pycom07g01570

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000709
Physical Location & Seq
Reverse (-)
1243720 .. 1245334
1615 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g01570.4

Sequence Viewer

Length: 966 bp
ATGAGTACCGGAGAATCAAAGACTGTATGTGTAACAGGAGCTTCTGGTTACATAGCATCATCGCTGGTGAAGCTCTTACTGCAAAAGGGTTATACCGTCAAAGCCACTGTTCGCGATCCAAATGATGCAAAGAAAACAGAACACTTGCTCTCATTAGATGGAGCCAAGGAGAGGCTTCATTTGTTCAAAGCGGATTTATTTGAAGATCGATCATTTGACGCCGTAATTGATGGATGTGTAGGTGTTTTTCATACAGCAACGCCTGTACAATTTTCAGCCACTGACCCACAGGTAGAAATAATTGAGCCTGCAGTGAAAGGAACACTTAATGTTCTAAAGTCATGCGCAAAATTCCCCGCTGTCAAGAGAGTGGTTTTAACATCTTCTCTGGCTGCAGTAAAGGTGAATGGAAAACCTCTGACCTCTGATGTGGTTATTGATGAAACATGGTACTCGGATCCACTGTTTTGCGAAGAGATCAAGCAATGGTATCCTCTCTCAAAAACTTTAGCTGAGGAGGCAGCCTGGAAATTTGCCAAAGGAAATGGGATTGACTTGGTAGCAATACATCCGGGGTTTGCGATTGGTCCACTCTTACGGCCAACTCTTAATCTCTCCGTTGGGTTTCTTCTGAATTTAATGAGTGGTATCAAAACACCATTTATAGACCACGCATTCGTGGACGTTAGAGATATTGCCTTTGCTCATATTCAAGCATTTGAAGTTCCTTCAGCTAGTGGTAGATATTGTTTAGTAGCACAAGTTGCTGATGGTCCCGATACCCTAAAGATTTTACAAGAGCTTTACCCCACATTGAGCCTTCCTGAGCCTGGCAATCCTTCTGGCTCCAAGTTTCAAGTGTCCAAGGAGAAAGCAAAATGTTTGGGAATCACTTTCCGTCCTCTGGAAACGAGTCTCAAGGACACTGTTGAAAGCCTCAAGGAGAAGGGCTTCCTCAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

322

Amino Acids

34.87

Weight (kDa)

7.0

Isoelectric Point (pI)

26.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 346
AccII CGCG 1 cut(s) 114
AciI CCGC 2 cut(s) 191, 357
AclWI GGATC 3 cut(s) 110, 452, 465
AcoI YGGCCR 1 cut(s) 599
AcsI RAATTY 3 cut(s) 350, 530, 634
AcuI CTGAAG 1 cut(s) 714
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 3 cut(s) 7, 267, 452
AfiI CCNNNNNNNGG 3 cut(s) 171, 830, 904
AgsI TTSAA 6 cut(s) 187, 203, 713, 722, 857, 932
AjnI CCWGG 2 cut(s) 524, 829
AluBI AGCT 5 cut(s) 41, 73, 512, 734, 802
AluI AGCT 5 cut(s) 41, 73, 512, 734, 802
Alw26I GTCTC 1 cut(s) 920
AlwI GGATC 3 cut(s) 110, 452, 465
AlwNI CAGNNNCTG 1 cut(s) 281
AoxI GGCC 1 cut(s) 599
ApeKI GCWGC 2 cut(s) 392, 521
ApoI RAATTY 3 cut(s) 350, 530, 634
ArsI GACNNNNNNTTYG 2 cut(s) 659, 691
Asp700I GAANNNNTTC 1 cut(s) 950
AspLEI GCGC 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 587, 773
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 2 cut(s) 79, 415
AvaII GGWCC 2 cut(s) 587, 773
BamHI GGATCC 1 cut(s) 457
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 2 cut(s) 379, 533
BccI CCATC 3 cut(s) 152, 224, 764
BceAI ACGGC 2 cut(s) 206, 614
BciT130I CCWGG 2 cut(s) 526, 831
BciVI GTATCC 1 cut(s) 501
BcnI CCSGG 1 cut(s) 573
BcoDI GTCTC 1 cut(s) 920
BfaI CTAG 1 cut(s) 735
BfmI CTRYAG 2 cut(s) 309, 393
BfuI GTATCC 1 cut(s) 501
BisI GCNGC 2 cut(s) 393, 522
BlsI GCNGC 2 cut(s) 394, 523
Bme1390I CCNGG 3 cut(s) 526, 573, 831
Bme18I GGWCC 2 cut(s) 587, 773
BmgT120I GGNCC 2 cut(s) 587, 773
BmiI GGNNCC 4 cut(s) 163, 459, 775, 847
BmrFI CCNGG 3 cut(s) 526, 573, 831
BmsI GCATC 2 cut(s) 65, 115
Bpu10I CCTNAGC 2 cut(s) 513, 825
BpuEI CTTGAG 3 cut(s) 902, 923, 941
BpuMI CCSGG 1 cut(s) 573
Bsa29I ATCGAT 1 cut(s) 208
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 165, 572, 864
BsaWI WCCGGW 1 cut(s) 8
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 830, 904
Bse3DI GCAATG 1 cut(s) 491
BseBI CCWGG 2 cut(s) 526, 831
BseCI ATCGAT 1 cut(s) 208
BseDI CCNNGG 3 cut(s) 165, 572, 864
BseGI GGATG 2 cut(s) 239, 568
BseLI CCNNNNNNNGG 3 cut(s) 171, 830, 904
BseMI GCAATG 1 cut(s) 491
BseMII CTCAG 2 cut(s) 504, 816
BseRI GAGGAG 1 cut(s) 530
BseXI GCAGC 2 cut(s) 379, 533
Bsh1236I CGCG 1 cut(s) 114
BshFI GGCC 1 cut(s) 601
BshVI ATCGAT 1 cut(s) 208
BsiSI CCGG 2 cut(s) 9, 572
BslFI GGGAC 1 cut(s) 759
BslI CCNNNNNNNGG 3 cut(s) 171, 830, 904
BsmAI GTCTC 1 cut(s) 920
BsmFI GGGAC 1 cut(s) 759
BsmI GAATGC 1 cut(s) 674
BsnI GGCC 1 cut(s) 601
Bsp1407I TGTACA 1 cut(s) 265
Bsp143I GATC 5 cut(s) 115, 205, 209, 457, 477
Bsp68I TCGCGA 1 cut(s) 114
BspACI CCGC 2 cut(s) 191, 357
BspANI GGCC 1 cut(s) 601
BspCNI CTCAG 2 cut(s) 505, 817
BspDI ATCGAT 1 cut(s) 208
BspFNI CGCG 1 cut(s) 114
BspLI GGNNCC 4 cut(s) 163, 459, 775, 847
BspMAI CTGCAG 2 cut(s) 313, 397
BspPI GGATC 3 cut(s) 110, 452, 465
BsrDI GCAATG 1 cut(s) 491
BsrGI TGTACA 1 cut(s) 265
BssECI CCNNGG 3 cut(s) 165, 572, 864
BssMI GATC 5 cut(s) 115, 205, 209, 457, 477
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 2 cut(s) 165, 864
Bst2UI CCWGG 2 cut(s) 526, 831
Bst4CI ACNGT 5 cut(s) 25, 97, 109, 465, 928
Bst6I CTCTTC 1 cut(s) 468
BstACI GRCGYC 1 cut(s) 219
BstAPI GCANNNNNTGC 1 cut(s) 764
BstAUI TGTACA 1 cut(s) 265
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 2 cut(s) 513, 825
BstF5I GGATG 2 cut(s) 239, 568
BstFNI CGCG 1 cut(s) 114
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 5 cut(s) 118, 208, 212, 460, 480
BstMAI GTCTC 1 cut(s) 920
BstMBI GATC 5 cut(s) 115, 205, 209, 457, 477
BstMWI GCNNNNNNNGC 4 cut(s) 70, 79, 518, 764
BstNI CCWGG 2 cut(s) 526, 831
BstSCI CCNGG 3 cut(s) 524, 571, 829
BstSFI CTRYAG 2 cut(s) 309, 393
BstUI CGCG 1 cut(s) 114
BstV1I GCAGC 2 cut(s) 379, 533
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
Bsu15I ATCGAT 1 cut(s) 208
BsuI GTATCC 1 cut(s) 501
BsuRI GGCC 1 cut(s) 601
BsuTUI ATCGAT 1 cut(s) 208
BtgZI GCGATG 1 cut(s) 45
BtsCI GGATG 2 cut(s) 239, 568
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 5 cut(s) 105, 279, 318, 461, 924
BtuMI TCGCGA 1 cut(s) 114
Cac8I GCNNGC 1 cut(s) 309
CaiI CAGNNNCTG 1 cut(s) 281
CfoI GCGC 1 cut(s) 347
Cfr13I GGNCC 2 cut(s) 587, 773
ClaI ATCGAT 1 cut(s) 208
CseI GACGC 1 cut(s) 227
Csp6I GTAC 3 cut(s) 6, 266, 451
CviAII CATG 2 cut(s) 342, 447
CviQI GTAC 3 cut(s) 6, 266, 451
DdeI CTNAG 2 cut(s) 513, 825
DpnI GATC 5 cut(s) 117, 207, 211, 459, 479
DpnII GATC 5 cut(s) 115, 205, 209, 457, 477
EaeI YGGCCR 1 cut(s) 599
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
Eco130I CCWWGG 2 cut(s) 165, 864
Eco47I GGWCC 2 cut(s) 587, 773
Eco57I CTGAAG 1 cut(s) 714
EcoRII CCWGG 2 cut(s) 524, 829
EcoT14I CCWWGG 2 cut(s) 165, 864
ErhI CCWWGG 2 cut(s) 165, 864
FaeI CATG 2 cut(s) 345, 450
FaiI YATR 8 cut(s) 28, 53, 93, 252, 343, 448, 665, 708
FalI AAGNNNNNCTT 2 cut(s) 309, 341
FaqI GGGAC 1 cut(s) 759
FatI CATG 2 cut(s) 341, 446
FauI CCCGC 1 cut(s) 364
Fnu4HI GCNGC 2 cut(s) 393, 522
FokI GGATG 2 cut(s) 246, 555
Fsp4HI GCNGC 2 cut(s) 393, 522
FspBI CTAG 1 cut(s) 735
FspI TGCGCA 1 cut(s) 346
GlaI GCGC 1 cut(s) 346
GluI GCNGC 2 cut(s) 393, 522
HaeIII GGCC 1 cut(s) 601
HapII CCGG 2 cut(s) 9, 572
HgaI GACGC 1 cut(s) 227
HhaI GCGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 2 cut(s) 345, 450
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HinfI GANTC 3 cut(s) 14, 888, 913
HpaII CCGG 2 cut(s) 9, 572
HphI GGTGA 2 cut(s) 79, 415
Hpy166II GTNNAC 2 cut(s) 590, 682
Hpy188I TCNGA 4 cut(s) 420, 427, 457, 633
Hpy188III TCNNGA 5 cut(s) 113, 364, 776, 824, 905
Hpy8I GTNNAC 2 cut(s) 590, 682
HpyAV CCTTC 4 cut(s) 738, 830, 849, 940
HpyCH4III ACNGT 5 cut(s) 25, 97, 109, 465, 928
HpyCH4IV ACGT 1 cut(s) 684
HpyCH4V TGCA 4 cut(s) 82, 128, 311, 395
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 79, 518, 764
HpyF3I CTNAG 2 cut(s) 513, 825
HpySE526I ACGT 1 cut(s) 684
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 2 cut(s) 345, 450
HspAI GCGC 1 cut(s) 345
Kzo9I GATC 5 cut(s) 115, 205, 209, 457, 477
LmnI GCTCC 3 cut(s) 38, 161, 851
Lsp1109I GCAGC 2 cut(s) 379, 533
LweI GCATC 2 cut(s) 65, 115
MaeI CTAG 1 cut(s) 735
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 2 cut(s) 31, 47
MalI GATC 5 cut(s) 117, 207, 211, 459, 479
MboI GATC 5 cut(s) 115, 205, 209, 457, 477
MboII GAAGA 4 cut(s) 215, 375, 485, 620
MflI RGATCY 1 cut(s) 457
MluCI AATT 6 cut(s) 225, 269, 300, 350, 530, 634
MlyI GAGTC 1 cut(s) 922
MnlI CCTC 9 cut(s) 165, 426, 433, 504, 508, 511, 912, 947, 965
MroXI GAANNNNTTC 1 cut(s) 950
MseI TTAA 4 cut(s) 327, 377, 609, 638
MspA1I CMGCKG 1 cut(s) 359
MspI CCGG 2 cut(s) 9, 572
MspR9I CCNGG 3 cut(s) 526, 573, 831
Mva1269I GAATGC 1 cut(s) 674
MvaI CCWGG 2 cut(s) 526, 831
MvnI CGCG 1 cut(s) 114
MwoI GCNNNNNNNGC 4 cut(s) 70, 79, 518, 764
NciI CCSGG 1 cut(s) 573
NdeII GATC 5 cut(s) 115, 205, 209, 457, 477
NlaIII CATG 2 cut(s) 345, 450
NlaIV GGNNCC 4 cut(s) 163, 459, 775, 847
NruI TCGCGA 1 cut(s) 114
NsbI TGCGCA 1 cut(s) 346
PctI GAATGC 1 cut(s) 674
PdmI GAANNNNTTC 1 cut(s) 950
PfeI GAWTC 2 cut(s) 14, 888
PkrI GCNGC 2 cut(s) 394, 523
PleI GAGTC 1 cut(s) 921
PpsI GAGTC 1 cut(s) 921
Psp6I CCWGG 2 cut(s) 524, 829
PspGI CCWGG 2 cut(s) 524, 829
PspN4I GGNNCC 4 cut(s) 163, 459, 775, 847
PspPI GGNCC 2 cut(s) 587, 773
PstI CTGCAG 2 cut(s) 313, 397
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 1 cut(s) 457
RruI TCGCGA 1 cut(s) 114
RsaI GTAC 3 cut(s) 7, 267, 452
RsaNI GTAC 3 cut(s) 6, 266, 451
SaqAI TTAA 4 cut(s) 327, 377, 609, 638
SatI GCNGC 2 cut(s) 393, 522
Sau3AI GATC 5 cut(s) 115, 205, 209, 457, 477
Sau96I GGNCC 2 cut(s) 587, 773
SchI GAGTC 1 cut(s) 922
ScrFI CCNGG 3 cut(s) 526, 573, 831
SfaNI GCATC 2 cut(s) 65, 115
SfcI CTRYAG 2 cut(s) 309, 393
SinI GGWCC 2 cut(s) 587, 773
SmlI CTYRAG 3 cut(s) 917, 938, 956
SmoI CTYRAG 3 cut(s) 917, 938, 956
Sse9I AATT 6 cut(s) 225, 269, 300, 350, 530, 634
SsiI CCGC 2 cut(s) 191, 357
SspMI CTAG 1 cut(s) 735
StyD4I CCNGG 3 cut(s) 524, 571, 829
StyI CCWWGG 2 cut(s) 165, 864
TaaI ACNGT 5 cut(s) 25, 97, 109, 465, 928
TaiI ACGT 1 cut(s) 687
TaqI TCGA 1 cut(s) 208
TasI AATT 6 cut(s) 225, 269, 300, 350, 530, 634
TatI WGTACW 1 cut(s) 265
TfiI GAWTC 2 cut(s) 14, 888
Tru1I TTAA 4 cut(s) 327, 377, 609, 638
Tru9I TTAA 4 cut(s) 327, 377, 609, 638
TscAI CASTG 5 cut(s) 112, 286, 318, 468, 931
TseI GCWGC 2 cut(s) 392, 521
TspDTI ATGAA 3 cut(s) 167, 239, 456
TspGWI ACGGA 2 cut(s) 607, 887
TspRI CASTG 5 cut(s) 112, 286, 318, 468, 931
VpaK11BI GGWCC 2 cut(s) 587, 773
XapI RAATTY 3 cut(s) 350, 530, 634
XmnI GAANNNNTTC 1 cut(s) 950
XspI CTAG 1 cut(s) 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.