Prupe.2G021300_v2.0.a1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000709
Physical Location & Seq
Reverse (-)
2085598 .. 2088295
2698 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G021300.2

Sequence Viewer

Length: 978 bp
ATGAGGGGAGGAAGGAAGGTGGTGTGTGTAACAGGAGCATCTGGTTTCATAGCATCGTGGTTGGTGAAGCTCTTATTACAAAGAGGTTATATTGTCAAAGCCACTGTTCGTGACCCAAATGATCCAAAGAAAACAGAACACTTACTCACACTTGATGGAGCAAAAGAAAGGCTACATTTGTTTCAAGCAGATTTGTTAGTTGAAGGATCTTTTGACCTTGCAGTTGATGGATGTGAAGGTGTTTTCCATACAGCATCCCCTGTAATATTTTCTTCCACTGACCCCCAGGAAGAATTACTATACCCTGCAGTGAAGGGCACACTAAATGTTCTGAAATCGTGCGTAAAATTCCCGACTGTCAAGAGGGTAGTTTTAACATCTTCAATGGCTTCAGTTCTGAACAATGGAAAACCTTTGACCCCTGATGTGGTAGTCGATGAAACATGGTTTTCAGATCCACTTGTTTGTGAGGATTTGGAGGCATGGTATTTTCTTTCAAAAACTTTAGCTGAGGAGGCTGCTTGGAAATTTGCTAAAGGAAATGGGATTGACTTGGTTACACTAAATCCGGCATATGTGATTGGTCCACTTTTGCAGCCAACTCTTAATGCCACTGTGGAAATGGTTCTGAATCTCAAAAAAGATATGCAAGAAGCAAACAAACCAAATTATATATCTACTGATGTTAGAGATGTTGCCTACACCCATATACAAGCATTTGAAGTTCCTTCAGCTAATGGAAGATACTGTTTAGTCGGACATGTTACACCCGTTTTCGAGGCTTTGAAGATTTTACAACAACTTCATCCCATTTTTTGCCCACCTGAAAACAGACTTGAGGATGGCAGTCCTGCTGAACCCACGTTTCAAGTATCGCAGGAAAAAGCAAAAAGCTTGGGAGTTAGTTTCCTTCCATTGGAAGTAAGTCTTAGGGACACTGTTGAAAGTCTCAAGGAGAAGGGCTTCCTCTCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

326

Amino Acids

35.8

Weight (kDa)

5.62

Isoelectric Point (pI)

28.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 3 cut(s) 116, 214, 449
AcsI RAATTY 2 cut(s) 347, 527
AcuI CTGAAG 2 cut(s) 375, 714
AfiI CCNNNNNNNGG 2 cut(s) 427, 916
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 8 cut(s) 185, 203, 384, 498, 722, 787, 869, 944
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 4 cut(s) 70, 509, 734, 894
AluI AGCT 4 cut(s) 70, 509, 734, 894
Alw26I GTCTC 1 cut(s) 953
AlwI GGATC 3 cut(s) 116, 214, 449
ApeKI GCWGC 2 cut(s) 518, 595
ApoI RAATTY 2 cut(s) 347, 527
Asp700I GAANNNNTTC 2 cut(s) 624, 962
AspS9I GGNCC 1 cut(s) 584
AsuHPI GGTGA 1 cut(s) 76
AvaII GGWCC 1 cut(s) 584
BaeGI GKGCMC 1 cut(s) 320
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 2 cut(s) 505, 607
BccI CCATC 3 cut(s) 149, 221, 836
BciT130I CCWGG 1 cut(s) 287
BcoDI GTCTC 1 cut(s) 953
BfmI CTRYAG 1 cut(s) 306
BisI GCNGC 2 cut(s) 519, 596
BlsI GCNGC 2 cut(s) 520, 597
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 1 cut(s) 584
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 3 cut(s) 47, 62, 263
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 3 cut(s) 857, 935, 955
BsaJI CCNNGG 1 cut(s) 285
BsaXI ACNNNNNCTCC 2 cut(s) 470, 500
Bsc4I CCNNNNNNNGG 2 cut(s) 427, 916
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 1 cut(s) 285
BseGI GGATG 4 cut(s) 236, 254, 805, 847
BseLI CCNNNNNNNGG 2 cut(s) 427, 916
BseMII CTCAG 1 cut(s) 501
BseRI GAGGAG 1 cut(s) 527
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 2 cut(s) 505, 607
BsiSI CCGG 1 cut(s) 569
BslFI GGGAC 1 cut(s) 947
BslI CCNNNNNNNGG 2 cut(s) 427, 916
BsmAI GTCTC 1 cut(s) 953
BsmFI GGGAC 1 cut(s) 947
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 3 cut(s) 121, 206, 454
BspCNI CTCAG 1 cut(s) 502
BspMAI CTGCAG 1 cut(s) 310
BspPI GGATC 3 cut(s) 116, 214, 449
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 3 cut(s) 121, 206, 454
Bst2UI CCWGG 1 cut(s) 287
Bst4CI ACNGT 5 cut(s) 106, 358, 616, 749, 940
BstDEI CTNAG 2 cut(s) 510, 929
BstF5I GGATG 4 cut(s) 236, 254, 805, 847
BstKTI GATC 3 cut(s) 124, 209, 457
BstMAI GTCTC 1 cut(s) 953
BstMBI GATC 3 cut(s) 121, 206, 454
BstMWI GCNNNNNNNGC 1 cut(s) 515
BstNI CCWGG 1 cut(s) 287
BstNSI RCATGY 1 cut(s) 764
BstSCI CCNGG 1 cut(s) 285
BstSFI CTRYAG 1 cut(s) 306
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 505, 607
BstX2I RGATCY 2 cut(s) 206, 454
BstYI RGATCY 2 cut(s) 206, 454
BtsCI GGATG 4 cut(s) 236, 254, 805, 847
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 5 cut(s) 102, 276, 315, 612, 936
Cfr13I GGNCC 1 cut(s) 584
CviAII CATG 3 cut(s) 444, 483, 761
DdeI CTNAG 2 cut(s) 510, 929
DpnI GATC 3 cut(s) 123, 208, 456
DpnII GATC 3 cut(s) 121, 206, 454
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 2 cut(s) 375, 714
EcoRII CCWGG 1 cut(s) 285
FaeI CATG 3 cut(s) 447, 486, 764
FalI AAGNNNNNCTT 2 cut(s) 912, 944
FaqI GGGAC 1 cut(s) 947
FatI CATG 3 cut(s) 443, 482, 760
FauNDI CATATG 1 cut(s) 574
Fnu4HI GCNGC 2 cut(s) 519, 596
FokI GGATG 4 cut(s) 241, 243, 792, 854
Fsp4HI GCNGC 2 cut(s) 519, 596
GluI GCNGC 2 cut(s) 519, 596
HapII CCGG 1 cut(s) 569
Hin1II CATG 3 cut(s) 447, 486, 764
HindIII AAGCTT 1 cut(s) 892
HinfI GANTC 1 cut(s) 631
HpaII CCGG 1 cut(s) 569
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 1 cut(s) 587
Hpy188I TCNGA 5 cut(s) 333, 399, 454, 630, 758
Hpy188III TCNNGA 4 cut(s) 110, 352, 361, 972
Hpy8I GTNNAC 1 cut(s) 587
HpyAV CCTTC 8 cut(s) 6, 10, 197, 230, 307, 738, 920, 952
HpyCH4III ACNGT 5 cut(s) 106, 358, 616, 749, 940
HpyCH4IV ACGT 1 cut(s) 863
HpyCH4V TGCA 4 cut(s) 221, 308, 595, 649
HpyF10VI GCNNNNNNNGC 1 cut(s) 515
HpyF3I CTNAG 2 cut(s) 510, 929
HpySE526I ACGT 1 cut(s) 863
Hsp92II CATG 3 cut(s) 447, 486, 764
Kzo9I GATC 3 cut(s) 121, 206, 454
LmnI GCTCC 2 cut(s) 35, 158
Lsp1109I GCAGC 2 cut(s) 505, 607
LweI GCATC 3 cut(s) 47, 62, 263
MaeII ACGT 1 cut(s) 863
MaeIII GTNAC 4 cut(s) 28, 110, 556, 763
MalI GATC 3 cut(s) 123, 208, 456
MboI GATC 3 cut(s) 121, 206, 454
MboII GAAGA 5 cut(s) 264, 302, 372, 753, 799
MflI RGATCY 2 cut(s) 206, 454
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 4 cut(s) 293, 347, 527, 667
MmeI TCCRAC 1 cut(s) 736
MnlI CCTC 9 cut(s) 77, 357, 463, 472, 505, 508, 772, 832, 977
MroXI GAANNNNTTC 2 cut(s) 624, 962
MseI TTAA 2 cut(s) 374, 606
MspI CCGG 1 cut(s) 569
MspR9I CCNGG 1 cut(s) 287
MvaI CCWGG 1 cut(s) 287
MwoI GCNNNNNNNGC 1 cut(s) 515
NdeI CATATG 1 cut(s) 574
NdeII GATC 3 cut(s) 121, 206, 454
NlaIII CATG 3 cut(s) 447, 486, 764
NmuCI GTSAC 1 cut(s) 110
NspI RCATGY 1 cut(s) 764
PciI ACATGT 1 cut(s) 760
PdmI GAANNNNTTC 2 cut(s) 624, 962
PfeI GAWTC 1 cut(s) 631
PkrI GCNGC 2 cut(s) 520, 597
PscI ACATGT 1 cut(s) 760
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspPI GGNCC 1 cut(s) 584
PstI CTGCAG 1 cut(s) 310
PsuI RGATCY 2 cut(s) 206, 454
SaqAI TTAA 2 cut(s) 374, 606
SatI GCNGC 2 cut(s) 519, 596
Sau3AI GATC 3 cut(s) 121, 206, 454
Sau96I GGNCC 1 cut(s) 584
ScrFI CCNGG 1 cut(s) 287
SduI GDGCHC 1 cut(s) 320
SfaNI GCATC 3 cut(s) 47, 62, 263
SfcI CTRYAG 1 cut(s) 306
SinI GGWCC 1 cut(s) 584
SmlI CTYRAG 3 cut(s) 836, 950, 970
SmoI CTYRAG 3 cut(s) 836, 950, 970
Sse9I AATT 4 cut(s) 293, 347, 527, 667
SspI AATATT 1 cut(s) 267
StyD4I CCNGG 1 cut(s) 285
TaaI ACNGT 5 cut(s) 106, 358, 616, 749, 940
TaiI ACGT 1 cut(s) 866
TaqI TCGA 2 cut(s) 435, 777
TasI AATT 4 cut(s) 293, 347, 527, 667
TfiI GAWTC 1 cut(s) 631
Tru1I TTAA 2 cut(s) 374, 606
Tru9I TTAA 2 cut(s) 374, 606
TscAI CASTG 5 cut(s) 109, 283, 315, 619, 943
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 2 cut(s) 518, 595
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 3 cut(s) 37, 453, 794
TspRI CASTG 5 cut(s) 109, 283, 315, 619, 943
VpaK11BI GGWCC 1 cut(s) 584
XapI RAATTY 2 cut(s) 347, 527
XceI RCATGY 1 cut(s) 764
XcmI CCANNNNNNNNNTGG 1 cut(s) 619
XmnI GAANNNNTTC 2 cut(s) 624, 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.