MD07G1021200.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000709
Physical Location & Seq
Reverse (-)
1795801 .. 1798261
2461 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1021200.v1.1.491

Sequence Viewer

Length: 966 bp
ATGAGTACTGGACAAGGGAAGACTGTATGTGTAACAGGAGCGTCTGGTTACATAGCATCATGGCTGGTGAAGCTCTTACTGCAGAAGGGTTATACTGTCAAAGCCACTGTTCGTGATCCAAAGGATCCAAAGAAAACAGAACACTTGCGCTCACTAGACGGGGCAAAAGAAAGACTTCATTTGTTCAAAGCAGACTTATTAGAAGAAGGAGCTTTCGATGCTGCCGTCGATGGATGCCAAGGTGTTTTTCATACAGCATCCCCTGTACTGATTTCAGTTACTGACCCTCAGGCCGAATTAATTGACCCTGCAGTGAAGGGAACACTTAATGTTCTACAATCCTGCGCAAAATTTCGCAGTGTCATGAGAGTGGTTTTAACATCTTCAATTGCTACAGTCATGATGAGTGGAACACCTCTGACTTCTGATGTGGTTTTGGATGAAACATGGTATTCGGATAAACTTTTTTGTGAGGAGCACAAGATATGGTATGTGCTCTCAAAAATATTAGCCGAGGAGGCTGGCTGGAAATTTGCTGAAGGAAATGGAATCGATATGGTGTCAATGAATCCTCGGTTGTGTATTGGTCCACTCTTAAATCCAACTCTTATGACCTCCGTTGAGCAGCTTCTGAATGTCATGAGTGGTACCCCAACACCGTTTCTAAATTACCCATTTGTAGACGTTAGAGATGTTGCCTCTGCTCATATTCAAGAATTTGAAGTTCCTTCAGCTAGTGGCAGATATTGTTTAGTTGGCCACGTTGTTGACGGATTCGATACCCTAAAGATTTTACGACAACTCTACCCTACTTTGACCCTTGCTGAGGCTGTCAATCCGTCAGACTCAAAGTATCAAGTGTCCCAGGAGAAAGCAAAAGGTTTGGGAATCACTTTCCTTCCTCTGGAAACAAGTCTTAGGGACACTATTGAAAGCCTCAAGGAGAAGGGCTTCATCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

322

Amino Acids

35.15

Weight (kDa)

5.75

Isoelectric Point (pI)

30.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 105 2.9e-10 NmrA-like family
KR PF08659 8 - 135 5.3e-06 KR domain
Epimerase PF01370 9 - 239 1.4e-20 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 197 2.7e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 129 4.3e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 185 1.9e-08 Male sterility protein
NAD_binding_10 PF13460 13 - 133 1.8e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 346
Acc65I GGTACC 1 cut(s) 647
AccB1I GGYRCC 1 cut(s) 647
AccI GTMKAC 1 cut(s) 681
AclWI GGATC 3 cut(s) 110, 119, 132
AcoI YGGCCR 1 cut(s) 757
AcsI RAATTY 3 cut(s) 350, 530, 716
AcuI CTGAAG 2 cut(s) 558, 714
AfaI GTAC 3 cut(s) 7, 267, 649
AfiI CCNNNNNNNGG 2 cut(s) 826, 904
AgsI TTSAA 5 cut(s) 187, 387, 713, 722, 932
AjnI CCWGG 1 cut(s) 864
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AluBI AGCT 4 cut(s) 73, 212, 628, 734
AluI AGCT 4 cut(s) 73, 212, 628, 734
Alw21I GWGCWC 2 cut(s) 480, 498
AlwI GGATC 3 cut(s) 110, 119, 132
AlwNI CAGNNNCTG 2 cut(s) 281, 631
AoxI GGCC 2 cut(s) 291, 757
ApeKI GCWGC 2 cut(s) 221, 625
ApoI RAATTY 3 cut(s) 350, 530, 716
AseI ATTAAT 1 cut(s) 299
Asp700I GAANNNNTTC 2 cut(s) 174, 950
Asp718I GGTACC 1 cut(s) 647
AspLEI GCGC 2 cut(s) 150, 347
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 1 cut(s) 79
AvaII GGWCC 1 cut(s) 587
AxyI CCTNAGG 1 cut(s) 288
BalI TGGCCA 1 cut(s) 759
BamHI GGATCC 1 cut(s) 124
BanI GGYRCC 1 cut(s) 647
BbsI GAAGAC 1 cut(s) 26
Bbv12I GWGCWC 2 cut(s) 480, 498
BbvCI CCTCAGC 1 cut(s) 825
BbvI GCAGC 2 cut(s) 208, 637
BccI CCATC 1 cut(s) 224
BceAI ACGGC 1 cut(s) 209
BciT130I CCWGG 1 cut(s) 866
BfaI CTAG 2 cut(s) 155, 735
BfmI CTRYAG 3 cut(s) 80, 309, 393
BglI GCCNNNNNGGC 1 cut(s) 518
BisI GCNGC 2 cut(s) 222, 626
BlsI GCNGC 2 cut(s) 223, 627
BmcAI AGTACT 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 866
Bme18I GGWCC 1 cut(s) 587
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 2 cut(s) 126, 649
BmrFI CCNGG 1 cut(s) 866
BmsI GCATC 4 cut(s) 65, 208, 224, 266
BpiI GAAGAC 1 cut(s) 26
Bpu10I CCTNAGC 1 cut(s) 825
BpuEI CTTGAG 1 cut(s) 923
Bsa29I ATCGAT 1 cut(s) 552
BsaJI CCNNGG 4 cut(s) 238, 513, 572, 864
Bsc4I CCNNNNNNNGG 2 cut(s) 826, 904
Bse1I ACTGG 1 cut(s) 13
Bse21I CCTNAGG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 866
BseCI ATCGAT 1 cut(s) 552
BseDI CCNNGG 4 cut(s) 238, 513, 572, 864
BseGI GGATG 3 cut(s) 239, 257, 445
BseLI CCNNNNNNNGG 2 cut(s) 826, 904
BseMII CTCAG 2 cut(s) 302, 816
BseNI ACTGG 1 cut(s) 13
BseRI GAGGAG 2 cut(s) 488, 530
BseXI GCAGC 2 cut(s) 208, 637
BshFI GGCC 2 cut(s) 293, 759
BshNI GGYRCC 1 cut(s) 647
BshVI ATCGAT 1 cut(s) 552
BsiHKAI GWGCWC 2 cut(s) 480, 498
BslFI GGGAC 2 cut(s) 847, 935
BslI CCNNNNNNNGG 2 cut(s) 826, 904
BsmFI GGGAC 2 cut(s) 847, 935
BsnI GGCC 2 cut(s) 293, 759
Bsp1286I GDGCHC 2 cut(s) 480, 498
Bsp143I GATC 2 cut(s) 115, 124
BspANI GGCC 2 cut(s) 293, 759
BspCNI CTCAG 2 cut(s) 301, 817
BspDI ATCGAT 1 cut(s) 552
BspHI TCATGA 3 cut(s) 363, 399, 639
BspLI GGNNCC 2 cut(s) 126, 649
BspMAI CTGCAG 2 cut(s) 84, 313
BspPI GGATC 3 cut(s) 110, 119, 132
BspT107I GGYRCC 1 cut(s) 647
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 4 cut(s) 238, 513, 572, 864
BssMI GATC 2 cut(s) 115, 124
BssT1I CCWWGG 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 866
Bst4CI ACNGT 5 cut(s) 25, 97, 109, 397, 660
BstC8I GCNNGC 1 cut(s) 523
BstDEI CTNAG 3 cut(s) 288, 825, 917
BstENI CCTNNNNNAGG 1 cut(s) 824
BstF5I GGATG 3 cut(s) 239, 257, 445
BstHHI GCGC 2 cut(s) 150, 347
BstKTI GATC 2 cut(s) 118, 127
BstMBI GATC 2 cut(s) 115, 124
BstMWI GCNNNNNNNGC 4 cut(s) 70, 79, 218, 518
BstNI CCWGG 1 cut(s) 866
BstSCI CCNGG 1 cut(s) 864
BstSFI CTRYAG 3 cut(s) 80, 309, 393
BstV1I GCAGC 2 cut(s) 208, 637
BstV2I GAAGAC 1 cut(s) 26
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
Bsu15I ATCGAT 1 cut(s) 552
Bsu36I CCTNAGG 1 cut(s) 288
BsuRI GGCC 2 cut(s) 293, 759
BsuTUI ATCGAT 1 cut(s) 552
BtsCI GGATG 3 cut(s) 239, 257, 445
BtsI GCAGTG 2 cut(s) 318, 364
BtsIMutI CAGTG 3 cut(s) 105, 318, 364
Cac8I GCNNGC 1 cut(s) 523
CaiI CAGNNNCTG 2 cut(s) 281, 631
CciI TCATGA 3 cut(s) 363, 399, 639
CfoI GCGC 2 cut(s) 150, 347
Cfr13I GGNCC 1 cut(s) 587
ClaI ATCGAT 1 cut(s) 552
CseI GACGC 1 cut(s) 30
Csp6I GTAC 3 cut(s) 6, 266, 648
CviAII CATG 5 cut(s) 60, 364, 400, 447, 640
CviQI GTAC 3 cut(s) 6, 266, 648
DdeI CTNAG 3 cut(s) 288, 825, 917
DpnI GATC 2 cut(s) 117, 126
DpnII GATC 2 cut(s) 115, 124
EaeI YGGCCR 1 cut(s) 757
Eco130I CCWWGG 1 cut(s) 238
Eco47I GGWCC 1 cut(s) 587
Eco57I CTGAAG 2 cut(s) 558, 714
Eco81I CCTNAGG 1 cut(s) 288
EcoNI CCTNNNNNAGG 1 cut(s) 824
EcoRII CCWGG 1 cut(s) 864
EcoT14I CCWWGG 1 cut(s) 238
ErhI CCWWGG 1 cut(s) 238
FaeI CATG 5 cut(s) 63, 367, 403, 450, 643
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 2 cut(s) 847, 935
FatI CATG 5 cut(s) 59, 363, 399, 446, 639
FblI GTMKAC 1 cut(s) 681
Fnu4HI GCNGC 2 cut(s) 222, 626
FokI GGATG 3 cut(s) 244, 246, 452
Fsp4HI GCNGC 2 cut(s) 222, 626
FspBI CTAG 2 cut(s) 155, 735
FspI TGCGCA 1 cut(s) 346
GlaI GCGC 2 cut(s) 149, 346
GluI GCNGC 2 cut(s) 222, 626
HaeIII GGCC 2 cut(s) 293, 759
HgaI GACGC 1 cut(s) 30
HhaI GCGC 2 cut(s) 150, 347
Hin1II CATG 5 cut(s) 63, 367, 403, 450, 643
Hin6I GCGC 2 cut(s) 148, 345
HinP1I GCGC 2 cut(s) 148, 345
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 5 cut(s) 549, 568, 774, 845, 888
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 3 cut(s) 590, 682, 769
Hpy188I TCNGA 5 cut(s) 420, 427, 457, 633, 844
Hpy188III TCNNGA 7 cut(s) 113, 364, 400, 640, 713, 905, 958
Hpy8I GTNNAC 3 cut(s) 590, 682, 769
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 7 cut(s) 79, 200, 310, 533, 738, 908, 940
HpyCH4III ACNGT 5 cut(s) 25, 97, 109, 397, 660
HpyCH4IV ACGT 2 cut(s) 684, 762
HpyCH4V TGCA 2 cut(s) 82, 311
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 79, 218, 518
HpyF3I CTNAG 3 cut(s) 288, 825, 917
HpySE526I ACGT 2 cut(s) 684, 762
Hsp92II CATG 5 cut(s) 63, 367, 403, 450, 643
HspAI GCGC 2 cut(s) 148, 345
KpnI GGTACC 1 cut(s) 651
Kzo9I GATC 2 cut(s) 115, 124
LmnI GCTCC 3 cut(s) 38, 209, 475
Lsp1109I GCAGC 2 cut(s) 208, 637
LweI GCATC 4 cut(s) 65, 208, 224, 266
MaeI CTAG 2 cut(s) 155, 735
MaeII ACGT 2 cut(s) 684, 762
MaeIII GTNAC 3 cut(s) 31, 47, 277
MalI GATC 2 cut(s) 117, 126
MboI GATC 2 cut(s) 115, 124
MboII GAAGA 3 cut(s) 31, 215, 375
MfeI CAATTG 1 cut(s) 387
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 2 cut(s) 480, 498
MlsI TGGCCA 1 cut(s) 759
MluCI AATT 7 cut(s) 296, 300, 350, 387, 530, 667, 716
MluNI TGGCCA 1 cut(s) 759
MlyI GAGTC 1 cut(s) 839
MmeI TCCRAC 1 cut(s) 626
Mox20I TGGCCA 1 cut(s) 759
MroXI GAANNNNTTC 2 cut(s) 174, 950
MscI TGGCCA 1 cut(s) 759
MseI TTAA 4 cut(s) 299, 327, 377, 596
MslI CAYNNNNRTG 1 cut(s) 368
Msp20I TGGCCA 1 cut(s) 759
MspR9I CCNGG 1 cut(s) 866
MunI CAATTG 1 cut(s) 387
MvaI CCWGG 1 cut(s) 866
MwoI GCNNNNNNNGC 4 cut(s) 70, 79, 218, 518
NdeII GATC 2 cut(s) 115, 124
NlaIII CATG 5 cut(s) 63, 367, 403, 450, 643
NlaIV GGNNCC 2 cut(s) 126, 649
NmeAIII GCCGAG 1 cut(s) 538
NsbI TGCGCA 1 cut(s) 346
PagI TCATGA 3 cut(s) 363, 399, 639
PcsI WCGNNNNNNNCGW 1 cut(s) 222
PdmI GAANNNNTTC 2 cut(s) 174, 950
PfeI GAWTC 4 cut(s) 549, 568, 774, 888
PkrI GCNGC 2 cut(s) 223, 627
PleI GAGTC 1 cut(s) 839
PpsI GAGTC 1 cut(s) 839
PshBI ATTAAT 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 864
PspGI CCWGG 1 cut(s) 864
PspN4I GGNNCC 2 cut(s) 126, 649
PspPI GGNCC 1 cut(s) 587
PstI CTGCAG 2 cut(s) 84, 313
PstNI CAGNNNCTG 2 cut(s) 281, 631
PsuI RGATCY 1 cut(s) 124
RsaI GTAC 3 cut(s) 7, 267, 649
RsaNI GTAC 3 cut(s) 6, 266, 648
RseI CAYNNNNRTG 1 cut(s) 368
SaqAI TTAA 4 cut(s) 299, 327, 377, 596
SatI GCNGC 2 cut(s) 222, 626
Sau3AI GATC 2 cut(s) 115, 124
Sau96I GGNCC 1 cut(s) 587
ScaI AGTACT 1 cut(s) 7
SchI GAGTC 1 cut(s) 839
ScrFI CCNGG 1 cut(s) 866
SduI GDGCHC 2 cut(s) 480, 498
SfaNI GCATC 4 cut(s) 65, 208, 224, 266
SfcI CTRYAG 3 cut(s) 80, 309, 393
SinI GGWCC 1 cut(s) 587
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 938
SmoI CTYRAG 1 cut(s) 938
Sse9I AATT 7 cut(s) 296, 300, 350, 387, 530, 667, 716
SspI AATATT 1 cut(s) 507
SspMI CTAG 2 cut(s) 155, 735
StyD4I CCNGG 1 cut(s) 864
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 5 cut(s) 25, 97, 109, 397, 660
TaiI ACGT 2 cut(s) 687, 765
TaqI TCGA 4 cut(s) 216, 228, 552, 777
TasI AATT 7 cut(s) 296, 300, 350, 387, 530, 667, 716
TatI WGTACW 2 cut(s) 5, 265
TfiI GAWTC 4 cut(s) 549, 568, 774, 888
Tru1I TTAA 4 cut(s) 299, 327, 377, 596
Tru9I TTAA 4 cut(s) 299, 327, 377, 596
TscAI CASTG 3 cut(s) 112, 318, 364
TseI GCWGC 2 cut(s) 221, 625
TspDTI ATGAA 5 cut(s) 167, 239, 456, 581, 943
TspGWI ACGGA 3 cut(s) 607, 786, 828
TspRI CASTG 3 cut(s) 112, 318, 364
VpaK11BI GGWCC 1 cut(s) 587
VspI ATTAAT 1 cut(s) 299
XagI CCTNNNNNAGG 1 cut(s) 824
XapI RAATTY 3 cut(s) 350, 530, 716
XmiI GTMKAC 1 cut(s) 681
XmnI GAANNNNTTC 2 cut(s) 174, 950
XspI CTAG 2 cut(s) 155, 735
ZrmI AGTACT 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.