RchiOBHm_Chr1g0317691

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000539
Physical Location & Seq
Reverse (-)
5264845 .. 5271477
6633 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54798

Sequence Viewer

Length: 1344 bp
ATGAGTACTAGAAGTATGGTAAAGTTTCTCTGGAAAAAGCGCATGCTATTTTTTTGTGAAGCTGCTGAGGCTTGTACAAACGACATGGCGTGTGGGAAATGTGGGCTTGGACCGAGCCAAGCTGGATGGGCTCTGAGTGGAACTCTTCCCTTTGAGGCTCTTGGGCTTAGTCACTTGGGCTGGGCTTCTTGCCTAGACAGGATTGACGGCCTTCTACATCGGATCTGGACAATGACGGTTGTGGTTGGAGACGGTGCCGTTTTCTGTTCGGCGATGATGGTTATTGACTATCCCGTGGATAGCGATGTCAGTCTCCACCGTCACAAGCATTCAGCTCAGTCACTCATTACCAGAGAGAACCAGAGGACGAGGATGAGCTGCGGAGAAAGCAAGGTTGTGTGTGTGACAGGAGCATCTGGGTTCATAGCATCATGGCTAGTCAAGCTCTTATTGCAACGAGGTTATACTGTCAAAGCCACTGTTCGGGACCCAAATGATCAAAAGAAAACAGAGCACCTACTCTCATTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCGAAGCAGACTTGTTAGAAGAAGGTTCTTTTGACCCTGTCGTTGATGGGTGTGAATGTGTTTTTCATACAGCATCCCCTGTCCTACTCTCATCTACTAATCCGCAGGCAGAATTAATTGAGCCTGCTTTGAAGGGAACGCTTAATGTCCTTGGATCGTGTGTGAAGGTCCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATGGCAGCAGTTAGATTTAATGGAAAACCTCTTACTGCTGATGTAATAATCGATGAATCTTGGTTTTCAGATCCTGCTTTTTGTGAAAAAGAAAAGCTTTGGTATATGCTTTCAAAGACGTTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAGAAAGGAATTGATATTATTACAATAAATCCGGGATGGGTGATCGGCCCTCCCTTACAGCCAACTCTGAACTTAACTGTGGAACTAGTTCTGAAACTCGTAAATGGGACCGAAAAGTTTCCCAACGACACCTACAGATTTGTTGATGTTAGAGATGTTGCTAATGCACATATTCTAGCCTTTGAAAACCCATCTGCTAGTGGACGTTATTGTTTAGTTGGAAAAGTAAAACACTGTTCAGAGGTTGTGAAAATGTTGCACGAGATCTTCCCTGCTCTCAATCTTCCAGATAAATGTGAAGATGACAAGCCTTTCACACCAACTCACCAGGTATCCAAGGAAAGAACCCAAACTTTGGGTGTAAAATATACTCCGCTTGAAGTGTCTCTGAAGGATACTGTTGAAAGTTTGAAGGACAAGAACTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

447

Amino Acids

49.53

Weight (kDa)

6.47

Isoelectric Point (pI)

33.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 130 - 216 2e-07 NmrA-like family
Epimerase PF01370 133 - 371 1.1e-23 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 135 - 367 2.7e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 135 - 324 5.4e-13 Male sterility protein
GDP_Man_Dehyd PF16363 135 - 379 2.4e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 137 - 258 4.2e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 749
AccB1I GGYRCC 1 cut(s) 254
AccB7I CCANNNNNTGG 1 cut(s) 1267
AciI CCGC 3 cut(s) 381, 659, 1286
AclWI GGATC 3 cut(s) 230, 718, 824
AcuI CTGAAG 1 cut(s) 1322
AfaI GTAC 2 cut(s) 7, 76
AfiI CCNNNNNNNGG 2 cut(s) 483, 1267
AgsI TTSAA 6 cut(s) 688, 873, 1097, 1292, 1316, 1324
AhlI ACTAGT 1 cut(s) 997
AjnI CCWGG 1 cut(s) 1239
AluBI AGCT 8 cut(s) 62, 122, 335, 378, 445, 856, 884, 893
AluI AGCT 8 cut(s) 62, 122, 335, 378, 445, 856, 884, 893
Alw21I GWGCWC 1 cut(s) 516
Alw26I GTCTC 3 cut(s) 243, 317, 1302
AlwI GGATC 3 cut(s) 230, 718, 824
AlwNI CAGNNNCTG 1 cut(s) 833
AoxI GGCC 2 cut(s) 208, 958
ApeKI GCWGC 4 cut(s) 62, 378, 764, 893
AseI ATTAAT 1 cut(s) 671
Asp700I GAANNNNTTC 4 cut(s) 546, 999, 1029, 1337
AspLEI GCGC 1 cut(s) 42
AspS9I GGNCC 5 cut(s) 110, 487, 724, 959, 1020
AsuC2I CCSGG 1 cut(s) 945
AsuHPI GGTGA 2 cut(s) 964, 1229
AsuII TTCGAA 1 cut(s) 558
AvaII GGWCC 4 cut(s) 110, 487, 724, 1020
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 1 cut(s) 133
BauI CACGAG 1 cut(s) 1172
Bbv12I GWGCWC 1 cut(s) 516
BbvCI CCTCAGC 2 cut(s) 66, 885
BbvI GCAGC 4 cut(s) 49, 365, 776, 880
BccI CCATC 6 cut(s) 120, 271, 524, 596, 942, 1111
BceAI ACGGC 2 cut(s) 223, 242
BciT130I CCWGG 1 cut(s) 1241
BciVI GTATCC 2 cut(s) 1255, 1300
BclI TGATCA 1 cut(s) 496
BcnI CCSGG 1 cut(s) 945
BcoDI GTCTC 3 cut(s) 243, 317, 1302
BcuI ACTAGT 1 cut(s) 997
BfaI CTAG 6 cut(s) 9, 194, 437, 998, 1088, 1110
BfmI CTRYAG 1 cut(s) 1045
BfuI GTATCC 2 cut(s) 1255, 1300
BglII AGATCT 1 cut(s) 1176
BisI GCNGC 4 cut(s) 63, 379, 765, 894
BlsI GCNGC 4 cut(s) 64, 380, 766, 895
BmcAI AGTACT 1 cut(s) 7
Bme1390I CCNGG 2 cut(s) 945, 1241
Bme18I GGWCC 4 cut(s) 110, 487, 724, 1020
BmgT120I GGNCC 5 cut(s) 110, 487, 724, 959, 1020
BmiI GGNNCC 4 cut(s) 256, 488, 489, 1021
BmrFI CCNGG 2 cut(s) 945, 1241
BmsI GCATC 3 cut(s) 422, 437, 638
BplI GAGNNNNNCTC 2 cut(s) 127, 159
Bpu10I CCTNAGC 2 cut(s) 66, 885
Bpu14I TTCGAA 1 cut(s) 558
BpuMI CCSGG 1 cut(s) 945
Bsa29I ATCGAT 1 cut(s) 810
BsaJI CCNNGG 3 cut(s) 294, 706, 1248
Bsc4I CCNNNNNNNGG 2 cut(s) 483, 1267
Bse1I ACTGG 1 cut(s) 727
BseBI CCWGG 1 cut(s) 1241
BseCI ATCGAT 1 cut(s) 810
BseDI CCNNGG 3 cut(s) 294, 706, 1248
BseGI GGATG 5 cut(s) 131, 378, 629, 752, 953
BseLI CCNNNNNNNGG 2 cut(s) 483, 1267
BseMII CTCAG 4 cut(s) 57, 125, 350, 876
BseNI ACTGG 1 cut(s) 727
BseXI GCAGC 4 cut(s) 49, 365, 776, 880
BseYI CCCAGC 1 cut(s) 180
BshFI GGCC 2 cut(s) 210, 960
BshNI GGYRCC 1 cut(s) 254
BshVI ATCGAT 1 cut(s) 810
BsiHKAI GWGCWC 1 cut(s) 516
BsiSI CCGG 1 cut(s) 944
BslFI GGGAC 2 cut(s) 500, 1033
BslI CCNNNNNNNGG 2 cut(s) 483, 1267
BsmAI GTCTC 3 cut(s) 243, 317, 1302
BsmBI CGTCTC 1 cut(s) 243
BsmFI GGGAC 2 cut(s) 500, 1033
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 2 cut(s) 210, 960
Bsp119I TTCGAA 1 cut(s) 558
Bsp1286I GDGCHC 2 cut(s) 133, 516
Bsp1407I TGTACA 1 cut(s) 74
Bsp143I GATC 6 cut(s) 222, 496, 710, 829, 954, 1176
BspACI CCGC 3 cut(s) 381, 659, 1286
BspANI GGCC 2 cut(s) 210, 960
BspCNI CTCAG 4 cut(s) 58, 126, 349, 877
BspDI ATCGAT 1 cut(s) 810
BspLI GGNNCC 4 cut(s) 256, 488, 489, 1021
BspPI GGATC 3 cut(s) 230, 718, 824
BspT104I TTCGAA 1 cut(s) 558
BspT107I GGYRCC 1 cut(s) 254
BsrGI TGTACA 1 cut(s) 74
BsrI ACTGG 1 cut(s) 727
BssECI CCNNGG 3 cut(s) 294, 706, 1248
BssMI GATC 6 cut(s) 222, 496, 710, 829, 954, 1176
BssSI CACGAG 1 cut(s) 1172
BssT1I CCWWGG 2 cut(s) 706, 1248
Bst2BI CACGAG 1 cut(s) 1172
Bst2UI CCWGG 1 cut(s) 1241
Bst4CI ACNGT 8 cut(s) 238, 254, 320, 469, 481, 991, 1148, 1312
Bst6I CTCTTC 1 cut(s) 150
BstAUI TGTACA 1 cut(s) 74
BstBI TTCGAA 1 cut(s) 558
BstC8I GCNNGC 3 cut(s) 44, 663, 681
BstDEI CTNAG 5 cut(s) 66, 134, 167, 336, 885
BstDSI CCRYGG 1 cut(s) 294
BstF5I GGATG 5 cut(s) 131, 378, 629, 752, 953
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 6 cut(s) 225, 499, 713, 832, 957, 1179
BstMAI GTCTC 3 cut(s) 243, 317, 1302
BstMBI GATC 6 cut(s) 222, 496, 710, 829, 954, 1176
BstMWI GCNNNNNNNGC 6 cut(s) 68, 128, 387, 442, 451, 890
BstNI CCWGG 1 cut(s) 1241
BstNSI RCATGY 1 cut(s) 46
BstSCI CCNGG 2 cut(s) 943, 1239
BstSFI CTRYAG 1 cut(s) 1045
BstV1I GCAGC 4 cut(s) 49, 365, 776, 880
BstX2I RGATCY 3 cut(s) 222, 829, 1176
BstYI RGATCY 3 cut(s) 222, 829, 1176
Bsu15I ATCGAT 1 cut(s) 810
BsuI GTATCC 2 cut(s) 1255, 1300
BsuRI GGCC 2 cut(s) 210, 960
BsuTUI ATCGAT 1 cut(s) 810
BtgI CCRYGG 1 cut(s) 294
BtgZI GCGATG 2 cut(s) 287, 318
BtsCI GGATG 5 cut(s) 131, 378, 629, 752, 953
BtsIMutI CAGTG 2 cut(s) 477, 1144
Cac8I GCNNGC 3 cut(s) 44, 663, 681
CaiI CAGNNNCTG 1 cut(s) 833
CfoI GCGC 1 cut(s) 42
Cfr13I GGNCC 5 cut(s) 110, 487, 724, 959, 1020
ClaI ATCGAT 1 cut(s) 810
CsiI ACCWGGT 1 cut(s) 1239
Csp6I GTAC 2 cut(s) 6, 75
CviAII CATG 3 cut(s) 43, 85, 432
CviQI GTAC 2 cut(s) 6, 75
DdeI CTNAG 5 cut(s) 66, 134, 167, 336, 885
DpnI GATC 6 cut(s) 224, 498, 712, 831, 956, 1178
DpnII GATC 6 cut(s) 222, 496, 710, 829, 954, 1176
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco130I CCWWGG 2 cut(s) 706, 1248
Eco24I GRGCYC 1 cut(s) 133
Eco47I GGWCC 4 cut(s) 110, 487, 724, 1020
Eco57I CTGAAG 1 cut(s) 1322
EcoO109I RGGNCCY 1 cut(s) 487
EcoRII CCWGG 1 cut(s) 1239
EcoT14I CCWWGG 2 cut(s) 706, 1248
EcoT38I GRGCYC 1 cut(s) 133
ErhI CCWWGG 2 cut(s) 706, 1248
Esp3I CGTCTC 1 cut(s) 243
FaeI CATG 3 cut(s) 46, 88, 435
FalI AAGNNNNNCTT 3 cut(s) 840, 872, 1322
FaqI GGGAC 2 cut(s) 500, 1033
FatI CATG 3 cut(s) 42, 84, 431
FbaI TGATCA 1 cut(s) 496
Fnu4HI GCNGC 4 cut(s) 63, 379, 765, 894
FokI GGATG 5 cut(s) 138, 385, 616, 739, 960
FriOI GRGCYC 1 cut(s) 133
Fsp4HI GCNGC 4 cut(s) 63, 379, 765, 894
FspBI CTAG 6 cut(s) 9, 194, 437, 998, 1088, 1110
GlaI GCGC 1 cut(s) 41
GluI GCNGC 4 cut(s) 63, 379, 765, 894
GsaI CCCAGC 1 cut(s) 184
HaeIII GGCC 2 cut(s) 210, 960
HapII CCGG 1 cut(s) 944
HhaI GCGC 1 cut(s) 42
Hin1II CATG 3 cut(s) 46, 88, 435
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 854
HinfI GANTC 1 cut(s) 815
HpaII CCGG 1 cut(s) 944
HphI GGTGA 2 cut(s) 964, 1229
Hpy166II GTNNAC 1 cut(s) 1115
Hpy188I TCNGA 7 cut(s) 135, 222, 829, 981, 1005, 1153, 1302
Hpy188III TCNNGA 4 cut(s) 31, 226, 485, 1199
Hpy8I GTNNAC 1 cut(s) 1115
HpyAV CCTTC 6 cut(s) 221, 572, 682, 715, 1297, 1318
HpyCH4III ACNGT 8 cut(s) 238, 254, 320, 469, 481, 991, 1148, 1312
HpyCH4IV ACGT 2 cut(s) 878, 1117
HpyCH4V TGCA 4 cut(s) 454, 908, 1079, 1171
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 128, 387, 442, 451, 890
HpyF3I CTNAG 5 cut(s) 66, 134, 167, 336, 885
HpySE526I ACGT 2 cut(s) 878, 1117
Hsp92II CATG 3 cut(s) 46, 88, 435
HspAI GCGC 1 cut(s) 40
KflI GGGWCCC 1 cut(s) 487
Ksp22I TGATCA 1 cut(s) 496
Kzo9I GATC 6 cut(s) 222, 496, 710, 829, 954, 1176
LmnI GCTCC 2 cut(s) 410, 533
Lsp1109I GCAGC 4 cut(s) 49, 365, 776, 880
LweI GCATC 3 cut(s) 422, 437, 638
MabI ACCWGGT 1 cut(s) 1239
MaeI CTAG 6 cut(s) 9, 194, 437, 998, 1088, 1110
MaeII ACGT 2 cut(s) 878, 1117
MaeIII GTNAC 4 cut(s) 170, 320, 339, 403
MalI GATC 6 cut(s) 224, 498, 712, 831, 956, 1178
MboI GATC 6 cut(s) 222, 496, 710, 829, 954, 1176
MboII GAAGA 6 cut(s) 137, 587, 1171, 1187, 1223, 1330
MflI RGATCY 3 cut(s) 222, 829, 1176
MhlI GDGCHC 2 cut(s) 133, 516
MluCI AATT 3 cut(s) 668, 672, 921
MmeI TCCRAC 2 cut(s) 226, 1111
MroXI GAANNNNTTC 4 cut(s) 546, 999, 1029, 1337
MseI TTAA 4 cut(s) 671, 699, 777, 986
MspI CCGG 1 cut(s) 944
MspR9I CCNGG 2 cut(s) 945, 1241
Mva1269I GAATGC 1 cut(s) 328
MvaI CCWGG 1 cut(s) 1241
MwoI GCNNNNNNNGC 6 cut(s) 68, 128, 387, 442, 451, 890
NciI CCSGG 1 cut(s) 945
NdeII GATC 6 cut(s) 222, 496, 710, 829, 954, 1176
NlaIII CATG 3 cut(s) 46, 88, 435
NlaIV GGNNCC 4 cut(s) 256, 488, 489, 1021
NmuCI GTSAC 4 cut(s) 170, 320, 339, 403
NspI RCATGY 1 cut(s) 46
NspV TTCGAA 1 cut(s) 558
PaeI GCATGC 1 cut(s) 46
PctI GAATGC 1 cut(s) 328
PdmI GAANNNNTTC 4 cut(s) 546, 999, 1029, 1337
PfeI GAWTC 1 cut(s) 815
PflFI GACNNNGTC 1 cut(s) 593
PflMI CCANNNNNTGG 1 cut(s) 1267
PfoI TCCNGGA 1 cut(s) 943
PkrI GCNGC 4 cut(s) 64, 380, 766, 895
PpuMI RGGWCCY 1 cut(s) 487
PshBI ATTAAT 1 cut(s) 671
PsiI TTATAA 1 cut(s) 749
Psp5II RGGWCCY 1 cut(s) 487
Psp6I CCWGG 1 cut(s) 1239
PspFI CCCAGC 1 cut(s) 180
PspGI CCWGG 1 cut(s) 1239
PspN4I GGNNCC 4 cut(s) 256, 488, 489, 1021
PspPI GGNCC 5 cut(s) 110, 487, 724, 959, 1020
PspPPI RGGWCCY 1 cut(s) 487
PstNI CAGNNNCTG 1 cut(s) 833
PsuI RGATCY 3 cut(s) 222, 829, 1176
PsyI GACNNNGTC 1 cut(s) 593
RsaI GTAC 2 cut(s) 7, 76
RsaNI GTAC 2 cut(s) 6, 75
SaqAI TTAA 4 cut(s) 671, 699, 777, 986
SatI GCNGC 4 cut(s) 63, 379, 765, 894
Sau3AI GATC 6 cut(s) 222, 496, 710, 829, 954, 1176
Sau96I GGNCC 5 cut(s) 110, 487, 724, 959, 1020
ScaI AGTACT 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 945, 1241
SduI GDGCHC 2 cut(s) 133, 516
SexAI ACCWGGT 1 cut(s) 1239
SfaNI GCATC 3 cut(s) 422, 437, 638
SfcI CTRYAG 1 cut(s) 1045
SfuI TTCGAA 1 cut(s) 558
SinI GGWCC 4 cut(s) 110, 487, 724, 1020
SpeI ACTAGT 1 cut(s) 997
SphI GCATGC 1 cut(s) 46
Sse9I AATT 3 cut(s) 668, 672, 921
SsiI CCGC 3 cut(s) 381, 659, 1286
SspMI CTAG 6 cut(s) 9, 194, 437, 998, 1088, 1110
StyD4I CCNGG 2 cut(s) 943, 1239
StyI CCWWGG 2 cut(s) 706, 1248
TaaI ACNGT 8 cut(s) 238, 254, 320, 469, 481, 991, 1148, 1312
TaiI ACGT 2 cut(s) 881, 1120
TaqI TCGA 2 cut(s) 558, 810
TaqII GACCGA 2 cut(s) 127, 1037
TasI AATT 3 cut(s) 668, 672, 921
TatI WGTACW 2 cut(s) 5, 74
TfiI GAWTC 1 cut(s) 815
Tru1I TTAA 4 cut(s) 671, 699, 777, 986
Tru9I TTAA 4 cut(s) 671, 699, 777, 986
TscAI CASTG 2 cut(s) 484, 1151
TseFI GTSAC 4 cut(s) 170, 320, 339, 403
TseI GCWGC 4 cut(s) 62, 378, 764, 893
Tsp45I GTSAC 4 cut(s) 170, 320, 339, 403
TspDTI ATGAA 4 cut(s) 412, 539, 611, 828
TspRI CASTG 2 cut(s) 484, 1151
Tth111I GACNNNGTC 1 cut(s) 593
Van91I CCANNNNNTGG 1 cut(s) 1267
VpaK11BI GGWCC 4 cut(s) 110, 487, 724, 1020
VspI ATTAAT 1 cut(s) 671
XceI RCATGY 1 cut(s) 46
XmnI GAANNNNTTC 4 cut(s) 546, 999, 1029, 1337
XspI CTAG 6 cut(s) 9, 194, 437, 998, 1088, 1110
ZrmI AGTACT 1 cut(s) 7
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.