RLG00000030523

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000709
Physical Location & Seq
Reverse (-)
62879158 .. 62880886
1729 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030523

Sequence Viewer

Length: 822 bp
ATGAGCGGAGAAAGGAAGGTTGTGTGCGTTACAGGAGCTTCTGGTTTCATAGCTTCATGGTTGGTGAAGCTCTTGTTACAGCGAGGTTATGTCGTCAAAGCCACCGTTCGTGACCCAAATAATCCGAAGAAAATAGAACACTTGCTCTCACTTGAGGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAGGGGTCTTTTGACCTTGCAGTTGATGGATGTGAAGGTGTTTTCCACACAGCATCTCCGGTACTATTTTCATCGATCAGCGACCCCCAGGCAGAATTGATTGATCCTGCAGTAAAGGGAACGCTGAATGTTCTTAAATCGTGCATGAAATTTCCAACAATCAAGAGGGTAGTTTTTACATCTTCCATGGCTGCAGTTATATTCAATGGAAGACCTTTAACCTCTAATGTGGTAGTTGATGAAACATGGTTTTCTGATCCACTTGTTTGTGAGGAGTTGAAGCAATTGATTGTTTGTTTCTTATCCTATACAGATGTCAACTTGACTGATGTTAGAGATGTTGCCTCGGCTCATATTCAAGCATTTGAAGTTCCTTCAGCTACTGGAAGATATTGTTTAGTTGCAAATGTCACCCCCATTCACGAGGCTCGGAAGATTTTAAAGGAAATTCATCCTACTTTGTGCCAACCTGAAATATGTGAGCAGGATATCCCTTCTGCTCGAGAGTATCGAATATCCCAGGAAAAGGCAAAAAGTTTGGGACTTGATTTCCTTCCGTTGGAAGTAAGTCTCAGGGACACTATTGAATGCCTCAAGGAGAAGGGTTTCCTTGAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

274

Amino Acids

30.19

Weight (kDa)

5.61

Isoelectric Point (pI)

32.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 105 2.9e-08 NmrA-like family
Epimerase PF01370 8 - 131 2.5e-14 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 136 8.1e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 129 2.8e-11 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 136 3.4e-08 Male sterility protein
NAD_binding_10 PF13460 12 - 132 2e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 6
AciI CCGC 1 cut(s) 6
AclWI GGATC 2 cut(s) 299, 452
AcsI RAATTY 2 cut(s) 350, 648
AcuI CTGAAG 1 cut(s) 561
AfaI GTAC 1 cut(s) 264
AfiI CCNNNNNNNGG 2 cut(s) 727, 760
AgsI TTSAA 6 cut(s) 184, 406, 481, 560, 569, 788
AjnI CCWGG 2 cut(s) 288, 720
AluBI AGCT 4 cut(s) 38, 53, 70, 581
AluI AGCT 4 cut(s) 38, 53, 70, 581
Alw26I GTCTC 1 cut(s) 776
AlwI GGATC 2 cut(s) 299, 452
AlwNI CAGNNNCTG 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 702
AoxI GGCC 1 cut(s) 816
ApeKI GCWGC 1 cut(s) 392
ApoI RAATTY 2 cut(s) 350, 648
Asp700I GAANNNNTTC 2 cut(s) 171, 806
AsuHPI GGTGA 2 cut(s) 76, 604
AvaI CYCGRG 1 cut(s) 702
BauI CACGAG 1 cut(s) 623
BbsI GAAGAC 1 cut(s) 418
BbvI GCAGC 1 cut(s) 379
BccI CCATC 1 cut(s) 221
BciT130I CCWGG 2 cut(s) 290, 722
BcoDI GTCTC 1 cut(s) 776
BfmI CTRYAG 2 cut(s) 309, 393
BisI GCNGC 1 cut(s) 393
BlsI GCNGC 1 cut(s) 394
Bme1390I CCNGG 2 cut(s) 290, 722
BmeT110I CYCGRG 1 cut(s) 702
BmrFI CCNGG 2 cut(s) 290, 722
BmsI GCATC 1 cut(s) 263
BpiI GAAGAC 1 cut(s) 418
BpuEI CTTGAG 2 cut(s) 173, 779
Bsa29I ATCGAT 1 cut(s) 275
BsaJI CCNNGG 4 cut(s) 288, 387, 546, 720
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 2 cut(s) 727, 760
Bse1I ACTGG 1 cut(s) 589
BseBI CCWGG 2 cut(s) 290, 722
BseCI ATCGAT 1 cut(s) 275
BseDI CCNNGG 4 cut(s) 288, 387, 546, 720
BseGI GGATG 2 cut(s) 236, 652
BseLI CCNNNNNNNGG 2 cut(s) 727, 760
BseMII CTCAG 1 cut(s) 787
BseNI ACTGG 1 cut(s) 589
BseRI GAGGAG 1 cut(s) 488
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 1 cut(s) 818
BshVI ATCGAT 1 cut(s) 275
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 1 cut(s) 260
BslFI GGGAC 2 cut(s) 756, 791
BslI CCNNNNNNNGG 2 cut(s) 727, 760
BsmAI GTCTC 1 cut(s) 776
BsmFI GGGAC 2 cut(s) 756, 791
BsmI GAATGC 1 cut(s) 794
BsnI GGCC 1 cut(s) 818
BsoBI CYCGRG 1 cut(s) 702
Bsp143I GATC 3 cut(s) 276, 304, 457
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 1 cut(s) 6
BspANI GGCC 1 cut(s) 818
BspCNI CTCAG 1 cut(s) 786
BspDI ATCGAT 1 cut(s) 275
BspMAI CTGCAG 2 cut(s) 313, 397
BspPI GGATC 2 cut(s) 299, 452
BsrBI CCGCTC 1 cut(s) 6
BsrI ACTGG 1 cut(s) 589
BssECI CCNNGG 4 cut(s) 288, 387, 546, 720
BssMI GATC 3 cut(s) 276, 304, 457
BssSI CACGAG 1 cut(s) 623
BssT1I CCWWGG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 623
Bst2UI CCWGG 2 cut(s) 290, 722
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 1 cut(s) 195
BstDEI CTNAG 1 cut(s) 773
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 2 cut(s) 236, 652
BstKTI GATC 3 cut(s) 279, 307, 460
BstMAI GTCTC 1 cut(s) 776
BstMBI GATC 3 cut(s) 276, 304, 457
BstNI CCWGG 2 cut(s) 290, 722
BstSCI CCNGG 2 cut(s) 288, 720
BstSFI CTRYAG 2 cut(s) 309, 393
BstV1I GCAGC 1 cut(s) 379
BstV2I GAAGAC 1 cut(s) 418
Bsu15I ATCGAT 1 cut(s) 275
BsuRI GGCC 1 cut(s) 818
BsuTUI ATCGAT 1 cut(s) 275
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 2 cut(s) 236, 652
CaiI CAGNNNCTG 1 cut(s) 584
ClaI ATCGAT 1 cut(s) 275
Csp6I GTAC 1 cut(s) 263
CviAII CATG 4 cut(s) 57, 346, 388, 447
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 1 cut(s) 773
DpnI GATC 3 cut(s) 278, 306, 459
DpnII GATC 3 cut(s) 276, 304, 457
DraI TTTAAA 1 cut(s) 642
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 387
Eco147I AGGCCT 1 cut(s) 818
Eco32I GATATC 1 cut(s) 691
Eco57I CTGAAG 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 702
EcoRII CCWGG 2 cut(s) 288, 720
EcoRV GATATC 1 cut(s) 691
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 4 cut(s) 60, 349, 391, 450
FaqI GGGAC 2 cut(s) 756, 791
FatI CATG 4 cut(s) 56, 345, 387, 446
Fnu4HI GCNGC 1 cut(s) 393
FokI GGATG 2 cut(s) 243, 639
Fsp4HI GCNGC 1 cut(s) 393
GluI GCNGC 1 cut(s) 393
HaeIII GGCC 1 cut(s) 818
HapII CCGG 1 cut(s) 260
Hin1II CATG 4 cut(s) 60, 349, 391, 450
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HpaII CCGG 1 cut(s) 260
HphI GGTGA 2 cut(s) 76, 604
Hpy166II GTNNAC 1 cut(s) 520
Hpy188I TCNGA 3 cut(s) 126, 457, 633
Hpy188III TCNNGA 4 cut(s) 110, 364, 623, 704
Hpy8I GTNNAC 1 cut(s) 520
HpyAV CCTTC 6 cut(s) 10, 230, 585, 705, 764, 796
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4V TGCA 5 cut(s) 221, 311, 345, 395, 605
HpyF3I CTNAG 1 cut(s) 773
Hsp92II CATG 4 cut(s) 60, 349, 391, 450
Kzo9I GATC 3 cut(s) 276, 304, 457
LmnI GCTCC 2 cut(s) 35, 158
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 1 cut(s) 263
MaeIII GTNAC 4 cut(s) 28, 75, 110, 610
MalI GATC 3 cut(s) 278, 306, 459
MbiI CCGCTC 1 cut(s) 6
MboI GATC 3 cut(s) 276, 304, 457
MboII GAAGA 6 cut(s) 139, 212, 375, 423, 600, 646
MfeI CAATTG 1 cut(s) 485
MluCI AATT 4 cut(s) 296, 350, 485, 648
MmeI TCCRAC 2 cut(s) 380, 741
MroXI GAANNNNTTC 2 cut(s) 171, 806
MseI TTAA 3 cut(s) 336, 419, 641
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 2 cut(s) 290, 722
MunI CAATTG 1 cut(s) 485
Mva1269I GAATGC 1 cut(s) 794
MvaI CCWGG 2 cut(s) 290, 722
NcoI CCATGG 1 cut(s) 387
NdeII GATC 3 cut(s) 276, 304, 457
NlaIII CATG 4 cut(s) 60, 349, 391, 450
NmeAIII GCCGAG 1 cut(s) 527
NmuCI GTSAC 2 cut(s) 110, 610
PaeR7I CTCGAG 1 cut(s) 702
PceI AGGCCT 1 cut(s) 818
PcsI WCGNNNNNNNCGW 1 cut(s) 709
PctI GAATGC 1 cut(s) 794
PdmI GAANNNNTTC 2 cut(s) 171, 806
PkrI GCNGC 1 cut(s) 394
Psp6I CCWGG 2 cut(s) 288, 720
PspGI CCWGG 2 cut(s) 288, 720
PstI CTGCAG 2 cut(s) 313, 397
PstNI CAGNNNCTG 1 cut(s) 584
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
SaqAI TTAA 3 cut(s) 336, 419, 641
SatI GCNGC 1 cut(s) 393
Sau3AI GATC 3 cut(s) 276, 304, 457
ScrFI CCNGG 2 cut(s) 290, 722
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 2 cut(s) 309, 393
Sfr274I CTCGAG 1 cut(s) 702
SlaI CTCGAG 1 cut(s) 702
SmlI CTYRAG 4 cut(s) 152, 702, 794, 812
SmoI CTYRAG 4 cut(s) 152, 702, 794, 812
Sse9I AATT 4 cut(s) 296, 350, 485, 648
SseBI AGGCCT 1 cut(s) 818
SsiI CCGC 1 cut(s) 6
StuI AGGCCT 1 cut(s) 818
StyD4I CCNGG 2 cut(s) 288, 720
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 106
TaqI TCGA 3 cut(s) 275, 703, 712
TasI AATT 4 cut(s) 296, 350, 485, 648
Tru1I TTAA 3 cut(s) 336, 419, 641
Tru9I TTAA 3 cut(s) 336, 419, 641
TseFI GTSAC 2 cut(s) 110, 610
TseI GCWGC 1 cut(s) 392
Tsp45I GTSAC 2 cut(s) 110, 610
TspDTI ATGAA 7 cut(s) 37, 45, 164, 261, 362, 456, 641
TspGWI ACGGA 1 cut(s) 747
XapI RAATTY 2 cut(s) 350, 648
XhoI CTCGAG 1 cut(s) 702
XmnI GAANNNNTTC 2 cut(s) 171, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.