Prupe.2G021100_v2.0.a1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000709
Physical Location & Seq
Reverse (-)
2073208 .. 2074625
1418 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G021100.1

Sequence Viewer

Length: 855 bp
GACTCAAAGAAAACAGAACACTTACTGGCACTTGATGGGGCAAAAGAAAGGCTGCATTTGTTCCAAGCTGATTTGATAGAAGAAGGATCTTTTGACGCTGCAGTTGATGGATGTGAAGGTGTTTTTCATACAGCATCCCCTGTTCTATTTTCAGCCACTAACCCTCAGGCAGAATTAATTGACCCTGCAGTGAAGGGAACCCTTAATGTTCTAAAATCGTGCGTAAAATTCGCTACCGTCAAGAGGGTAGTTTTAACATCTTCTATGGCTGCAGTCATTTTCAATGGAAGACCATTGACCCCTGATGTGGTGATTGATGAAACATGGTTTTCAGATCCTCTTGTTTGTGAGAATTTGAAGGAATGGTATTTTCTTTCAAAAACTTTAGCTGAGGAGGCTGCTTGGAAATTTGCTAAAGGAAATGGGATTGACTTGGTTACCATAAATCCATCTTATGTGATTGGTCCACTCTTACAACCAAGTCTTAATGCCACTGTGGAAATGGTTCTGAATCTCAAAAATGATATACCAGATGTAACCCGTTCAAATTATTTGTCTACTGATGTTAGAGATGTTGCCTCTGCTCATGTTCAAGCATTTGAGGTTCCTTCAGCTACTGGAAGATATTGTTTAGTTGGACATGTTATACCCATGTCCAAGGCTCTCAAGATTTTACATGAACTTCATCCCACTCTTTTTCCACCTGAAAAATATGAGGATGGCAATCCTTCAGAACCCACATATCAAGTATCACAGGAAAAAGCAAAAAGTTTGGGAGTTAATTTCCTTCCGGTGGAAGTAAGTCTTAGGGACACTATTGAAAGCCTCAAGGAGAAGGGCTTCCTCAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

285

Amino Acids

31.17

Weight (kDa)

5.22

Isoelectric Point (pI)

28.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 557
AclWI GGATC 2 cut(s) 94, 329
AcsI RAATTY 3 cut(s) 227, 352, 407
AcuI CTGAAG 2 cut(s) 594, 714
AfiI CCNNNNNNNGG 3 cut(s) 243, 307, 793
AflIII ACRYGT 1 cut(s) 640
AgsI TTSAA 6 cut(s) 283, 358, 378, 546, 593, 821
AluBI AGCT 3 cut(s) 68, 389, 614
AluI AGCT 3 cut(s) 68, 389, 614
AlwI GGATC 2 cut(s) 94, 329
AlwNI CAGNNNCTG 1 cut(s) 617
ApeKI GCWGC 4 cut(s) 52, 98, 269, 398
ApoI RAATTY 3 cut(s) 227, 352, 407
ArsI GACNNNNNNTTYG 2 cut(s) 539, 571
AseI ATTAAT 1 cut(s) 176
Asp700I GAANNNNTTC 2 cut(s) 504, 839
AspS9I GGNCC 1 cut(s) 464
AsuHPI GGTGA 1 cut(s) 322
AvaII GGWCC 1 cut(s) 464
AxyI CCTNAGG 1 cut(s) 165
BbsI GAAGAC 1 cut(s) 295
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 4 cut(s) 39, 85, 256, 385
BccI CCATC 4 cut(s) 29, 101, 457, 713
BfmI CTRYAG 3 cut(s) 99, 186, 270
BisI GCNGC 4 cut(s) 53, 99, 270, 399
BlsI GCNGC 4 cut(s) 54, 100, 271, 400
Bme18I GGWCC 1 cut(s) 464
BmgT120I GGNCC 1 cut(s) 464
BmiI GGNNCC 2 cut(s) 199, 606
BmsI GCATC 1 cut(s) 143
BpiI GAAGAC 1 cut(s) 295
Bpu10I CCTNAGC 1 cut(s) 390
BpuEI CTTGAG 3 cut(s) 650, 812, 830
BsaBI GATNNNNATC 1 cut(s) 723
BsaJI CCNNGG 1 cut(s) 657
BsaWI WCCGGW 1 cut(s) 790
Bsc4I CCNNNNNNNGG 3 cut(s) 243, 307, 793
Bse1I ACTGG 2 cut(s) 30, 622
Bse21I CCTNAGG 1 cut(s) 165
Bse8I GATNNNNATC 1 cut(s) 723
BseDI CCNNGG 1 cut(s) 657
BseGI GGATG 4 cut(s) 116, 134, 685, 724
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 3 cut(s) 243, 307, 793
BseMII CTCAG 2 cut(s) 179, 381
BseNI ACTGG 2 cut(s) 30, 622
BseRI GAGGAG 1 cut(s) 407
BseXI GCAGC 4 cut(s) 39, 85, 256, 385
BsiSI CCGG 1 cut(s) 791
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 3 cut(s) 243, 307, 793
BsmFI GGGAC 1 cut(s) 824
Bsp143I GATC 2 cut(s) 86, 334
BspCNI CTCAG 2 cut(s) 178, 382
BspLI GGNNCC 2 cut(s) 199, 606
BspMAI CTGCAG 3 cut(s) 103, 190, 274
BspPI GGATC 2 cut(s) 94, 329
BsrI ACTGG 2 cut(s) 30, 622
BssECI CCNNGG 1 cut(s) 657
BssMI GATC 2 cut(s) 86, 334
BssT1I CCWWGG 1 cut(s) 657
Bst4CI ACNGT 2 cut(s) 238, 496
BstDEI CTNAG 3 cut(s) 165, 390, 806
BstEII GGTNACC 1 cut(s) 436
BstF5I GGATG 4 cut(s) 116, 134, 685, 724
BstKTI GATC 2 cut(s) 89, 337
BstMBI GATC 2 cut(s) 86, 334
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNSI RCATGY 1 cut(s) 644
BstPI GGTNACC 1 cut(s) 436
BstSFI CTRYAG 3 cut(s) 99, 186, 270
BstV1I GCAGC 4 cut(s) 39, 85, 256, 385
BstV2I GAAGAC 1 cut(s) 295
BstX2I RGATCY 2 cut(s) 86, 334
BstYI RGATCY 2 cut(s) 86, 334
Bsu36I CCTNAGG 1 cut(s) 165
BtsCI GGATG 4 cut(s) 116, 134, 685, 724
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 2 cut(s) 195, 492
CaiI CAGNNNCTG 1 cut(s) 617
Cfr13I GGNCC 1 cut(s) 464
CseI GACGC 1 cut(s) 104
CviAII CATG 5 cut(s) 324, 587, 641, 652, 677
DdeI CTNAG 3 cut(s) 165, 390, 806
DpnI GATC 2 cut(s) 88, 336
DpnII GATC 2 cut(s) 86, 334
Eco130I CCWWGG 1 cut(s) 657
Eco47I GGWCC 1 cut(s) 464
Eco57I CTGAAG 2 cut(s) 594, 714
Eco81I CCTNAGG 1 cut(s) 165
Eco91I GGTNACC 1 cut(s) 436
EcoO65I GGTNACC 1 cut(s) 436
EcoT14I CCWWGG 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 5 cut(s) 327, 590, 644, 655, 680
FalI AAGNNNNNCTT 2 cut(s) 789, 821
FaqI GGGAC 1 cut(s) 824
FatI CATG 5 cut(s) 323, 586, 640, 651, 676
FblI GTMKAC 1 cut(s) 557
Fnu4HI GCNGC 4 cut(s) 53, 99, 270, 399
FokI GGATG 4 cut(s) 121, 123, 672, 731
Fsp4HI GCNGC 4 cut(s) 53, 99, 270, 399
GluI GCNGC 4 cut(s) 53, 99, 270, 399
HapII CCGG 1 cut(s) 791
HgaI GACGC 1 cut(s) 104
Hin1II CATG 5 cut(s) 327, 590, 644, 655, 680
HinfI GANTC 2 cut(s) 2, 511
HpaII CCGG 1 cut(s) 791
HphI GGTGA 1 cut(s) 322
Hpy166II GTNNAC 2 cut(s) 467, 558
Hpy188I TCNGA 3 cut(s) 334, 510, 733
Hpy188III TCNNGA 2 cut(s) 241, 667
Hpy8I GTNNAC 2 cut(s) 467, 558
HpyAV CCTTC 8 cut(s) 77, 110, 187, 352, 618, 738, 797, 829
HpyCH4III ACNGT 2 cut(s) 238, 496
HpyCH4V TGCA 4 cut(s) 55, 101, 188, 272
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 3 cut(s) 165, 390, 806
Hsp92II CATG 5 cut(s) 327, 590, 644, 655, 680
Kzo9I GATC 2 cut(s) 86, 334
Lsp1109I GCAGC 4 cut(s) 39, 85, 256, 385
LweI GCATC 1 cut(s) 143
MaeIII GTNAC 2 cut(s) 436, 535
MalI GATC 2 cut(s) 88, 336
MboI GATC 2 cut(s) 86, 334
MboII GAAGA 4 cut(s) 92, 252, 300, 633
MflI RGATCY 2 cut(s) 86, 334
MluCI AATT 7 cut(s) 173, 177, 227, 352, 407, 547, 781
MmeI TCCRAC 1 cut(s) 616
MroXI GAANNNNTTC 2 cut(s) 504, 839
MseI TTAA 5 cut(s) 176, 204, 254, 486, 780
MspI CCGG 1 cut(s) 791
MwoI GCNNNNNNNGC 1 cut(s) 395
NdeII GATC 2 cut(s) 86, 334
NlaIII CATG 5 cut(s) 327, 590, 644, 655, 680
NlaIV GGNNCC 2 cut(s) 199, 606
NspI RCATGY 1 cut(s) 644
PciI ACATGT 1 cut(s) 640
PdmI GAANNNNTTC 2 cut(s) 504, 839
PfeI GAWTC 1 cut(s) 511
PkrI GCNGC 4 cut(s) 54, 100, 271, 400
PscI ACATGT 1 cut(s) 640
PshBI ATTAAT 1 cut(s) 176
PspEI GGTNACC 1 cut(s) 436
PspN4I GGNNCC 2 cut(s) 199, 606
PspPI GGNCC 1 cut(s) 464
PstI CTGCAG 3 cut(s) 103, 190, 274
PstNI CAGNNNCTG 1 cut(s) 617
PsuI RGATCY 2 cut(s) 86, 334
SaqAI TTAA 5 cut(s) 176, 204, 254, 486, 780
SatI GCNGC 4 cut(s) 53, 99, 270, 399
Sau3AI GATC 2 cut(s) 86, 334
Sau96I GGNCC 1 cut(s) 464
SetI ASST 7 cut(s) 70, 121, 391, 606, 616, 706, 852
SfaNI GCATC 1 cut(s) 143
SfcI CTRYAG 3 cut(s) 99, 186, 270
SinI GGWCC 1 cut(s) 464
SmlI CTYRAG 3 cut(s) 665, 827, 845
SmoI CTYRAG 3 cut(s) 665, 827, 845
Sse9I AATT 7 cut(s) 173, 177, 227, 352, 407, 547, 781
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 2 cut(s) 238, 496
TasI AATT 7 cut(s) 173, 177, 227, 352, 407, 547, 781
TfiI GAWTC 1 cut(s) 511
Tru1I TTAA 5 cut(s) 176, 204, 254, 486, 780
Tru9I TTAA 5 cut(s) 176, 204, 254, 486, 780
TscAI CASTG 2 cut(s) 195, 499
TseI GCWGC 4 cut(s) 52, 98, 269, 398
TspDTI ATGAA 4 cut(s) 116, 333, 674, 693
TspRI CASTG 2 cut(s) 195, 499
VpaK11BI GGWCC 1 cut(s) 464
VspI ATTAAT 1 cut(s) 176
XapI RAATTY 3 cut(s) 227, 352, 407
XceI RCATGY 1 cut(s) 644
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 557
XmnI GAANNNNTTC 2 cut(s) 504, 839
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.