Prupe.2G021000_v2.0.a1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0000709
Physical Location & Seq
Reverse (-)
2068732 .. 2071591
2860 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G021000.1

Sequence Viewer

Length: 975 bp
ATGAACGAAACAAGGAAGGTGGTGTGCGTAACAGGAGCATCTGGTTTCATAGCATCCTGGCTGGTGAAGCTCTTACTGGAACGAGCTTATATTGTCAAAGCAACCGTCCGTGACCCAAATGACCCGAAGAAAACAGAACACTTATTGGCACTTGAGGGGGCAAAAGAAAGGCTCCATTTGTTCAAAGCAGATTTGTTAGAAGAAGGATCTTTTGATGCTGTTGTTGATGGATGTGAGGGTGTTTTTCATACAGCATCCCCTGTAATACTTTCATCCACTGACCCACAGACAGAATTAATTGACCCTGCAGTGAAGGGAACCCTTAATGTTCTAAAATCGTGCGTAAAATTCGCTACCGTCAAGAGGGTAGTTTTAACATCTTCTACGGCTGCAGTCATATTCAATGGAAGACCATTGACCCCTGATGTGGTGGTTGATGAAACATGGTTTTCAGATCCTTTTGTTTGTGAGAATTTTAAGAAATGGTATGTTCTTTCAAAAACTTTAGCTGAGGAAGCGGCTTGGAAATTTGCTGAAGGAAATGGGATTGACTTGGTTACCATAAATCCATCCTTTGTGATTGGTCCACTCTTACAACCAAGCCTTAACATCACCGTGGAGATGATTCTCAACTTCAAAAACGATGTTGAAGATGTAAACAGTTCTATTTATTCATGTAGTGATGTTAGAGATGTTGCTGCAGCTCATGTTCAAGCATTTGAGGTTCCTTCAGCTAATGGAAGATATTGTTTAGTTGGACATGTTACACCCATGGCCAAGGTTCTGAAGATTTTACATGAACTTCATCCCTCTTTGTGCCCACCTGAAAAATATGAGGAGGCCAATCCTTCTGAACCCACATATCAAGTATCACAGGAAAAAGCAAAAAGTTTGGGAGTTAATTTCCTTCCACTGGAAGTAAGTCTTAGTGACACTGTTGAAAGCCTCAAGGAGAAGGGGTTCCTCAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

325

Amino Acids

35.55

Weight (kDa)

5.29

Isoelectric Point (pI)

24.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 518
AclWI GGATC 2 cut(s) 214, 449
AcoI YGGCCR 1 cut(s) 774
AcsI RAATTY 3 cut(s) 347, 472, 527
AcuI CTGAAG 3 cut(s) 555, 714, 806
AfiI CCNNNNNNNGG 3 cut(s) 363, 427, 913
AflIII ACRYGT 1 cut(s) 760
AgsI TTSAA 7 cut(s) 184, 403, 498, 637, 650, 713, 941
AjnI CCWGG 1 cut(s) 56
AloI GAACNNNNNNTCC 1 cut(s) 944
AluBI AGCT 5 cut(s) 70, 86, 509, 704, 734
AluI AGCT 5 cut(s) 70, 86, 509, 704, 734
AlwI GGATC 2 cut(s) 214, 449
AoxI GGCC 2 cut(s) 774, 840
ApeKI GCWGC 3 cut(s) 389, 698, 701
ApoI RAATTY 3 cut(s) 347, 472, 527
ArsI GACNNNNNNTTYG 2 cut(s) 90, 122
AseI ATTAAT 1 cut(s) 296
Asp700I GAANNNNTTC 1 cut(s) 959
AspS9I GGNCC 1 cut(s) 584
AsuHPI GGTGA 2 cut(s) 76, 604
AvaII GGWCC 1 cut(s) 584
BaeGI GKGCMC 1 cut(s) 821
BalI TGGCCA 1 cut(s) 776
BbsI GAAGAC 1 cut(s) 415
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 3 cut(s) 376, 685, 713
BccI CCATC 2 cut(s) 221, 577
BceAI ACGGC 1 cut(s) 402
BciT130I CCWGG 1 cut(s) 58
BfmI CTRYAG 3 cut(s) 306, 390, 699
BisI GCNGC 4 cut(s) 390, 519, 699, 702
BlsI GCNGC 4 cut(s) 391, 520, 700, 703
Bme1390I CCNGG 1 cut(s) 58
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 1 cut(s) 584
BmiI GGNNCC 4 cut(s) 173, 319, 726, 962
BmrFI CCNGG 1 cut(s) 58
BmsI GCATC 4 cut(s) 47, 62, 205, 263
BpiI GAAGAC 1 cut(s) 415
Bpu10I CCTNAGC 1 cut(s) 510
BpuEI CTTGAG 3 cut(s) 173, 932, 950
BsaJI CCNNGG 3 cut(s) 615, 771, 777
BsaXI ACNNNNNCTCC 2 cut(s) 944, 974
Bsc4I CCNNNNNNNGG 3 cut(s) 363, 427, 913
Bse1I ACTGG 2 cut(s) 81, 918
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 3 cut(s) 615, 771, 777
BseGI GGATG 6 cut(s) 53, 236, 254, 272, 569, 805
BseLI CCNNNNNNNGG 3 cut(s) 363, 427, 913
BseMII CTCAG 1 cut(s) 501
BseNI ACTGG 2 cut(s) 81, 918
BseRI GAGGAG 1 cut(s) 851
BseSI GKGCMC 1 cut(s) 821
BseXI GCAGC 3 cut(s) 376, 685, 713
BshFI GGCC 2 cut(s) 776, 842
BslI CCNNNNNNNGG 3 cut(s) 363, 427, 913
BsnI GGCC 2 cut(s) 776, 842
Bsp1286I GDGCHC 1 cut(s) 821
Bsp143I GATC 2 cut(s) 206, 454
Bsp19I CCATGG 1 cut(s) 771
BspACI CCGC 1 cut(s) 518
BspANI GGCC 2 cut(s) 776, 842
BspCNI CTCAG 1 cut(s) 502
BspLI GGNNCC 4 cut(s) 173, 319, 726, 962
BspMAI CTGCAG 3 cut(s) 310, 394, 703
BspPI GGATC 2 cut(s) 214, 449
BsrI ACTGG 2 cut(s) 81, 918
BssECI CCNNGG 3 cut(s) 615, 771, 777
BssMI GATC 2 cut(s) 206, 454
BssT1I CCWWGG 2 cut(s) 771, 777
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 5 cut(s) 106, 358, 616, 662, 937
BstDEI CTNAG 2 cut(s) 510, 926
BstDSI CCRYGG 2 cut(s) 615, 771
BstEII GGTNACC 1 cut(s) 556
BstF5I GGATG 6 cut(s) 53, 236, 254, 272, 569, 805
BstKTI GATC 2 cut(s) 209, 457
BstMBI GATC 2 cut(s) 206, 454
BstMWI GCNNNNNNNGC 2 cut(s) 67, 515
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 764
BstPI GGTNACC 1 cut(s) 556
BstSCI CCNGG 1 cut(s) 56
BstSFI CTRYAG 3 cut(s) 306, 390, 699
BstSLI GKGCMC 1 cut(s) 821
BstV1I GCAGC 3 cut(s) 376, 685, 713
BstV2I GAAGAC 1 cut(s) 415
BstX2I RGATCY 2 cut(s) 206, 454
BstYI RGATCY 2 cut(s) 206, 454
BsuRI GGCC 2 cut(s) 776, 842
BtgI CCRYGG 2 cut(s) 615, 771
BtsCI GGATG 6 cut(s) 53, 236, 254, 272, 569, 805
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 4 cut(s) 276, 315, 911, 933
Cfr13I GGNCC 1 cut(s) 584
CspCI CAANNNNNGTGG 1 cut(s) 35
CviAII CATG 6 cut(s) 444, 675, 707, 761, 772, 797
DdeI CTNAG 2 cut(s) 510, 926
DpnI GATC 2 cut(s) 208, 456
DpnII GATC 2 cut(s) 206, 454
EaeI YGGCCR 1 cut(s) 774
Eco130I CCWWGG 2 cut(s) 771, 777
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 3 cut(s) 555, 714, 806
Eco91I GGTNACC 1 cut(s) 556
EcoO65I GGTNACC 1 cut(s) 556
EcoRII CCWGG 1 cut(s) 56
EcoT14I CCWWGG 2 cut(s) 771, 777
ErhI CCWWGG 2 cut(s) 771, 777
FaeI CATG 6 cut(s) 447, 678, 710, 764, 775, 800
FalI AAGNNNNNCTT 2 cut(s) 909, 941
FatI CATG 6 cut(s) 443, 674, 706, 760, 771, 796
Fnu4HI GCNGC 4 cut(s) 390, 519, 699, 702
FokI GGATG 6 cut(s) 40, 241, 243, 259, 556, 792
Fsp4HI GCNGC 4 cut(s) 390, 519, 699, 702
GluI GCNGC 4 cut(s) 390, 519, 699, 702
HaeIII GGCC 2 cut(s) 776, 842
Hin1II CATG 6 cut(s) 447, 678, 710, 764, 775, 800
HinfI GANTC 1 cut(s) 625
HphI GGTGA 2 cut(s) 76, 604
Hpy166II GTNNAC 2 cut(s) 587, 658
Hpy188I TCNGA 3 cut(s) 454, 786, 853
Hpy188III TCNNGA 1 cut(s) 361
Hpy8I GTNNAC 2 cut(s) 587, 658
HpyAV CCTTC 8 cut(s) 10, 197, 307, 530, 738, 858, 917, 949
HpyCH4III ACNGT 5 cut(s) 106, 358, 616, 662, 937
HpyCH4V TGCA 3 cut(s) 308, 392, 701
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 515
HpyF3I CTNAG 2 cut(s) 510, 926
Hsp92II CATG 6 cut(s) 447, 678, 710, 764, 775, 800
Kzo9I GATC 2 cut(s) 206, 454
LmnI GCTCC 2 cut(s) 35, 177
Lsp1109I GCAGC 3 cut(s) 376, 685, 713
LweI GCATC 4 cut(s) 47, 62, 205, 263
MaeIII GTNAC 5 cut(s) 28, 110, 556, 763, 929
MalI GATC 2 cut(s) 208, 456
MboI GATC 2 cut(s) 206, 454
MboII GAAGA 7 cut(s) 139, 212, 372, 420, 662, 753, 799
MflI RGATCY 2 cut(s) 206, 454
MhlI GDGCHC 1 cut(s) 821
MlsI TGGCCA 1 cut(s) 776
MluCI AATT 6 cut(s) 293, 297, 347, 472, 527, 901
MluNI TGGCCA 1 cut(s) 776
MmeI TCCRAC 1 cut(s) 736
Mox20I TGGCCA 1 cut(s) 776
MroXI GAANNNNTTC 1 cut(s) 959
MscI TGGCCA 1 cut(s) 776
MseI TTAA 6 cut(s) 296, 324, 374, 477, 606, 900
MslI CAYNNNNRTG 1 cut(s) 614
Msp20I TGGCCA 1 cut(s) 776
MspR9I CCNGG 1 cut(s) 58
MvaI CCWGG 1 cut(s) 58
MwoI GCNNNNNNNGC 2 cut(s) 67, 515
NcoI CCATGG 1 cut(s) 771
NdeII GATC 2 cut(s) 206, 454
NlaIII CATG 6 cut(s) 447, 678, 710, 764, 775, 800
NlaIV GGNNCC 4 cut(s) 173, 319, 726, 962
NmuCI GTSAC 2 cut(s) 110, 929
NspI RCATGY 1 cut(s) 764
PciI ACATGT 1 cut(s) 760
PdmI GAANNNNTTC 1 cut(s) 959
PfeI GAWTC 1 cut(s) 625
PkrI GCNGC 4 cut(s) 391, 520, 700, 703
PscI ACATGT 1 cut(s) 760
PshBI ATTAAT 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 56
PspEI GGTNACC 1 cut(s) 556
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 4 cut(s) 173, 319, 726, 962
PspPI GGNCC 1 cut(s) 584
PstI CTGCAG 3 cut(s) 310, 394, 703
PsuI RGATCY 2 cut(s) 206, 454
RseI CAYNNNNRTG 1 cut(s) 614
SaqAI TTAA 6 cut(s) 296, 324, 374, 477, 606, 900
SatI GCNGC 4 cut(s) 390, 519, 699, 702
Sau3AI GATC 2 cut(s) 206, 454
Sau96I GGNCC 1 cut(s) 584
ScrFI CCNGG 1 cut(s) 58
SduI GDGCHC 1 cut(s) 821
SfaNI GCATC 4 cut(s) 47, 62, 205, 263
SfcI CTRYAG 3 cut(s) 306, 390, 699
SinI GGWCC 1 cut(s) 584
SmiMI CAYNNNNRTG 1 cut(s) 614
SmlI CTYRAG 3 cut(s) 152, 947, 965
SmoI CTYRAG 3 cut(s) 152, 947, 965
Sse9I AATT 6 cut(s) 293, 297, 347, 472, 527, 901
SsiI CCGC 1 cut(s) 518
StyD4I CCNGG 1 cut(s) 56
StyI CCWWGG 2 cut(s) 771, 777
TaaI ACNGT 5 cut(s) 106, 358, 616, 662, 937
TasI AATT 6 cut(s) 293, 297, 347, 472, 527, 901
TauI GCSGC 1 cut(s) 521
TfiI GAWTC 1 cut(s) 625
Tru1I TTAA 6 cut(s) 296, 324, 374, 477, 606, 900
Tru9I TTAA 6 cut(s) 296, 324, 374, 477, 606, 900
TscAI CASTG 4 cut(s) 283, 315, 918, 940
TseFI GTSAC 2 cut(s) 110, 929
TseI GCWGC 3 cut(s) 389, 698, 701
Tsp45I GTSAC 2 cut(s) 110, 929
TspDTI ATGAA 8 cut(s) 17, 37, 236, 261, 453, 663, 794, 813
TspGWI ACGGA 1 cut(s) 98
TspRI CASTG 4 cut(s) 283, 315, 918, 940
VpaK11BI GGWCC 1 cut(s) 584
VspI ATTAAT 1 cut(s) 296
XapI RAATTY 3 cut(s) 347, 472, 527
XceI RCATGY 1 cut(s) 764
XmnI GAANNNNTTC 1 cut(s) 959
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.