Rh1DG045100

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000709
Physical Location & Seq
Forward (+)
7429409 .. 7432593
3185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG045100.1

Sequence Viewer

Length: 972 bp
ATGAGCGGAGCAGGGAAAGGTGTTGTCTGTGTAACAGGAGCATCTGGTTATATTGCATCATGGCTGGTCAAGCTTTTGCTACAACGTGGTTATATTGTCAAAGGCACTGTTCGGGACCCAAACGATCCCAAAAAAACAGAACACTTACTTTCACTTGAGGGGGCAAAAGAAAGGCTTCATTTGTTCAAAGCAGATTTGCTAGATGAAGGATCTTTTGACCCTGCAGTTGATGGATGTGACTGTGTTTTTCATACCGCATCTCCGGTATCTTTTTCATCAGTCAATGACCCTCAGGCAGAACTAATTGACCCTGCTGTGAAGGGAACATTAAATGTTCTGAAATCATGTGCAAAATTTCCAACCGTCAAGCGAGTGGTTTTGACATCTTCTTTGGCTTCAGTAATGATGAATGGAAAACCTCTGACCCCTGATGTTGTAGTGGATGAAACATGGTATTCAGATCCACTAATTGCTGAGAAGCACAAGCTGTGGTATATTCTTTCAAAGACTTTAGCTGAGGAGGCTGCTTGGAAATTTGCTGAAGGAAATAGGATTGACTTGGTTTCAATAAATCCGGGGTTTGTGATTGGACCACTCTTACAGCCGACAATTAATTTGACGGTGGAGATGATTCAGAACCTCATAACTGGCAAGGCAGTGCGCGCTTCAAATTACAGATTTATTGATGTTAGAGATGTTGCCGCTGCACATATTCAAGCATTTGAAATTCTTTCAGCTAGTGGAAGGTATTGTTTGGTCAGCCATGTTGCAAATATTTCCGAGACTCTAGAGATGATACGACAACTTTATCCCACAATGTACATTCCTGAAAGATGTGAGGATCCTTCTGGCCCACAGTTTCAAGTATCCAAGGAGAAAGCAAAAAGTCTGGGAATTAGTTTTCATCCATTGGAAGTAACTCTGAAGGACACTATTGAAAGCCTGAAGGAGAAGGGTTTCCTCAACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

323

Amino Acids

35.43

Weight (kDa)

6.19

Isoelectric Point (pI)

25.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 106 1.2e-08 NmrA-like family
Epimerase PF01370 9 - 242 1.8e-24 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 246 4.2e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 130 2.5e-12 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 201 2.9e-11 Male sterility protein
NAD_binding_10 PF13460 13 - 133 7.5e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 6
AccII CGCG 1 cut(s) 663
AciI CCGC 3 cut(s) 6, 255, 702
AclWI GGATC 5 cut(s) 119, 217, 455, 836, 849
AcsI RAATTY 3 cut(s) 353, 533, 726
AcuI CTGAAG 4 cut(s) 381, 561, 944, 965
AfaI GTAC 1 cut(s) 821
AgsI TTSAA 8 cut(s) 187, 504, 567, 669, 716, 725, 863, 938
AluBI AGCT 4 cut(s) 73, 487, 515, 737
AluI AGCT 4 cut(s) 73, 487, 515, 737
Alw26I GTCTC 1 cut(s) 776
AlwI GGATC 5 cut(s) 119, 217, 455, 836, 849
AoxI GGCC 1 cut(s) 850
ApeKI GCWGC 2 cut(s) 524, 704
ApoI RAATTY 3 cut(s) 353, 533, 726
ArsI GACNNNNNNTTYG 4 cut(s) 373, 405, 598, 630
AseI ATTAAT 1 cut(s) 612
Asp700I GAANNNNTTC 2 cut(s) 174, 956
AspLEI GCGC 2 cut(s) 663, 665
AspS9I GGNCC 3 cut(s) 115, 590, 851
AsuC2I CCSGG 1 cut(s) 576
AvaII GGWCC 2 cut(s) 115, 590
AxyI CCTNAGG 1 cut(s) 291
BamHI GGATCC 1 cut(s) 841
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 2 cut(s) 511, 691
BccI CCATC 1 cut(s) 224
BciVI GTATCC 1 cut(s) 877
BcnI CCSGG 1 cut(s) 576
BcoDI GTCTC 1 cut(s) 776
BfaI CTAG 3 cut(s) 200, 738, 788
BfmI CTRYAG 1 cut(s) 222
BfuI GTATCC 1 cut(s) 877
BisI GCNGC 3 cut(s) 525, 702, 705
BlsI GCNGC 3 cut(s) 526, 703, 706
Bme1390I CCNGG 1 cut(s) 576
Bme18I GGWCC 2 cut(s) 115, 590
BmgT120I GGNCC 3 cut(s) 115, 590, 851
BmiI GGNNCC 3 cut(s) 116, 117, 843
BmrFI CCNGG 1 cut(s) 576
BmsI GCATC 3 cut(s) 50, 65, 266
Bpu10I CCTNAGC 1 cut(s) 516
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 576
BsaJI CCNNGG 2 cut(s) 575, 870
BsaWI WCCGGW 1 cut(s) 262
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bse1I ACTGG 1 cut(s) 652
Bse21I CCTNAGG 1 cut(s) 291
BseDI CCNNGG 2 cut(s) 575, 870
BseGI GGATG 3 cut(s) 239, 448, 904
BseMII CTCAG 3 cut(s) 305, 465, 507
BseNI ACTGG 1 cut(s) 652
BsePI GCGCGC 1 cut(s) 661
BseRI GAGGAG 1 cut(s) 533
BseXI GCAGC 2 cut(s) 511, 691
BsgI GTGCAG 1 cut(s) 690
Bsh1236I CGCG 1 cut(s) 663
BshFI GGCC 1 cut(s) 852
BsiSI CCGG 2 cut(s) 263, 575
BslFI GGGAC 1 cut(s) 128
BsmAI GTCTC 1 cut(s) 776
BsmFI GGGAC 1 cut(s) 128
BsnI GGCC 1 cut(s) 852
Bsp1407I TGTACA 1 cut(s) 819
Bsp143I GATC 4 cut(s) 124, 209, 460, 841
BspACI CCGC 3 cut(s) 6, 255, 702
BspANI GGCC 1 cut(s) 852
BspCNI CTCAG 3 cut(s) 304, 466, 508
BspFNI CGCG 1 cut(s) 663
BspLI GGNNCC 3 cut(s) 116, 117, 843
BspMAI CTGCAG 1 cut(s) 226
BspPI GGATC 5 cut(s) 119, 217, 455, 836, 849
BsrBI CCGCTC 1 cut(s) 6
BsrGI TGTACA 1 cut(s) 819
BsrI ACTGG 1 cut(s) 652
BssECI CCNNGG 2 cut(s) 575, 870
BssHII GCGCGC 1 cut(s) 661
BssMI GATC 4 cut(s) 124, 209, 460, 841
BssT1I CCWWGG 1 cut(s) 870
Bst4CI ACNGT 5 cut(s) 109, 242, 364, 622, 858
BstAUI TGTACA 1 cut(s) 819
BstC8I GCNNGC 1 cut(s) 663
BstDEI CTNAG 3 cut(s) 291, 474, 516
BstF5I GGATG 3 cut(s) 239, 448, 904
BstFNI CGCG 1 cut(s) 663
BstHHI GCGC 2 cut(s) 663, 665
BstKTI GATC 4 cut(s) 127, 212, 463, 844
BstMAI GTCTC 1 cut(s) 776
BstMBI GATC 4 cut(s) 124, 209, 460, 841
BstMWI GCNNNNNNNGC 3 cut(s) 70, 521, 662
BstSCI CCNGG 1 cut(s) 574
BstSFI CTRYAG 1 cut(s) 222
BstUI CGCG 1 cut(s) 663
BstV1I GCAGC 2 cut(s) 511, 691
BstX2I RGATCY 3 cut(s) 209, 460, 841
BstYI RGATCY 3 cut(s) 209, 460, 841
Bsu36I CCTNAGG 1 cut(s) 291
BsuI GTATCC 1 cut(s) 877
BsuRI GGCC 1 cut(s) 852
BtsCI GGATG 3 cut(s) 239, 448, 904
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 2 cut(s) 105, 663
Cac8I GCNNGC 1 cut(s) 663
CfoI GCGC 2 cut(s) 663, 665
Cfr13I GGNCC 3 cut(s) 115, 590, 851
Csp6I GTAC 1 cut(s) 820
CviAII CATG 4 cut(s) 60, 345, 450, 764
CviQI GTAC 1 cut(s) 820
DdeI CTNAG 3 cut(s) 291, 474, 516
DpnI GATC 4 cut(s) 126, 211, 462, 843
DpnII GATC 4 cut(s) 124, 209, 460, 841
Eco130I CCWWGG 1 cut(s) 870
Eco47I GGWCC 2 cut(s) 115, 590
Eco57I CTGAAG 4 cut(s) 381, 561, 944, 965
Eco81I CCTNAGG 1 cut(s) 291
EcoO109I RGGNCCY 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 870
ErhI CCWWGG 1 cut(s) 870
FaeI CATG 4 cut(s) 63, 348, 453, 767
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 1 cut(s) 128
FatI CATG 4 cut(s) 59, 344, 449, 763
FauNDI CATATG 1 cut(s) 968
Fnu4HI GCNGC 3 cut(s) 525, 702, 705
FokI GGATG 3 cut(s) 246, 455, 891
Fsp4HI GCNGC 3 cut(s) 525, 702, 705
FspBI CTAG 3 cut(s) 200, 738, 788
GlaI GCGC 2 cut(s) 662, 664
GluI GCNGC 3 cut(s) 525, 702, 705
HaeIII GGCC 1 cut(s) 852
HapII CCGG 2 cut(s) 263, 575
HhaI GCGC 2 cut(s) 663, 665
Hin1II CATG 4 cut(s) 63, 348, 453, 767
Hin6I GCGC 2 cut(s) 661, 663
HinP1I GCGC 2 cut(s) 661, 663
HindIII AAGCTT 1 cut(s) 71
HinfI GANTC 2 cut(s) 631, 784
HpaII CCGG 2 cut(s) 263, 575
Hpy188I TCNGA 6 cut(s) 339, 423, 460, 636, 781, 924
Hpy188III TCNNGA 3 cut(s) 113, 788, 827
HpyAV CCTTC 8 cut(s) 200, 313, 536, 738, 855, 919, 940, 946
HpyCH4III ACNGT 5 cut(s) 109, 242, 364, 622, 858
HpyCH4IV ACGT 1 cut(s) 85
HpyCH4V TGCA 5 cut(s) 56, 224, 350, 707, 770
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 521, 662
HpyF3I CTNAG 3 cut(s) 291, 474, 516
HpySE526I ACGT 1 cut(s) 85
Hsp92II CATG 4 cut(s) 63, 348, 453, 767
HspAI GCGC 2 cut(s) 661, 663
KflI GGGWCCC 1 cut(s) 115
Kzo9I GATC 4 cut(s) 124, 209, 460, 841
LmnI GCTCC 2 cut(s) 8, 38
Lsp1109I GCAGC 2 cut(s) 511, 691
LweI GCATC 3 cut(s) 50, 65, 266
MaeI CTAG 3 cut(s) 200, 738, 788
MaeII ACGT 1 cut(s) 85
MaeIII GTNAC 3 cut(s) 31, 236, 916
MalI GATC 4 cut(s) 126, 211, 462, 843
MbiI CCGCTC 1 cut(s) 6
MboI GATC 4 cut(s) 124, 209, 460, 841
MboII GAAGA 1 cut(s) 378
MflI RGATCY 3 cut(s) 209, 460, 841
MluCI AATT 9 cut(s) 303, 353, 468, 533, 609, 613, 670, 726, 894
MlyI GAGTC 1 cut(s) 778
MmeI TCCRAC 1 cut(s) 383
MnlI CCTC 8 cut(s) 151, 300, 429, 511, 514, 650, 832, 971
MroXI GAANNNNTTC 2 cut(s) 174, 956
MseI TTAA 2 cut(s) 329, 612
MspA1I CMGCKG 1 cut(s) 704
MspI CCGG 2 cut(s) 263, 575
MspR9I CCNGG 1 cut(s) 576
MvnI CGCG 1 cut(s) 663
MwoI GCNNNNNNNGC 3 cut(s) 70, 521, 662
NciI CCSGG 1 cut(s) 576
NdeI CATATG 1 cut(s) 968
NdeII GATC 4 cut(s) 124, 209, 460, 841
NlaIII CATG 4 cut(s) 63, 348, 453, 767
NlaIV GGNNCC 3 cut(s) 116, 117, 843
NmuCI GTSAC 1 cut(s) 236
PauI GCGCGC 1 cut(s) 661
PdmI GAANNNNTTC 2 cut(s) 174, 956
PfeI GAWTC 1 cut(s) 631
PkrI GCNGC 3 cut(s) 526, 703, 706
PleI GAGTC 1 cut(s) 778
PpsI GAGTC 1 cut(s) 778
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 612
Psp5II RGGWCCY 1 cut(s) 115
PspN4I GGNNCC 3 cut(s) 116, 117, 843
PspPI GGNCC 3 cut(s) 115, 590, 851
PspPPI RGGWCCY 1 cut(s) 115
PstI CTGCAG 1 cut(s) 226
PsuI RGATCY 3 cut(s) 209, 460, 841
PteI GCGCGC 1 cut(s) 661
RsaI GTAC 1 cut(s) 821
RsaNI GTAC 1 cut(s) 820
SaqAI TTAA 2 cut(s) 329, 612
SatI GCNGC 3 cut(s) 525, 702, 705
Sau3AI GATC 4 cut(s) 124, 209, 460, 841
Sau96I GGNCC 3 cut(s) 115, 590, 851
SchI GAGTC 1 cut(s) 778
ScrFI CCNGG 1 cut(s) 576
SetI ASST 9 cut(s) 22, 75, 88, 421, 489, 517, 642, 739, 749
SfaNI GCATC 3 cut(s) 50, 65, 266
SfcI CTRYAG 1 cut(s) 222
SinI GGWCC 2 cut(s) 115, 590
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 9 cut(s) 303, 353, 468, 533, 609, 613, 670, 726, 894
SsiI CCGC 3 cut(s) 6, 255, 702
SspI AATATT 1 cut(s) 775
SspMI CTAG 3 cut(s) 200, 738, 788
StyD4I CCNGG 1 cut(s) 574
StyI CCWWGG 1 cut(s) 870
TaaI ACNGT 5 cut(s) 109, 242, 364, 622, 858
TaiI ACGT 1 cut(s) 88
TasI AATT 9 cut(s) 303, 353, 468, 533, 609, 613, 670, 726, 894
TatI WGTACW 1 cut(s) 819
TauI GCSGC 1 cut(s) 704
TfiI GAWTC 1 cut(s) 631
Tru1I TTAA 2 cut(s) 329, 612
Tru9I TTAA 2 cut(s) 329, 612
TscAI CASTG 2 cut(s) 112, 663
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 2 cut(s) 524, 704
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 7 cut(s) 167, 219, 239, 264, 422, 459, 893
TspRI CASTG 2 cut(s) 112, 663
VpaK11BI GGWCC 2 cut(s) 115, 590
VspI ATTAAT 1 cut(s) 612
XapI RAATTY 3 cut(s) 353, 533, 726
XbaI TCTAGA 1 cut(s) 787
XmnI GAANNNNTTC 2 cut(s) 174, 956
XspI CTAG 3 cut(s) 200, 738, 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.