Rroxscaffold_4G00330310

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000539
Physical Location & Seq
Forward (+)
63943066 .. 63945864
2799 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00330310.1

Sequence Viewer

Length: 906 bp
ATGAGCTGTGGAGAAAGCAAGGTTGTGTGTGTGACGGGAGCATCTGGTTTCATAGCATCATGGCTGGTGAAGCTCTTATTGCAACGAGATGATCAAAAGAAAACAGATCACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGTTCTTTTGACCCTGTTGTTGTTGGGTGTGAAGGTGTTTTTCATACAGCATCCCCTGTCCTACTCTCAGATTCATCTACTAATCCGCAGGCAGAATTAATTGACCCTGCTTTGAAGGGAACGCTTAATGTCCTTGGATCGTGTGTGAAGGTTCAGTCTATCAAAAGGGTGGTTATAACATCTTCTATAGTAGCAGTTGCATTTAATGGAAAACCTCTTACTGCTGATCTTTGGTATATCCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAGAAAGGACTTGATATTATTACAATAAATCCGGGATGGGTGATCGGCCCTCTTTTACAGCCAACTCTGAACCTTAGTGTTGAAATAGTTCTGAAACTCGTAAATGGGACGGAAACGTTTCCCAACAGAACTTACAGACTTGTTGATCTTAGAGATGTTGCTAATGCACATATTCTAGCCCTTGAAAACCCATCAGCTAGTGGACGTTATTGTTTAGTTGGAAGCGTAAAACACTGTTCAGAGGTCGTGAAAATGTTGTACGAGATTTCCCCTGCTCTCAATCTCCCAGATAATTTCTTACAAGGATACAAAATGCATGTTACTTCTGGCATAAATGTTCACCAGGTATCCAAGGAAAGAACCCAAACTTTGGGTGTAAAGTATACTCCGCTAGAAGTGTCTCTGAAGGATACTGTTGAAAGTTTGAAGAACAAGAACTTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

301

Amino Acids

32.9

Weight (kDa)

7.04

Isoelectric Point (pI)

31.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 9 - 220 3.8e-18 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 132 5.7e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 13 - 167 2.5e-06 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 350
AccB7I CCANNNNNTGG 1 cut(s) 829
AccI GTMKAC 1 cut(s) 842
AciI CCGC 2 cut(s) 260, 848
AclI AACGTT 1 cut(s) 575
AclWI GGATC 1 cut(s) 319
AcuI CTGAAG 1 cut(s) 884
AfaI GTAC 1 cut(s) 719
AfiI CCNNNNNNNGG 1 cut(s) 829
AgsI TTSAA 7 cut(s) 154, 289, 420, 542, 644, 878, 886
AjnI CCWGG 1 cut(s) 801
AluBI AGCT 5 cut(s) 6, 73, 431, 440, 656
AluI AGCT 5 cut(s) 6, 73, 431, 440, 656
Alw26I GTCTC 1 cut(s) 864
AlwI GGATC 1 cut(s) 319
AoxI GGCC 1 cut(s) 505
ApeKI GCWGC 1 cut(s) 440
AseI ATTAAT 1 cut(s) 272
Asp700I GAANNNNTTC 4 cut(s) 141, 546, 576, 899
AspS9I GGNCC 1 cut(s) 506
AsuC2I CCSGG 1 cut(s) 492
AsuHPI GGTGA 4 cut(s) 79, 101, 511, 791
BbvCI CCTCAGC 1 cut(s) 432
BbvI GCAGC 1 cut(s) 427
BccI CCATC 3 cut(s) 119, 489, 658
BciT130I CCWGG 1 cut(s) 803
BciVI GTATCC 3 cut(s) 758, 817, 862
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 1 cut(s) 492
BcoDI GTCTC 1 cut(s) 864
BfaI CTAG 3 cut(s) 635, 657, 851
BfmI CTRYAG 1 cut(s) 360
BfuI GTATCC 3 cut(s) 758, 817, 862
BisI GCNGC 1 cut(s) 441
BlsI GCNGC 1 cut(s) 442
Bme1390I CCNGG 2 cut(s) 492, 803
BmgT120I GGNCC 1 cut(s) 506
BmrFI CCNGG 2 cut(s) 492, 803
BmsI GCATC 3 cut(s) 50, 65, 233
Bpu10I CCTNAGC 1 cut(s) 432
BpuMI CCSGG 1 cut(s) 492
BsaJI CCNNGG 2 cut(s) 307, 810
Bsc4I CCNNNNNNNGG 1 cut(s) 829
BseBI CCWGG 1 cut(s) 803
BseDI CCNNGG 2 cut(s) 307, 810
BseGI GGATG 2 cut(s) 224, 500
BseLI CCNNNNNNNGG 1 cut(s) 829
BseMII CTCAG 2 cut(s) 255, 423
BseXI GCAGC 1 cut(s) 427
BshFI GGCC 1 cut(s) 507
BsiSI CCGG 1 cut(s) 491
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 1 cut(s) 829
BsmAI GTCTC 1 cut(s) 864
BsmFI GGGAC 1 cut(s) 580
BsnI GGCC 1 cut(s) 507
Bsp143I GATC 6 cut(s) 91, 106, 311, 400, 501, 604
BspACI CCGC 2 cut(s) 260, 848
BspANI GGCC 1 cut(s) 507
BspCNI CTCAG 2 cut(s) 254, 424
BspPI GGATC 1 cut(s) 319
BssECI CCNNGG 2 cut(s) 307, 810
BssMI GATC 6 cut(s) 91, 106, 311, 400, 501, 604
BssNAI GTATAC 1 cut(s) 843
BssT1I CCWWGG 2 cut(s) 307, 810
Bst1107I GTATAC 1 cut(s) 843
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 2 cut(s) 695, 874
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 4 cut(s) 241, 432, 533, 608
BstF5I GGATG 2 cut(s) 224, 500
BstKTI GATC 6 cut(s) 94, 109, 314, 403, 504, 607
BstMAI GTCTC 1 cut(s) 864
BstMBI GATC 6 cut(s) 91, 106, 311, 400, 501, 604
BstMWI GCNNNNNNNGC 3 cut(s) 70, 79, 437
BstNI CCWGG 1 cut(s) 803
BstNSI RCATGY 1 cut(s) 779
BstSCI CCNGG 2 cut(s) 490, 801
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 427
BstZ17I GTATAC 1 cut(s) 843
BsuI GTATCC 3 cut(s) 758, 817, 862
BsuRI GGCC 1 cut(s) 507
BtsCI GGATG 2 cut(s) 224, 500
BtsIMutI CAGTG 1 cut(s) 691
Cac8I GCNNGC 1 cut(s) 264
Cfr13I GGNCC 1 cut(s) 506
CsiI ACCWGGT 1 cut(s) 801
Csp6I GTAC 1 cut(s) 718
CviAII CATG 2 cut(s) 60, 776
CviQI GTAC 1 cut(s) 718
DdeI CTNAG 4 cut(s) 241, 432, 533, 608
DpnI GATC 6 cut(s) 93, 108, 313, 402, 503, 606
DpnII GATC 6 cut(s) 91, 106, 311, 400, 501, 604
Eco130I CCWWGG 2 cut(s) 307, 810
Eco57I CTGAAG 1 cut(s) 884
EcoRII CCWGG 1 cut(s) 801
EcoT14I CCWWGG 2 cut(s) 307, 810
EcoT22I ATGCAT 1 cut(s) 777
ErhI CCWWGG 2 cut(s) 307, 810
FaeI CATG 2 cut(s) 63, 779
FaqI GGGAC 1 cut(s) 580
FatI CATG 2 cut(s) 59, 775
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 842
Fnu4HI GCNGC 1 cut(s) 441
FokI GGATG 2 cut(s) 211, 507
Fsp4HI GCNGC 1 cut(s) 441
FspBI CTAG 3 cut(s) 635, 657, 851
GluI GCNGC 1 cut(s) 441
HaeIII GGCC 1 cut(s) 507
HapII CCGG 1 cut(s) 491
Hin1II CATG 2 cut(s) 63, 779
HinfI GANTC 1 cut(s) 245
HpaII CCGG 1 cut(s) 491
HphI GGTGA 4 cut(s) 79, 101, 511, 791
Hpy166II GTNNAC 3 cut(s) 662, 799, 843
Hpy188I TCNGA 5 cut(s) 244, 528, 552, 700, 864
Hpy188III TCNNGA 1 cut(s) 706
Hpy8I GTNNAC 3 cut(s) 662, 799, 843
HpyAV CCTTC 5 cut(s) 167, 200, 283, 316, 859
HpyCH4III ACNGT 2 cut(s) 695, 874
HpyCH4IV ACGT 2 cut(s) 575, 664
HpyCH4V TGCA 5 cut(s) 82, 374, 455, 626, 775
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 79, 437
HpyF3I CTNAG 4 cut(s) 241, 432, 533, 608
HpySE526I ACGT 2 cut(s) 575, 664
Hsp92II CATG 2 cut(s) 63, 779
Ksp22I TGATCA 1 cut(s) 91
Kzo9I GATC 6 cut(s) 91, 106, 311, 400, 501, 604
LmnI GCTCC 2 cut(s) 38, 128
Lsp1109I GCAGC 1 cut(s) 427
LweI GCATC 3 cut(s) 50, 65, 233
MabI ACCWGGT 1 cut(s) 801
MaeI CTAG 3 cut(s) 635, 657, 851
MaeII ACGT 2 cut(s) 575, 664
MaeIII GTNAC 2 cut(s) 31, 778
MalI GATC 6 cut(s) 93, 108, 313, 402, 503, 606
MboI GATC 6 cut(s) 91, 106, 311, 400, 501, 604
MboII GAAGA 3 cut(s) 182, 348, 898
MluCI AATT 3 cut(s) 269, 273, 751
MmeI TCCRAC 1 cut(s) 658
MnlI CCTC 4 cut(s) 399, 427, 519, 694
Mph1103I ATGCAT 1 cut(s) 777
MroXI GAANNNNTTC 4 cut(s) 141, 546, 576, 899
MseI TTAA 3 cut(s) 272, 300, 378
MspI CCGG 1 cut(s) 491
MspR9I CCNGG 2 cut(s) 492, 803
MvaI CCWGG 1 cut(s) 803
MwoI GCNNNNNNNGC 3 cut(s) 70, 79, 437
NciI CCSGG 1 cut(s) 492
NdeII GATC 6 cut(s) 91, 106, 311, 400, 501, 604
NlaIII CATG 2 cut(s) 63, 779
NmuCI GTSAC 1 cut(s) 31
NsiI ATGCAT 1 cut(s) 777
NspI RCATGY 1 cut(s) 779
PdmI GAANNNNTTC 4 cut(s) 141, 546, 576, 899
PfeI GAWTC 1 cut(s) 245
PflMI CCANNNNNTGG 1 cut(s) 829
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 1 cut(s) 442
PshBI ATTAAT 1 cut(s) 272
PsiI TTATAA 1 cut(s) 350
Psp1406I AACGTT 1 cut(s) 575
Psp6I CCWGG 1 cut(s) 801
PspGI CCWGG 1 cut(s) 801
PspPI GGNCC 1 cut(s) 506
RsaI GTAC 1 cut(s) 719
RsaNI GTAC 1 cut(s) 718
SaqAI TTAA 3 cut(s) 272, 300, 378
SatI GCNGC 1 cut(s) 441
Sau3AI GATC 6 cut(s) 91, 106, 311, 400, 501, 604
Sau96I GGNCC 1 cut(s) 506
ScrFI CCNGG 2 cut(s) 492, 803
SexAI ACCWGGT 1 cut(s) 801
SfaNI GCATC 3 cut(s) 50, 65, 233
SfcI CTRYAG 1 cut(s) 360
Sse9I AATT 3 cut(s) 269, 273, 751
SsiI CCGC 2 cut(s) 260, 848
SspMI CTAG 3 cut(s) 635, 657, 851
StyD4I CCNGG 2 cut(s) 490, 801
StyI CCWWGG 2 cut(s) 307, 810
TaaI ACNGT 2 cut(s) 695, 874
TaiI ACGT 2 cut(s) 578, 667
TasI AATT 3 cut(s) 269, 273, 751
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 3 cut(s) 272, 300, 378
Tru9I TTAA 3 cut(s) 272, 300, 378
TscAI CASTG 1 cut(s) 698
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 1 cut(s) 440
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 40, 134, 206, 237
TspGWI ACGGA 1 cut(s) 584
TspRI CASTG 1 cut(s) 698
Van91I CCANNNNNTGG 1 cut(s) 829
VspI ATTAAT 1 cut(s) 272
XceI RCATGY 1 cut(s) 779
XmiI GTMKAC 1 cut(s) 842
XmnI GAANNNNTTC 4 cut(s) 141, 546, 576, 899
XspI CTAG 3 cut(s) 635, 657, 851
Zsp2I ATGCAT 1 cut(s) 777
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.