pycom02g25550

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000709
Physical Location & Seq
Forward (+)
23420877 .. 23422787
1911 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25550.2

Sequence Viewer

Length: 1053 bp
ATGAGTGCCAAAATGATCATCCCATTAAAAACCAACAATCAAGCTGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATGAGTACTGTAGAAGGGAAGACTGTGTGCGTAACAGGAGCCTCAGGTTACATAGCATCATGGTTGGTGAAGCTCTTATTGCAAAAGGGTTATACTGTCAAAGGCACCGTTCGAGACCCAAATGATTCAAAGAAAACTGAACACTTGCGCTCATTAGACGGAGCCAAAGAAAGGCTTCATTTGTTCAAAGCAGATGTATCAGAAGAAGGGTCTTTCGACGCCATAGTTGATGGATGCCAAGGTGTTTTTCATACAGCGTCCCCTGTACTGTTTTCTGTTACTGACCCTCAGGGAGAACTACTTGACCCTGCAGTGAAGGGAACACTTAATGTTCTACAATCATGTGCGAAATTTCGTAGCATCACGAGAGTGGTTTTAACATCTTCACTTGCTTCAGTAATGATGACTGGAGCCCCTCTGACCTCTGATGTGGTTTTGGATGAAACATGGTACTCGGATCCACTTTTTTGTGAGAAGCACAAGCAATGGTATTTGCTCTCAAAAACTTTAGCAGAGGAGACTGCCTGGAAATTTGCTAAAGGAAATGGGATTGACTTGGTGTCGTTGAATCCAGGGTTAACCATTGGTCCACTCTTACAACCAACACTTAATTTCTCCGTCGAGCTGCTTCAGAACTTCATGAGTGGTACCCAAACAACTATCAGTAATCCATTCGTTGACGTTAGAGATGTTGCCTCTGCTCATATTCAAGCTTTCGAAATTCCTTCAGCTAGTGGGAGATATTGTTTAGTTGGCCAAGTTGCTGACGACCTTGATATTCTAAAGATTTTACGGCAACTTTACCCGACTTTGACCCTTGCTGAAGTTGTCAATCCTTCAGACTCTAAGTATCAAGTGTCCAAGGAGAAAGCAAAAGGTTTGGGAATCACTTTCCTTCCTCTGGAAACAAGTCTTAGGGACACTATTGAAAGCCTCGAGGAGAAGGGGTTCCTCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

351

Amino Acids

38.69

Weight (kDa)

5.71

Isoelectric Point (pI)

29.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 1025
Acc65I GGTACC 1 cut(s) 737
AccB1I GGYRCC 2 cut(s) 194, 737
AclWI GGATC 2 cut(s) 542, 555
AcoI YGGCCR 1 cut(s) 844
AcsI RAATTY 3 cut(s) 440, 620, 810
AcuI CTGAAG 5 cut(s) 468, 704, 801, 912, 933
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 4 cut(s) 97, 357, 542, 739
AfiI CCNNNNNNNGG 2 cut(s) 261, 991
AgsI TTSAA 5 cut(s) 219, 277, 658, 800, 1019
AhdI GACNNNNNGTC 1 cut(s) 649
AjnI CCWGG 2 cut(s) 614, 661
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AleI CACNNNNGTG 1 cut(s) 458
AloI GAACNNNNNNTCC 1 cut(s) 1022
AluBI AGCT 5 cut(s) 44, 163, 715, 803, 821
AluI AGCT 5 cut(s) 44, 163, 715, 803, 821
Alw26I GTCTC 2 cut(s) 198, 602
AlwI GGATC 2 cut(s) 542, 555
Ama87I CYCGRG 1 cut(s) 1025
AoxI GGCC 1 cut(s) 844
ApeKI GCWGC 1 cut(s) 715
ApoI RAATTY 3 cut(s) 440, 620, 810
Asp700I GAANNNNTTC 2 cut(s) 264, 1037
Asp718I GGTACC 1 cut(s) 737
AspLEI GCGC 1 cut(s) 240
AspS9I GGNCC 1 cut(s) 677
AsuHPI GGTGA 1 cut(s) 169
AsuII TTCGAA 1 cut(s) 807
AvaI CYCGRG 1 cut(s) 1025
AvaII GGWCC 1 cut(s) 677
AxyI CCTNAGG 2 cut(s) 133, 378
BalI TGGCCA 1 cut(s) 846
BamHI GGATCC 1 cut(s) 547
BanI GGYRCC 2 cut(s) 194, 737
BanII GRGCYC 1 cut(s) 505
BauI CACGAG 1 cut(s) 454
BbsI GAAGAC 1 cut(s) 116
BbvI GCAGC 1 cut(s) 702
BccI CCATC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 899
BciT130I CCWGG 2 cut(s) 616, 663
BclI TGATCA 1 cut(s) 15
BcoDI GTCTC 2 cut(s) 198, 602
BfaI CTAG 1 cut(s) 822
BfmI CTRYAG 2 cut(s) 99, 399
BisI GCNGC 1 cut(s) 716
BlsI GCNGC 1 cut(s) 717
BmcAI AGTACT 1 cut(s) 97
Bme1390I CCNGG 2 cut(s) 616, 663
Bme18I GGWCC 1 cut(s) 677
BmeRI GACNNNNNGTC 1 cut(s) 649
BmeT110I CYCGRG 1 cut(s) 1025
BmgT120I GGNCC 1 cut(s) 677
BmiI GGNNCC 7 cut(s) 130, 196, 253, 502, 549, 739, 1040
BmrFI CCNGG 2 cut(s) 616, 663
BmsI GCATC 3 cut(s) 155, 314, 459
BpiI GAAGAC 1 cut(s) 116
BpmI CTGGAG 1 cut(s) 519
Bpu14I TTCGAA 1 cut(s) 807
BpuEI CTTGAG 1 cut(s) 1028
BsaHI GRCGYC 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 198
BsaJI CCNNGG 3 cut(s) 328, 662, 951
BsaXI ACNNNNNCTCC 4 cut(s) 820, 850, 1022, 1052
Bsc4I CCNNNNNNNGG 2 cut(s) 261, 991
Bse1I ACTGG 1 cut(s) 502
Bse21I CCTNAGG 2 cut(s) 133, 378
Bse3DI GCAATG 1 cut(s) 581
BseBI CCWGG 2 cut(s) 616, 663
BseDI CCNNGG 3 cut(s) 328, 662, 951
BseGI GGATG 3 cut(s) 18, 329, 535
BseLI CCNNNNNNNGG 2 cut(s) 261, 991
BseMI GCAATG 1 cut(s) 581
BseMII CTCAG 3 cut(s) 36, 147, 392
BseNI ACTGG 1 cut(s) 502
BseRI GAGGAG 2 cut(s) 620, 1043
BseXI GCAGC 1 cut(s) 702
BshFI GGCC 1 cut(s) 846
BshNI GGYRCC 2 cut(s) 194, 737
BsiHKCI CYCGRG 1 cut(s) 1025
BslFI GGGAC 2 cut(s) 334, 1022
BslI CCNNNNNNNGG 2 cut(s) 261, 991
BsmAI GTCTC 2 cut(s) 198, 602
BsmFI GGGAC 2 cut(s) 334, 1022
BsnI GGCC 1 cut(s) 846
Bso31I GGTCTC 1 cut(s) 198
BsoBI CYCGRG 1 cut(s) 1025
Bsp119I TTCGAA 1 cut(s) 807
Bsp1286I GDGCHC 1 cut(s) 505
Bsp143I GATC 2 cut(s) 15, 547
BspANI GGCC 1 cut(s) 846
BspCNI CTCAG 3 cut(s) 37, 146, 391
BspHI TCATGA 1 cut(s) 729
BspLI GGNNCC 7 cut(s) 130, 196, 253, 502, 549, 739, 1040
BspMAI CTGCAG 1 cut(s) 403
BspPI GGATC 2 cut(s) 542, 555
BspT104I TTCGAA 1 cut(s) 807
BspT107I GGYRCC 2 cut(s) 194, 737
BspTNI GGTCTC 1 cut(s) 198
BsrDI GCAATG 1 cut(s) 581
BsrI ACTGG 1 cut(s) 502
BssECI CCNNGG 3 cut(s) 328, 662, 951
BssMI GATC 2 cut(s) 15, 547
BssNI GRCGYC 1 cut(s) 309
BssSI CACGAG 1 cut(s) 454
BssT1I CCWWGG 2 cut(s) 328, 951
Bst2BI CACGAG 1 cut(s) 454
Bst2UI CCWGG 2 cut(s) 616, 663
Bst4CI ACNGT 5 cut(s) 100, 115, 187, 199, 360
BstACI GRCGYC 1 cut(s) 309
BstBI TTCGAA 1 cut(s) 807
BstDEI CTNAG 5 cut(s) 45, 133, 378, 936, 1004
BstF5I GGATG 3 cut(s) 18, 329, 535
BstHHI GCGC 1 cut(s) 240
BstKTI GATC 2 cut(s) 18, 550
BstMAI GTCTC 2 cut(s) 198, 602
BstMBI GATC 2 cut(s) 15, 547
BstMWI GCNNNNNNNGC 1 cut(s) 169
BstNI CCWGG 2 cut(s) 616, 663
BstSCI CCNGG 2 cut(s) 614, 661
BstSFI CTRYAG 2 cut(s) 99, 399
BstV1I GCAGC 1 cut(s) 702
BstV2I GAAGAC 1 cut(s) 116
BstX2I RGATCY 1 cut(s) 547
BstYI RGATCY 1 cut(s) 547
Bsu36I CCTNAGG 2 cut(s) 133, 378
BsuRI GGCC 1 cut(s) 846
BtsCI GGATG 3 cut(s) 18, 329, 535
BtsI GCAGTG 1 cut(s) 408
BtsIMutI CAGTG 1 cut(s) 408
CciI TCATGA 1 cut(s) 729
CfoI GCGC 1 cut(s) 240
Cfr13I GGNCC 1 cut(s) 677
CseI GACGC 2 cut(s) 317, 336
Csp6I GTAC 4 cut(s) 96, 356, 541, 738
CviAII CATG 4 cut(s) 150, 432, 537, 730
CviQI GTAC 4 cut(s) 96, 356, 541, 738
DdeI CTNAG 5 cut(s) 45, 133, 378, 936, 1004
DpnI GATC 2 cut(s) 17, 549
DpnII GATC 2 cut(s) 15, 547
DriI GACNNNNNGTC 1 cut(s) 649
EaeI YGGCCR 1 cut(s) 844
Eam1105I GACNNNNNGTC 1 cut(s) 649
Eco130I CCWWGG 2 cut(s) 328, 951
Eco24I GRGCYC 1 cut(s) 505
Eco31I GGTCTC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 677
Eco57I CTGAAG 5 cut(s) 468, 704, 801, 912, 933
Eco81I CCTNAGG 2 cut(s) 133, 378
Eco88I CYCGRG 1 cut(s) 1025
EcoRII CCWGG 2 cut(s) 614, 661
EcoT14I CCWWGG 2 cut(s) 328, 951
EcoT38I GRGCYC 1 cut(s) 505
ErhI CCWWGG 2 cut(s) 328, 951
FaeI CATG 4 cut(s) 153, 435, 540, 733
FaiI YATR 9 cut(s) 143, 151, 183, 314, 342, 433, 538, 731, 795
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FaqI GGGAC 2 cut(s) 334, 1022
FatI CATG 4 cut(s) 149, 431, 536, 729
FbaI TGATCA 1 cut(s) 15
Fnu4HI GCNGC 1 cut(s) 716
FokI GGATG 3 cut(s) 5, 336, 542
FriOI GRGCYC 1 cut(s) 505
Fsp4HI GCNGC 1 cut(s) 716
FspBI CTAG 1 cut(s) 822
GlaI GCGC 1 cut(s) 239
GluI GCNGC 1 cut(s) 716
GsuI CTGGAG 1 cut(s) 519
HaeIII GGCC 1 cut(s) 846
HgaI GACGC 2 cut(s) 317, 336
HhaI GCGC 1 cut(s) 240
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 4 cut(s) 153, 435, 540, 733
Hin6I GCGC 1 cut(s) 238
HinP1I GCGC 1 cut(s) 238
HincII GTYRAC 2 cut(s) 669, 769
HindII GTYRAC 2 cut(s) 669, 769
HindIII AAGCTT 1 cut(s) 801
HinfI GANTC 4 cut(s) 215, 658, 932, 975
HpaI GTTAAC 1 cut(s) 669
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 3 cut(s) 669, 680, 769
Hpy188I TCNGA 6 cut(s) 292, 510, 517, 547, 723, 931
Hpy188III TCNNGA 5 cut(s) 203, 454, 730, 992, 1045
Hpy8I GTNNAC 3 cut(s) 669, 680, 769
Hpy99I CGWCG 2 cut(s) 311, 713
HpyAV CCTTC 7 cut(s) 98, 290, 400, 825, 936, 995, 1027
HpyCH4III ACNGT 5 cut(s) 100, 115, 187, 199, 360
HpyCH4IV ACGT 1 cut(s) 771
HpyCH4V TGCA 2 cut(s) 172, 401
HpyF10VI GCNNNNNNNGC 1 cut(s) 169
HpyF3I CTNAG 5 cut(s) 45, 133, 378, 936, 1004
HpySE526I ACGT 1 cut(s) 771
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 4 cut(s) 153, 435, 540, 733
HspAI GCGC 1 cut(s) 238
KpnI GGTACC 1 cut(s) 741
Ksp22I TGATCA 1 cut(s) 15
KspAI GTTAAC 1 cut(s) 669
Kzo9I GATC 2 cut(s) 15, 547
LmnI GCTCC 3 cut(s) 128, 251, 500
Lsp1109I GCAGC 1 cut(s) 702
LweI GCATC 3 cut(s) 155, 314, 459
MaeI CTAG 1 cut(s) 822
MaeII ACGT 1 cut(s) 771
MaeIII GTNAC 3 cut(s) 121, 137, 367
MalI GATC 2 cut(s) 17, 549
MboI GATC 2 cut(s) 15, 547
MboII GAAGA 3 cut(s) 121, 305, 465
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 1 cut(s) 505
MlsI TGGCCA 1 cut(s) 846
MluCI AATT 4 cut(s) 440, 620, 700, 810
MluNI TGGCCA 1 cut(s) 846
MlyI GAGTC 1 cut(s) 926
Mox20I TGGCCA 1 cut(s) 846
MroXI GAANNNNTTC 2 cut(s) 264, 1037
MscI TGGCCA 1 cut(s) 846
MseI TTAA 5 cut(s) 26, 417, 467, 668, 699
MslI CAYNNNNRTG 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 846
MspR9I CCNGG 2 cut(s) 616, 663
MvaI CCWGG 2 cut(s) 616, 663
MwoI GCNNNNNNNGC 1 cut(s) 169
NdeII GATC 2 cut(s) 15, 547
NlaIII CATG 4 cut(s) 153, 435, 540, 733
NlaIV GGNNCC 7 cut(s) 130, 196, 253, 502, 549, 739, 1040
NspV TTCGAA 1 cut(s) 807
OliI CACNNNNGTG 1 cut(s) 458
PaeR7I CTCGAG 1 cut(s) 1025
PagI TCATGA 1 cut(s) 729
PdmI GAANNNNTTC 2 cut(s) 264, 1037
PfeI GAWTC 3 cut(s) 215, 658, 975
PkrI GCNGC 1 cut(s) 717
PleI GAGTC 1 cut(s) 926
PpsI GAGTC 1 cut(s) 926
Psp6I CCWGG 2 cut(s) 614, 661
PspGI CCWGG 2 cut(s) 614, 661
PspN4I GGNNCC 7 cut(s) 130, 196, 253, 502, 549, 739, 1040
PspPI GGNCC 1 cut(s) 677
PspXI VCTCGAGB 1 cut(s) 1025
PstI CTGCAG 1 cut(s) 403
PsuI RGATCY 1 cut(s) 547
RsaI GTAC 4 cut(s) 97, 357, 542, 739
RsaNI GTAC 4 cut(s) 96, 356, 541, 738
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 5 cut(s) 26, 417, 467, 668, 699
SatI GCNGC 1 cut(s) 716
Sau3AI GATC 2 cut(s) 15, 547
Sau96I GGNCC 1 cut(s) 677
ScaI AGTACT 1 cut(s) 97
SchI GAGTC 1 cut(s) 926
ScrFI CCNGG 2 cut(s) 616, 663
SduI GDGCHC 1 cut(s) 505
SfaNI GCATC 3 cut(s) 155, 314, 459
SfcI CTRYAG 2 cut(s) 99, 399
Sfr274I CTCGAG 1 cut(s) 1025
SfuI TTCGAA 1 cut(s) 807
SinI GGWCC 1 cut(s) 677
SlaI CTCGAG 1 cut(s) 1025
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 2 cut(s) 1025, 1043
SmoI CTYRAG 2 cut(s) 1025, 1043
Sse9I AATT 4 cut(s) 440, 620, 700, 810
SspMI CTAG 1 cut(s) 822
StyD4I CCNGG 2 cut(s) 614, 661
StyI CCWWGG 2 cut(s) 328, 951
TaaI ACNGT 5 cut(s) 100, 115, 187, 199, 360
TaiI ACGT 1 cut(s) 774
TaqI TCGA 5 cut(s) 202, 306, 711, 807, 1026
TasI AATT 4 cut(s) 440, 620, 700, 810
TatI WGTACW 2 cut(s) 95, 355
TfiI GAWTC 3 cut(s) 215, 658, 975
Tru1I TTAA 5 cut(s) 26, 417, 467, 668, 699
Tru9I TTAA 5 cut(s) 26, 417, 467, 668, 699
TscAI CASTG 1 cut(s) 408
TseI GCWGC 1 cut(s) 715
TspDTI ATGAA 4 cut(s) 257, 329, 546, 718
TspGWI ACGGA 2 cut(s) 264, 697
TspRI CASTG 1 cut(s) 408
VpaK11BI GGWCC 1 cut(s) 677
XapI RAATTY 3 cut(s) 440, 620, 810
XhoI CTCGAG 1 cut(s) 1025
XmnI GAANNNNTTC 2 cut(s) 264, 1037
XspI CTAG 1 cut(s) 822
ZrmI AGTACT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.