MD02G1305300.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000709
Physical Location & Seq
Forward (+)
35844401 .. 35846552
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1305300.v1.1.491

Sequence Viewer

Length: 963 bp
ATGAGTGGGGGAACGAAGGTTGTGTGTGTAACAGGAGCCTCCGGCTACATAGGCTCGTGGCTTGTGAAGCTCTTGCTACAAAGAGGTTATACTGTCAGAGCTACCGTTCGCGACCCAAATGATCCAAAGAAAACAGAGCACTTGCTCTCACTTGATGGAGCAAAAGAAAGGCTTCATTTGTTCAAGGCAGGTTTATTAGAAGAAGGAGCTTTCGACCCCGTAATTGATGGATGCGTTGGTGTTTTTCATACAGCATCGCCTGTACAACTTTCAGCCACTGACCCACAGGCAGAAATAGTTGAGCCTGCAGTGAAGGGAACTCTGAATGTTCTAAAATCATGTGCGAAATTTCCCACTGTCAAGAGAGTGGTTTTAACATCTTCCATGTCTTCAGTATCGTTTAGTAGAACACCGATAACTTCTGATATGGTTTTGGATGAAACATGGTACTCAGATCCACTTGTTTGTGAGCAGAACCAGAGATGGTATCCGCTCTCCAAAATTTTAGCTGAGGAGGCTGCCTGGAAATTTGCAAAAGAAAATGGGATCGACTTGGTGTCGATGAATCCAGGGATTGTGATTGGTCCGCTCTTACAGCCAACTCTTAATGGCTCCGTGGAGCTGATTGCAAATATCATAAGTGGTATCCAAACACCATTTCTAAATTATACATTTGTAGACGTTAGAGATGTTGCCTCTGCCCATATTCAAGCATTCGAAGTTCCTTCAGCTAATGGCAGATATTGTTTAGTTGGCCAAGTAGCTGACGGCCCCGATACTCTACAGATTTTACGACAACTTTACCCCACTTTGTGCCTTCCTGAGTTTGGCAATCCTTCTGACTCAAAGTATCAAGTGTCAAAGGAGAAAGCGAAAAGTTTGGGAATTAATTTCCTTCCTCTGGAAGCAAGTCTAAGGGACACTATTGAAAGCCTCAAGGAGAAGGGCTTCCTCAATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

321

Amino Acids

34.83

Weight (kDa)

5.68

Isoelectric Point (pI)

33.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 100 2.4e-08 NmrA-like family
Epimerase PF01370 8 - 244 8.4e-26 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 239 1.7e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 10 - 218 1.1e-11 Male sterility protein
GDP_Man_Dehyd PF16363 10 - 189 4.3e-12 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 131 8.5e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000709)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01511 FvH4_7g01511 FvH4_7g01511 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01520 FvH4_7g01560 FvH4_7g01560
malus_domestica MD02G1303400.v1.1 MD02G1303800.v1.1 MD02G1303900.v1.1 MD02G1304200.v1.1 MD02G1304400.v1.1 MD02G1304900.v1.1 MD02G1305300.v1.1 MD02G1305400.v1.1 MD04G1006100.v1.1 MD07G1020300.v1.1 MD07G1020900.v1.1 MD07G1021200.v1.1 MD07G1021500.v1.1
prunus_persica Prupe.2G020700_v2.0.a1 Prupe.2G021000_v2.0.a1 Prupe.2G021100_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021300_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1 Prupe.2G021500_v2.0.a1
pyrus_communis pycom02g25550 pycom02g25560 pycom02g25570 pycom02g25590 pycom07g01560 pycom07g01570 pycom07g01630
rosa_chinensis RchiOBHm_Chr1g0317371 RchiOBHm_Chr1g0317421 RchiOBHm_Chr1g0317441 RchiOBHm_Chr1g0317501 RchiOBHm_Chr1g0317581 RchiOBHm_Chr1g0317591
rosa_laevigata RLG00000030523 RLG00000030595 RLG00000030598
rosa_multiflora Rmu_sc0001403.1_g000012 Rmu_sc0001403.1_g000037 Rmu_sc0003374.1_g000001 Rmu_sc0003374.1_g000006 Rmu_sc0009583.1_g000010
rosa_roxburghii Rroxscaffold_4G00330280 Rroxscaffold_4G00330300 Rroxscaffold_4G00330420 Rroxscaffold_4G00330450
rosa_rugosa Rorug01G0016100 Rorug01G0016200 Rorug01G0016200 Rorug01G0016300 Rorug01G0016400
rosa_samantha Rh1BG023400 Rh1BG023800 Rh1BG024400 Rh1DG044600 Rh1DG044900 Rh1DG045100 Rh1DG045200 Rh1DG045400 Rh1DG045600
rosa_wichuraiana Rw0G002050 Rw1G001940 Rw1G001950 Rw1G002000 Rw1G002030

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 179
AccBSI CCGCTC 2 cut(s) 493, 589
AccI GTMKAC 1 cut(s) 678
AccII CGCG 1 cut(s) 111
AciI CCGC 2 cut(s) 491, 587
AclWI GGATC 3 cut(s) 116, 449, 554
AcoI YGGCCR 1 cut(s) 754
AcsI RAATTY 3 cut(s) 347, 501, 527
AcuI CTGAAG 2 cut(s) 375, 711
AdeI CACNNNGTG 1 cut(s) 813
AfaI GTAC 2 cut(s) 264, 449
AfiI CCNNNNNNNGG 2 cut(s) 827, 901
AgsI TTSAA 3 cut(s) 184, 710, 929
AhdI GACNNNNNGTC 1 cut(s) 556
AjnI CCWGG 2 cut(s) 521, 568
AjuI GAANNNNNNNTTGG 2 cut(s) 642, 674
AluBI AGCT 7 cut(s) 70, 101, 209, 509, 622, 731, 764
AluI AGCT 7 cut(s) 70, 101, 209, 509, 622, 731, 764
Alw21I GWGCWC 1 cut(s) 141
AlwI GGATC 3 cut(s) 116, 449, 554
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 2 cut(s) 754, 769
ApeKI GCWGC 1 cut(s) 518
ApoI RAATTY 3 cut(s) 347, 501, 527
AseI ATTAAT 1 cut(s) 888
Asp700I GAANNNNTTC 2 cut(s) 171, 947
AspS9I GGNCC 2 cut(s) 584, 770
AsuII TTCGAA 1 cut(s) 717
AvaII GGWCC 1 cut(s) 584
BalI TGGCCA 1 cut(s) 756
BauI CACGAG 1 cut(s) 55
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 141
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 1 cut(s) 505
BccI CCATC 3 cut(s) 149, 221, 477
BceAI ACGGC 1 cut(s) 784
BciT130I CCWGG 2 cut(s) 523, 570
BciVI GTATCC 2 cut(s) 498, 656
BfmI CTRYAG 2 cut(s) 306, 782
BfuAI ACCTGC 1 cut(s) 179
BfuI GTATCC 2 cut(s) 498, 656
BisI GCNGC 1 cut(s) 519
BlsI GCNGC 1 cut(s) 520
Bme1390I CCNGG 2 cut(s) 523, 570
Bme18I GGWCC 1 cut(s) 584
BmeRI GACNNNNNGTC 1 cut(s) 556
BmgT120I GGNCC 2 cut(s) 584, 770
BmiI GGNNCC 3 cut(s) 37, 613, 772
BmrFI CCNGG 2 cut(s) 523, 570
BmsI GCATC 2 cut(s) 221, 263
BpiI GAAGAC 1 cut(s) 381
Bpu10I CCTNAGC 1 cut(s) 510
Bpu14I TTCGAA 1 cut(s) 717
BpuEI CTTGAG 1 cut(s) 920
BsaJI CCNNGG 2 cut(s) 569, 615
Bsc4I CCNNNNNNNGG 2 cut(s) 827, 901
BseBI CCWGG 2 cut(s) 523, 570
BseDI CCNNGG 2 cut(s) 569, 615
BseGI GGATG 2 cut(s) 236, 442
BseLI CCNNNNNNNGG 2 cut(s) 827, 901
BseMII CTCAG 3 cut(s) 465, 501, 813
BseRI GAGGAG 1 cut(s) 527
BseXI GCAGC 1 cut(s) 505
Bsh1236I CGCG 1 cut(s) 111
BshFI GGCC 2 cut(s) 756, 771
BsiHKAI GWGCWC 1 cut(s) 141
BsiSI CCGG 1 cut(s) 42
BslFI GGGAC 1 cut(s) 932
BslI CCNNNNNNNGG 2 cut(s) 827, 901
BsmFI GGGAC 1 cut(s) 932
BsmI GAATGC 1 cut(s) 713
BsnI GGCC 2 cut(s) 756, 771
Bsp119I TTCGAA 1 cut(s) 717
Bsp1286I GDGCHC 1 cut(s) 141
Bsp1407I TGTACA 1 cut(s) 262
Bsp143I GATC 3 cut(s) 121, 454, 546
Bsp68I TCGCGA 1 cut(s) 111
BspACI CCGC 2 cut(s) 491, 587
BspANI GGCC 2 cut(s) 756, 771
BspCNI CTCAG 3 cut(s) 464, 502, 814
BspFNI CGCG 1 cut(s) 111
BspLI GGNNCC 3 cut(s) 37, 613, 772
BspMAI CTGCAG 1 cut(s) 310
BspMI ACCTGC 1 cut(s) 179
BspPI GGATC 3 cut(s) 116, 449, 554
BspT104I TTCGAA 1 cut(s) 717
BsrBI CCGCTC 2 cut(s) 493, 589
BsrGI TGTACA 1 cut(s) 262
BssECI CCNNGG 2 cut(s) 569, 615
BssMI GATC 3 cut(s) 121, 454, 546
BssSI CACGAG 1 cut(s) 55
Bst2BI CACGAG 1 cut(s) 55
Bst2UI CCWGG 2 cut(s) 523, 570
Bst4CI ACNGT 3 cut(s) 94, 106, 358
BstAUI TGTACA 1 cut(s) 262
BstBI TTCGAA 1 cut(s) 717
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 4 cut(s) 451, 510, 822, 914
BstDSI CCRYGG 1 cut(s) 615
BstF5I GGATG 2 cut(s) 236, 442
BstFNI CGCG 1 cut(s) 111
BstKTI GATC 3 cut(s) 124, 457, 549
BstMBI GATC 3 cut(s) 121, 454, 546
BstMWI GCNNNNNNNGC 4 cut(s) 51, 67, 515, 595
BstNI CCWGG 2 cut(s) 523, 570
BstSCI CCNGG 2 cut(s) 521, 568
BstSFI CTRYAG 2 cut(s) 306, 782
BstUI CGCG 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 505
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
BsuI GTATCC 2 cut(s) 498, 656
BsuRI GGCC 2 cut(s) 756, 771
BtgI CCRYGG 1 cut(s) 615
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 2 cut(s) 236, 442
BtsI GCAGTG 1 cut(s) 315
BtsIMutI CAGTG 3 cut(s) 276, 315, 354
BtuMI TCGCGA 1 cut(s) 111
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 306
CaiI CAGNNNCTG 1 cut(s) 278
Cfr13I GGNCC 2 cut(s) 584, 770
Csp6I GTAC 2 cut(s) 263, 448
CviAII CATG 3 cut(s) 339, 385, 444
CviQI GTAC 2 cut(s) 263, 448
DdeI CTNAG 4 cut(s) 451, 510, 822, 914
DpnI GATC 3 cut(s) 123, 456, 548
DpnII GATC 3 cut(s) 121, 454, 546
DraIII CACNNNGTG 1 cut(s) 813
DriI GACNNNNNGTC 1 cut(s) 556
EaeI YGGCCR 1 cut(s) 754
Eam1105I GACNNNNNGTC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 2 cut(s) 375, 711
EcoRII CCWGG 2 cut(s) 521, 568
FaeI CATG 3 cut(s) 342, 388, 447
FalI AAGNNNNNCTT 2 cut(s) 156, 188
FaqI GGGAC 1 cut(s) 932
FatI CATG 3 cut(s) 338, 384, 443
FblI GTMKAC 1 cut(s) 678
Fnu4HI GCNGC 1 cut(s) 519
FokI GGATG 2 cut(s) 243, 449
Fsp4HI GCNGC 1 cut(s) 519
GluI GCNGC 1 cut(s) 519
HaeIII GGCC 2 cut(s) 756, 771
HapII CCGG 1 cut(s) 42
Hin1II CATG 3 cut(s) 342, 388, 447
HinfI GANTC 2 cut(s) 565, 842
HpaII CCGG 1 cut(s) 42
Hpy166II GTNNAC 1 cut(s) 679
Hpy188I TCNGA 6 cut(s) 98, 324, 424, 454, 841, 962
Hpy188III TCNNGA 4 cut(s) 110, 361, 821, 902
Hpy8I GTNNAC 1 cut(s) 679
HpyAV CCTTC 8 cut(s) 10, 197, 307, 735, 827, 846, 905, 937
HpyCH4III ACNGT 3 cut(s) 94, 106, 358
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 3 cut(s) 308, 533, 629
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 67, 515, 595
HpyF3I CTNAG 4 cut(s) 451, 510, 822, 914
HpySE526I ACGT 1 cut(s) 681
Hsp92II CATG 3 cut(s) 342, 388, 447
Kzo9I GATC 3 cut(s) 121, 454, 546
LmnI GCTCC 5 cut(s) 35, 158, 206, 617, 619
Lsp1109I GCAGC 1 cut(s) 505
LweI GCATC 2 cut(s) 221, 263
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 1 cut(s) 28
MalI GATC 3 cut(s) 123, 456, 548
MbiI CCGCTC 2 cut(s) 493, 589
MboI GATC 3 cut(s) 121, 454, 546
MboII GAAGA 3 cut(s) 212, 372, 381
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 1 cut(s) 141
MlsI TGGCCA 1 cut(s) 756
MluCI AATT 7 cut(s) 222, 347, 501, 527, 664, 885, 889
MluNI TGGCCA 1 cut(s) 756
MlyI GAGTC 1 cut(s) 836
MnlI CCTC 8 cut(s) 49, 77, 505, 508, 706, 909, 944, 962
Mox20I TGGCCA 1 cut(s) 756
MroXI GAANNNNTTC 2 cut(s) 171, 947
MscI TGGCCA 1 cut(s) 756
MseI TTAA 3 cut(s) 374, 606, 888
Msp20I TGGCCA 1 cut(s) 756
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 2 cut(s) 523, 570
Mva1269I GAATGC 1 cut(s) 713
MvaI CCWGG 2 cut(s) 523, 570
MvnI CGCG 1 cut(s) 111
MwoI GCNNNNNNNGC 4 cut(s) 51, 67, 515, 595
NdeII GATC 3 cut(s) 121, 454, 546
NlaIII CATG 3 cut(s) 342, 388, 447
NlaIV GGNNCC 3 cut(s) 37, 613, 772
NruI TCGCGA 1 cut(s) 111
NspV TTCGAA 1 cut(s) 717
PctI GAATGC 1 cut(s) 713
PdmI GAANNNNTTC 2 cut(s) 171, 947
PfeI GAWTC 1 cut(s) 565
PkrI GCNGC 1 cut(s) 520
PleI GAGTC 1 cut(s) 836
PpsI GAGTC 1 cut(s) 836
PshBI ATTAAT 1 cut(s) 888
Psp6I CCWGG 2 cut(s) 521, 568
PspGI CCWGG 2 cut(s) 521, 568
PspN4I GGNNCC 3 cut(s) 37, 613, 772
PspPI GGNCC 2 cut(s) 584, 770
PstI CTGCAG 1 cut(s) 310
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 1 cut(s) 454
RruI TCGCGA 1 cut(s) 111
RsaI GTAC 2 cut(s) 264, 449
RsaNI GTAC 2 cut(s) 263, 448
SaqAI TTAA 3 cut(s) 374, 606, 888
SatI GCNGC 1 cut(s) 519
Sau3AI GATC 3 cut(s) 121, 454, 546
Sau96I GGNCC 2 cut(s) 584, 770
SchI GAGTC 1 cut(s) 836
ScrFI CCNGG 2 cut(s) 523, 570
SduI GDGCHC 1 cut(s) 141
SfaNI GCATC 2 cut(s) 221, 263
SfcI CTRYAG 2 cut(s) 306, 782
SfuI TTCGAA 1 cut(s) 717
SinI GGWCC 1 cut(s) 584
SmlI CTYRAG 1 cut(s) 935
SmoI CTYRAG 1 cut(s) 935
Sse9I AATT 7 cut(s) 222, 347, 501, 527, 664, 885, 889
SsiI CCGC 2 cut(s) 491, 587
StyD4I CCNGG 2 cut(s) 521, 568
TaaI ACNGT 3 cut(s) 94, 106, 358
TaiI ACGT 1 cut(s) 684
TaqI TCGA 4 cut(s) 213, 549, 560, 717
TasI AATT 7 cut(s) 222, 347, 501, 527, 664, 885, 889
TatI WGTACW 1 cut(s) 262
TfiI GAWTC 1 cut(s) 565
Tru1I TTAA 3 cut(s) 374, 606, 888
Tru9I TTAA 3 cut(s) 374, 606, 888
TscAI CASTG 3 cut(s) 283, 315, 361
TseI GCWGC 1 cut(s) 518
TspDTI ATGAA 4 cut(s) 164, 236, 453, 578
TspGWI ACGGA 1 cut(s) 604
TspRI CASTG 3 cut(s) 283, 315, 361
VpaK11BI GGWCC 1 cut(s) 584
VspI ATTAAT 1 cut(s) 888
XapI RAATTY 3 cut(s) 347, 501, 527
XmiI GTMKAC 1 cut(s) 678
XmnI GAANNNNTTC 2 cut(s) 171, 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.