MD07G1021500.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000709
Physical Location & Seq
Reverse (-)
1818742 .. 1821463
2722 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1021500.v1.1.491

Sequence Viewer

Length: 951 bp
ATGAAAAGCACAGTACTGAACGAAATTAAGCTTATTAATTCTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATAGGATGAGTACCGGAGAATCAAGGACTGTATGTGTAACAGGAGCCTCTGGTTACATAGCATCATGGCTGGTGAAGCTCTTACTGCAAAAGGGTTATAATGTCAAAGCCACTGTTCGTGATCCAATTGATGGATGTGTAGGTGTTTTTCATACAGCATCGCCTGTACAATATTCAGCCACTGACCCACAGGTAGAAATAATTGATCCTGCAGTGAAAGGAACACTTAATGTTTTAAAATCATGCGCAAAATTCCCCGCTGTCAAGAGAGTGGTTTTAACATCTTCTATGGCTTCGGTAGGGATTAGTGGAAAACCTCTGACCTCTGATGTGGTTATGGATGAAACATGGTACTCGGATCCATTTTTTTGTGAGAAGACCGAGCAATGGTATCCTCTCTCAAAAACTTTAGCTGAGGAGGCCGCCTGGAAATTTGCTAAAGGAAATGGGATTGACTTGGTATCAATACATCCAGGGATTGTCATTGGTCCACTCTTACAGCCAACTCTTAATCTGTCCATCGAGTATTTTCTGGATTTAATGAGTGGTATCAAAACACTGTTTATAAACCACACATTCGTGGACGTTAGAGATGTTGCCTCTGCTCATATTCAAGCATTTGAAGCTCCTTCAGCTAGTGGCAGATATTGTTTAGTTGCCCAAGTTGCTGACAGTCCCGATACTCTAAAGATTTTACGAGAGCTTTACCCCACATTGAGCCTTCCTGAGCTTGGCAATCCTTCTGACTCGAAGTTTCAAGTGTCCAGGGAGAAAGCAAAAGGTTTGGGAATCACTTTCCTTCCTCTGGAAACAAGTCTCAAGGACACTGTTGAAAGTCTCAAGGAGAAGGGCTTCCTCAAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

317

Amino Acids

34.95

Weight (kDa)

6.47

Isoelectric Point (pI)

27.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 64 - 235 5.1e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 76 - 241 3.2e-10 NAD dependent epimerase/dehydratase family
NAD_binding_4 PF07993 76 - 195 3.4e-06 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 183, 650
Acc16I TGCGCA 1 cut(s) 331
AccB7I CCANNNNNTGG 1 cut(s) 215
AciI CCGC 2 cut(s) 342, 507
AclWI GGATC 4 cut(s) 200, 284, 437, 450
AcsI RAATTY 2 cut(s) 335, 515
AcuI CTGAAG 1 cut(s) 699
AfaI GTAC 4 cut(s) 15, 97, 252, 437
AfiI CCNNNNNNNGG 4 cut(s) 215, 471, 815, 889
AgsI TTSAA 4 cut(s) 698, 707, 842, 917
AjnI CCWGG 3 cut(s) 509, 556, 848
AleI CACNNNNGTG 1 cut(s) 662
AluBI AGCT 7 cut(s) 31, 163, 497, 710, 719, 787, 814
AluI AGCT 7 cut(s) 31, 163, 497, 710, 719, 787, 814
Alw26I GTCTC 2 cut(s) 905, 926
AlwI GGATC 4 cut(s) 200, 284, 437, 450
AlwNI CAGNNNCTG 1 cut(s) 266
AoxI GGCC 1 cut(s) 504
ApoI RAATTY 2 cut(s) 335, 515
AseI ATTAAT 1 cut(s) 36
Asp700I GAANNNNTTC 1 cut(s) 935
AspLEI GCGC 1 cut(s) 332
AspS9I GGNCC 1 cut(s) 572
AsuHPI GGTGA 1 cut(s) 169
AvaII GGWCC 1 cut(s) 572
BamHI GGATCC 1 cut(s) 442
BbsI GAAGAC 1 cut(s) 467
BbvCI CCTCAGC 1 cut(s) 498
BccI CCATC 2 cut(s) 209, 611
BciT130I CCWGG 3 cut(s) 511, 558, 850
BciVI GTATCC 1 cut(s) 486
BcoDI GTCTC 2 cut(s) 905, 926
BfaI CTAG 1 cut(s) 720
BfmI CTRYAG 1 cut(s) 294
BfuI GTATCC 1 cut(s) 486
BisI GCNGC 1 cut(s) 507
BlsI GCNGC 1 cut(s) 508
BmcAI AGTACT 1 cut(s) 15
Bme1390I CCNGG 3 cut(s) 511, 558, 850
Bme18I GGWCC 1 cut(s) 572
BmgT120I GGNCC 1 cut(s) 572
BmiI GGNNCC 2 cut(s) 130, 444
BmrFI CCNGG 3 cut(s) 511, 558, 850
BmsI GCATC 2 cut(s) 155, 251
BpiI GAAGAC 1 cut(s) 467
Bpu10I CCTNAGC 2 cut(s) 498, 810
BpuEI CTTGAG 3 cut(s) 887, 908, 926
BsaJI CCNNGG 2 cut(s) 557, 849
BsaWI WCCGGW 1 cut(s) 98
Bsc4I CCNNNNNNNGG 4 cut(s) 215, 471, 815, 889
Bse3DI GCAATG 1 cut(s) 476
BseBI CCWGG 3 cut(s) 511, 558, 850
BseDI CCNNGG 2 cut(s) 557, 849
BseGI GGATG 4 cut(s) 96, 224, 430, 553
BseLI CCNNNNNNNGG 4 cut(s) 215, 471, 815, 889
BseMI GCAATG 1 cut(s) 476
BseMII CTCAG 3 cut(s) 33, 489, 801
BseRI GAGGAG 1 cut(s) 515
BshFI GGCC 1 cut(s) 506
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 744
BslI CCNNNNNNNGG 4 cut(s) 215, 471, 815, 889
BsmAI GTCTC 2 cut(s) 905, 926
BsmFI GGGAC 1 cut(s) 744
BsnI GGCC 1 cut(s) 506
Bsp1407I TGTACA 1 cut(s) 250
Bsp143I GATC 3 cut(s) 205, 289, 442
BspACI CCGC 2 cut(s) 342, 507
BspANI GGCC 1 cut(s) 506
BspCNI CTCAG 3 cut(s) 34, 490, 802
BspLI GGNNCC 2 cut(s) 130, 444
BspMAI CTGCAG 1 cut(s) 298
BspPI GGATC 4 cut(s) 200, 284, 437, 450
BsrDI GCAATG 1 cut(s) 476
BsrGI TGTACA 1 cut(s) 250
BssECI CCNNGG 2 cut(s) 557, 849
BssMI GATC 3 cut(s) 205, 289, 442
Bst2UI CCWGG 3 cut(s) 511, 558, 850
Bst4CI ACNGT 6 cut(s) 13, 115, 199, 645, 758, 913
BstAUI TGTACA 1 cut(s) 250
BstDEI CTNAG 3 cut(s) 42, 498, 810
BstF5I GGATG 4 cut(s) 96, 224, 430, 553
BstHHI GCGC 1 cut(s) 332
BstKTI GATC 3 cut(s) 208, 292, 445
BstMAI GTCTC 2 cut(s) 905, 926
BstMBI GATC 3 cut(s) 205, 289, 442
BstMWI GCNNNNNNNGC 6 cut(s) 160, 169, 503, 707, 716, 749
BstNI CCWGG 3 cut(s) 511, 558, 850
BstSCI CCNGG 3 cut(s) 509, 556, 848
BstSFI CTRYAG 1 cut(s) 294
BstV2I GAAGAC 1 cut(s) 467
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BsuI GTATCC 1 cut(s) 486
BsuRI GGCC 1 cut(s) 506
BtgZI GCGATG 1 cut(s) 228
BtsCI GGATG 4 cut(s) 96, 224, 430, 553
BtsI GCAGTG 1 cut(s) 303
BtsIMutI CAGTG 5 cut(s) 195, 264, 303, 641, 909
CaiI CAGNNNCTG 1 cut(s) 266
CfoI GCGC 1 cut(s) 332
Cfr13I GGNCC 1 cut(s) 572
Csp6I GTAC 4 cut(s) 14, 96, 251, 436
CviAII CATG 3 cut(s) 150, 327, 432
CviQI GTAC 4 cut(s) 14, 96, 251, 436
DdeI CTNAG 3 cut(s) 42, 498, 810
DpnI GATC 3 cut(s) 207, 291, 444
DpnII GATC 3 cut(s) 205, 289, 442
DraI TTTAAA 1 cut(s) 321
Eco47I GGWCC 1 cut(s) 572
Eco57I CTGAAG 1 cut(s) 699
EcoRII CCWGG 3 cut(s) 509, 556, 848
FaeI CATG 3 cut(s) 153, 330, 435
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FaqI GGGAC 1 cut(s) 744
FatI CATG 3 cut(s) 149, 326, 431
FauI CCCGC 1 cut(s) 349
Fnu4HI GCNGC 1 cut(s) 507
FokI GGATG 4 cut(s) 103, 231, 437, 540
Fsp4HI GCNGC 1 cut(s) 507
FspBI CTAG 1 cut(s) 720
FspI TGCGCA 1 cut(s) 331
GlaI GCGC 1 cut(s) 331
GluI GCNGC 1 cut(s) 507
HaeIII GGCC 1 cut(s) 506
HapII CCGG 1 cut(s) 99
HhaI GCGC 1 cut(s) 332
Hin1II CATG 3 cut(s) 153, 330, 435
Hin6I GCGC 1 cut(s) 330
HinP1I GCGC 1 cut(s) 330
HindIII AAGCTT 1 cut(s) 29
HinfI GANTC 3 cut(s) 104, 830, 873
HpaII CCGG 1 cut(s) 99
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 2 cut(s) 575, 667
Hpy188I TCNGA 5 cut(s) 43, 405, 412, 442, 829
Hpy188III TCNNGA 6 cut(s) 203, 349, 617, 761, 809, 890
Hpy8I GTNNAC 2 cut(s) 575, 667
HpyAV CCTTC 5 cut(s) 723, 815, 834, 893, 925
HpyCH4III ACNGT 6 cut(s) 13, 115, 199, 645, 758, 913
HpyCH4IV ACGT 1 cut(s) 669
HpyCH4V TGCA 2 cut(s) 172, 296
HpyF10VI GCNNNNNNNGC 6 cut(s) 160, 169, 503, 707, 716, 749
HpyF3I CTNAG 3 cut(s) 42, 498, 810
HpySE526I ACGT 1 cut(s) 669
Hsp92II CATG 3 cut(s) 153, 330, 435
HspAI GCGC 1 cut(s) 330
Kzo9I GATC 3 cut(s) 205, 289, 442
LmnI GCTCC 2 cut(s) 128, 715
LweI GCATC 2 cut(s) 155, 251
MaeI CTAG 1 cut(s) 720
MaeII ACGT 1 cut(s) 669
MaeIII GTNAC 2 cut(s) 121, 137
MalI GATC 3 cut(s) 207, 291, 444
MboI GATC 3 cut(s) 205, 289, 442
MboII GAAGA 2 cut(s) 360, 472
MfeI CAATTG 1 cut(s) 210
MflI RGATCY 1 cut(s) 442
MluCI AATT 6 cut(s) 24, 37, 210, 285, 335, 515
MlyI GAGTC 1 cut(s) 824
MnlI CCTC 9 cut(s) 142, 411, 418, 489, 493, 496, 694, 897, 950
MroXI GAANNNNTTC 1 cut(s) 935
MseI TTAA 7 cut(s) 27, 36, 312, 320, 362, 594, 623
MslI CAYNNNNRTG 1 cut(s) 662
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 3 cut(s) 511, 558, 850
MunI CAATTG 1 cut(s) 210
MvaI CCWGG 3 cut(s) 511, 558, 850
MwoI GCNNNNNNNGC 6 cut(s) 160, 169, 503, 707, 716, 749
NdeII GATC 3 cut(s) 205, 289, 442
NlaIII CATG 3 cut(s) 153, 330, 435
NlaIV GGNNCC 2 cut(s) 130, 444
NsbI TGCGCA 1 cut(s) 331
OliI CACNNNNGTG 1 cut(s) 662
PdmI GAANNNNTTC 1 cut(s) 935
PfeI GAWTC 2 cut(s) 104, 873
PflMI CCANNNNNTGG 1 cut(s) 215
PkrI GCNGC 1 cut(s) 508
PleI GAGTC 1 cut(s) 824
PpsI GAGTC 1 cut(s) 824
PshBI ATTAAT 1 cut(s) 36
PsiI TTATAA 2 cut(s) 183, 650
Psp6I CCWGG 3 cut(s) 509, 556, 848
PspGI CCWGG 3 cut(s) 509, 556, 848
PspN4I GGNNCC 2 cut(s) 130, 444
PspPI GGNCC 1 cut(s) 572
PstI CTGCAG 1 cut(s) 298
PstNI CAGNNNCTG 1 cut(s) 266
PsuI RGATCY 1 cut(s) 442
RsaI GTAC 4 cut(s) 15, 97, 252, 437
RsaNI GTAC 4 cut(s) 14, 96, 251, 436
RseI CAYNNNNRTG 1 cut(s) 662
SaqAI TTAA 7 cut(s) 27, 36, 312, 320, 362, 594, 623
SatI GCNGC 1 cut(s) 507
Sau3AI GATC 3 cut(s) 205, 289, 442
Sau96I GGNCC 1 cut(s) 572
ScaI AGTACT 1 cut(s) 15
SchI GAGTC 1 cut(s) 824
ScrFI CCNGG 3 cut(s) 511, 558, 850
SfaNI GCATC 2 cut(s) 155, 251
SfcI CTRYAG 1 cut(s) 294
SinI GGWCC 1 cut(s) 572
SmiMI CAYNNNNRTG 1 cut(s) 662
SmlI CTYRAG 3 cut(s) 902, 923, 941
SmoI CTYRAG 3 cut(s) 902, 923, 941
Sse9I AATT 6 cut(s) 24, 37, 210, 285, 335, 515
SsiI CCGC 2 cut(s) 342, 507
SspI AATATT 1 cut(s) 257
SspMI CTAG 1 cut(s) 720
StyD4I CCNGG 3 cut(s) 509, 556, 848
TaaI ACNGT 6 cut(s) 13, 115, 199, 645, 758, 913
TaiI ACGT 1 cut(s) 672
TaqI TCGA 2 cut(s) 606, 833
TaqII GACCGA 1 cut(s) 479
TasI AATT 6 cut(s) 24, 37, 210, 285, 335, 515
TatI WGTACW 2 cut(s) 13, 250
TauI GCSGC 1 cut(s) 509
TfiI GAWTC 2 cut(s) 104, 873
Tru1I TTAA 7 cut(s) 27, 36, 312, 320, 362, 594, 623
Tru9I TTAA 7 cut(s) 27, 36, 312, 320, 362, 594, 623
TscAI CASTG 5 cut(s) 202, 271, 303, 648, 916
TspDTI ATGAA 3 cut(s) 17, 224, 441
TspRI CASTG 5 cut(s) 202, 271, 303, 648, 916
Van91I CCANNNNNTGG 1 cut(s) 215
VpaK11BI GGWCC 1 cut(s) 572
VspI ATTAAT 1 cut(s) 36
XapI RAATTY 2 cut(s) 335, 515
XmnI GAANNNNTTC 1 cut(s) 935
XspI CTAG 1 cut(s) 720
ZrmI AGTACT 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.