWWGG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 65
Bst2UI CCWGG 2 cut(s) 526, 573
Bst4CI ACNGT 3 cut(s) 25, 97, 109
BstAPI GCANNNNNTGC 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 264, 309
BstDEI CTNAG 3 cut(s) 274, 454, 513
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 3 cut(s) 239, 383, 445
BstFNI CGCG 1 cut(s) 347
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 1 cut(s) 127
BstMBI GATC 1 cut(s) 124
BstMWI GCNNNNNNNGC 3 cut(s) 70, 263, 518
BstNI CCWGG 2 cut(s) 526, 573
BstSCI CCNGG 2 cut(s) 524, 571
BstSFI CTRYAG 2 cut(s) 80, 309
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 1 cut(s) 508
BsuI GTATCC 1 cut(s) 501
BtgI CCRYGG 1 cut(s) 387
BtgZI GCGATG 1 cut(s) 243
BtsCI GGATG 3 cut(s) 239, 383, 445
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 3 cut(s) 105, 279, 318
Cac8I GCNNGC 2 cut(s) 264, 309
CaiI CAGNNNCTG 1 cut(s) 281
CfoI GCGC 1 cut(s) 347
Cfr13I GGNCC 2 cut(s) 405, 587
Csp6I GTAC 3 cut(s) 6, 451, 648
CviAII CATG 4 cut(s) 60, 342, 388, 447
CviQI GTAC 3 cut(s) 6, 451, 648
DdeI CTNAG 3 cut(s) 274, 454, 513
DpnI GATC 1 cut(s) 126
DpnII GATC 1 cut(s) 124
DriI GACNNNNNGTC 1 cut(s) 559
Eam1105I GACNNNNNGTC 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 387
Eco47I GGWCC 2 cut(s) 405, 587
Eco57I CTGAAG 2 cut(s) 378, 558
EcoRII CCWGG 2 cut(s) 524, 571
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 4 cut(s) 63, 345, 391, 450
FaiI YATR 8 cut(s) 53, 61, 93, 252, 343, 389, 448, 641
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 1 cut(s) 418
FatI CATG 4 cut(s) 59, 341, 387, 446
FauI CCCGC 1 cut(s) 364
Fnu4HI GCNGC 1 cut(s) 522
FokI GGATG 3 cut(s) 246, 370, 452
Fsp4HI GCNGC 1 cut(s) 522
FspBI CTAG 1 cut(s) 155
GlaI GCGC 1 cut(s) 346
GluI GCNGC 1 cut(s) 522
GsuI CTGGAG 1 cut(s) 30
HapII CCGG 1 cut(s) 408
HhaI GCGC 1 cut(s) 347
Hin1II CATG 4 cut(s) 63, 345, 391, 450
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HinfI GANTC 2 cut(s) 458, 568
HpaII CCGG 1 cut(s) 408
HphI GGTGA 1 cut(s) 647
Hpy166II GTNNAC 1 cut(s) 590
Hpy188I TCNGA 3 cut(s) 420, 427, 457
Hpy188III TCNNGA 4 cut(s) 67, 113, 364, 622
Hpy8I GTNNAC 1 cut(s) 590
HpyAV CCTTC 3 cut(s) 79, 200, 533
HpyCH4III ACNGT 3 cut(s) 25, 97, 109
HpyCH4IV ACGT 1 cut(s) 650
HpyCH4V TGCA 3 cut(s) 224, 266, 311
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 263, 518
HpyF3I CTNAG 3 cut(s) 274, 454, 513
HpySE526I ACGT 1 cut(s) 650
Hsp92II CATG 4 cut(s) 63, 345, 391, 450
HspAI GCGC 1 cut(s) 345
Kzo9I GATC 1 cut(s) 124
LmnI GCTCC 3 cut(s) 38, 161, 304
Lsp1109I GCAGC 1 cut(s) 508
LweI GCATC 4 cut(s) 50, 65, 211, 266
MaeI CTAG 1 cut(s) 155
MaeII ACGT 1 cut(s) 650
MaeIII GTNAC 4 cut(s) 31, 47, 113, 397
MalI GATC 1 cut(s) 126
MboI GATC 1 cut(s) 124
MboII GAAGA 2 cut(s) 215, 487
MluCI AATT 3 cut(s) 269, 350, 530
MnlI CCTC 5 cut(s) 311, 426, 504, 508, 511
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 4 cut(s) 99, 336, 377, 609
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 1 cut(s) 408
MspR9I CCNGG 2 cut(s) 526, 573
MvaI CCWGG 2 cut(s) 526, 573
MvnI CGCG 1 cut(s) 347
MwoI GCNNNNNNNGC 3 cut(s) 70, 263, 518
NcoI CCATGG 1 cut(s) 387
NdeII GATC 1 cut(s) 124
NlaIII CATG 4 cut(s) 63, 345, 391, 450
NlaIV GGNNCC 3 cut(s) 306, 406, 412
NmuCI GTSAC 2 cut(s) 31, 113
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 2 cut(s) 458, 568
PkrI GCNGC 1 cut(s) 523
Psp6I CCWGG 2 cut(s) 524, 571
PspGI CCWGG 2 cut(s) 524, 571
PspN4I GGNNCC 3 cut(s) 306, 406, 412
PspPI GGNCC 2 cut(s) 405, 587
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 281
RsaI GTAC 3 cut(s) 7, 452, 649
RsaNI GTAC 3 cut(s) 6, 451, 648
SaqAI TTAA 4 cut(s) 99, 336, 377, 609
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 1 cut(s) 124
Sau96I GGNCC 2 cut(s) 405, 587
ScaI AGTACT 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 526, 573
SetI ASST 3 cut(s) 75, 514, 653
SfaNI GCATC 4 cut(s) 50, 65, 211, 266
SfcI CTRYAG 2 cut(s) 80, 309
SinI GGWCC 2 cut(s) 405, 587
Sse9I AATT 3 cut(s) 269, 350, 530
SsiI CCGC 2 cut(s) 191, 357
SspMI CTAG 1 cut(s) 155
StyD4I CCNGG 2 cut(s) 524, 571
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 3 cut(s) 25, 97, 109
TaiI ACGT 1 cut(s) 653
TaqI TCGA 4 cut(s) 216, 228, 563, 621
TasI AATT 3 cut(s) 269, 350, 530
TatI WGTACW 1 cut(s) 5
TfiI GAWTC 2 cut(s) 458, 568
Tru1I TTAA 4 cut(s) 99, 336, 377, 609
Tru9I TTAA 4 cut(s) 99, 336, 377, 609
TscAI CASTG 3 cut(s) 112, 286, 318
TseFI GTSAC 2 cut(s) 31, 113
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 2 cut(s) 31, 113
TspDTI ATGAA 4 cut(s) 167, 239, 456, 581
TspRI CASTG 3 cut(s) 112, 286, 318
VpaK11BI GGWCC 2 cut(s) 405, 587
XapI RAATTY 2 cut(s) 350, 530
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 1 cut(s) 155
ZrmI AGTACT 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.