MD02G1304200.v1.1

reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000709
Physical Location & Seq
Forward (+)
35784209 .. 35787296
3088 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1304200.v1.1.491

Sequence Viewer

Length: 948 bp
ATGAGTGGAGAAACAAAGTTGGTGTGTGTTACAGGAGCCTCAGGTTACATAGCATCATGGTTGGTGAAGCTCTTATTGCGAAGGGGTTATACTGTCAAGGCCACTGTTCGTGACCCAAGTGATCCAAAGAAAACAGAACACTTGCTGTCACTAGATGGAGCAAAAGAAAGGCTTCATTTGTTCAAAGCAGATATATTAGAAGAAGGAGCTTTTGACACCGTAGTTGATGGATGTCAAGGCGTTTTTCATACAGCATCTCCTGTTCAAGTTTCAGCCACTGACCCACAGGGAACACTTAATGTTCTAAAATCATGCGGGAAATTTCCTACTGTCAAGAGAGTGGTTTTAACATCTTCTATGGCTTCAGTAATGATGAGTGGAAAACCTCTGACCTCTGATGTGGTTTTCAATGAAACATGGTATTCGGATCCACTTGTTTGTGAGAAGAACAAGCAATGGTATATGCTCTCAAAAACTTTAGCTGAGGAGGCTGCCTGGAAATACGCTAAAGCAAATGGAATTGACTTGGTTACAATGAATCCAGGGTTTGTGATAGGTCCACTCTTACAGCCAACTTTCAATCTCTCCATGGAGATAATTCAAACTCTCATAACCGGTACTGGAACACTACCCCTTTCAAGTTACCGGTTCGTTGATGTTAGGGATGTTGTCTCTGCTCATATTCTGGCATTTGAAGTTCCTTCAGCCAGTGGAAGATATTGTTTAGTTTCCAATGTTACACTAGCTTCCGAGGTTCTGAGGATTATACAAGAACTTTATCCCACTTTGCACCTTCCTGAAAAATGTGAGGTTGACAGTACTTCAAACCCAATATATCAAGTATCCACAGAGAAAGCAAAAGGTTTAGGAGTTAATTTCCTTCCTTTGGAAGTAAGTCTTAGGGACACAATTGAAAGCCTGAAGGAGAAGTGCTTCCTCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

316

Amino Acids

34.57

Weight (kDa)

6.54

Isoelectric Point (pI)

30.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 90 1.1e-08 NmrA-like family
Epimerase PF01370 8 - 236 1.1e-22 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 231 3.1e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 193 4.4e-11 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 190 7.1e-09 Male sterility protein
NAD_binding_10 PF13460 12 - 123 2.1e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 315
AclWI GGATC 3 cut(s) 116, 422, 435
AcsI RAATTY 1 cut(s) 320
AcuI CTGAAG 3 cut(s) 348, 687, 941
AfaI GTAC 2 cut(s) 619, 820
AfiI CCNNNNNNNGG 1 cut(s) 886
AgeI ACCGGT 2 cut(s) 614, 645
AgsI TTSAA 9 cut(s) 184, 266, 409, 580, 602, 639, 695, 825, 914
AjnI CCWGG 2 cut(s) 494, 541
AluBI AGCT 4 cut(s) 70, 209, 482, 746
AluI AGCT 4 cut(s) 70, 209, 482, 746
Alw26I GTCTC 1 cut(s) 676
AlwI GGATC 3 cut(s) 116, 422, 435
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 1 cut(s) 491
ApoI RAATTY 1 cut(s) 320
AsiGI ACCGGT 2 cut(s) 614, 645
Asp700I GAANNNNTTC 2 cut(s) 171, 932
AspS9I GGNCC 1 cut(s) 557
AsuHPI GGTGA 1 cut(s) 76
AvaII GGWCC 1 cut(s) 557
AxyI CCTNAGG 1 cut(s) 40
BamHI GGATCC 1 cut(s) 427
BarI GAAGNNNNNNTAC 2 cut(s) 73, 105
BbvCI CCTCAGC 1 cut(s) 483
BbvI GCAGC 1 cut(s) 478
BccI CCATC 2 cut(s) 149, 221
BciT130I CCWGG 2 cut(s) 496, 543
BciVI GTATCC 1 cut(s) 853
BcoDI GTCTC 1 cut(s) 676
BfaI CTAG 2 cut(s) 152, 743
BfuI GTATCC 1 cut(s) 853
BisI GCNGC 1 cut(s) 492
BlsI GCNGC 1 cut(s) 493
BmcAI AGTACT 1 cut(s) 820
Bme1390I CCNGG 2 cut(s) 496, 543
Bme18I GGWCC 1 cut(s) 557
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 2 cut(s) 37, 429
BmrFI CCNGG 2 cut(s) 496, 543
BmsI GCATC 2 cut(s) 62, 263
Bpu10I CCTNAGC 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 923
BsaJI CCNNGG 3 cut(s) 542, 588, 750
BsaWI WCCGGW 2 cut(s) 614, 645
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 1 cut(s) 886
Bse118I RCCGGY 2 cut(s) 614, 645
Bse1I ACTGG 2 cut(s) 625, 708
Bse21I CCTNAGG 1 cut(s) 40
Bse3DI GCAATG 1 cut(s) 461
BseBI CCWGG 2 cut(s) 496, 543
BseDI CCNNGG 3 cut(s) 542, 588, 750
BseGI GGATG 2 cut(s) 236, 670
BseLI CCNNNNNNNGG 1 cut(s) 886
BseMI GCAATG 1 cut(s) 461
BseMII CTCAG 3 cut(s) 54, 474, 749
BseNI ACTGG 2 cut(s) 625, 708
BseRI GAGGAG 1 cut(s) 500
BseXI GCAGC 1 cut(s) 478
BshFI GGCC 1 cut(s) 101
BshTI ACCGGT 2 cut(s) 614, 645
BsiSI CCGG 2 cut(s) 615, 646
BslFI GGGAC 1 cut(s) 917
BslI CCNNNNNNNGG 1 cut(s) 886
BsmAI GTCTC 1 cut(s) 676
BsmFI GGGAC 1 cut(s) 917
BsnI GGCC 1 cut(s) 101
Bsp143I GATC 2 cut(s) 121, 427
Bsp19I CCATGG 1 cut(s) 588
BspACI CCGC 1 cut(s) 315
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 3 cut(s) 53, 475, 750
BspLI GGNNCC 2 cut(s) 37, 429
BspPI GGATC 3 cut(s) 116, 422, 435
BsrDI GCAATG 1 cut(s) 461
BsrFI RCCGGY 2 cut(s) 614, 645
BsrI ACTGG 2 cut(s) 625, 708
BssAI RCCGGY 2 cut(s) 614, 645
BssECI CCNNGG 3 cut(s) 542, 588, 750
BssMI GATC 2 cut(s) 121, 427
BssT1I CCWWGG 1 cut(s) 588
Bst2UI CCWGG 2 cut(s) 496, 543
Bst4CI ACNGT 5 cut(s) 94, 106, 220, 331, 818
BstDEI CTNAG 4 cut(s) 40, 483, 758, 899
BstDSI CCRYGG 1 cut(s) 588
BstF5I GGATG 2 cut(s) 236, 670
BstKTI GATC 2 cut(s) 124, 430
BstMAI GTCTC 1 cut(s) 676
BstMBI GATC 2 cut(s) 121, 427
BstMWI GCNNNNNNNGC 2 cut(s) 76, 488
BstNI CCWGG 2 cut(s) 496, 543
BstSCI CCNGG 2 cut(s) 494, 541
BstV1I GCAGC 1 cut(s) 478
BstX2I RGATCY 1 cut(s) 427
BstYI RGATCY 1 cut(s) 427
Bsu36I CCTNAGG 1 cut(s) 40
BsuI GTATCC 1 cut(s) 853
BsuRI GGCC 1 cut(s) 101
BtgI CCRYGG 1 cut(s) 588
BtsCI GGATG 2 cut(s) 236, 670
BtsIMutI CAGTG 3 cut(s) 102, 276, 715
CaiI CAGNNNCTG 1 cut(s) 278
Cfr10I RCCGGY 2 cut(s) 614, 645
Cfr13I GGNCC 1 cut(s) 557
Csp6I GTAC 2 cut(s) 618, 819
CspAI ACCGGT 2 cut(s) 614, 645
CviAII CATG 4 cut(s) 57, 312, 417, 589
CviQI GTAC 2 cut(s) 618, 819
DdeI CTNAG 4 cut(s) 40, 483, 758, 899
DpnI GATC 2 cut(s) 123, 429
DpnII GATC 2 cut(s) 121, 427
Eco130I CCWWGG 1 cut(s) 588
Eco47I GGWCC 1 cut(s) 557
Eco57I CTGAAG 3 cut(s) 348, 687, 941
Eco81I CCTNAGG 1 cut(s) 40
EcoRII CCWGG 2 cut(s) 494, 541
EcoT14I CCWWGG 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 588
FaeI CATG 4 cut(s) 60, 315, 420, 592
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 882, 914
FaqI GGGAC 1 cut(s) 917
FatI CATG 4 cut(s) 56, 311, 416, 588
FauI CCCGC 1 cut(s) 308
Fnu4HI GCNGC 1 cut(s) 492
FokI GGATG 2 cut(s) 243, 677
Fsp4HI GCNGC 1 cut(s) 492
FspBI CTAG 2 cut(s) 152, 743
GluI GCNGC 1 cut(s) 492
HaeIII GGCC 1 cut(s) 101
HapII CCGG 2 cut(s) 615, 646
Hin1II CATG 4 cut(s) 60, 315, 420, 592
HincII GTYRAC 1 cut(s) 814
HindII GTYRAC 1 cut(s) 814
HinfI GANTC 1 cut(s) 538
HpaII CCGG 2 cut(s) 615, 646
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 2 cut(s) 560, 814
Hpy188I TCNGA 5 cut(s) 390, 397, 427, 751, 759
Hpy188III TCNNGA 4 cut(s) 110, 334, 797, 940
Hpy8I GTNNAC 2 cut(s) 560, 814
HpyAV CCTTC 6 cut(s) 75, 197, 711, 803, 890, 916
HpyCH4III ACNGT 5 cut(s) 94, 106, 220, 331, 818
HpyCH4V TGCA 1 cut(s) 790
HpyF10VI GCNNNNNNNGC 2 cut(s) 76, 488
HpyF3I CTNAG 4 cut(s) 40, 483, 758, 899
Hsp92II CATG 4 cut(s) 60, 315, 420, 592
Kzo9I GATC 2 cut(s) 121, 427
LmnI GCTCC 3 cut(s) 35, 158, 206
Lsp1109I GCAGC 1 cut(s) 478
LweI GCATC 2 cut(s) 62, 263
MaeI CTAG 2 cut(s) 152, 743
MaeIII GTNAC 7 cut(s) 28, 44, 110, 147, 529, 641, 736
MalI GATC 2 cut(s) 123, 429
MboI GATC 2 cut(s) 121, 427
MboII GAAGA 4 cut(s) 212, 345, 457, 726
MfeI CAATTG 1 cut(s) 909
MflI RGATCY 1 cut(s) 427
MluCI AATT 5 cut(s) 320, 519, 597, 874, 909
MnlI CCTC 9 cut(s) 49, 396, 403, 478, 481, 745, 753, 802, 947
MroXI GAANNNNTTC 2 cut(s) 171, 932
MseI TTAA 3 cut(s) 297, 347, 873
MspI CCGG 2 cut(s) 615, 646
MspR9I CCNGG 2 cut(s) 496, 543
MunI CAATTG 1 cut(s) 909
MvaI CCWGG 2 cut(s) 496, 543
MwoI GCNNNNNNNGC 2 cut(s) 76, 488
NcoI CCATGG 1 cut(s) 588
NdeII GATC 2 cut(s) 121, 427
NlaIII CATG 4 cut(s) 60, 315, 420, 592
NlaIV GGNNCC 2 cut(s) 37, 429
NmuCI GTSAC 2 cut(s) 110, 147
PdmI GAANNNNTTC 2 cut(s) 171, 932
PfeI GAWTC 1 cut(s) 538
PinAI ACCGGT 2 cut(s) 614, 645
PkrI GCNGC 1 cut(s) 493
Psp6I CCWGG 2 cut(s) 494, 541
PspGI CCWGG 2 cut(s) 494, 541
PspN4I GGNNCC 2 cut(s) 37, 429
PspPI GGNCC 1 cut(s) 557
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 427
RsaI GTAC 2 cut(s) 619, 820
RsaNI GTAC 2 cut(s) 618, 819
SaqAI TTAA 3 cut(s) 297, 347, 873
SatI GCNGC 1 cut(s) 492
Sau3AI GATC 2 cut(s) 121, 427
Sau96I GGNCC 1 cut(s) 557
ScaI AGTACT 1 cut(s) 820
ScrFI CCNGG 2 cut(s) 496, 543
SfaNI GCATC 2 cut(s) 62, 263
SinI GGWCC 1 cut(s) 557
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
Sse9I AATT 5 cut(s) 320, 519, 597, 874, 909
SsiI CCGC 1 cut(s) 315
SspMI CTAG 2 cut(s) 152, 743
StyD4I CCNGG 2 cut(s) 494, 541
StyI CCWWGG 1 cut(s) 588
TaaI ACNGT 5 cut(s) 94, 106, 220, 331, 818
TasI AATT 5 cut(s) 320, 519, 597, 874, 909
TatI WGTACW 1 cut(s) 818
TfiI GAWTC 1 cut(s) 538
Tru1I TTAA 3 cut(s) 297, 347, 873
Tru9I TTAA 3 cut(s) 297, 347, 873
TscAI CASTG 3 cut(s) 109, 283, 715
TseFI GTSAC 2 cut(s) 110, 147
TseI GCWGC 1 cut(s) 491
Tsp45I GTSAC 2 cut(s) 110, 147
TspDTI ATGAA 4 cut(s) 164, 236, 426, 551
TspRI CASTG 3 cut(s) 109, 283, 715
VpaK11BI GGWCC 1 cut(s) 557
XapI RAATTY 1 cut(s) 320
XmnI GAANNNNTTC 2 cut(s) 171, 932
XspI CTAG 2 cut(s) 152, 743
ZrmI AGTACT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.