Rroxscaffold_4G00330260

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000539
Physical Location & Seq
Forward (+)
63806259 .. 63808810
2552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00330260.1

Sequence Viewer

Length: 870 bp
ATGAACTGCGGAGAAAGCAATGTTGTGTGTGTGACGGGAGCATCTGGTTTCATAGCATCATGGCTGGTGAAGCTCTTATTGCAAACGAGGTTATACTGTCAAAGCCACGGTTCGGGACCCAGTTTGATTTTACCTGCAAAATCTATATCAGTATGCACTATGATGTTGCTAGCTCTTAATCTTTGTCAGGCAGAATTGATTGAGCCTGCTTTGAAAGGAACGCTTAATGTCCTCGGATCGTGTGTGAAGGTTCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATAGGAGCAGTTGGATATAATGGAAAACCTCTTACTGCTGATGTAATAATCGATGAATCTTGGTTTTCAGATCCTGCTTTTTGTGAAAAAACAAAGCTTTGGTATATGCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAGAAAGGAATTGATATTATTACAATAAATCCGGGATGGGTGATCGGCCCTCTTTTACAGCCAACTCTAAACTTTAGTGATGAAATAGTTCTGAAACTCGTAAATGGGACCGAAAAGTTTCCCAACAAAACTTACGGATTTGTTGATGTTAGAGATGTTGCTAATGCCCATATTCTAGCCTTTGAAAACCTATCAGCTAGTGGACGTTATTGTTTAGTTGGAAGCGTAAAACACTGTTCAGAGGTTGTGAAAATATTGCACGAGATCTCCCCTGCTCTCAATCTTCCTGATAAATGTGCAGATGACAAGCCTTTCTCACCAGCTTACCAGGTGTCCAAGGAAAGAGCCCAAACTTTGGGGGTAAAGTATATTCCGCTTGAAGTGTCTCTGAAGGATACAGTTGAAAGTTTGAAGGACAAGAACTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

289

Amino Acids

31.55

Weight (kDa)

7.48

Isoelectric Point (pI)

29.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 65 - 212 1.5e-09 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 65 - 213 1.3e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 275
Acc36I ACCTGC 1 cut(s) 142
AccB7I CCANNNNNTGG 1 cut(s) 793
AciI CCGC 2 cut(s) 9, 812
AclWI GGATC 2 cut(s) 244, 350
AcuI CTGAAG 1 cut(s) 848
AfiI CCNNNNNNNGG 2 cut(s) 112, 793
AgsI TTSAA 6 cut(s) 214, 399, 623, 818, 842, 850
AjnI CCWGG 1 cut(s) 765
AluBI AGCT 7 cut(s) 73, 173, 382, 410, 419, 635, 761
AluI AGCT 7 cut(s) 73, 173, 382, 410, 419, 635, 761
Alw26I GTCTC 1 cut(s) 828
AlwI GGATC 2 cut(s) 244, 350
AlwNI CAGNNNCTG 1 cut(s) 359
AoxI GGCC 1 cut(s) 484
ApeKI GCWGC 1 cut(s) 419
Asp700I GAANNNNTTC 3 cut(s) 525, 555, 863
AspS9I GGNCC 3 cut(s) 116, 485, 546
AsuC2I CCSGG 1 cut(s) 471
AsuHPI GGTGA 3 cut(s) 79, 490, 747
AsuNHI GCTAGC 1 cut(s) 169
AvaII GGWCC 2 cut(s) 116, 546
BanII GRGCYC 1 cut(s) 787
BauI CACGAG 1 cut(s) 698
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 1 cut(s) 406
BccI CCATC 1 cut(s) 468
BciT130I CCWGG 1 cut(s) 767
BciVI GTATCC 1 cut(s) 826
BcnI CCSGG 1 cut(s) 471
BcoDI GTCTC 1 cut(s) 828
BfaI CTAG 3 cut(s) 170, 614, 636
BfmI CTRYAG 1 cut(s) 285
BfuAI ACCTGC 1 cut(s) 142
BfuI GTATCC 1 cut(s) 826
BglII AGATCT 1 cut(s) 702
BisI GCNGC 1 cut(s) 420
BlsI GCNGC 1 cut(s) 421
Bme1390I CCNGG 2 cut(s) 471, 767
Bme18I GGWCC 2 cut(s) 116, 546
BmgT120I GGNCC 3 cut(s) 116, 485, 546
BmiI GGNNCC 3 cut(s) 117, 118, 547
BmrFI CCNGG 2 cut(s) 471, 767
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 2 cut(s) 50, 65
BmtI GCTAGC 1 cut(s) 173
BmuI ACTGGG 1 cut(s) 114
Bpu10I CCTNAGC 1 cut(s) 411
BpuMI CCSGG 1 cut(s) 471
Bsa29I ATCGAT 1 cut(s) 336
BsaJI CCNNGG 3 cut(s) 106, 232, 774
BsaXI ACNNNNNCTCC 2 cut(s) 689, 719
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 793
Bse1I ACTGG 1 cut(s) 120
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 1 cut(s) 767
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 3 cut(s) 106, 232, 774
BseGI GGATG 2 cut(s) 278, 479
BseLI CCNNNNNNNGG 2 cut(s) 112, 793
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 1 cut(s) 402
BseNI ACTGG 1 cut(s) 120
BseXI GCAGC 1 cut(s) 406
BsgI GTGCAG 1 cut(s) 756
BshFI GGCC 1 cut(s) 486
BshVI ATCGAT 1 cut(s) 336
BsiSI CCGG 1 cut(s) 470
BslFI GGGAC 2 cut(s) 129, 559
BslI CCNNNNNNNGG 2 cut(s) 112, 793
BsmAI GTCTC 1 cut(s) 828
BsmFI GGGAC 2 cut(s) 129, 559
BsnI GGCC 1 cut(s) 486
Bsp1286I GDGCHC 1 cut(s) 787
Bsp143I GATC 4 cut(s) 236, 355, 480, 702
BspACI CCGC 2 cut(s) 9, 812
BspANI GGCC 1 cut(s) 486
BspCNI CTCAG 1 cut(s) 403
BspDI ATCGAT 1 cut(s) 336
BspLI GGNNCC 3 cut(s) 117, 118, 547
BspMI ACCTGC 1 cut(s) 142
BspOI GCTAGC 1 cut(s) 173
BspPI GGATC 2 cut(s) 244, 350
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 1 cut(s) 120
BssECI CCNNGG 3 cut(s) 106, 232, 774
BssMI GATC 4 cut(s) 236, 355, 480, 702
BssSI CACGAG 1 cut(s) 698
BssT1I CCWWGG 1 cut(s) 774
Bst2BI CACGAG 1 cut(s) 698
Bst2UI CCWGG 1 cut(s) 767
Bst4CI ACNGT 4 cut(s) 98, 110, 674, 838
BstC8I GCNNGC 2 cut(s) 171, 207
BstDEI CTNAG 1 cut(s) 411
BstDSI CCRYGG 1 cut(s) 106
BstF5I GGATG 2 cut(s) 278, 479
BstKTI GATC 4 cut(s) 239, 358, 483, 705
BstMAI GTCTC 1 cut(s) 828
BstMBI GATC 4 cut(s) 236, 355, 480, 702
BstMWI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 416
BstNI CCWGG 1 cut(s) 767
BstSCI CCNGG 2 cut(s) 469, 765
BstSFI CTRYAG 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 406
BstX2I RGATCY 2 cut(s) 355, 702
BstYI RGATCY 2 cut(s) 355, 702
Bsu15I ATCGAT 1 cut(s) 336
BsuI GTATCC 1 cut(s) 826
BsuRI GGCC 1 cut(s) 486
BsuTUI ATCGAT 1 cut(s) 336
BtgI CCRYGG 1 cut(s) 106
BtsCI GGATG 2 cut(s) 278, 479
BtsIMutI CAGTG 1 cut(s) 670
BveI ACCTGC 1 cut(s) 142
Cac8I GCNNGC 2 cut(s) 171, 207
CaiI CAGNNNCTG 1 cut(s) 359
Cfr13I GGNCC 3 cut(s) 116, 485, 546
ClaI ATCGAT 1 cut(s) 336
CsiI ACCWGGT 1 cut(s) 765
CviAII CATG 1 cut(s) 60
DdeI CTNAG 1 cut(s) 411
DpnI GATC 4 cut(s) 238, 357, 482, 704
DpnII GATC 4 cut(s) 236, 355, 480, 702
Eco130I CCWWGG 1 cut(s) 774
Eco24I GRGCYC 1 cut(s) 787
Eco47I GGWCC 2 cut(s) 116, 546
Eco57I CTGAAG 1 cut(s) 848
EcoO109I RGGNCCY 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 765
EcoT14I CCWWGG 1 cut(s) 774
EcoT38I GRGCYC 1 cut(s) 787
ErhI CCWWGG 1 cut(s) 774
FaeI CATG 1 cut(s) 63
FalI AAGNNNNNCTT 3 cut(s) 207, 239, 848
FaqI GGGAC 2 cut(s) 129, 559
FatI CATG 1 cut(s) 59
Fnu4HI GCNGC 1 cut(s) 420
FokI GGATG 2 cut(s) 265, 486
FriOI GRGCYC 1 cut(s) 787
Fsp4HI GCNGC 1 cut(s) 420
FspBI CTAG 3 cut(s) 170, 614, 636
GluI GCNGC 1 cut(s) 420
HaeIII GGCC 1 cut(s) 486
HapII CCGG 1 cut(s) 470
Hin1II CATG 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 1 cut(s) 341
HpaII CCGG 1 cut(s) 470
HphI GGTGA 3 cut(s) 79, 490, 747
Hpy166II GTNNAC 1 cut(s) 641
Hpy188I TCNGA 5 cut(s) 236, 355, 531, 679, 828
Hpy188III TCNNGA 2 cut(s) 114, 725
Hpy8I GTNNAC 1 cut(s) 641
HpyAV CCTTC 3 cut(s) 241, 823, 844
HpyCH4III ACNGT 4 cut(s) 98, 110, 674, 838
HpyCH4IV ACGT 1 cut(s) 643
HpyCH4V TGCA 6 cut(s) 82, 137, 156, 434, 697, 737
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 416
HpyF3I CTNAG 1 cut(s) 411
HpySE526I ACGT 1 cut(s) 643
Hsp92II CATG 1 cut(s) 63
KflI GGGWCCC 1 cut(s) 116
Kzo9I GATC 4 cut(s) 236, 355, 480, 702
LmnI GCTCC 2 cut(s) 38, 290
Lsp1109I GCAGC 1 cut(s) 406
LweI GCATC 2 cut(s) 50, 65
MabI ACCWGGT 1 cut(s) 765
MaeI CTAG 3 cut(s) 170, 614, 636
MaeII ACGT 1 cut(s) 643
MaeIII GTNAC 1 cut(s) 31
MalI GATC 4 cut(s) 238, 357, 482, 704
MboI GATC 4 cut(s) 236, 355, 480, 702
MboII GAAGA 2 cut(s) 713, 856
MflI RGATCY 2 cut(s) 355, 702
MhlI GDGCHC 1 cut(s) 787
MluCI AATT 2 cut(s) 194, 447
MmeI TCCRAC 2 cut(s) 277, 637
MnlI CCTC 7 cut(s) 81, 242, 292, 324, 406, 498, 673
MroXI GAANNNNTTC 3 cut(s) 525, 555, 863
MseI TTAA 2 cut(s) 177, 225
MslI CAYNNNNRTG 1 cut(s) 161
MspI CCGG 1 cut(s) 470
MspR9I CCNGG 2 cut(s) 471, 767
MvaI CCWGG 1 cut(s) 767
MwoI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 416
NciI CCSGG 1 cut(s) 471
NdeII GATC 4 cut(s) 236, 355, 480, 702
NheI GCTAGC 1 cut(s) 169
NlaIII CATG 1 cut(s) 63
NlaIV GGNNCC 3 cut(s) 117, 118, 547
NmuCI GTSAC 1 cut(s) 31
PdmI GAANNNNTTC 3 cut(s) 525, 555, 863
PfeI GAWTC 1 cut(s) 341
PflMI CCANNNNNTGG 1 cut(s) 793
PfoI TCCNGGA 1 cut(s) 469
PkrI GCNGC 1 cut(s) 421
PpuMI RGGWCCY 1 cut(s) 116
PsiI TTATAA 1 cut(s) 275
Psp5II RGGWCCY 1 cut(s) 116
Psp6I CCWGG 1 cut(s) 765
PspGI CCWGG 1 cut(s) 765
PspN4I GGNNCC 3 cut(s) 117, 118, 547
PspPI GGNCC 3 cut(s) 116, 485, 546
PspPPI RGGWCCY 1 cut(s) 116
PstNI CAGNNNCTG 1 cut(s) 359
PsuI RGATCY 2 cut(s) 355, 702
RseI CAYNNNNRTG 1 cut(s) 161
SaqAI TTAA 2 cut(s) 177, 225
SatI GCNGC 1 cut(s) 420
Sau3AI GATC 4 cut(s) 236, 355, 480, 702
Sau96I GGNCC 3 cut(s) 116, 485, 546
ScrFI CCNGG 2 cut(s) 471, 767
SduI GDGCHC 1 cut(s) 787
SexAI ACCWGGT 1 cut(s) 765
SfaNI GCATC 2 cut(s) 50, 65
SfcI CTRYAG 1 cut(s) 285
SinI GGWCC 2 cut(s) 116, 546
SmiMI CAYNNNNRTG 1 cut(s) 161
Sse9I AATT 2 cut(s) 194, 447
SsiI CCGC 2 cut(s) 9, 812
SspI AATATT 1 cut(s) 693
SspMI CTAG 3 cut(s) 170, 614, 636
StyD4I CCNGG 2 cut(s) 469, 765
StyI CCWWGG 1 cut(s) 774
TaaI ACNGT 4 cut(s) 98, 110, 674, 838
TaiI ACGT 1 cut(s) 646
TaqI TCGA 1 cut(s) 336
TaqII GACCGA 1 cut(s) 563
TasI AATT 2 cut(s) 194, 447
TfiI GAWTC 1 cut(s) 341
Tru1I TTAA 2 cut(s) 177, 225
Tru9I TTAA 2 cut(s) 177, 225
TscAI CASTG 1 cut(s) 677
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 1 cut(s) 419
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 17, 40, 354, 534
TspGWI ACGGA 1 cut(s) 588
TspRI CASTG 1 cut(s) 677
Van91I CCANNNNNTGG 1 cut(s) 793
VpaK11BI GGWCC 2 cut(s) 116, 546
XmnI GAANNNNTTC 3 cut(s) 525, 555, 863
XspI CTAG 3 cut(s) 170, 614, 636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.