MD02G1303400.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000709
Physical Location & Seq
Forward (+)
35717207 .. 35719521
2315 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1303400.v1.1.491

Sequence Viewer

Length: 963 bp
ATGAGTACTGTAGAAGGGAAGACTGTGTGCGTAACAGGAGCCTCAGGTTACATAGCATCATGGTTGGTGAAGCTCTTATTGCAAAAGGGTTATACTGTCAAAGGCACCGTTCGAGACCCAAATGATTCAAAGAAAACAGAACACTTGCGCTCATTAGACGGAGCCAAAGAAAGGCTTCATTTGTTCAAAGCAGATTTATTAGAAGAAGGGTCTTTCGACGCCGTAGTTGATGGATGCCAAGGTGTTTTTCATACAGCGTCCCCTGTACTGCTTTCAGTTACTGACCCTCAGGGAGAACTACTTGACCCTGCAGTGAAGGGAACACTTAATGTTCTACAATCATGCGCGAAATTTCGTAGCATCACGAGAGTGGTTTTAACATCTTCACTTGCTTCAGTAATGATGACTGGAGCCCCTCTGACCTCTGATGTGGTTTTGGATGAAACATGGTATTCGGATCCACTTTTTTGTGAGAAGGACAAGCAATGGTATTTGCTCTCAAAAACTTTAGCCGAGGAGACTGCCTGGAAATTTGCTAAAGGAAATGGGATTGACTTGGTGTCGTTGAATCCAGGGTTAACCATTGGTCCGCTCTTACAACCAACACTTAATTTCTCCGTCGAGCTGCTTCGGAACTTCATGAGTGGTACCCCAACAACTTTCAGTAATCCATTCGTTGACGTTAGAGATGTTGCCTCTGCTCATATTCAGGCTTTCGAAATTCCTTCAGCTAGTGGGAGATATTGTTTAGTTGGTCAAGTTGCTGACGACCTTGATACTCTAAAGATTTTACGGCAACTTTACCCGACTTTGACCCTTAATGAAGTTGTCAATCCCTCCGACTCAAAGTATAAAGTGTCCAAGGAGAAAGCAGAAGGTTTGGGAATCACTTTCCTTCCTCTGGAAACAAGTCTGAGGGACACTATTGAAAGCCTCAAGGAGAAGGGCTTCCTCAAGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

321

Amino Acids

34.95

Weight (kDa)

5.52

Isoelectric Point (pI)

27.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 87 1.1e-08 NmrA-like family
Epimerase PF01370 9 - 244 2.2e-23 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 238 5.5e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 129 3.7e-13 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 196 3.8e-09 Male sterility protein
NAD_binding_10 PF13460 13 - 131 4.4e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 647
AccB1I GGYRCC 2 cut(s) 104, 647
AccBSI CCGCTC 1 cut(s) 592
AccII CGCG 1 cut(s) 347
AciI CCGC 1 cut(s) 590
AclWI GGATC 2 cut(s) 452, 465
AcsI RAATTY 3 cut(s) 350, 530, 720
AcuI CTGAAG 2 cut(s) 378, 711
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 3 cut(s) 7, 267, 649
AfiI CCNNNNNNNGG 2 cut(s) 171, 901
AgsI TTSAA 4 cut(s) 129, 187, 568, 929
AhdI GACNNNNNGTC 1 cut(s) 559
AjnI CCWGG 2 cut(s) 524, 571
AjuI GAANNNNNNNTTGG 2 cut(s) 231, 263
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 3 cut(s) 73, 625, 731
AluI AGCT 3 cut(s) 73, 625, 731
Alw26I GTCTC 2 cut(s) 108, 512
AlwI GGATC 2 cut(s) 452, 465
AlwNI CAGNNNCTG 1 cut(s) 281
ApeKI GCWGC 1 cut(s) 625
ApoI RAATTY 3 cut(s) 350, 530, 720
Asp700I GAANNNNTTC 2 cut(s) 174, 947
Asp718I GGTACC 1 cut(s) 647
AspLEI GCGC 2 cut(s) 150, 347
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 1 cut(s) 79
AsuII TTCGAA 1 cut(s) 717
AvaII GGWCC 1 cut(s) 587
AxyI CCTNAGG 2 cut(s) 43, 288
BamHI GGATCC 1 cut(s) 457
BanI GGYRCC 2 cut(s) 104, 647
BanII GRGCYC 1 cut(s) 415
BauI CACGAG 1 cut(s) 364
BbsI GAAGAC 1 cut(s) 26
BbvI GCAGC 1 cut(s) 612
BccI CCATC 1 cut(s) 224
BceAI ACGGC 2 cut(s) 206, 809
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 2 cut(s) 526, 573
BcoDI GTCTC 2 cut(s) 108, 512
BfaI CTAG 1 cut(s) 732
BfmI CTRYAG 2 cut(s) 9, 309
BisI GCNGC 1 cut(s) 626
BlsI GCNGC 1 cut(s) 627
BmcAI AGTACT 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 526, 573
Bme18I GGWCC 1 cut(s) 587
BmeRI GACNNNNNGTC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 6 cut(s) 40, 106, 163, 412, 459, 649
BmrFI CCNGG 2 cut(s) 526, 573
BmsI GCATC 3 cut(s) 65, 224, 369
BpiI GAAGAC 1 cut(s) 26
BpmI CTGGAG 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 717
BpuEI CTTGAG 2 cut(s) 920, 938
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 4 cut(s) 238, 513, 572, 861
BsaXI ACNNNNNCTCC 2 cut(s) 730, 760
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 901
Bse1I ACTGG 1 cut(s) 412
Bse21I CCTNAGG 2 cut(s) 43, 288
Bse3DI GCAATG 1 cut(s) 491
BseBI CCWGG 2 cut(s) 526, 573
BseDI CCNNGG 4 cut(s) 238, 513, 572, 861
BseGI GGATG 2 cut(s) 239, 445
BseLI CCNNNNNNNGG 2 cut(s) 171, 901
BseMI GCAATG 1 cut(s) 491
BseMII CTCAG 3 cut(s) 57, 302, 905
BseNI ACTGG 1 cut(s) 412
BseRI GAGGAG 1 cut(s) 530
BseXI GCAGC 1 cut(s) 612
Bsh1236I CGCG 1 cut(s) 347
BshNI GGYRCC 2 cut(s) 104, 647
BslFI GGGAC 2 cut(s) 244, 932
BslI CCNNNNNNNGG 2 cut(s) 171, 901
BsmAI GTCTC 2 cut(s) 108, 512
BsmFI GGGAC 2 cut(s) 244, 932
Bso31I GGTCTC 1 cut(s) 108
Bsp119I TTCGAA 1 cut(s) 717
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 1 cut(s) 457
BspACI CCGC 1 cut(s) 590
BspCNI CTCAG 3 cut(s) 56, 301, 906
BspFNI CGCG 1 cut(s) 347
BspHI TCATGA 1 cut(s) 639
BspLI GGNNCC 6 cut(s) 40, 106, 163, 412, 459, 649
BspMAI CTGCAG 1 cut(s) 313
BspPI GGATC 2 cut(s) 452, 465
BspT104I TTCGAA 1 cut(s) 717
BspT107I GGYRCC 2 cut(s) 104, 647
BspTNI GGTCTC 1 cut(s) 108
BsrBI CCGCTC 1 cut(s) 592
BsrDI GCAATG 1 cut(s) 491
BsrI ACTGG 1 cut(s) 412
BssECI CCNNGG 4 cut(s) 238, 513, 572, 861
BssMI GATC 1 cut(s) 457
BssNI GRCGYC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 364
BssT1I CCWWGG 2 cut(s) 238, 861
Bst2BI CACGAG 1 cut(s) 364
Bst2UI CCWGG 2 cut(s) 526, 573
Bst4CI ACNGT 4 cut(s) 10, 25, 97, 109
BstACI GRCGYC 1 cut(s) 219
BstBI TTCGAA 1 cut(s) 717
BstDEI CTNAG 3 cut(s) 43, 288, 914
BstF5I GGATG 2 cut(s) 239, 445
BstFNI CGCG 1 cut(s) 347
BstHHI GCGC 2 cut(s) 150, 347
BstKTI GATC 1 cut(s) 460
BstMAI GTCTC 2 cut(s) 108, 512
BstMBI GATC 1 cut(s) 457
BstMWI GCNNNNNNNGC 1 cut(s) 79
BstNI CCWGG 2 cut(s) 526, 573
BstSCI CCNGG 2 cut(s) 524, 571
BstSFI CTRYAG 2 cut(s) 9, 309
BstUI CGCG 1 cut(s) 347
BstV1I GCAGC 1 cut(s) 612
BstV2I GAAGAC 1 cut(s) 26
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
Bsu36I CCTNAGG 2 cut(s) 43, 288
BtsCI GGATG 2 cut(s) 239, 445
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 318
CaiI CAGNNNCTG 1 cut(s) 281
CciI TCATGA 1 cut(s) 639
CfoI GCGC 2 cut(s) 150, 347
Cfr13I GGNCC 1 cut(s) 587
CseI GACGC 2 cut(s) 227, 246
Csp6I GTAC 3 cut(s) 6, 266, 648
CviAII CATG 4 cut(s) 60, 342, 447, 640
CviQI GTAC 3 cut(s) 6, 266, 648
DdeI CTNAG 3 cut(s) 43, 288, 914
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
DriI GACNNNNNGTC 1 cut(s) 559
Eam1105I GACNNNNNGTC 1 cut(s) 559
Eco130I CCWWGG 2 cut(s) 238, 861
Eco24I GRGCYC 1 cut(s) 415
Eco31I GGTCTC 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 587
Eco57I CTGAAG 2 cut(s) 378, 711
Eco81I CCTNAGG 2 cut(s) 43, 288
EcoRII CCWGG 2 cut(s) 524, 571
EcoT14I CCWWGG 2 cut(s) 238, 861
EcoT38I GRGCYC 1 cut(s) 415
ErhI CCWWGG 2 cut(s) 238, 861
FaeI CATG 4 cut(s) 63, 345, 450, 643
FaiI YATR 9 cut(s) 53, 61, 93, 252, 343, 448, 641, 705, 852
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 2 cut(s) 244, 932
FatI CATG 4 cut(s) 59, 341, 446, 639
Fnu4HI GCNGC 1 cut(s) 626
FokI GGATG 2 cut(s) 246, 452
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 1 cut(s) 626
FspBI CTAG 1 cut(s) 732
GlaI GCGC 2 cut(s) 149, 346
GluI GCNGC 1 cut(s) 626
GsuI CTGGAG 1 cut(s) 429
HgaI GACGC 2 cut(s) 227, 246
HhaI GCGC 2 cut(s) 150, 347
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 4 cut(s) 63, 345, 450, 643
Hin6I GCGC 2 cut(s) 148, 345
HinP1I GCGC 2 cut(s) 148, 345
HincII GTYRAC 2 cut(s) 579, 679
HindII GTYRAC 2 cut(s) 579, 679
HinfI GANTC 4 cut(s) 125, 568, 842, 885
HpaI GTTAAC 1 cut(s) 579
HphI GGTGA 1 cut(s) 79
Hpy166II GTNNAC 2 cut(s) 579, 679
Hpy188I TCNGA 6 cut(s) 420, 427, 457, 633, 841, 915
Hpy188III TCNNGA 5 cut(s) 113, 364, 640, 902, 955
Hpy8I GTNNAC 2 cut(s) 579, 679
Hpy99I CGWCG 2 cut(s) 221, 623
HpyAV CCTTC 8 cut(s) 8, 200, 310, 469, 735, 869, 905, 937
HpyCH4III ACNGT 4 cut(s) 10, 25, 97, 109
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 2 cut(s) 82, 311
HpyF10VI GCNNNNNNNGC 1 cut(s) 79
HpyF3I CTNAG 3 cut(s) 43, 288, 914
HpySE526I ACGT 1 cut(s) 681
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 4 cut(s) 63, 345, 450, 643
HspAI GCGC 2 cut(s) 148, 345
KpnI GGTACC 1 cut(s) 651
KspAI GTTAAC 1 cut(s) 579
Kzo9I GATC 1 cut(s) 457
LmnI GCTCC 3 cut(s) 38, 161, 410
Lsp1109I GCAGC 1 cut(s) 612
LweI GCATC 3 cut(s) 65, 224, 369
MaeI CTAG 1 cut(s) 732
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 3 cut(s) 31, 47, 277
MalI GATC 1 cut(s) 459
MbiI CCGCTC 1 cut(s) 592
MboI GATC 1 cut(s) 457
MboII GAAGA 3 cut(s) 31, 215, 375
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 4 cut(s) 350, 530, 610, 720
MlyI GAGTC 1 cut(s) 836
MmeI TCCRAC 1 cut(s) 864
MroXI GAANNNNTTC 2 cut(s) 174, 947
MseI TTAA 6 cut(s) 327, 377, 578, 609, 819, 961
MslI CAYNNNNRTG 1 cut(s) 368
MspR9I CCNGG 2 cut(s) 526, 573
MvaI CCWGG 2 cut(s) 526, 573
MvnI CGCG 1 cut(s) 347
MwoI GCNNNNNNNGC 1 cut(s) 79
NdeII GATC 1 cut(s) 457
NlaIII CATG 4 cut(s) 63, 345, 450, 643
NlaIV GGNNCC 6 cut(s) 40, 106, 163, 412, 459, 649
NmeAIII GCCGAG 1 cut(s) 538
NspV TTCGAA 1 cut(s) 717
OliI CACNNNNGTG 1 cut(s) 368
PagI TCATGA 1 cut(s) 639
PdmI GAANNNNTTC 2 cut(s) 174, 947
PfeI GAWTC 3 cut(s) 125, 568, 885
PkrI GCNGC 1 cut(s) 627
PleI GAGTC 1 cut(s) 836
PpsI GAGTC 1 cut(s) 836
Psp6I CCWGG 2 cut(s) 524, 571
PspGI CCWGG 2 cut(s) 524, 571
PspN4I GGNNCC 6 cut(s) 40, 106, 163, 412, 459, 649
PspPI GGNCC 1 cut(s) 587
PstI CTGCAG 1 cut(s) 313
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 1 cut(s) 457
RsaI GTAC 3 cut(s) 7, 267, 649
RsaNI GTAC 3 cut(s) 6, 266, 648
RseI CAYNNNNRTG 1 cut(s) 368
SaqAI TTAA 6 cut(s) 327, 377, 578, 609, 819, 961
SatI GCNGC 1 cut(s) 626
Sau3AI GATC 1 cut(s) 457
Sau96I GGNCC 1 cut(s) 587
ScaI AGTACT 1 cut(s) 7
SchI GAGTC 1 cut(s) 836
ScrFI CCNGG 2 cut(s) 526, 573
SduI GDGCHC 1 cut(s) 415
SetI ASST 9 cut(s) 49, 75, 244, 425, 627, 684, 733, 774, 880
SfaNI GCATC 3 cut(s) 65, 224, 369
SfcI CTRYAG 2 cut(s) 9, 309
SfuI TTCGAA 1 cut(s) 717
SinI GGWCC 1 cut(s) 587
SmiMI CAYNNNNRTG 1 cut(s) 368
SmlI CTYRAG 2 cut(s) 935, 953
SmoI CTYRAG 2 cut(s) 935, 953
Sse9I AATT 4 cut(s) 350, 530, 610, 720
SsiI CCGC 1 cut(s) 590
SspMI CTAG 1 cut(s) 732
StyD4I CCNGG 2 cut(s) 524, 571
StyI CCWWGG 2 cut(s) 238, 861
TaaI ACNGT 4 cut(s) 10, 25, 97, 109
TaiI ACGT 1 cut(s) 684
TaqI TCGA 4 cut(s) 112, 216, 621, 717
TasI AATT 4 cut(s) 350, 530, 610, 720
TatI WGTACW 2 cut(s) 5, 265
TfiI GAWTC 3 cut(s) 125, 568, 885
Tru1I TTAA 6 cut(s) 327, 377, 578, 609, 819, 961
Tru9I TTAA 6 cut(s) 327, 377, 578, 609, 819, 961
TscAI CASTG 1 cut(s) 318
TseI GCWGC 1 cut(s) 625
TspDTI ATGAA 5 cut(s) 167, 239, 456, 628, 837
TspGWI ACGGA 2 cut(s) 174, 607
TspRI CASTG 1 cut(s) 318
VpaK11BI GGWCC 1 cut(s) 587
XapI RAATTY 3 cut(s) 350, 530, 720
XmnI GAANNNNTTC 2 cut(s) 174, 947
XspI CTAG 1 cut(s) 732
ZrmI AGTACT 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.