Rroxscaffold_4G00330250

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000539
Physical Location & Seq
Forward (+)
63758834 .. 63761638
2805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00330250.1

Sequence Viewer

Length: 1011 bp
ATGAGCTGCGGAGTAAGCAAGGTTGTGTGTGTGACAGGAGCATCTGGGTTCATAGCATCATGGCTAGTCAAGCGCTTATTGCAACGAGGTTATACTGTCAAAGCCACTGTTCGGGACCCAAATGATCACAAGAAAACAGAACACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGAATTGTTAGACGAAGGTTCTTTTGATCCTGTCGTTGATGGGTGTGAAGGTGTTTTTCATACAGCATCCCCTGCCCTACTCTCATCTACTAATCCGCAGGCAGAATTAATTGAGCCTGCTTTGAAGGGAACGCTTAATGTCCTTGGATCGTGTGTGAAGGTTCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATGGCAGCAGTTGGATTTAATGGAAAACCTCTTGCTGCTGATGTAATAATCGATGAATCTTGGTTTTCAGATCCTGCTTTTTGTGAAAAAACGAAGCTTTGGTATATGCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAAAAAGGAATTGATATTATTACAATAAATCCGGGATGGGTGATCGGCCCCTCTCTTACAACCAACTCTGAACTTGAGTGTGGAACTAGTTCTGAAACTCATAAATGCCTGGTAGCTTTGTGTTATAAACTTAAAGTGAAAGTTGGGACCGAAAAGTTTCCCAACAAAACTTACAGACTTGTTGATGTTAGAGATGTTGCTATTGCACATATTCTAGCCTTTGAAAACCCATCAGCTAGTGGACGTTATTGTTTAGTTGGAAGCGTAAAACACTGTTCAGAGGTTGTGAAAATGTTGTACGAGATCTCCCCTGCTCTCAATCTTCCAGATAAATGTGCAGATGACAAGCCTTTCACACCAACCTACCGGGTATCCAAGGAAAGAACCCAAACTTTGGGTGTAAAGTATACTCCGCTTGAAGTGTCTCTGAAGGATACTGTTGAAAGTTTGAAGAACAAGAACTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

336

Amino Acids

36.85

Weight (kDa)

8.38

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 101 5.1e-08 NmrA-like family
Epimerase PF01370 9 - 260 4.5e-24 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 254 5.2e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 11 - 223 2e-12 Male sterility protein
GDP_Man_Dehyd PF16363 11 - 132 6.7e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 133 2.5e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 377, 666
AccB7I CCANNNNNTGG 1 cut(s) 934
AccI GTMKAC 1 cut(s) 947
AciI CCGC 3 cut(s) 9, 287, 953
AclWI GGATC 3 cut(s) 212, 346, 452
AcuI CTGAAG 1 cut(s) 989
AfaI GTAC 1 cut(s) 839
AfeI AGCGCT 1 cut(s) 74
AfiI CCNNNNNNNGG 2 cut(s) 111, 934
AgsI TTSAA 7 cut(s) 187, 316, 501, 764, 959, 983, 991
AhlI ACTAGT 1 cut(s) 626
AjnI CCWGG 1 cut(s) 648
AluBI AGCT 6 cut(s) 6, 484, 512, 521, 656, 776
AluI AGCT 6 cut(s) 6, 484, 512, 521, 656, 776
Alw26I GTCTC 1 cut(s) 969
AlwI GGATC 3 cut(s) 212, 346, 452
AlwNI CAGNNNCTG 1 cut(s) 461
Aor51HI AGCGCT 1 cut(s) 74
AoxI GGCC 1 cut(s) 586
ApeKI GCWGC 4 cut(s) 6, 392, 422, 521
AseI ATTAAT 1 cut(s) 299
Asp700I GAANNNNTTC 4 cut(s) 174, 628, 696, 1004
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 3 cut(s) 115, 587, 687
AsuC2I CCSGG 2 cut(s) 573, 908
AsuHPI GGTGA 1 cut(s) 592
AvaII GGWCC 2 cut(s) 115, 687
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 3 cut(s) 404, 409, 508
BccI CCATC 4 cut(s) 152, 224, 570, 778
BciT130I CCWGG 1 cut(s) 650
BciVI GTATCC 2 cut(s) 922, 967
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 2 cut(s) 573, 908
BcoDI GTCTC 1 cut(s) 969
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 4 cut(s) 65, 627, 755, 777
BfoI RGCGCY 1 cut(s) 76
BfuI GTATCC 2 cut(s) 922, 967
BglII AGATCT 1 cut(s) 843
BisI GCNGC 4 cut(s) 7, 393, 423, 522
BlsI GCNGC 4 cut(s) 8, 394, 424, 523
Bme1390I CCNGG 3 cut(s) 573, 650, 908
Bme18I GGWCC 2 cut(s) 115, 687
BmgT120I GGNCC 3 cut(s) 115, 587, 687
BmiI GGNNCC 4 cut(s) 116, 117, 589, 688
BmrFI CCNGG 3 cut(s) 573, 650, 908
BmsI GCATC 3 cut(s) 50, 65, 266
Bpu10I CCTNAGC 1 cut(s) 513
BpuEI CTTGAG 1 cut(s) 635
BpuMI CCSGG 2 cut(s) 573, 908
Bsa29I ATCGAT 1 cut(s) 438
BsaJI CCNNGG 2 cut(s) 334, 915
BsaXI ACNNNNNCTCC 2 cut(s) 830, 860
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 934
BseBI CCWGG 1 cut(s) 650
BseCI ATCGAT 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 334, 915
BseGI GGATG 3 cut(s) 257, 380, 581
BseLI CCNNNNNNNGG 2 cut(s) 111, 934
BseMII CTCAG 1 cut(s) 504
BseXI GCAGC 3 cut(s) 404, 409, 508
BsgI GTGCAG 1 cut(s) 897
BshFI GGCC 1 cut(s) 588
BshVI ATCGAT 1 cut(s) 438
BsiSI CCGG 2 cut(s) 572, 907
BslFI GGGAC 2 cut(s) 128, 700
BslI CCNNNNNNNGG 2 cut(s) 111, 934
BsmAI GTCTC 1 cut(s) 969
BsmFI GGGAC 2 cut(s) 128, 700
BsnI GGCC 1 cut(s) 588
Bsp143I GATC 6 cut(s) 124, 217, 338, 457, 582, 843
BspACI CCGC 3 cut(s) 9, 287, 953
BspANI GGCC 1 cut(s) 588
BspCNI CTCAG 1 cut(s) 505
BspDI ATCGAT 1 cut(s) 438
BspLI GGNNCC 4 cut(s) 116, 117, 589, 688
BspPI GGATC 3 cut(s) 212, 346, 452
BssECI CCNNGG 2 cut(s) 334, 915
BssMI GATC 6 cut(s) 124, 217, 338, 457, 582, 843
BssNAI GTATAC 1 cut(s) 948
BssT1I CCWWGG 2 cut(s) 334, 915
Bst1107I GTATAC 1 cut(s) 948
Bst2UI CCWGG 1 cut(s) 650
Bst4CI ACNGT 4 cut(s) 97, 109, 815, 979
BstAPI GCANNNNNTGC 1 cut(s) 263
BstC8I GCNNGC 2 cut(s) 291, 309
BstDEI CTNAG 1 cut(s) 513
BstF5I GGATG 3 cut(s) 257, 380, 581
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 6 cut(s) 127, 220, 341, 460, 585, 846
BstMAI GTCTC 1 cut(s) 969
BstMBI GATC 6 cut(s) 124, 217, 338, 457, 582, 843
BstMWI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 263, 518
BstNI CCWGG 1 cut(s) 650
BstSCI CCNGG 3 cut(s) 571, 648, 906
BstV1I GCAGC 3 cut(s) 404, 409, 508
BstX2I RGATCY 2 cut(s) 457, 843
BstYI RGATCY 2 cut(s) 457, 843
BstZ17I GTATAC 1 cut(s) 948
Bsu15I ATCGAT 1 cut(s) 438
BsuI GTATCC 2 cut(s) 922, 967
BsuRI GGCC 1 cut(s) 588
BsuTUI ATCGAT 1 cut(s) 438
BtsCI GGATG 3 cut(s) 257, 380, 581
BtsIMutI CAGTG 2 cut(s) 105, 811
Cac8I GCNNGC 2 cut(s) 291, 309
CaiI CAGNNNCTG 1 cut(s) 461
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 3 cut(s) 115, 587, 687
ClaI ATCGAT 1 cut(s) 438
Csp6I GTAC 1 cut(s) 838
CviAII CATG 1 cut(s) 60
CviQI GTAC 1 cut(s) 838
DdeI CTNAG 1 cut(s) 513
DpnI GATC 6 cut(s) 126, 219, 340, 459, 584, 845
DpnII GATC 6 cut(s) 124, 217, 338, 457, 582, 843
Eco130I CCWWGG 2 cut(s) 334, 915
Eco47I GGWCC 2 cut(s) 115, 687
Eco47III AGCGCT 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 989
EcoO109I RGGNCCY 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 648
EcoT14I CCWWGG 2 cut(s) 334, 915
ErhI CCWWGG 2 cut(s) 334, 915
FaeI CATG 1 cut(s) 63
FalI AAGNNNNNCTT 1 cut(s) 989
FaqI GGGAC 2 cut(s) 128, 700
FatI CATG 1 cut(s) 59
FbaI TGATCA 1 cut(s) 124
FblI GTMKAC 1 cut(s) 947
Fnu4HI GCNGC 4 cut(s) 7, 393, 423, 522
FokI GGATG 3 cut(s) 244, 367, 588
Fsp4HI GCNGC 4 cut(s) 7, 393, 423, 522
FspBI CTAG 4 cut(s) 65, 627, 755, 777
GlaI GCGC 1 cut(s) 74
GluI GCNGC 4 cut(s) 7, 393, 423, 522
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 1 cut(s) 588
HapII CCGG 2 cut(s) 572, 907
HhaI GCGC 1 cut(s) 75
Hin1II CATG 1 cut(s) 63
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 1 cut(s) 443
HpaII CCGG 2 cut(s) 572, 907
HphI GGTGA 1 cut(s) 592
Hpy166II GTNNAC 2 cut(s) 782, 948
Hpy188I TCNGA 5 cut(s) 457, 610, 634, 820, 969
Hpy188III TCNNGA 2 cut(s) 113, 866
Hpy8I GTNNAC 2 cut(s) 782, 948
HpyAV CCTTC 5 cut(s) 200, 233, 310, 343, 964
HpyCH4III ACNGT 4 cut(s) 97, 109, 815, 979
HpyCH4IV ACGT 1 cut(s) 784
HpyCH4V TGCA 4 cut(s) 82, 536, 746, 878
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 263, 518
HpyF3I CTNAG 1 cut(s) 513
HpySE526I ACGT 1 cut(s) 784
Hsp92II CATG 1 cut(s) 63
HspAI GCGC 1 cut(s) 73
KflI GGGWCCC 1 cut(s) 115
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 6 cut(s) 124, 217, 338, 457, 582, 843
LmnI GCTCC 2 cut(s) 38, 161
Lsp1109I GCAGC 3 cut(s) 404, 409, 508
LweI GCATC 3 cut(s) 50, 65, 266
MaeI CTAG 4 cut(s) 65, 627, 755, 777
MaeII ACGT 1 cut(s) 784
MaeIII GTNAC 1 cut(s) 31
MalI GATC 6 cut(s) 126, 219, 340, 459, 584, 845
MboI GATC 6 cut(s) 124, 217, 338, 457, 582, 843
MboII GAAGA 3 cut(s) 854, 997, 1003
MflI RGATCY 2 cut(s) 457, 843
MluCI AATT 4 cut(s) 194, 296, 300, 549
MmeI TCCRAC 2 cut(s) 379, 778
MnlI CCTC 6 cut(s) 80, 394, 426, 508, 601, 814
MroXI GAANNNNTTC 4 cut(s) 174, 628, 696, 1004
MseI TTAA 4 cut(s) 299, 327, 405, 672
MspI CCGG 2 cut(s) 572, 907
MspR9I CCNGG 3 cut(s) 573, 650, 908
MvaI CCWGG 1 cut(s) 650
MwoI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 263, 518
NciI CCSGG 2 cut(s) 573, 908
NdeII GATC 6 cut(s) 124, 217, 338, 457, 582, 843
NlaIII CATG 1 cut(s) 63
NlaIV GGNNCC 4 cut(s) 116, 117, 589, 688
NmuCI GTSAC 1 cut(s) 31
PdmI GAANNNNTTC 4 cut(s) 174, 628, 696, 1004
PfeI GAWTC 1 cut(s) 443
PflMI CCANNNNNTGG 1 cut(s) 934
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 4 cut(s) 8, 394, 424, 523
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 299
PsiI TTATAA 2 cut(s) 377, 666
Psp5II RGGWCCY 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 648
PspGI CCWGG 1 cut(s) 648
PspN4I GGNNCC 4 cut(s) 116, 117, 589, 688
PspPI GGNCC 3 cut(s) 115, 587, 687
PspPPI RGGWCCY 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 2 cut(s) 457, 843
RsaI GTAC 1 cut(s) 839
RsaNI GTAC 1 cut(s) 838
SaqAI TTAA 4 cut(s) 299, 327, 405, 672
SatI GCNGC 4 cut(s) 7, 393, 423, 522
Sau3AI GATC 6 cut(s) 124, 217, 338, 457, 582, 843
Sau96I GGNCC 3 cut(s) 115, 587, 687
ScrFI CCNGG 3 cut(s) 573, 650, 908
SfaNI GCATC 3 cut(s) 50, 65, 266
SinI GGWCC 2 cut(s) 115, 687
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
SpeI ACTAGT 1 cut(s) 626
Sse9I AATT 4 cut(s) 194, 296, 300, 549
SsiI CCGC 3 cut(s) 9, 287, 953
SspMI CTAG 4 cut(s) 65, 627, 755, 777
StyD4I CCNGG 3 cut(s) 571, 648, 906
StyI CCWWGG 2 cut(s) 334, 915
TaaI ACNGT 4 cut(s) 97, 109, 815, 979
TaiI ACGT 1 cut(s) 787
TaqI TCGA 1 cut(s) 438
TaqII GACCGA 1 cut(s) 704
TasI AATT 4 cut(s) 194, 296, 300, 549
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 4 cut(s) 299, 327, 405, 672
Tru9I TTAA 4 cut(s) 299, 327, 405, 672
TscAI CASTG 2 cut(s) 112, 818
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 4 cut(s) 6, 392, 422, 521
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 40, 167, 239, 456
TspRI CASTG 2 cut(s) 112, 818
Van91I CCANNNNNTGG 1 cut(s) 934
VpaK11BI GGWCC 2 cut(s) 115, 687
VspI ATTAAT 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 947
XmnI GAANNNNTTC 4 cut(s) 174, 628, 696, 1004
XspI CTAG 4 cut(s) 65, 627, 755, 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.