MD02G1305400.v1.1

reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000709
Physical Location & Seq
Forward (+)
35857053 .. 35861951
4899 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1305400.v1.1.491

Sequence Viewer

Length: 960 bp
ATGAGGAAGGTGGTGTGTGTAACAGGAGCCTCTGGTTACATAGCAGCATGGCTGGTGAAGCTCTTACTGGAACGAGGTTATATTGTCAAAGCCACTGTGCGTGAACCAAATGATTCAAAGAAAACAGAACACCTGCTCTCACTTGATGGAGCAAAAGAAAGGCTTCAATTGTTCAAAGCAAATTTATTAGAAGAAGGATCTTTCGACGCTGCAGTCGATGGATGTGAAGGTGTTTTTCATACAGCATCACCTGTACAATTTTCAGCCACTGATCCGCAGGCAGAACTAATTGACCCTGCAGTGAAGGGAACACTCAATGTTCTAAAATCGTGCGTAAAGTTTACTACTGTCAAGAGAGTGGTTTTAACATCTTCTATGGCTGCAGTCATAGTGAATGGAAGACCTTTGAACCCTGATGTGGTAGTTGACGAAACATGGTATTCGGATCAAGCTGTTTGTGCGCAGTTGAAGGAATGGTATTTTCTTTCAAAAACTATAGCTGAGGAGGCTGCTTGGAAATTTTCCAAAGAAAATGGGATTAACTTGGTTACAATAAACCCATCATATGTGATTGGTCCGCTCCTACAGCCAACTCTTAATCTCACTGTGAAGATGATTCTAAATCTCAAAAATGATATCCACGATGTAATCAGTTCAAATTATATATCTAGTGATGTTAGAGATGTTGCCTTTGCTCATGTTCAAGCATTTGAAGTCCCTTCGGCTAGTGGCAGATATTGTTTCGTTGGTCATGTTACACCCGTGTCCAAGGTTCTGAATATTTTACATGAACTCCATCCCACTTTTTTCCCAAAATGTGAGGATGACAAACCTTGTGAACCAGGCTTTCAAATATCAAAGCAAAAAGCAAAAAGTTTAGGAGTTGATTTTCTTCCATTGGAAGTAAGTCTTAGGGACACTATTGAAAGCCTCAAAGAGAAGGGCTTCCTCCAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

320

Amino Acids

35.21

Weight (kDa)

6.19

Isoelectric Point (pI)

24.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 2 - 101 1.7e-09 NmrA-like family
Epimerase PF01370 5 - 215 9.2e-20 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 7 - 238 5.7e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 7 - 192 1.3e-12 Male sterility protein
GDP_Man_Dehyd PF16363 7 - 126 4.8e-10 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 9 - 129 6.3e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 141
AasI GACNNNNNNGTC 1 cut(s) 212
Acc16I TGCGCA 1 cut(s) 462
Acc36I ACCTGC 1 cut(s) 141
AccBSI CCGCTC 1 cut(s) 580
AciI CCGC 2 cut(s) 275, 578
AclWI GGATC 3 cut(s) 205, 266, 453
AcsI RAATTY 2 cut(s) 181, 518
AfaI GTAC 1 cut(s) 255
AfiI CCNNNNNNNGG 1 cut(s) 418
AjnI CCWGG 1 cut(s) 841
AluBI AGCT 4 cut(s) 61, 452, 500, 955
AluI AGCT 4 cut(s) 61, 452, 500, 955
AlwI GGATC 3 cut(s) 205, 266, 453
AlwNI CAGNNNCTG 1 cut(s) 269
ApeKI GCWGC 4 cut(s) 44, 209, 380, 509
ApoI RAATTY 2 cut(s) 181, 518
Asp700I GAANNNNTTC 2 cut(s) 162, 944
AspLEI GCGC 1 cut(s) 463
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 2 cut(s) 67, 240
AvaII GGWCC 1 cut(s) 575
BbsI GAAGAC 1 cut(s) 406
BbvCI CCTCAGC 1 cut(s) 501
BbvI GCAGC 4 cut(s) 56, 196, 367, 496
BccI CCATC 4 cut(s) 140, 212, 568, 804
BciT130I CCWGG 1 cut(s) 843
BfaI CTAG 2 cut(s) 669, 726
BfmI CTRYAG 5 cut(s) 210, 297, 381, 495, 584
BfuAI ACCTGC 1 cut(s) 141
BisI GCNGC 4 cut(s) 45, 210, 381, 510
BlsI GCNGC 4 cut(s) 46, 211, 382, 511
Bme1390I CCNGG 1 cut(s) 843
Bme18I GGWCC 1 cut(s) 575
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 1 cut(s) 843
BmsI GCATC 1 cut(s) 254
BpiI GAAGAC 1 cut(s) 406
BpmI CTGGAG 1 cut(s) 935
Bpu10I CCTNAGC 1 cut(s) 501
BsaJI CCNNGG 1 cut(s) 768
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 1 cut(s) 418
Bse1I ACTGG 1 cut(s) 72
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 1 cut(s) 768
BseGI GGATG 3 cut(s) 227, 796, 829
BseLI CCNNNNNNNGG 1 cut(s) 418
BseMII CTCAG 1 cut(s) 492
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 1 cut(s) 518
BseXI GCAGC 4 cut(s) 56, 196, 367, 496
BslFI GGGAC 2 cut(s) 701, 929
BslI CCNNNNNNNGG 1 cut(s) 418
BsmFI GGGAC 2 cut(s) 701, 929
Bsp1407I TGTACA 1 cut(s) 253
Bsp143I GATC 3 cut(s) 197, 271, 445
BspACI CCGC 2 cut(s) 275, 578
BspCNI CTCAG 1 cut(s) 493
BspLI GGNNCC 1 cut(s) 28
BspMAI CTGCAG 3 cut(s) 214, 301, 385
BspMI ACCTGC 1 cut(s) 141
BspPI GGATC 3 cut(s) 205, 266, 453
BsrBI CCGCTC 1 cut(s) 580
BsrGI TGTACA 1 cut(s) 253
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 1 cut(s) 768
BssMI GATC 3 cut(s) 197, 271, 445
BssT1I CCWWGG 1 cut(s) 768
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 3 cut(s) 97, 349, 607
BstAUI TGTACA 1 cut(s) 253
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 2 cut(s) 501, 911
BstF5I GGATG 3 cut(s) 227, 796, 829
BstHHI GCGC 1 cut(s) 463
BstKTI GATC 3 cut(s) 200, 274, 448
BstMBI GATC 3 cut(s) 197, 271, 445
BstMWI GCNNNNNNNGC 4 cut(s) 58, 458, 506, 586
BstNI CCWGG 1 cut(s) 843
BstSCI CCNGG 1 cut(s) 841
BstSFI CTRYAG 5 cut(s) 210, 297, 381, 495, 584
BstV1I GCAGC 4 cut(s) 56, 196, 367, 496
BstV2I GAAGAC 1 cut(s) 406
BstX2I RGATCY 1 cut(s) 197
BstYI RGATCY 1 cut(s) 197
BtsCI GGATG 3 cut(s) 227, 796, 829
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 4 cut(s) 93, 267, 306, 603
BveI ACCTGC 1 cut(s) 141
Cac8I GCNNGC 1 cut(s) 279
CaiI CAGNNNCTG 1 cut(s) 269
CfoI GCGC 1 cut(s) 463
Cfr13I GGNCC 1 cut(s) 575
CseI GACGC 1 cut(s) 215
Csp6I GTAC 1 cut(s) 254
CviAII CATG 5 cut(s) 48, 435, 698, 752, 788
CviQI GTAC 1 cut(s) 254
DdeI CTNAG 2 cut(s) 501, 911
DpnI GATC 3 cut(s) 199, 273, 447
DpnII GATC 3 cut(s) 197, 271, 445
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
Eco130I CCWWGG 1 cut(s) 768
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 1 cut(s) 575
EcoRII CCWGG 1 cut(s) 841
EcoRV GATATC 1 cut(s) 637
EcoT14I CCWWGG 1 cut(s) 768
ErhI CCWWGG 1 cut(s) 768
FaeI CATG 5 cut(s) 51, 438, 701, 755, 791
FalI AAGNNNNNCTT 4 cut(s) 147, 179, 894, 926
FaqI GGGAC 2 cut(s) 701, 929
FatI CATG 5 cut(s) 47, 434, 697, 751, 787
FauNDI CATATG 1 cut(s) 565
Fnu4HI GCNGC 4 cut(s) 45, 210, 381, 510
FokI GGATG 3 cut(s) 234, 783, 836
Fsp4HI GCNGC 4 cut(s) 45, 210, 381, 510
FspBI CTAG 2 cut(s) 669, 726
FspI TGCGCA 1 cut(s) 462
GlaI GCGC 1 cut(s) 462
GluI GCNGC 4 cut(s) 45, 210, 381, 510
GsuI CTGGAG 1 cut(s) 935
HgaI GACGC 1 cut(s) 215
HhaI GCGC 1 cut(s) 463
Hin1II CATG 5 cut(s) 51, 438, 701, 755, 791
Hin6I GCGC 1 cut(s) 461
HinP1I GCGC 1 cut(s) 461
HincII GTYRAC 1 cut(s) 427
HindII GTYRAC 1 cut(s) 427
HinfI GANTC 2 cut(s) 113, 616
HphI GGTGA 2 cut(s) 67, 240
Hpy166II GTNNAC 4 cut(s) 104, 342, 427, 839
Hpy188I TCNGA 2 cut(s) 445, 777
Hpy188III TCNNGA 1 cut(s) 352
Hpy8I GTNNAC 4 cut(s) 104, 342, 427, 839
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 6 cut(s) 188, 221, 298, 463, 729, 934
HpyCH4III ACNGT 3 cut(s) 97, 349, 607
HpyCH4V TGCA 3 cut(s) 212, 299, 383
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 458, 506, 586
HpyF3I CTNAG 2 cut(s) 501, 911
Hsp92II CATG 5 cut(s) 51, 438, 701, 755, 791
HspAI GCGC 1 cut(s) 461
Kzo9I GATC 3 cut(s) 197, 271, 445
LmnI GCTCC 3 cut(s) 26, 149, 585
Lsp1109I GCAGC 4 cut(s) 56, 196, 367, 496
LweI GCATC 1 cut(s) 254
MaeI CTAG 2 cut(s) 669, 726
MaeIII GTNAC 4 cut(s) 19, 35, 547, 754
MalI GATC 3 cut(s) 199, 273, 447
MbiI CCGCTC 1 cut(s) 580
MboI GATC 3 cut(s) 197, 271, 445
MboII GAAGA 5 cut(s) 203, 363, 411, 622, 884
MfeI CAATTG 1 cut(s) 167
MflI RGATCY 1 cut(s) 197
MluCI AATT 6 cut(s) 167, 181, 257, 288, 518, 658
MnlI CCTC 7 cut(s) 40, 68, 496, 499, 814, 941, 959
MroXI GAANNNNTTC 2 cut(s) 162, 944
MseI TTAA 3 cut(s) 365, 540, 597
MspR9I CCNGG 1 cut(s) 843
MunI CAATTG 1 cut(s) 167
MvaI CCWGG 1 cut(s) 843
MwoI GCNNNNNNNGC 4 cut(s) 58, 458, 506, 586
NdeI CATATG 1 cut(s) 565
NdeII GATC 3 cut(s) 197, 271, 445
NlaIII CATG 5 cut(s) 51, 438, 701, 755, 791
NlaIV GGNNCC 1 cut(s) 28
NsbI TGCGCA 1 cut(s) 462
PaqCI CACCTGC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 1 cut(s) 213
PdmI GAANNNNTTC 2 cut(s) 162, 944
PfeI GAWTC 2 cut(s) 113, 616
PkrI GCNGC 4 cut(s) 46, 211, 382, 511
Psp6I CCWGG 1 cut(s) 841
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 1 cut(s) 28
PspPI GGNCC 1 cut(s) 575
PstI CTGCAG 3 cut(s) 214, 301, 385
PstNI CAGNNNCTG 1 cut(s) 269
PsuI RGATCY 1 cut(s) 197
RsaI GTAC 1 cut(s) 255
RsaNI GTAC 1 cut(s) 254
SaqAI TTAA 3 cut(s) 365, 540, 597
SatI GCNGC 4 cut(s) 45, 210, 381, 510
Sau3AI GATC 3 cut(s) 197, 271, 445
Sau96I GGNCC 1 cut(s) 575
ScrFI CCNGG 1 cut(s) 843
SfaNI GCATC 1 cut(s) 254
SfcI CTRYAG 5 cut(s) 210, 297, 381, 495, 584
SinI GGWCC 1 cut(s) 575
Sse9I AATT 6 cut(s) 167, 181, 257, 288, 518, 658
SsiI CCGC 2 cut(s) 275, 578
SspI AATATT 1 cut(s) 781
SspMI CTAG 2 cut(s) 669, 726
StyD4I CCNGG 1 cut(s) 841
StyI CCWWGG 1 cut(s) 768
TaaI ACNGT 3 cut(s) 97, 349, 607
TaqI TCGA 2 cut(s) 204, 216
TasI AATT 6 cut(s) 167, 181, 257, 288, 518, 658
TatI WGTACW 1 cut(s) 253
TfiI GAWTC 2 cut(s) 113, 616
Tru1I TTAA 3 cut(s) 365, 540, 597
Tru9I TTAA 3 cut(s) 365, 540, 597
TscAI CASTG 4 cut(s) 100, 274, 306, 610
TseI GCWGC 4 cut(s) 44, 209, 380, 509
TspDTI ATGAA 2 cut(s) 227, 804
TspRI CASTG 4 cut(s) 100, 274, 306, 610
VpaK11BI GGWCC 1 cut(s) 575
XapI RAATTY 2 cut(s) 181, 518
XmnI GAANNNNTTC 2 cut(s) 162, 944
XspI CTAG 2 cut(s) 669, 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.