MD11G1190800.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000539
Physical Location & Seq
Forward (+)
27003483 .. 27009027
5545 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1190800.v1.1.491

Sequence Viewer

Length: 978 bp
ATGAGCAGCGGAGCAGGGAAGGTCGTGTGCGTCACCGGTGCGTCTGGATACATTGCTTCATGGCTCGTTAAGCTTCTTCTCCAGCGCGGCTACACCGTCAAGGCCTCTATTCGCGACCCAAATGATCCAACTAAGACAGAGCACCTGCATGCGCTTGATGGAGCCCAAGACAGACTTCAACTTTTCAAAGCAAATCTGTTGGAAGAAGGTTCTTTTGACTCTGCTGTTGAGGGTTGTGAAGGCGTATTCCATACTGCATCTCCCTTTTATCATGATGTCACAGACCCAAAGGCAGAACTACTTGAACCTGCGGTAAAGGGGACCCTTAATGTTCTTAATTCGTGTGCGAAATCACCATCAATCAAACGGGTGGTTTTGACATCTTCTATAGCCGCTGTAGCATATAATGGAAAGCCTCGAACTCCTGATGTAGTAGTTGATGAGACTTGGTTTACAGATCCAGATGTTTGTAAGGAATCAAAGCTTTGGTACGTGCTTTCAAAGACTTTGGCTGAAGATGCTGCGTGGAAATTTGTAAAGGAGAAGGGTATTGACTTGGTTACAATTAACCCCGCAATGGTGATAGGTCCTCTGTTACAGCCAACTCTTAATACAAGTGCTGCAGCAGTTTTAAATGTTATTAAGGGAGCTCGAACTTTTCCAAATGCAAGTTTTGGATGGATTAACGTTAAAGATGTTGCCAATGCACATATTCAAGCATTTGAGAGGCCTACCGCTAGTGGCAGATATTGTTTGGTTGAGAGAGTTGCCCACTTCTCAGAAGTTGTGAGAATTTTACGTGAGCTGTACCCTACTTTGCAACTTCCAGAGAAGTGTGCAGACGACAAGCCTTTTGTGCCAACATATCAGGTGTCCAAGGAGAAGGCGAAGAGCTTGGGTGTCGAATTTATTCCACTAGATGTTAGCCTCAAGGAAACAGTTGAAAGTCTGAAGGAAAAGGGTTTTGTCAATTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

326

Amino Acids

35.55

Weight (kDa)

6.61

Isoelectric Point (pI)

26.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 92 4.8e-09 NmrA-like family
Epimerase PF01370 9 - 246 1.4e-22 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 241 3e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 11 - 222 5.9e-12 Male sterility protein
GDP_Man_Dehyd PF16363 11 - 256 2.3e-10 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 147 6.6e-11 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 153
Acc36I ACCTGC 2 cut(s) 153, 316
AccII CGCG 2 cut(s) 87, 114
AciI CCGC 6 cut(s) 9, 87, 311, 393, 573, 735
AclI AACGTT 1 cut(s) 687
AclWI GGATC 2 cut(s) 119, 452
AcsI RAATTY 3 cut(s) 530, 792, 905
AcuI CTGAAG 2 cut(s) 534, 971
AfaI GTAC 2 cut(s) 491, 809
AfiI CCNNNNNNNGG 1 cut(s) 577
AgeI ACCGGT 1 cut(s) 35
AgsI TTSAA 6 cut(s) 179, 187, 305, 501, 716, 944
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AluBI AGCT 5 cut(s) 73, 484, 650, 805, 894
AluI AGCT 5 cut(s) 73, 484, 650, 805, 894
Alw21I GWGCWC 2 cut(s) 144, 652
Alw26I GTCTC 1 cut(s) 437
AlwI GGATC 2 cut(s) 119, 452
AoxI GGCC 2 cut(s) 102, 728
ApeKI GCWGC 4 cut(s) 6, 521, 620, 623
ApoI RAATTY 3 cut(s) 530, 792, 905
ArsI GACNNNNNNTTYG 2 cut(s) 836, 868
AsiGI ACCGGT 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 909
AspLEI GCGC 2 cut(s) 87, 154
AspS9I GGNCC 2 cut(s) 321, 587
AsuHPI GGTGA 3 cut(s) 25, 345, 592
AvaII GGWCC 2 cut(s) 321, 587
BanII GRGCYC 2 cut(s) 166, 652
Bbv12I GWGCWC 2 cut(s) 144, 652
BbvI GCAGC 4 cut(s) 18, 508, 607, 635
BccI CCATC 3 cut(s) 152, 364, 672
BciVI GTATCC 1 cut(s) 41
BcoDI GTCTC 1 cut(s) 437
BfaI CTAG 2 cut(s) 738, 917
BfmI CTRYAG 3 cut(s) 387, 396, 621
BfuAI ACCTGC 2 cut(s) 153, 316
BfuI GTATCC 1 cut(s) 41
BisI GCNGC 6 cut(s) 7, 88, 393, 522, 621, 624
BlsI GCNGC 6 cut(s) 8, 89, 394, 523, 622, 625
Bme18I GGWCC 2 cut(s) 321, 587
BmgT120I GGNCC 2 cut(s) 321, 587
BmiI GGNNCC 3 cut(s) 163, 322, 323
BmsI GCATC 2 cut(s) 266, 508
BpmI CTGGAG 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 914
BsaAI YACGTR 2 cut(s) 493, 800
BsaJI CCNNGG 1 cut(s) 876
BsaWI WCCGGW 1 cut(s) 35
BsaXI ACNNNNNCTCC 2 cut(s) 244, 274
Bsc4I CCNNNNNNNGG 1 cut(s) 577
Bse118I RCCGGY 1 cut(s) 35
Bse3DI GCAATG 2 cut(s) 51, 582
BseDI CCNNGG 1 cut(s) 876
BseGI GGATG 1 cut(s) 683
BseLI CCNNNNNNNGG 1 cut(s) 577
BseMI GCAATG 2 cut(s) 51, 582
BseMII CTCAG 1 cut(s) 792
BseXI GCAGC 4 cut(s) 18, 508, 607, 635
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 2 cut(s) 87, 114
BshFI GGCC 2 cut(s) 104, 730
BshTI ACCGGT 1 cut(s) 35
BsiHKAI GWGCWC 2 cut(s) 144, 652
BsiSI CCGG 1 cut(s) 36
BslFI GGGAC 1 cut(s) 334
BslI CCNNNNNNNGG 1 cut(s) 577
BsmAI GTCTC 1 cut(s) 437
BsmFI GGGAC 1 cut(s) 334
BsnI GGCC 2 cut(s) 104, 730
Bsp1286I GDGCHC 3 cut(s) 144, 166, 652
Bsp143I GATC 2 cut(s) 124, 457
Bsp68I TCGCGA 1 cut(s) 114
BspACI CCGC 6 cut(s) 9, 87, 311, 393, 573, 735
BspANI GGCC 2 cut(s) 104, 730
BspCNI CTCAG 1 cut(s) 791
BspFNI CGCG 2 cut(s) 87, 114
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 3 cut(s) 163, 322, 323
BspMAI CTGCAG 1 cut(s) 625
BspMI ACCTGC 2 cut(s) 153, 316
BspPI GGATC 2 cut(s) 119, 452
BspQI GCTCTTC 1 cut(s) 884
BsrDI GCAATG 2 cut(s) 51, 582
BsrFI RCCGGY 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 1 cut(s) 876
BssMI GATC 2 cut(s) 124, 457
BssT1I CCWWGG 1 cut(s) 876
Bst4CI ACNGT 2 cut(s) 97, 940
Bst6I CTCTTC 1 cut(s) 884
BstBAI YACGTR 2 cut(s) 493, 800
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 2 cut(s) 132, 778
BstF5I GGATG 1 cut(s) 683
BstFNI CGCG 2 cut(s) 87, 114
BstHHI GCGC 2 cut(s) 87, 154
BstKTI GATC 2 cut(s) 127, 460
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 2 cut(s) 124, 457
BstMWI GCNNNNNNNGC 4 cut(s) 70, 398, 518, 856
BstNSI RCATGY 1 cut(s) 152
BstSFI CTRYAG 3 cut(s) 387, 396, 621
BstUI CGCG 2 cut(s) 87, 114
BstV1I GCAGC 4 cut(s) 18, 508, 607, 635
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
BsuI GTATCC 1 cut(s) 41
BsuRI GGCC 2 cut(s) 104, 730
BtsCI GGATG 1 cut(s) 683
BtuMI TCGCGA 1 cut(s) 114
BveI ACCTGC 2 cut(s) 153, 316
Cac8I GCNNGC 1 cut(s) 150
CciI TCATGA 1 cut(s) 271
CfoI GCGC 2 cut(s) 87, 154
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 321, 587
CseI GACGC 2 cut(s) 19, 30
Csp6I GTAC 2 cut(s) 490, 808
CspAI ACCGGT 1 cut(s) 35
CviAII CATG 3 cut(s) 60, 149, 272
CviQI GTAC 2 cut(s) 490, 808
DdeI CTNAG 2 cut(s) 132, 778
DpnI GATC 2 cut(s) 126, 459
DpnII GATC 2 cut(s) 124, 457
DraI TTTAAA 1 cut(s) 633
Eam1104I CTCTTC 1 cut(s) 884
EarI CTCTTC 1 cut(s) 884
Ecl136II GAGCTC 1 cut(s) 650
Eco130I CCWWGG 1 cut(s) 876
Eco147I AGGCCT 2 cut(s) 104, 730
Eco24I GRGCYC 2 cut(s) 166, 652
Eco47I GGWCC 2 cut(s) 321, 587
Eco53kI GAGCTC 1 cut(s) 650
Eco57I CTGAAG 2 cut(s) 534, 971
EcoICRI GAGCTC 1 cut(s) 650
EcoO109I RGGNCCY 2 cut(s) 321, 587
EcoT14I CCWWGG 1 cut(s) 876
EcoT38I GRGCYC 2 cut(s) 166, 652
ErhI CCWWGG 1 cut(s) 876
FaeI CATG 3 cut(s) 63, 152, 275
FaiI YATR 9 cut(s) 61, 150, 252, 273, 389, 403, 405, 711, 865
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FaqI GGGAC 1 cut(s) 334
FatI CATG 3 cut(s) 59, 148, 271
FauI CCCGC 1 cut(s) 580
Fnu4HI GCNGC 6 cut(s) 7, 88, 393, 522, 621, 624
FokI GGATG 1 cut(s) 690
FriOI GRGCYC 2 cut(s) 166, 652
Fsp4HI GCNGC 6 cut(s) 7, 88, 393, 522, 621, 624
FspBI CTAG 2 cut(s) 738, 917
GlaI GCGC 2 cut(s) 86, 153
GluI GCNGC 6 cut(s) 7, 88, 393, 522, 621, 624
GsuI CTGGAG 1 cut(s) 65
HaeIII GGCC 2 cut(s) 104, 730
HapII CCGG 1 cut(s) 36
HgaI GACGC 2 cut(s) 19, 30
HhaI GCGC 2 cut(s) 87, 154
Hin1II CATG 3 cut(s) 63, 152, 275
Hin6I GCGC 2 cut(s) 85, 152
HinP1I GCGC 2 cut(s) 85, 152
HindIII AAGCTT 2 cut(s) 71, 482
HinfI GANTC 2 cut(s) 218, 476
HpaII CCGG 1 cut(s) 36
HphI GGTGA 3 cut(s) 25, 345, 592
Hpy166II GTNNAC 1 cut(s) 453
Hpy188I TCNGA 3 cut(s) 781, 951, 977
Hpy188III TCNNGA 6 cut(s) 45, 113, 272, 425, 461, 827
Hpy8I GTNNAC 1 cut(s) 453
HpyAV CCTTC 6 cut(s) 13, 200, 233, 538, 877, 946
HpyCH4III ACNGT 2 cut(s) 97, 940
HpyCH4IV ACGT 3 cut(s) 492, 687, 799
HpyCH4V TGCA 7 cut(s) 148, 257, 623, 668, 707, 820, 839
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 398, 518, 856
HpyF3I CTNAG 2 cut(s) 132, 778
HpySE526I ACGT 3 cut(s) 492, 687, 799
Hsp92II CATG 3 cut(s) 63, 152, 275
HspAI GCGC 2 cut(s) 85, 152
KflI GGGWCCC 1 cut(s) 321
Kzo9I GATC 2 cut(s) 124, 457
LguI GCTCTTC 1 cut(s) 884
LmnI GCTCC 3 cut(s) 11, 161, 647
LpnPI CCDG 9 cut(s) 30, 49, 95, 158, 321, 438, 474, 840, 854
Lsp1109I GCAGC 4 cut(s) 18, 508, 607, 635
LweI GCATC 2 cut(s) 266, 508
MaeI CTAG 2 cut(s) 738, 917
MaeII ACGT 3 cut(s) 492, 687, 799
MaeIII GTNAC 4 cut(s) 31, 277, 559, 594
MalI GATC 2 cut(s) 126, 459
MboI GATC 2 cut(s) 124, 457
MboII GAAGA 5 cut(s) 68, 215, 375, 527, 901
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 3 cut(s) 144, 166, 652
MluCI AATT 6 cut(s) 337, 530, 564, 792, 905, 970
MlyI GAGTC 1 cut(s) 212
MmeI TCCRAC 2 cut(s) 152, 180
MnlI CCTC 6 cut(s) 115, 223, 426, 600, 720, 938
MroXI GAANNNNTTC 1 cut(s) 909
MseI TTAA 9 cut(s) 69, 327, 336, 567, 609, 632, 642, 684, 690
MslI CAYNNNNRTG 1 cut(s) 147
MspA1I CMGCKG 2 cut(s) 9, 395
MspI CCGG 1 cut(s) 36
MvnI CGCG 2 cut(s) 87, 114
MwoI GCNNNNNNNGC 4 cut(s) 70, 398, 518, 856
NdeII GATC 2 cut(s) 124, 457
NlaIII CATG 3 cut(s) 63, 152, 275
NlaIV GGNNCC 3 cut(s) 163, 322, 323
NmuCI GTSAC 2 cut(s) 31, 277
NruI TCGCGA 1 cut(s) 114
NspI RCATGY 1 cut(s) 152
PaeI GCATGC 1 cut(s) 152
PagI TCATGA 1 cut(s) 271
PaqCI CACCTGC 1 cut(s) 153
PceI AGGCCT 2 cut(s) 104, 730
PciSI GCTCTTC 1 cut(s) 884
PdmI GAANNNNTTC 1 cut(s) 909
PfeI GAWTC 1 cut(s) 476
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 6 cut(s) 8, 89, 394, 523, 622, 625
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Ppu21I YACGTR 2 cut(s) 493, 800
PpuMI RGGWCCY 2 cut(s) 321, 587
Psp124BI GAGCTC 1 cut(s) 652
Psp1406I AACGTT 1 cut(s) 687
Psp5II RGGWCCY 2 cut(s) 321, 587
PspN4I GGNNCC 3 cut(s) 163, 322, 323
PspPI GGNCC 2 cut(s) 321, 587
PspPPI RGGWCCY 2 cut(s) 321, 587
PstI CTGCAG 1 cut(s) 625
PsuI RGATCY 1 cut(s) 457
RruI TCGCGA 1 cut(s) 114
RsaI GTAC 2 cut(s) 491, 809
RsaNI GTAC 2 cut(s) 490, 808
RseI CAYNNNNRTG 1 cut(s) 147
SacI GAGCTC 1 cut(s) 652
SapI GCTCTTC 1 cut(s) 884
SaqAI TTAA 9 cut(s) 69, 327, 336, 567, 609, 632, 642, 684, 690
SatI GCNGC 6 cut(s) 7, 88, 393, 522, 621, 624
Sau3AI GATC 2 cut(s) 124, 457
Sau96I GGNCC 2 cut(s) 321, 587
SchI GAGTC 1 cut(s) 212
SduI GDGCHC 3 cut(s) 144, 166, 652
SfaNI GCATC 2 cut(s) 266, 508
SfcI CTRYAG 3 cut(s) 387, 396, 621
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 2 cut(s) 321, 587
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 1 cut(s) 929
SmoI CTYRAG 1 cut(s) 929
SphI GCATGC 1 cut(s) 152
Sse9I AATT 6 cut(s) 337, 530, 564, 792, 905, 970
SseBI AGGCCT 2 cut(s) 104, 730
SsiI CCGC 6 cut(s) 9, 87, 311, 393, 573, 735
SspMI CTAG 2 cut(s) 738, 917
SstI GAGCTC 1 cut(s) 652
StuI AGGCCT 2 cut(s) 104, 730
StyI CCWWGG 1 cut(s) 876
TaaI ACNGT 2 cut(s) 97, 940
TaiI ACGT 3 cut(s) 495, 690, 802
TaqI TCGA 3 cut(s) 418, 652, 903
TasI AATT 6 cut(s) 337, 530, 564, 792, 905, 970
TauI GCSGC 2 cut(s) 90, 395
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 9 cut(s) 69, 327, 336, 567, 609, 632, 642, 684, 690
Tru9I TTAA 9 cut(s) 69, 327, 336, 567, 609, 632, 642, 684, 690
TseFI GTSAC 2 cut(s) 31, 277
TseI GCWGC 4 cut(s) 6, 521, 620, 623
Tsp45I GTSAC 2 cut(s) 31, 277
TspDTI ATGAA 1 cut(s) 48
VpaK11BI GGWCC 2 cut(s) 321, 587
XapI RAATTY 3 cut(s) 530, 792, 905
XceI RCATGY 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 909
XspI CTAG 2 cut(s) 738, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.