FvH4_7g01563

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000539
Physical Location & Seq
Reverse (-)
1967201 .. 1969998
2798 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01563.t2

Sequence Viewer

Length: 672 bp
ATGAGATGCGGAGGAGGGAAGGTTGTGTGTGTGACAGGAGCATCTGGGTTCATAGCTTCATGGCTGGTCAAGCTCTTGTTGCAACGAGGTTATACTGTTAAAGCCACTGTACGGAACCCAAATGATCAAAAGAAAACAGAACACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGATGAAGGTTCTTTTGATTCTGTAGTTGATGGTTGTGAATGTGTTTTTCATAAGCCATCACCTGAGACACCTGTAATAGTTCCAGCCACTGACCCACAGGCAGAATTAATTGACCCTGCTTTGAAGGGTACTCTTAATGTCCTTGGATCGTGTGTGAAGATTTCGTCTGTCAAAAGGGTGGTTATCACATCCTCTATGGCAGCAGTTGCATACAATGGAAAACATTTAGCTGATGATGTAACCATTGATGAATCCTGGTTTTCAGATCCTGCTTTTTGTGAAAAGACAAAGCTTTGGTATCAGCTTTCAAAGACATTAGCTGAGGAGGCTGCTTGGAAGTTTGCAAAAGAGAAAGGAATTGATGTTATTACAATAAATCCGGGATGGGTGATCGGCCCTCTCTTACAGCCAACTCTGAACTTTAGTGTGGAACCAGTTCTTAAACTCGTAAATGTACAATCATTTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

224

Amino Acids

24.26

Weight (kDa)

6.43

Isoelectric Point (pI)

27.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 85 1.3e-08 NmrA-like family
Epimerase PF01370 9 - 201 6.9e-20 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 179 3e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 11 - 203 9.6e-12 Male sterility protein
GDP_Man_Dehyd PF16363 11 - 191 2.5e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 137 1.9e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 9
AclWI GGATC 2 cut(s) 355, 461
AfaI GTAC 3 cut(s) 111, 331, 657
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 3 cut(s) 187, 325, 510
AjnI CCWGG 1 cut(s) 455
AluBI AGCT 6 cut(s) 56, 73, 431, 493, 505, 521
AluI AGCT 6 cut(s) 56, 73, 431, 493, 505, 521
Alw26I GTCTC 1 cut(s) 260
AlwI GGATC 2 cut(s) 355, 461
AlwNI CAGNNNCTG 2 cut(s) 290, 470
AoxI GGCC 1 cut(s) 595
ApeKI GCWGC 2 cut(s) 401, 530
AseI ATTAAT 1 cut(s) 308
Asp700I GAANNNNTTC 2 cut(s) 174, 636
AspS9I GGNCC 1 cut(s) 596
AsuC2I CCSGG 1 cut(s) 582
AsuHPI GGTGA 2 cut(s) 252, 601
BbvCI CCTCAGC 1 cut(s) 522
BbvI GCAGC 2 cut(s) 413, 517
BccI CCATC 4 cut(s) 152, 224, 265, 579
BciT130I CCWGG 1 cut(s) 457
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 1 cut(s) 582
BcoDI GTCTC 1 cut(s) 260
BfmI CTRYAG 1 cut(s) 222
BisI GCNGC 2 cut(s) 402, 531
BlsI GCNGC 2 cut(s) 403, 532
Bme1390I CCNGG 2 cut(s) 457, 582
BmgT120I GGNCC 1 cut(s) 596
BmiI GGNNCC 2 cut(s) 116, 633
BmrFI CCNGG 2 cut(s) 457, 582
BmsI GCATC 1 cut(s) 50
Bpu10I CCTNAGC 1 cut(s) 522
BpuMI CCSGG 1 cut(s) 582
BsaJI CCNNGG 1 cut(s) 343
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 635
BseBI CCWGG 1 cut(s) 457
BseDI CCNNGG 1 cut(s) 343
BseGI GGATG 2 cut(s) 389, 590
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 2 cut(s) 255, 513
BseNI ACTGG 1 cut(s) 635
BseRI GAGGAG 2 cut(s) 27, 539
BseXI GCAGC 2 cut(s) 413, 517
BshFI GGCC 1 cut(s) 597
BsiSI CCGG 1 cut(s) 581
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 260
BsnI GGCC 1 cut(s) 597
Bsp1407I TGTACA 1 cut(s) 655
Bsp143I GATC 4 cut(s) 124, 347, 466, 591
BspACI CCGC 1 cut(s) 9
BspANI GGCC 1 cut(s) 597
BspCNI CTCAG 2 cut(s) 256, 514
BspLI GGNNCC 2 cut(s) 116, 633
BspPI GGATC 2 cut(s) 355, 461
BsrGI TGTACA 1 cut(s) 655
BsrI ACTGG 1 cut(s) 635
BssECI CCNNGG 1 cut(s) 343
BssMI GATC 4 cut(s) 124, 347, 466, 591
BssT1I CCWWGG 1 cut(s) 343
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 2 cut(s) 97, 109
BstAPI GCANNNNNTGC 1 cut(s) 407
BstAUI TGTACA 1 cut(s) 655
BstDEI CTNAG 2 cut(s) 264, 522
BstF5I GGATG 2 cut(s) 389, 590
BstKTI GATC 4 cut(s) 127, 350, 469, 594
BstMAI GTCTC 1 cut(s) 260
BstMBI GATC 4 cut(s) 124, 347, 466, 591
BstMWI GCNNNNNNNGC 4 cut(s) 70, 79, 407, 527
BstNI CCWGG 1 cut(s) 457
BstSCI CCNGG 2 cut(s) 455, 580
BstSFI CTRYAG 1 cut(s) 222
BstV1I GCAGC 2 cut(s) 413, 517
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 1 cut(s) 597
BtsCI GGATG 2 cut(s) 389, 590
BtsIMutI CAGTG 2 cut(s) 105, 288
CaiI CAGNNNCTG 2 cut(s) 290, 470
Cfr13I GGNCC 1 cut(s) 596
Csp6I GTAC 3 cut(s) 110, 330, 656
CviAII CATG 2 cut(s) 60, 669
CviQI GTAC 3 cut(s) 110, 330, 656
DdeI CTNAG 2 cut(s) 264, 522
DpnI GATC 4 cut(s) 126, 349, 468, 593
DpnII GATC 4 cut(s) 124, 347, 466, 591
Eco130I CCWWGG 1 cut(s) 343
EcoRII CCWGG 1 cut(s) 455
EcoT14I CCWWGG 1 cut(s) 343
ErhI CCWWGG 1 cut(s) 343
FaeI CATG 2 cut(s) 63, 672
FaiI YATR 7 cut(s) 53, 61, 93, 252, 398, 412, 670
FatI CATG 2 cut(s) 59, 668
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 2 cut(s) 402, 531
FokI GGATG 2 cut(s) 376, 597
Fsp4HI GCNGC 2 cut(s) 402, 531
GluI GCNGC 2 cut(s) 402, 531
HaeIII GGCC 1 cut(s) 597
HapII CCGG 1 cut(s) 581
Hin1II CATG 2 cut(s) 63, 672
HindIII AAGCTT 1 cut(s) 491
HinfI GANTC 2 cut(s) 218, 452
HpaII CCGG 1 cut(s) 581
HphI GGTGA 2 cut(s) 252, 601
Hpy188I TCNGA 2 cut(s) 466, 618
HpyAV CCTTC 3 cut(s) 13, 200, 319
HpyCH4III ACNGT 2 cut(s) 97, 109
HpyCH4V TGCA 4 cut(s) 82, 410, 545, 668
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 79, 407, 527
HpyF3I CTNAG 2 cut(s) 264, 522
Hsp92II CATG 2 cut(s) 63, 672
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 4 cut(s) 124, 347, 466, 591
LmnI GCTCC 2 cut(s) 38, 161
Lsp1109I GCAGC 2 cut(s) 413, 517
LweI GCATC 1 cut(s) 50
MaeIII GTNAC 2 cut(s) 31, 439
MalI GATC 4 cut(s) 126, 349, 468, 593
MboI GATC 4 cut(s) 124, 347, 466, 591
MboII GAAGA 1 cut(s) 370
MflI RGATCY 1 cut(s) 466
MluCI AATT 3 cut(s) 305, 309, 558
MnlI CCTC 7 cut(s) 5, 8, 80, 403, 517, 520, 609
MroXI GAANNNNTTC 2 cut(s) 174, 636
MseI TTAA 4 cut(s) 99, 308, 336, 642
MslI CAYNNNNRTG 1 cut(s) 667
MspI CCGG 1 cut(s) 581
MspR9I CCNGG 2 cut(s) 457, 582
MvaI CCWGG 1 cut(s) 457
MwoI GCNNNNNNNGC 4 cut(s) 70, 79, 407, 527
NciI CCSGG 1 cut(s) 582
NdeII GATC 4 cut(s) 124, 347, 466, 591
NlaIII CATG 2 cut(s) 63, 672
NlaIV GGNNCC 2 cut(s) 116, 633
NmuCI GTSAC 1 cut(s) 31
PdmI GAANNNNTTC 2 cut(s) 174, 636
PfeI GAWTC 2 cut(s) 218, 452
PfoI TCCNGGA 1 cut(s) 580
PkrI GCNGC 2 cut(s) 403, 532
PshBI ATTAAT 1 cut(s) 308
Psp6I CCWGG 1 cut(s) 455
PspGI CCWGG 1 cut(s) 455
PspN4I GGNNCC 2 cut(s) 116, 633
PspPI GGNCC 1 cut(s) 596
PstNI CAGNNNCTG 2 cut(s) 290, 470
PsuI RGATCY 1 cut(s) 466
RsaI GTAC 3 cut(s) 111, 331, 657
RsaNI GTAC 3 cut(s) 110, 330, 656
RseI CAYNNNNRTG 1 cut(s) 667
SaqAI TTAA 4 cut(s) 99, 308, 336, 642
SatI GCNGC 2 cut(s) 402, 531
Sau3AI GATC 4 cut(s) 124, 347, 466, 591
Sau96I GGNCC 1 cut(s) 596
ScrFI CCNGG 2 cut(s) 457, 582
SfaNI GCATC 1 cut(s) 50
SfcI CTRYAG 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 667
Sse9I AATT 3 cut(s) 305, 309, 558
SsiI CCGC 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 455, 580
StyI CCWWGG 1 cut(s) 343
TaaI ACNGT 2 cut(s) 97, 109
TasI AATT 3 cut(s) 305, 309, 558
TatI WGTACW 1 cut(s) 655
TfiI GAWTC 2 cut(s) 218, 452
Tru1I TTAA 4 cut(s) 99, 308, 336, 642
Tru9I TTAA 4 cut(s) 99, 308, 336, 642
TscAI CASTG 2 cut(s) 112, 295
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 2 cut(s) 401, 530
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 6 cut(s) 40, 48, 167, 219, 239, 465
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 2 cut(s) 112, 295
VspI ATTAAT 1 cut(s) 308
XmnI GAANNNNTTC 2 cut(s) 174, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.