Rh1DG045800

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000539
Physical Location & Seq
Reverse (-)
7480800 .. 7482738
1939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG045800.1

Sequence Viewer

Length: 843 bp
ATGAGCTGTGGAGAAAGCAAGGTTGTGTGTGTGACGGGAGCATCAGGTTTCATAGCATCATGGCTGGTGAAGCTCTTATTGCAACGAGGTTATACTGTCAAAGCCACAGTTCGGGACCCAGACGATCAAAAGAAAACAGAACACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGGTCTTTTTATACGGCAATTGATGGATGTGAATGTGTTTTCCATACGGCGTCCCCTGTCCTACGCTCAGTCTCAGCAACTAATCCGCAGGCAGAATTAATTGAGCCTGCTTTGAAAGGAACGCTTAATGTCCTCGGATCGTGTGTGAAGGTTCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATGGCAGCAGTTGGATTTAATGGAAAACCTCTTGCTGCTGATGTAATAATCGATGAATCTTGGTTTTCAGATCCTGCTTTTTGTGAAAAAACAAAGCTTTGGTATATGCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAAAAAGGAATTGATATTATTACAATAAATCCGGGATGGGTGATCGGCCCTCTCTTACAGCCAACTCTGAACTTGAGTGCGGAACTAGTTCTGAAACTCGTAAATGGGACCGAAACGTTTCCCAACAGAACTTACAGACTTGTTGATGTTAGAGATGTTGCTAATGCACATATTCTAGCCCTTGAAAACCCATCAGCTAGTGGACGGTATTGTTTAGTTGGAAGCGTAAAACACTGTTCAGAGGTTGTGAAAATGTTGTACGAGATCTCCCCTGCTCTCAATCTTCCAGATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

280

Amino Acids

30.54

Weight (kDa)

6.53

Isoelectric Point (pI)

35.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 87 1.1e-08 NmrA-like family
Epimerase PF01370 9 - 249 1.4e-25 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 245 6e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 190 4.2e-13 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 202 2.7e-12 Male sterility protein
NAD_binding_10 PF13460 13 - 135 1e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 383
AciI CCGC 2 cut(s) 293, 626
AclI AACGTT 1 cut(s) 662
AclWI GGATC 2 cut(s) 352, 458
AcyI GRCGYC 1 cut(s) 257
AfaI GTAC 1 cut(s) 806
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 4 cut(s) 187, 322, 507, 731
AhlI ACTAGT 1 cut(s) 631
AluBI AGCT 6 cut(s) 6, 73, 490, 518, 527, 743
AluI AGCT 6 cut(s) 6, 73, 490, 518, 527, 743
Alw26I GTCTC 1 cut(s) 283
AlwI GGATC 2 cut(s) 352, 458
AlwNI CAGNNNCTG 1 cut(s) 467
AoxI GGCC 1 cut(s) 592
ApeKI GCWGC 3 cut(s) 398, 428, 527
AseI ATTAAT 1 cut(s) 305
Asp700I GAANNNNTTC 3 cut(s) 174, 633, 663
AspS9I GGNCC 3 cut(s) 115, 593, 654
AsuC2I CCSGG 1 cut(s) 579
AsuHPI GGTGA 2 cut(s) 79, 598
AvaII GGWCC 2 cut(s) 115, 654
BbvCI CCTCAGC 1 cut(s) 519
BbvI GCAGC 3 cut(s) 410, 415, 514
BccI CCATC 4 cut(s) 152, 224, 576, 745
BceAI ACGGC 2 cut(s) 237, 270
BcnI CCSGG 1 cut(s) 579
BcoDI GTCTC 1 cut(s) 283
BcuI ACTAGT 1 cut(s) 631
BfaI CTAG 3 cut(s) 632, 722, 744
BglII AGATCT 1 cut(s) 810
BisI GCNGC 3 cut(s) 399, 429, 528
BlsI GCNGC 3 cut(s) 400, 430, 529
Bme1390I CCNGG 1 cut(s) 579
Bme18I GGWCC 2 cut(s) 115, 654
BmgT120I GGNCC 3 cut(s) 115, 593, 654
BmiI GGNNCC 3 cut(s) 116, 117, 655
BmrFI CCNGG 1 cut(s) 579
BmsI GCATC 2 cut(s) 50, 65
Bpu10I CCTNAGC 1 cut(s) 519
BpuEI CTTGAG 1 cut(s) 640
BpuMI CCSGG 1 cut(s) 579
Bsa29I ATCGAT 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 257
BsaJI CCNNGG 1 cut(s) 340
BsaXI ACNNNNNCTCC 2 cut(s) 797, 827
Bsc4I CCNNNNNNNGG 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 340
BseGI GGATG 3 cut(s) 239, 386, 587
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 3 cut(s) 288, 294, 510
BseXI GCAGC 3 cut(s) 410, 415, 514
BshFI GGCC 1 cut(s) 594
BshVI ATCGAT 1 cut(s) 444
BsiSI CCGG 1 cut(s) 578
BslFI GGGAC 3 cut(s) 128, 244, 667
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 1 cut(s) 283
BsmFI GGGAC 3 cut(s) 128, 244, 667
BsnI GGCC 1 cut(s) 594
Bsp143I GATC 5 cut(s) 124, 344, 463, 588, 810
BspACI CCGC 2 cut(s) 293, 626
BspANI GGCC 1 cut(s) 594
BspCNI CTCAG 3 cut(s) 287, 293, 511
BspDI ATCGAT 1 cut(s) 444
BspLI GGNNCC 3 cut(s) 116, 117, 655
BspPI GGATC 2 cut(s) 352, 458
BssECI CCNNGG 1 cut(s) 340
BssMI GATC 5 cut(s) 124, 344, 463, 588, 810
BssNI GRCGYC 1 cut(s) 257
Bst4CI ACNGT 4 cut(s) 97, 109, 753, 782
BstACI GRCGYC 1 cut(s) 257
BstC8I GCNNGC 2 cut(s) 297, 315
BstDEI CTNAG 3 cut(s) 274, 280, 519
BstF5I GGATG 3 cut(s) 239, 386, 587
BstKTI GATC 5 cut(s) 127, 347, 466, 591, 813
BstMAI GTCTC 1 cut(s) 283
BstMBI GATC 5 cut(s) 124, 344, 463, 588, 810
BstMWI GCNNNNNNNGC 3 cut(s) 70, 79, 524
BstSCI CCNGG 1 cut(s) 577
BstV1I GCAGC 3 cut(s) 410, 415, 514
BstX2I RGATCY 2 cut(s) 463, 810
BstYI RGATCY 2 cut(s) 463, 810
Bsu15I ATCGAT 1 cut(s) 444
BsuRI GGCC 1 cut(s) 594
BsuTUI ATCGAT 1 cut(s) 444
BtsCI GGATG 3 cut(s) 239, 386, 587
BtsIMutI CAGTG 1 cut(s) 778
Cac8I GCNNGC 2 cut(s) 297, 315
CaiI CAGNNNCTG 1 cut(s) 467
Cfr13I GGNCC 3 cut(s) 115, 593, 654
ClaI ATCGAT 1 cut(s) 444
CseI GACGC 1 cut(s) 246
Csp6I GTAC 1 cut(s) 805
CviAII CATG 1 cut(s) 60
CviQI GTAC 1 cut(s) 805
DdeI CTNAG 3 cut(s) 274, 280, 519
DpnI GATC 5 cut(s) 126, 346, 465, 590, 812
DpnII GATC 5 cut(s) 124, 344, 463, 588, 810
Eco47I GGWCC 2 cut(s) 115, 654
EcoO109I RGGNCCY 1 cut(s) 115
FaeI CATG 1 cut(s) 63
FalI AAGNNNNNCTT 2 cut(s) 315, 347
FaqI GGGAC 3 cut(s) 128, 244, 667
FatI CATG 1 cut(s) 59
Fnu4HI GCNGC 3 cut(s) 399, 429, 528
FokI GGATG 3 cut(s) 246, 373, 594
Fsp4HI GCNGC 3 cut(s) 399, 429, 528
FspBI CTAG 3 cut(s) 632, 722, 744
GluI GCNGC 3 cut(s) 399, 429, 528
HaeIII GGCC 1 cut(s) 594
HapII CCGG 1 cut(s) 578
HgaI GACGC 1 cut(s) 246
Hin1I GRCGYC 1 cut(s) 257
Hin1II CATG 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 488
HinfI GANTC 1 cut(s) 449
HpaII CCGG 1 cut(s) 578
HphI GGTGA 2 cut(s) 79, 598
Hpy166II GTNNAC 1 cut(s) 749
Hpy188I TCNGA 5 cut(s) 344, 463, 615, 639, 787
Hpy188III TCNNGA 2 cut(s) 113, 833
Hpy8I GTNNAC 1 cut(s) 749
HpyAV CCTTC 2 cut(s) 200, 349
HpyCH4III ACNGT 4 cut(s) 97, 109, 753, 782
HpyCH4IV ACGT 1 cut(s) 662
HpyCH4V TGCA 3 cut(s) 82, 542, 713
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 79, 524
HpyF3I CTNAG 3 cut(s) 274, 280, 519
HpySE526I ACGT 1 cut(s) 662
Hsp92I GRCGYC 1 cut(s) 257
Hsp92II CATG 1 cut(s) 63
KflI GGGWCCC 1 cut(s) 115
Kzo9I GATC 5 cut(s) 124, 344, 463, 588, 810
LmnI GCTCC 2 cut(s) 38, 161
LpnPI CCDG 9 cut(s) 30, 50, 132, 276, 281, 327, 480, 591, 831
Lsp1109I GCAGC 3 cut(s) 410, 415, 514
LweI GCATC 2 cut(s) 50, 65
MaeI CTAG 3 cut(s) 632, 722, 744
MaeII ACGT 1 cut(s) 662
MaeIII GTNAC 1 cut(s) 31
MalI GATC 5 cut(s) 126, 346, 465, 590, 812
MboI GATC 5 cut(s) 124, 344, 463, 588, 810
MboII GAAGA 2 cut(s) 215, 821
MfeI CAATTG 1 cut(s) 225
MflI RGATCY 2 cut(s) 463, 810
MluCI AATT 4 cut(s) 225, 302, 306, 555
MmeI TCCRAC 2 cut(s) 385, 745
MnlI CCTC 7 cut(s) 80, 350, 400, 432, 514, 606, 781
MroXI GAANNNNTTC 3 cut(s) 174, 633, 663
MseI TTAA 3 cut(s) 305, 333, 411
MspI CCGG 1 cut(s) 578
MspR9I CCNGG 1 cut(s) 579
MunI CAATTG 1 cut(s) 225
MwoI GCNNNNNNNGC 3 cut(s) 70, 79, 524
NciI CCSGG 1 cut(s) 579
NdeII GATC 5 cut(s) 124, 344, 463, 588, 810
NlaIII CATG 1 cut(s) 63
NlaIV GGNNCC 3 cut(s) 116, 117, 655
NmuCI GTSAC 1 cut(s) 31
PdmI GAANNNNTTC 3 cut(s) 174, 633, 663
PfeI GAWTC 1 cut(s) 449
PfoI TCCNGGA 1 cut(s) 577
PkrI GCNGC 3 cut(s) 400, 430, 529
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 305
PsiI TTATAA 1 cut(s) 383
Psp1406I AACGTT 1 cut(s) 662
Psp5II RGGWCCY 1 cut(s) 115
PspN4I GGNNCC 3 cut(s) 116, 117, 655
PspPI GGNCC 3 cut(s) 115, 593, 654
PspPPI RGGWCCY 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 467
PsuI RGATCY 2 cut(s) 463, 810
RsaI GTAC 1 cut(s) 806
RsaNI GTAC 1 cut(s) 805
SaqAI TTAA 3 cut(s) 305, 333, 411
SatI GCNGC 3 cut(s) 399, 429, 528
Sau3AI GATC 5 cut(s) 124, 344, 463, 588, 810
Sau96I GGNCC 3 cut(s) 115, 593, 654
ScrFI CCNGG 1 cut(s) 579
SfaNI GCATC 2 cut(s) 50, 65
SinI GGWCC 2 cut(s) 115, 654
SmlI CTYRAG 1 cut(s) 619
SmoI CTYRAG 1 cut(s) 619
SpeI ACTAGT 1 cut(s) 631
Sse9I AATT 4 cut(s) 225, 302, 306, 555
SsiI CCGC 2 cut(s) 293, 626
SspMI CTAG 3 cut(s) 632, 722, 744
StyD4I CCNGG 1 cut(s) 577
TaaI ACNGT 4 cut(s) 97, 109, 753, 782
TaiI ACGT 1 cut(s) 665
TaqI TCGA 1 cut(s) 444
TaqII GACCGA 1 cut(s) 671
TasI AATT 4 cut(s) 225, 302, 306, 555
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 3 cut(s) 305, 333, 411
Tru9I TTAA 3 cut(s) 305, 333, 411
TscAI CASTG 1 cut(s) 785
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 3 cut(s) 398, 428, 527
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 3 cut(s) 40, 167, 462
TspRI CASTG 1 cut(s) 785
VpaK11BI GGWCC 2 cut(s) 115, 654
VspI ATTAAT 1 cut(s) 305
XmnI GAANNNNTTC 3 cut(s) 174, 633, 663
XspI CTAG 3 cut(s) 632, 722, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.