Rh1DG044500

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0000539
Physical Location & Seq
Forward (+)
7261905 .. 7281487
19583 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG044500.1

Sequence Viewer

Length: 840 bp
ATGAGCTGCGGAGAAAGCAAGGTTGTGTGTGTGACAGGAGCATCTGGATTCATAGCATCATGGCTGGTGAAGCTTTTATTGCAAAGAGGTTTTACTGTCAAAGCCACTGTTCGTGACCCAAATGATCAAAAGAAAACAGATCACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGTTCTTTTGATTCTGTAATTGATGGATGCGAATGTGTTTTCCATGTGGCATCCCCTGTAATACTTTCAGACCTCACTGACCCCCAGGCAGAATTACTTGACCCTGCGTTGAAGGGAACACTTAATGTCCTTGGATCCTGTGTGAAGGTTCCGTCTACCAAAAGGGTGGTTATAACATCCTCCATGGCAGCAGTTGCATTTACTGGAAAACCTCTTTCTGCTGATGTAATAATCGACGAATCTTGGTTTTCAGATCCTGCTTTTTGTGAAGAAACAAAGCTTTGGTATATGCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAGAAAGGAATTGATATAATTACAATAAATCCCGGATTGGCGATCGGCCCTCTCTTACAGCCAACTCTCAACACAAGTGTGCAGCCAATTCTAAAACTCGTAGATGGGACTGAAGAATTTACTAACACAACTTACATGCTCATTGATGTTAGAGATGTTGCCAATGCACATATTCTGGCCTTTGACAACCCATCAGCTAGTGGACGTTATTGTTTGGTTGAAAGAGTAAAACACTGTTCAGAGGTTGTGAAAATGTTGAGCGATATCTCCCCTGCTCTTAATCTTCCAGATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

279

Amino Acids

30.31

Weight (kDa)

5.1

Isoelectric Point (pI)

37.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 106 2.3e-09 NmrA-like family
Epimerase PF01370 9 - 248 2.4e-22 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 242 3.3e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 11 - 201 8.6e-12 Male sterility protein
GDP_Man_Dehyd PF16363 11 - 130 1e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 134 1e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 380
AccI GTMKAC 1 cut(s) 362
AciI CCGC 1 cut(s) 9
AclWI GGATC 3 cut(s) 336, 349, 455
AcsI RAATTY 1 cut(s) 659
AcuI CTGAAG 1 cut(s) 675
AgsI TTSAA 4 cut(s) 187, 319, 504, 764
AjnI CCWGG 1 cut(s) 291
AleI CACNNNNGTG 1 cut(s) 620
AluBI AGCT 6 cut(s) 6, 73, 487, 515, 524, 740
AluI AGCT 6 cut(s) 6, 73, 487, 515, 524, 740
AlwI GGATC 3 cut(s) 336, 349, 455
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 2 cut(s) 589, 720
ApeKI GCWGC 4 cut(s) 6, 395, 524, 625
ApoI RAATTY 1 cut(s) 659
Asp700I GAANNNNTTC 1 cut(s) 174
AspS9I GGNCC 1 cut(s) 590
AsuC2I CCSGG 1 cut(s) 576
AsuHPI GGTGA 2 cut(s) 79, 134
BamHI GGATCC 1 cut(s) 341
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 3 cut(s) 407, 511, 637
BccI CCATC 4 cut(s) 152, 224, 641, 742
BciT130I CCWGG 1 cut(s) 293
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 1 cut(s) 576
BfaI CTAG 1 cut(s) 741
BisI GCNGC 4 cut(s) 7, 396, 525, 626
BlsI GCNGC 4 cut(s) 8, 397, 526, 627
Bme1390I CCNGG 2 cut(s) 293, 576
BmgT120I GGNCC 1 cut(s) 590
BmiI GGNNCC 2 cut(s) 343, 357
BmrFI CCNGG 2 cut(s) 293, 576
BmsI GCATC 4 cut(s) 50, 65, 224, 266
Bpu10I CCTNAGC 1 cut(s) 516
BpuMI CCSGG 1 cut(s) 576
BsaJI CCNNGG 3 cut(s) 291, 337, 390
Bse1I ACTGG 1 cut(s) 415
BseBI CCWGG 1 cut(s) 293
BseDI CCNNGG 3 cut(s) 291, 337, 390
BseGI GGATG 3 cut(s) 239, 257, 383
BseMII CTCAG 1 cut(s) 507
BseNI ACTGG 1 cut(s) 415
BseXI GCAGC 3 cut(s) 407, 511, 637
BsgI GTGCAG 1 cut(s) 644
Bsh1285I CGRYCG 1 cut(s) 588
BshFI GGCC 2 cut(s) 591, 722
BsiEI CGRYCG 1 cut(s) 588
BsiSI CCGG 1 cut(s) 576
BslFI GGGAC 1 cut(s) 664
BsmFI GGGAC 1 cut(s) 664
BsnI GGCC 2 cut(s) 591, 722
Bsp143I GATC 5 cut(s) 124, 139, 341, 460, 585
Bsp19I CCATGG 1 cut(s) 390
BspACI CCGC 1 cut(s) 9
BspANI GGCC 2 cut(s) 591, 722
BspCNI CTCAG 1 cut(s) 508
BspLI GGNNCC 2 cut(s) 343, 357
BspPI GGATC 3 cut(s) 336, 349, 455
BsrI ACTGG 1 cut(s) 415
BssECI CCNNGG 3 cut(s) 291, 337, 390
BssMI GATC 5 cut(s) 124, 139, 341, 460, 585
BssT1I CCWWGG 2 cut(s) 337, 390
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 3 cut(s) 97, 109, 779
BstAPI GCANNNNNTGC 1 cut(s) 401
BstDEI CTNAG 1 cut(s) 516
BstDSI CCRYGG 1 cut(s) 390
BstF5I GGATG 3 cut(s) 239, 257, 383
BstKTI GATC 5 cut(s) 127, 142, 344, 463, 588
BstMBI GATC 5 cut(s) 124, 139, 341, 460, 585
BstMCI CGRYCG 1 cut(s) 588
BstMWI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 401, 521
BstNI CCWGG 1 cut(s) 293
BstNSI RCATGY 1 cut(s) 682
BstSCI CCNGG 2 cut(s) 291, 574
BstV1I GCAGC 3 cut(s) 407, 511, 637
BstX2I RGATCY 2 cut(s) 341, 460
BstXI CCANNNNNNTGG 1 cut(s) 373
BstYI RGATCY 2 cut(s) 341, 460
BsuRI GGCC 2 cut(s) 591, 722
BtgI CCRYGG 1 cut(s) 390
BtsCI GGATG 3 cut(s) 239, 257, 383
BtsIMutI CAGTG 3 cut(s) 105, 282, 775
CaiI CAGNNNCTG 1 cut(s) 464
Cfr13I GGNCC 1 cut(s) 590
CviAII CATG 4 cut(s) 60, 251, 391, 679
DdeI CTNAG 1 cut(s) 516
DpnI GATC 5 cut(s) 126, 141, 343, 462, 587
DpnII GATC 5 cut(s) 124, 139, 341, 460, 585
Eco130I CCWWGG 2 cut(s) 337, 390
Eco32I GATATC 1 cut(s) 808
Eco57I CTGAAG 1 cut(s) 675
EcoRII CCWGG 1 cut(s) 291
EcoRV GATATC 1 cut(s) 808
EcoT14I CCWWGG 2 cut(s) 337, 390
ErhI CCWWGG 2 cut(s) 337, 390
FaeI CATG 4 cut(s) 63, 254, 394, 682
FaqI GGGAC 1 cut(s) 664
FatI CATG 4 cut(s) 59, 250, 390, 678
FbaI TGATCA 1 cut(s) 124
FblI GTMKAC 1 cut(s) 362
Fnu4HI GCNGC 4 cut(s) 7, 396, 525, 626
FokI GGATG 3 cut(s) 244, 246, 370
Fsp4HI GCNGC 4 cut(s) 7, 396, 525, 626
FspBI CTAG 1 cut(s) 741
GluI GCNGC 4 cut(s) 7, 396, 525, 626
HaeIII GGCC 2 cut(s) 591, 722
HapII CCGG 1 cut(s) 576
Hin1II CATG 4 cut(s) 63, 254, 394, 682
HindIII AAGCTT 2 cut(s) 71, 485
HinfI GANTC 3 cut(s) 48, 218, 446
HpaII CCGG 1 cut(s) 576
HphI GGTGA 2 cut(s) 79, 134
Hpy166II GTNNAC 2 cut(s) 363, 746
Hpy188I TCNGA 3 cut(s) 277, 460, 784
Hpy188III TCNNGA 3 cut(s) 45, 113, 830
Hpy8I GTNNAC 2 cut(s) 363, 746
Hpy99I CGWCG 1 cut(s) 446
HpyAV CCTTC 3 cut(s) 200, 313, 346
HpyCH4III ACNGT 3 cut(s) 97, 109, 779
HpyCH4IV ACGT 1 cut(s) 748
HpyCH4V TGCA 5 cut(s) 82, 404, 539, 625, 710
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 401, 521
HpyF3I CTNAG 1 cut(s) 516
HpySE526I ACGT 1 cut(s) 748
Hsp92II CATG 4 cut(s) 63, 254, 394, 682
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 5 cut(s) 124, 139, 341, 460, 585
LmnI GCTCC 2 cut(s) 38, 161
Lsp1109I GCAGC 3 cut(s) 407, 511, 637
LweI GCATC 4 cut(s) 50, 65, 224, 266
MaeI CTAG 1 cut(s) 741
MaeII ACGT 1 cut(s) 748
MaeIII GTNAC 2 cut(s) 31, 113
MalI GATC 5 cut(s) 126, 141, 343, 462, 587
MboI GATC 5 cut(s) 124, 139, 341, 460, 585
MboII GAAGA 4 cut(s) 215, 488, 668, 818
MflI RGATCY 2 cut(s) 341, 460
MluCI AATT 6 cut(s) 225, 299, 552, 561, 630, 659
MnlI CCTC 7 cut(s) 80, 290, 397, 429, 511, 603, 778
MroXI GAANNNNTTC 1 cut(s) 174
MseI TTAA 2 cut(s) 330, 822
MslI CAYNNNNRTG 1 cut(s) 620
MspI CCGG 1 cut(s) 576
MspR9I CCNGG 2 cut(s) 293, 576
MvaI CCWGG 1 cut(s) 293
MwoI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 401, 521
NciI CCSGG 1 cut(s) 576
NcoI CCATGG 1 cut(s) 390
NdeII GATC 5 cut(s) 124, 139, 341, 460, 585
NlaIII CATG 4 cut(s) 63, 254, 394, 682
NlaIV GGNNCC 2 cut(s) 343, 357
NmuCI GTSAC 2 cut(s) 31, 113
NspI RCATGY 1 cut(s) 682
OliI CACNNNNGTG 1 cut(s) 620
PdmI GAANNNNTTC 1 cut(s) 174
PfeI GAWTC 3 cut(s) 48, 218, 446
PfoI TCCNGGA 1 cut(s) 574
PkrI GCNGC 4 cut(s) 8, 397, 526, 627
Ple19I CGATCG 1 cut(s) 588
PsiI TTATAA 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 291
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 2 cut(s) 343, 357
PspPI GGNCC 1 cut(s) 590
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 2 cut(s) 341, 460
PvuI CGATCG 1 cut(s) 588
RseI CAYNNNNRTG 1 cut(s) 620
SaqAI TTAA 2 cut(s) 330, 822
SatI GCNGC 4 cut(s) 7, 396, 525, 626
Sau3AI GATC 5 cut(s) 124, 139, 341, 460, 585
Sau96I GGNCC 1 cut(s) 590
ScrFI CCNGG 2 cut(s) 293, 576
SfaNI GCATC 4 cut(s) 50, 65, 224, 266
SmiMI CAYNNNNRTG 1 cut(s) 620
Sse9I AATT 6 cut(s) 225, 299, 552, 561, 630, 659
SsiI CCGC 1 cut(s) 9
SspMI CTAG 1 cut(s) 741
StyD4I CCNGG 2 cut(s) 291, 574
StyI CCWWGG 2 cut(s) 337, 390
TaaI ACNGT 3 cut(s) 97, 109, 779
TaiI ACGT 1 cut(s) 751
TaqI TCGA 1 cut(s) 441
TasI AATT 6 cut(s) 225, 299, 552, 561, 630, 659
TfiI GAWTC 3 cut(s) 48, 218, 446
Tru1I TTAA 2 cut(s) 330, 822
Tru9I TTAA 2 cut(s) 330, 822
TscAI CASTG 3 cut(s) 112, 289, 782
TseFI GTSAC 2 cut(s) 31, 113
TseI GCWGC 4 cut(s) 6, 395, 524, 625
Tsp45I GTSAC 2 cut(s) 31, 113
TspDTI ATGAA 2 cut(s) 40, 167
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 3 cut(s) 112, 289, 782
XapI RAATTY 1 cut(s) 659
XceI RCATGY 1 cut(s) 682
XmiI GTMKAC 1 cut(s) 362
XmnI GAANNNNTTC 1 cut(s) 174
XspI CTAG 1 cut(s) 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.