RLG00000030605

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000539
Physical Location & Seq
Reverse (-)
64346612 .. 64349120
2509 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030605

Sequence Viewer

Length: 912 bp
ATGAGCTGCGGAGAAAGCAAGGTTGTGTGTGTGACGGGAGCATCTGGTTTCATAGCATCATGGCTGGTGAAGCTCTTATTGCAACGAGGTTATACTGTCAAAGCCACGGTTCGGGACCCAAACGATCAAAAGAAAACAGAACACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGGTCTTTTTATACGGCAATTGATGGATGTGAATGTGTTTTCCATACGGCGTCCCCTGTCCTACGCTCAGTCTCATCAACTAATCCGCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATGGCAGCAGTTGGATTTAATGGAAAACCTCTTGCTGCTGATGTAATAATCGACGAATCTTGGTTTTCAGATCCTGCTTTTTGTGAAAAAACAAAGCTTTGGTATATGCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAAAAAGGAATTGATATTATTACAATAAATCCGGGATGGGTGATCGGCCCTCTCTTACAGCCAACTCTGAACTTGAGTGCGGAACTAGTTCTGAAACTCGTAAATGGGACCGAAAAGTTTCCCAACAGAACTTACAGACTTGTTGATGTTAGAGATGTTGCTATTGCACATATTCTAGCCTTTGAAAACCCATCAGCTAGTGGACGTTATTGTTTAGTTGGAAGCGTAAAACACTGTTCAGAGGTTGTGAAAATGTTGTACGAGATCTCCCCTGCTCTCAATCTTCCAGATAAATGTGCAGATGACAAGCCTTTCACACCAACCAACCAGGTATCCAAGGAAAGAACCCAAACTTTGGGTGTAAAGTATACTCCGCTAGAAGTGTCTCTGAAGGATACTGTTGAAAGTTTGAAGAACAAGAACTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

304

Amino Acids

33.45

Weight (kDa)

8.03

Isoelectric Point (pI)

29.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 87 1.9e-08 NmrA-like family
Epimerase PF01370 9 - 227 1.6e-15 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 103 5.2e-07 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 105 1.1e-09 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 112 9.8e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 317
AccB7I CCANNNNNTGG 1 cut(s) 835
AccI GTMKAC 1 cut(s) 848
AciI CCGC 4 cut(s) 9, 293, 560, 854
AclWI GGATC 1 cut(s) 392
AcuI CTGAAG 1 cut(s) 890
AcyI GRCGYC 1 cut(s) 257
AfaI GTAC 1 cut(s) 740
AfiI CCNNNNNNNGG 2 cut(s) 111, 835
AgsI TTSAA 5 cut(s) 187, 441, 665, 884, 892
AhlI ACTAGT 1 cut(s) 565
AjnI CCWGG 1 cut(s) 807
AluBI AGCT 6 cut(s) 6, 73, 424, 452, 461, 677
AluI AGCT 6 cut(s) 6, 73, 424, 452, 461, 677
Alw26I GTCTC 2 cut(s) 283, 870
AlwI GGATC 1 cut(s) 392
AlwNI CAGNNNCTG 1 cut(s) 401
AoxI GGCC 1 cut(s) 526
ApeKI GCWGC 4 cut(s) 6, 332, 362, 461
Asp700I GAANNNNTTC 4 cut(s) 174, 567, 597, 905
AspS9I GGNCC 3 cut(s) 115, 527, 588
AsuC2I CCSGG 1 cut(s) 513
AsuHPI GGTGA 2 cut(s) 79, 532
AvaII GGWCC 2 cut(s) 115, 588
BbvCI CCTCAGC 1 cut(s) 453
BbvI GCAGC 3 cut(s) 344, 349, 448
BccI CCATC 4 cut(s) 152, 224, 510, 679
BceAI ACGGC 2 cut(s) 237, 270
BciT130I CCWGG 1 cut(s) 809
BciVI GTATCC 2 cut(s) 823, 868
BcnI CCSGG 1 cut(s) 513
BcoDI GTCTC 2 cut(s) 283, 870
BcuI ACTAGT 1 cut(s) 565
BfaI CTAG 4 cut(s) 566, 656, 678, 857
BfuI GTATCC 2 cut(s) 823, 868
BglII AGATCT 1 cut(s) 744
BisI GCNGC 4 cut(s) 7, 333, 363, 462
BlsI GCNGC 4 cut(s) 8, 334, 364, 463
Bme1390I CCNGG 2 cut(s) 513, 809
Bme18I GGWCC 2 cut(s) 115, 588
BmgT120I GGNCC 3 cut(s) 115, 527, 588
BmiI GGNNCC 3 cut(s) 116, 117, 589
BmrFI CCNGG 2 cut(s) 513, 809
BmsI GCATC 2 cut(s) 50, 65
Bpu10I CCTNAGC 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 574
BpuMI CCSGG 1 cut(s) 513
BsaHI GRCGYC 1 cut(s) 257
BsaJI CCNNGG 2 cut(s) 105, 816
BsaXI ACNNNNNCTCC 2 cut(s) 731, 761
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 835
BseBI CCWGG 1 cut(s) 809
BseDI CCNNGG 2 cut(s) 105, 816
BseGI GGATG 3 cut(s) 239, 320, 521
BseLI CCNNNNNNNGG 2 cut(s) 111, 835
BseMII CTCAG 2 cut(s) 288, 444
BseXI GCAGC 3 cut(s) 344, 349, 448
BsgI GTGCAG 1 cut(s) 798
BshFI GGCC 1 cut(s) 528
BsiSI CCGG 1 cut(s) 512
BslFI GGGAC 3 cut(s) 128, 244, 601
BslI CCNNNNNNNGG 2 cut(s) 111, 835
BsmAI GTCTC 2 cut(s) 283, 870
BsmFI GGGAC 3 cut(s) 128, 244, 601
BsnI GGCC 1 cut(s) 528
Bsp143I GATC 4 cut(s) 124, 397, 522, 744
BspACI CCGC 4 cut(s) 9, 293, 560, 854
BspANI GGCC 1 cut(s) 528
BspCNI CTCAG 2 cut(s) 287, 445
BspLI GGNNCC 3 cut(s) 116, 117, 589
BspPI GGATC 1 cut(s) 392
BssECI CCNNGG 2 cut(s) 105, 816
BssMI GATC 4 cut(s) 124, 397, 522, 744
BssNAI GTATAC 1 cut(s) 849
BssNI GRCGYC 1 cut(s) 257
BssT1I CCWWGG 1 cut(s) 816
Bst1107I GTATAC 1 cut(s) 849
Bst2UI CCWGG 1 cut(s) 809
Bst4CI ACNGT 4 cut(s) 97, 109, 716, 880
BstACI GRCGYC 1 cut(s) 257
BstDEI CTNAG 2 cut(s) 274, 453
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 3 cut(s) 239, 320, 521
BstKTI GATC 4 cut(s) 127, 400, 525, 747
BstMAI GTCTC 2 cut(s) 283, 870
BstMBI GATC 4 cut(s) 124, 397, 522, 744
BstMWI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 458
BstNI CCWGG 1 cut(s) 809
BstSCI CCNGG 2 cut(s) 511, 807
BstV1I GCAGC 3 cut(s) 344, 349, 448
BstX2I RGATCY 2 cut(s) 397, 744
BstYI RGATCY 2 cut(s) 397, 744
BstZ17I GTATAC 1 cut(s) 849
BsuI GTATCC 2 cut(s) 823, 868
BsuRI GGCC 1 cut(s) 528
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 3 cut(s) 239, 320, 521
BtsIMutI CAGTG 1 cut(s) 712
CaiI CAGNNNCTG 1 cut(s) 401
Cfr13I GGNCC 3 cut(s) 115, 527, 588
CseI GACGC 1 cut(s) 246
CsiI ACCWGGT 1 cut(s) 807
Csp6I GTAC 1 cut(s) 739
CviAII CATG 1 cut(s) 60
CviQI GTAC 1 cut(s) 739
DdeI CTNAG 2 cut(s) 274, 453
DpnI GATC 4 cut(s) 126, 399, 524, 746
DpnII GATC 4 cut(s) 124, 397, 522, 744
Eco130I CCWWGG 1 cut(s) 816
Eco47I GGWCC 2 cut(s) 115, 588
Eco57I CTGAAG 1 cut(s) 890
EcoO109I RGGNCCY 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 807
EcoT14I CCWWGG 1 cut(s) 816
ErhI CCWWGG 1 cut(s) 816
FaeI CATG 1 cut(s) 63
FalI AAGNNNNNCTT 1 cut(s) 890
FaqI GGGAC 3 cut(s) 128, 244, 601
FatI CATG 1 cut(s) 59
FblI GTMKAC 1 cut(s) 848
Fnu4HI GCNGC 4 cut(s) 7, 333, 363, 462
FokI GGATG 3 cut(s) 246, 307, 528
Fsp4HI GCNGC 4 cut(s) 7, 333, 363, 462
FspBI CTAG 4 cut(s) 566, 656, 678, 857
GluI GCNGC 4 cut(s) 7, 333, 363, 462
HaeIII GGCC 1 cut(s) 528
HapII CCGG 1 cut(s) 512
HgaI GACGC 1 cut(s) 246
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 422
HinfI GANTC 1 cut(s) 383
HpaII CCGG 1 cut(s) 512
HphI GGTGA 2 cut(s) 79, 532
Hpy166II GTNNAC 2 cut(s) 683, 849
Hpy188I TCNGA 5 cut(s) 397, 549, 573, 721, 870
Hpy188III TCNNGA 2 cut(s) 113, 767
Hpy8I GTNNAC 2 cut(s) 683, 849
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 2 cut(s) 200, 865
HpyCH4III ACNGT 4 cut(s) 97, 109, 716, 880
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 4 cut(s) 82, 476, 647, 779
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 458
HpyF3I CTNAG 2 cut(s) 274, 453
HpySE526I ACGT 1 cut(s) 685
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 1 cut(s) 63
KflI GGGWCCC 1 cut(s) 115
Kzo9I GATC 4 cut(s) 124, 397, 522, 744
LmnI GCTCC 2 cut(s) 38, 161
LpnPI CCDG 9 cut(s) 30, 50, 276, 414, 525, 765, 780, 794, 821
Lsp1109I GCAGC 3 cut(s) 344, 349, 448
LweI GCATC 2 cut(s) 50, 65
MabI ACCWGGT 1 cut(s) 807
MaeI CTAG 4 cut(s) 566, 656, 678, 857
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 1 cut(s) 31
MalI GATC 4 cut(s) 126, 399, 524, 746
MboI GATC 4 cut(s) 124, 397, 522, 744
MboII GAAGA 4 cut(s) 215, 755, 898, 904
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 2 cut(s) 397, 744
MluCI AATT 2 cut(s) 225, 489
MmeI TCCRAC 2 cut(s) 319, 679
MnlI CCTC 6 cut(s) 80, 334, 366, 448, 540, 715
MroXI GAANNNNTTC 4 cut(s) 174, 567, 597, 905
MseI TTAA 1 cut(s) 345
MspI CCGG 1 cut(s) 512
MspR9I CCNGG 2 cut(s) 513, 809
MunI CAATTG 1 cut(s) 225
MvaI CCWGG 1 cut(s) 809
MwoI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 458
NciI CCSGG 1 cut(s) 513
NdeII GATC 4 cut(s) 124, 397, 522, 744
NlaIII CATG 1 cut(s) 63
NlaIV GGNNCC 3 cut(s) 116, 117, 589
NmuCI GTSAC 1 cut(s) 31
PdmI GAANNNNTTC 4 cut(s) 174, 567, 597, 905
PfeI GAWTC 1 cut(s) 383
PflMI CCANNNNNTGG 1 cut(s) 835
PfoI TCCNGGA 1 cut(s) 511
PkrI GCNGC 4 cut(s) 8, 334, 364, 463
PpuMI RGGWCCY 1 cut(s) 115
PsiI TTATAA 1 cut(s) 317
Psp5II RGGWCCY 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 807
PspGI CCWGG 1 cut(s) 807
PspN4I GGNNCC 3 cut(s) 116, 117, 589
PspPI GGNCC 3 cut(s) 115, 527, 588
PspPPI RGGWCCY 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 401
PsuI RGATCY 2 cut(s) 397, 744
RsaI GTAC 1 cut(s) 740
RsaNI GTAC 1 cut(s) 739
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 4 cut(s) 7, 333, 363, 462
Sau3AI GATC 4 cut(s) 124, 397, 522, 744
Sau96I GGNCC 3 cut(s) 115, 527, 588
ScrFI CCNGG 2 cut(s) 513, 809
SexAI ACCWGGT 1 cut(s) 807
SfaNI GCATC 2 cut(s) 50, 65
SinI GGWCC 2 cut(s) 115, 588
SmlI CTYRAG 1 cut(s) 553
SmoI CTYRAG 1 cut(s) 553
SpeI ACTAGT 1 cut(s) 565
Sse9I AATT 2 cut(s) 225, 489
SsiI CCGC 4 cut(s) 9, 293, 560, 854
SspMI CTAG 4 cut(s) 566, 656, 678, 857
StyD4I CCNGG 2 cut(s) 511, 807
StyI CCWWGG 1 cut(s) 816
TaaI ACNGT 4 cut(s) 97, 109, 716, 880
TaiI ACGT 1 cut(s) 688
TaqI TCGA 1 cut(s) 378
TaqII GACCGA 1 cut(s) 605
TasI AATT 2 cut(s) 225, 489
TfiI GAWTC 1 cut(s) 383
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 1 cut(s) 719
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 4 cut(s) 6, 332, 362, 461
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 2 cut(s) 40, 167
TspRI CASTG 1 cut(s) 719
Van91I CCANNNNNTGG 1 cut(s) 835
VpaK11BI GGWCC 2 cut(s) 115, 588
XmiI GTMKAC 1 cut(s) 848
XmnI GAANNNNTTC 4 cut(s) 174, 567, 597, 905
XspI CTAG 4 cut(s) 566, 656, 678, 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.