Rroxscaffold_4G00330450

NmrA-like family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000709
Physical Location & Seq
Forward (+)
64172966 .. 64174909
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00330450.1

Sequence Viewer

Length: 978 bp
ATGAGCGGAGAAAGGAAGGTTGTGTGTGTTACAGGAGCTTCTGGTTTCATAGCTTCATGGCTGGTGAAACTCTTGTTACAGCGAGGTTATGTAGTCAAAGCCACCGTTCGTGACCCAAATAATCCGAAGAAAACAGAACACTTGCTCTCACTCGAGGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAGGGGTCTTTTGACCTTGTAGTTGATGGATGTGAAGGTGTTTTCCACACAGCATCTCCGGTACTATTTTCATCGATCAGCGACCCACAGGCAGAACTGATTGATCCTGCAGTAAAGGGAACGCTTAATGTTCTTAAATCGTGCGTGAAAATTCCAACAATCAAGAGGGTAGTTTTAACATCTTCCATGGCGGCAGTTGTGTTCAATGGAAGATCTTTAACCTCTAATGTGGTAATTGATGAAACATGGTTTTCTGATCCACTTGTTTGTGAGGAGTTGAAGGAATGGTATTTTCTTTCGAAAACTTTAGCAGAGAAGGCGGCTTGGAAATTTGCTGAGGAAAACGGGATTGACATGGTGACCATAAATCCCTCGTATGTGATCGGTCCGCTCCTGCAGCCAACTCTTAATTTCAGTGTGGAGATGGTTCTGGATCTCAAGAAGGATATCGACGGAGTAACTACTGCAAATTATACATCTACTGATGTTAGAGATGTTGCTTCTGCACATATTCAAGCATTTGAAGTTCCTTCAGCTAGTGGAAGATATTGTTTAGTTGCAAATGTCACCCCCATTCACGAGGCTCTGAAGATTTTAAAGGAACTTCATCCTACTTTGTGCCCAACTGAAATATGTGAGCAGGATATCCCTTCTGCTCCAGAGTATCAAATATCCCAGGAAAAGGCAAAAAGTTTGGGACTTGATTTCCTTCCGTTGGAAGTAAGTCTCAGGGACACTATTGAATGCCTGAAGGAGAAGGGTTTCCTTGAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

325

Amino Acids

35.73

Weight (kDa)

5.07

Isoelectric Point (pI)

28.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 105 6.8e-08 NmrA-like family
Epimerase PF01370 8 - 246 1.3e-21 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 241 1.3e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 196 4.2e-14 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 198 1.7e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 133 9.7e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 6, 592
AciI CCGC 4 cut(s) 6, 392, 521, 590
AclWI GGATC 3 cut(s) 299, 452, 642
AcsI RAATTY 2 cut(s) 351, 530
AcuI CTGAAG 3 cut(s) 717, 809, 971
AfaI GTAC 1 cut(s) 264
AfiI CCNNNNNNNGG 2 cut(s) 883, 916
AgsI TTSAA 6 cut(s) 184, 406, 481, 716, 725, 944
AjnI CCWGG 1 cut(s) 876
AluBI AGCT 3 cut(s) 38, 53, 737
AluI AGCT 3 cut(s) 38, 53, 737
Alw26I GTCTC 1 cut(s) 932
AlwI GGATC 3 cut(s) 299, 452, 642
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 1 cut(s) 972
ApeKI GCWGC 1 cut(s) 598
ApoI RAATTY 2 cut(s) 351, 530
Asp700I GAANNNNTTC 2 cut(s) 171, 962
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 3 cut(s) 76, 571, 760
AsuII TTCGAA 1 cut(s) 500
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 1 cut(s) 587
BaeGI GKGCMC 1 cut(s) 824
BauI CACGAG 1 cut(s) 779
BbvCI CCTCAGC 1 cut(s) 537
BbvI GCAGC 1 cut(s) 610
BccI CCATC 2 cut(s) 221, 619
BciT130I CCWGG 1 cut(s) 878
BcoDI GTCTC 1 cut(s) 932
BfaI CTAG 1 cut(s) 738
BfmI CTRYAG 2 cut(s) 309, 596
BglII AGATCT 1 cut(s) 413
BisI GCNGC 3 cut(s) 393, 522, 599
BlsI GCNGC 3 cut(s) 394, 523, 600
Bme1390I CCNGG 1 cut(s) 878
Bme18I GGWCC 1 cut(s) 587
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 1 cut(s) 587
BmrFI CCNGG 1 cut(s) 878
BmsI GCATC 1 cut(s) 263
BpmI CTGGAG 1 cut(s) 843
Bpu10I CCTNAGC 1 cut(s) 537
Bpu14I TTCGAA 1 cut(s) 500
BpuEI CTTGAG 1 cut(s) 623
Bsa29I ATCGAT 1 cut(s) 275
BsaJI CCNNGG 2 cut(s) 387, 876
BsaWI WCCGGW 1 cut(s) 259
BsaXI ACNNNNNCTCC 2 cut(s) 241, 271
Bsc4I CCNNNNNNNGG 2 cut(s) 883, 916
BseBI CCWGG 1 cut(s) 878
BseCI ATCGAT 1 cut(s) 275
BseDI CCNNGG 2 cut(s) 387, 876
BseGI GGATG 2 cut(s) 236, 808
BseLI CCNNNNNNNGG 2 cut(s) 883, 916
BseMII CTCAG 2 cut(s) 528, 943
BseRI GAGGAG 1 cut(s) 488
BseSI GKGCMC 1 cut(s) 824
BseXI GCAGC 1 cut(s) 610
BsgI GTGCAG 1 cut(s) 690
BshFI GGCC 1 cut(s) 974
BshVI ATCGAT 1 cut(s) 275
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 1 cut(s) 260
BslFI GGGAC 2 cut(s) 912, 947
BslI CCNNNNNNNGG 2 cut(s) 883, 916
BsmAI GTCTC 1 cut(s) 932
BsmFI GGGAC 2 cut(s) 912, 947
BsmI GAATGC 1 cut(s) 950
BsnI GGCC 1 cut(s) 974
BsoBI CYCGRG 1 cut(s) 152
Bsp119I TTCGAA 1 cut(s) 500
Bsp1286I GDGCHC 1 cut(s) 824
Bsp143I GATC 6 cut(s) 276, 304, 413, 457, 582, 634
Bsp19I CCATGG 1 cut(s) 387
BspACI CCGC 4 cut(s) 6, 392, 521, 590
BspANI GGCC 1 cut(s) 974
BspCNI CTCAG 2 cut(s) 529, 942
BspDI ATCGAT 1 cut(s) 275
BspMAI CTGCAG 2 cut(s) 313, 600
BspPI GGATC 3 cut(s) 299, 452, 642
BspT104I TTCGAA 1 cut(s) 500
BsrBI CCGCTC 2 cut(s) 6, 592
BssECI CCNNGG 2 cut(s) 387, 876
BssMI GATC 6 cut(s) 276, 304, 413, 457, 582, 634
BssSI CACGAG 1 cut(s) 779
BssT1I CCWWGG 1 cut(s) 387
Bst2BI CACGAG 1 cut(s) 779
Bst2UI CCWGG 1 cut(s) 878
Bst4CI ACNGT 1 cut(s) 106
Bst6I CTCTTC 1 cut(s) 195
BstBI TTCGAA 1 cut(s) 500
BstDEI CTNAG 2 cut(s) 537, 929
BstDSI CCRYGG 1 cut(s) 387
BstEII GGTNACC 1 cut(s) 559
BstF5I GGATG 2 cut(s) 236, 808
BstKTI GATC 6 cut(s) 279, 307, 416, 460, 585, 637
BstMAI GTCTC 1 cut(s) 932
BstMBI GATC 6 cut(s) 276, 304, 413, 457, 582, 634
BstMWI GCNNNNNNNGC 2 cut(s) 518, 598
BstNI CCWGG 1 cut(s) 878
BstPI GGTNACC 1 cut(s) 559
BstSCI CCNGG 1 cut(s) 876
BstSFI CTRYAG 2 cut(s) 309, 596
BstSLI GKGCMC 1 cut(s) 824
BstV1I GCAGC 1 cut(s) 610
BstX2I RGATCY 2 cut(s) 413, 634
BstYI RGATCY 2 cut(s) 413, 634
Bsu15I ATCGAT 1 cut(s) 275
BsuRI GGCC 1 cut(s) 974
BsuTUI ATCGAT 1 cut(s) 275
BtgI CCRYGG 1 cut(s) 387
BtsCI GGATG 2 cut(s) 236, 808
BtsIMutI CAGTG 1 cut(s) 622
Cfr13I GGNCC 1 cut(s) 587
ClaI ATCGAT 1 cut(s) 275
CpoI CGGWCCG 1 cut(s) 587
Csp6I GTAC 1 cut(s) 263
CspI CGGWCCG 1 cut(s) 587
CviAII CATG 4 cut(s) 57, 388, 447, 556
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 2 cut(s) 537, 929
DpnI GATC 6 cut(s) 278, 306, 415, 459, 584, 636
DpnII GATC 6 cut(s) 276, 304, 413, 457, 582, 634
DraI TTTAAA 1 cut(s) 798
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Eco130I CCWWGG 1 cut(s) 387
Eco147I AGGCCT 1 cut(s) 974
Eco32I GATATC 2 cut(s) 649, 847
Eco47I GGWCC 1 cut(s) 587
Eco57I CTGAAG 3 cut(s) 717, 809, 971
Eco88I CYCGRG 1 cut(s) 152
Eco91I GGTNACC 1 cut(s) 559
EcoO65I GGTNACC 1 cut(s) 559
EcoRII CCWGG 1 cut(s) 876
EcoRV GATATC 2 cut(s) 649, 847
EcoT14I CCWWGG 1 cut(s) 387
ErhI CCWWGG 1 cut(s) 387
FaeI CATG 4 cut(s) 60, 391, 450, 559
FaqI GGGAC 2 cut(s) 912, 947
FatI CATG 4 cut(s) 56, 387, 446, 555
Fnu4HI GCNGC 3 cut(s) 393, 522, 599
FokI GGATG 2 cut(s) 243, 795
Fsp4HI GCNGC 3 cut(s) 393, 522, 599
FspBI CTAG 1 cut(s) 738
GluI GCNGC 3 cut(s) 393, 522, 599
GsuI CTGGAG 1 cut(s) 843
HaeIII GGCC 1 cut(s) 974
HapII CCGG 1 cut(s) 260
Hin1II CATG 4 cut(s) 60, 391, 450, 559
HpaII CCGG 1 cut(s) 260
HphI GGTGA 3 cut(s) 76, 571, 760
Hpy188I TCNGA 3 cut(s) 126, 457, 789
Hpy188III TCNNGA 6 cut(s) 110, 364, 632, 640, 779, 860
Hpy99I CGWCG 1 cut(s) 656
HpyCH4III ACNGT 1 cut(s) 106
HpyCH4V TGCA 5 cut(s) 311, 598, 668, 707, 761
HpyF10VI GCNNNNNNNGC 2 cut(s) 518, 598
HpyF3I CTNAG 2 cut(s) 537, 929
Hsp92II CATG 4 cut(s) 60, 391, 450, 559
Kzo9I GATC 6 cut(s) 276, 304, 413, 457, 582, 634
LmnI GCTCC 4 cut(s) 35, 158, 597, 862
Lsp1109I GCAGC 1 cut(s) 610
LweI GCATC 1 cut(s) 263
MaeI CTAG 1 cut(s) 738
MaeIII GTNAC 6 cut(s) 28, 75, 110, 559, 658, 766
MalI GATC 6 cut(s) 278, 306, 415, 459, 584, 636
MbiI CCGCTC 2 cut(s) 6, 592
MboI GATC 6 cut(s) 276, 304, 413, 457, 582, 634
MboII GAAGA 6 cut(s) 139, 212, 375, 423, 756, 802
MflI RGATCY 2 cut(s) 413, 634
MhlI GDGCHC 1 cut(s) 824
MluCI AATT 5 cut(s) 351, 435, 530, 610, 670
MmeI TCCRAC 2 cut(s) 380, 897
MroXI GAANNNNTTC 2 cut(s) 171, 962
MseI TTAA 6 cut(s) 327, 336, 377, 419, 609, 797
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 878
Mva1269I GAATGC 1 cut(s) 950
MvaI CCWGG 1 cut(s) 878
MwoI GCNNNNNNNGC 2 cut(s) 518, 598
NcoI CCATGG 1 cut(s) 387
NdeII GATC 6 cut(s) 276, 304, 413, 457, 582, 634
NlaIII CATG 4 cut(s) 60, 391, 450, 559
NmuCI GTSAC 3 cut(s) 110, 559, 766
NspV TTCGAA 1 cut(s) 500
PaeR7I CTCGAG 1 cut(s) 152
PceI AGGCCT 1 cut(s) 974
PctI GAATGC 1 cut(s) 950
PdmI GAANNNNTTC 2 cut(s) 171, 962
PkrI GCNGC 3 cut(s) 394, 523, 600
Psp6I CCWGG 1 cut(s) 876
PspEI GGTNACC 1 cut(s) 559
PspGI CCWGG 1 cut(s) 876
PspPI GGNCC 1 cut(s) 587
PspXI VCTCGAGB 1 cut(s) 152
PstI CTGCAG 2 cut(s) 313, 600
PsuI RGATCY 2 cut(s) 413, 634
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
Rsr2I CGGWCCG 1 cut(s) 587
RsrII CGGWCCG 1 cut(s) 587
SaqAI TTAA 6 cut(s) 327, 336, 377, 419, 609, 797
SatI GCNGC 3 cut(s) 393, 522, 599
Sau3AI GATC 6 cut(s) 276, 304, 413, 457, 582, 634
Sau96I GGNCC 1 cut(s) 587
ScrFI CCNGG 1 cut(s) 878
SduI GDGCHC 1 cut(s) 824
SetI ASST 8 cut(s) 21, 40, 55, 88, 219, 241, 425, 739
SfaNI GCATC 1 cut(s) 263
SfcI CTRYAG 2 cut(s) 309, 596
Sfr274I CTCGAG 1 cut(s) 152
SfuI TTCGAA 1 cut(s) 500
SinI GGWCC 1 cut(s) 587
SlaI CTCGAG 1 cut(s) 152
SmlI CTYRAG 3 cut(s) 152, 638, 968
SmoI CTYRAG 3 cut(s) 152, 638, 968
Sse9I AATT 5 cut(s) 351, 435, 530, 610, 670
SseBI AGGCCT 1 cut(s) 974
SsiI CCGC 4 cut(s) 6, 392, 521, 590
SspMI CTAG 1 cut(s) 738
StuI AGGCCT 1 cut(s) 974
StyD4I CCNGG 1 cut(s) 876
StyI CCWWGG 1 cut(s) 387
TaaI ACNGT 1 cut(s) 106
TaqI TCGA 4 cut(s) 153, 275, 500, 651
TaqII GACCGA 1 cut(s) 575
TasI AATT 5 cut(s) 351, 435, 530, 610, 670
TauI GCSGC 2 cut(s) 395, 524
Tru1I TTAA 6 cut(s) 327, 336, 377, 419, 609, 797
Tru9I TTAA 6 cut(s) 327, 336, 377, 419, 609, 797
TscAI CASTG 1 cut(s) 622
TseFI GTSAC 3 cut(s) 110, 559, 766
TseI GCWGC 1 cut(s) 598
Tsp45I GTSAC 3 cut(s) 110, 559, 766
TspDTI ATGAA 6 cut(s) 37, 45, 164, 261, 456, 797
TspGWI ACGGA 2 cut(s) 669, 903
TspRI CASTG 1 cut(s) 622
VpaK11BI GGWCC 1 cut(s) 587
XapI RAATTY 2 cut(s) 351, 530
XhoI CTCGAG 1 cut(s) 152
XmnI GAANNNNTTC 2 cut(s) 171, 962
XspI CTAG 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.