Rroxscaffold_4G00330240

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000539
Physical Location & Seq
Forward (+)
63725742 .. 63728329
2588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00330240.1

Sequence Viewer

Length: 972 bp
ATGAGCTGCGGAGAAAGCAAGGTTGTGTGTGTGACAGGAGCATCTGGGTTCATAGCATCATGGCTAGTTAAGCTCTTATTGCAACGAGGTTATACTGTTAAAGCCAGTGTTCGGGACCTAAATGATCAAAAGAAAACAGAGCACCTACTCTCATTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGTTCTTTTGACCCTGTCGTTGATGGGTGTGAAGGTGTTTTTCATACAGCATCCCCTGTCGTATTCTCATCTACTAATCCGCAGGCAGAATTAATTGAGCCTGCTTTGAAGGGAACGCTTAATGTCCTTGGATCGTGTGTGAAGGTCCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATGGCAGCAGTTAGATTTAATGGAAAACCTCTTACTGCTGATGTAATAATCGATGAATCTTGGTTTTCAGATCCTGCTTTTATTTGTTTTCATCAGCTTTGGTATATGCTTTCAAAGACGTTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAGAAAGGAATTGATATTATTACAATAAATCCCGGATGGGTGATCGGCCCTCCCTTACAGCCAACTCTGAACTTAACTGTGGAACTAGTTCTGAAACTCGTAAATGGGACCGAAAAGTTTCCCAACGAAACTTACAGATTTGTTGATGTTAGAGATGTTGCTAATGCACATATTCTAGCCTTTGAAAACCCATCTGCTAGTGGACGTTATTGTTTAGTTGGAAGCGTAAAACACTGTTCAGAGGTTGTGAAAATGTTGCACGAGATCTTCCCTGCTCTCAATCTTCCAGATAAGTGTGCAGATGACAAGCCTTTCACACCAACTTACCAGGTATCCAAGGAAAGAGCCCAAACTTTGGGTGTAAAATATACTCCGCTTGAAGTGTCTCTGAAGGATACTGTTGAAAGTTTGAAGGACAAGAACTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

323

Amino Acids

35.71

Weight (kDa)

6.19

Isoelectric Point (pI)

27.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 99 1.2e-08 NmrA-like family
Epimerase PF01370 9 - 247 1.6e-22 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 242 6.4e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 11 - 200 2.4e-12 Male sterility protein
GDP_Man_Dehyd PF16363 11 - 130 4.7e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 134 1.7e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 377
AccB7I CCANNNNNTGG 1 cut(s) 895
AciI CCGC 3 cut(s) 9, 287, 914
AclWI GGATC 2 cut(s) 346, 452
AcuI CTGAAG 1 cut(s) 950
AfiI CCNNNNNNNGG 2 cut(s) 111, 895
AgsI TTSAA 7 cut(s) 187, 316, 501, 725, 920, 944, 952
AhlI ACTAGT 1 cut(s) 625
AjnI CCWGG 1 cut(s) 867
AluBI AGCT 5 cut(s) 6, 73, 484, 512, 521
AluI AGCT 5 cut(s) 6, 73, 484, 512, 521
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 1 cut(s) 930
AlwI GGATC 2 cut(s) 346, 452
AlwNI CAGNNNCTG 1 cut(s) 461
AoxI GGCC 1 cut(s) 586
ApeKI GCWGC 3 cut(s) 6, 392, 521
AseI ATTAAT 1 cut(s) 299
Asp700I GAANNNNTTC 4 cut(s) 174, 627, 657, 965
AspS9I GGNCC 4 cut(s) 115, 352, 587, 648
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 1 cut(s) 592
AvaII GGWCC 3 cut(s) 115, 352, 648
BanII GRGCYC 1 cut(s) 889
BauI CACGAG 1 cut(s) 800
Bbv12I GWGCWC 1 cut(s) 144
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 2 cut(s) 404, 508
BccI CCATC 4 cut(s) 152, 224, 570, 739
BciT130I CCWGG 1 cut(s) 869
BciVI GTATCC 2 cut(s) 883, 928
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 1 cut(s) 573
BcoDI GTCTC 1 cut(s) 930
BcuI ACTAGT 1 cut(s) 625
BfaI CTAG 4 cut(s) 65, 626, 716, 738
BfuI GTATCC 2 cut(s) 883, 928
BglII AGATCT 1 cut(s) 804
BisI GCNGC 3 cut(s) 7, 393, 522
BlsI GCNGC 3 cut(s) 8, 394, 523
Bme1390I CCNGG 2 cut(s) 573, 869
Bme18I GGWCC 3 cut(s) 115, 352, 648
BmgT120I GGNCC 4 cut(s) 115, 352, 587, 648
BmiI GGNNCC 2 cut(s) 116, 649
BmrFI CCNGG 2 cut(s) 573, 869
BmsI GCATC 3 cut(s) 50, 65, 266
Bpu10I CCTNAGC 1 cut(s) 513
BpuMI CCSGG 1 cut(s) 573
Bsa29I ATCGAT 1 cut(s) 438
BsaJI CCNNGG 2 cut(s) 334, 876
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 895
Bse1I ACTGG 2 cut(s) 105, 355
BseBI CCWGG 1 cut(s) 869
BseCI ATCGAT 1 cut(s) 438
BseDI CCNNGG 2 cut(s) 334, 876
BseGI GGATG 3 cut(s) 257, 380, 581
BseLI CCNNNNNNNGG 2 cut(s) 111, 895
BseMII CTCAG 1 cut(s) 504
BseNI ACTGG 2 cut(s) 105, 355
BseXI GCAGC 2 cut(s) 404, 508
BsgI GTGCAG 1 cut(s) 858
BshFI GGCC 1 cut(s) 588
BshVI ATCGAT 1 cut(s) 438
BsiHKAI GWGCWC 1 cut(s) 144
BsiSI CCGG 1 cut(s) 573
BslFI GGGAC 2 cut(s) 128, 661
BslI CCNNNNNNNGG 2 cut(s) 111, 895
BsmAI GTCTC 1 cut(s) 930
BsmFI GGGAC 2 cut(s) 128, 661
BsnI GGCC 1 cut(s) 588
Bsp1286I GDGCHC 2 cut(s) 144, 889
Bsp143I GATC 5 cut(s) 124, 338, 457, 582, 804
BspACI CCGC 3 cut(s) 9, 287, 914
BspANI GGCC 1 cut(s) 588
BspCNI CTCAG 1 cut(s) 505
BspDI ATCGAT 1 cut(s) 438
BspLI GGNNCC 2 cut(s) 116, 649
BspPI GGATC 2 cut(s) 346, 452
BsrI ACTGG 2 cut(s) 105, 355
BssECI CCNNGG 2 cut(s) 334, 876
BssMI GATC 5 cut(s) 124, 338, 457, 582, 804
BssSI CACGAG 1 cut(s) 800
BssT1I CCWWGG 2 cut(s) 334, 876
Bst2BI CACGAG 1 cut(s) 800
Bst2UI CCWGG 1 cut(s) 869
Bst4CI ACNGT 4 cut(s) 97, 619, 776, 940
BstC8I GCNNGC 2 cut(s) 291, 309
BstDEI CTNAG 1 cut(s) 513
BstF5I GGATG 3 cut(s) 257, 380, 581
BstKTI GATC 5 cut(s) 127, 341, 460, 585, 807
BstMAI GTCTC 1 cut(s) 930
BstMBI GATC 5 cut(s) 124, 338, 457, 582, 804
BstMWI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 518
BstNI CCWGG 1 cut(s) 869
BstSCI CCNGG 2 cut(s) 571, 867
BstV1I GCAGC 2 cut(s) 404, 508
BstX2I RGATCY 2 cut(s) 457, 804
BstYI RGATCY 2 cut(s) 457, 804
Bsu15I ATCGAT 1 cut(s) 438
BsuI GTATCC 2 cut(s) 883, 928
BsuRI GGCC 1 cut(s) 588
BsuTUI ATCGAT 1 cut(s) 438
BtsCI GGATG 3 cut(s) 257, 380, 581
BtsIMutI CAGTG 2 cut(s) 112, 772
Cac8I GCNNGC 2 cut(s) 291, 309
CaiI CAGNNNCTG 1 cut(s) 461
Cfr13I GGNCC 4 cut(s) 115, 352, 587, 648
ClaI ATCGAT 1 cut(s) 438
CsiI ACCWGGT 1 cut(s) 867
CviAII CATG 1 cut(s) 60
DdeI CTNAG 1 cut(s) 513
DpnI GATC 5 cut(s) 126, 340, 459, 584, 806
DpnII GATC 5 cut(s) 124, 338, 457, 582, 804
Eco130I CCWWGG 2 cut(s) 334, 876
Eco24I GRGCYC 1 cut(s) 889
Eco47I GGWCC 3 cut(s) 115, 352, 648
Eco57I CTGAAG 1 cut(s) 950
EcoO109I RGGNCCY 1 cut(s) 115
EcoRII CCWGG 1 cut(s) 867
EcoT14I CCWWGG 2 cut(s) 334, 876
EcoT38I GRGCYC 1 cut(s) 889
ErhI CCWWGG 2 cut(s) 334, 876
FaeI CATG 1 cut(s) 63
FalI AAGNNNNNCTT 1 cut(s) 950
FaqI GGGAC 2 cut(s) 128, 661
FatI CATG 1 cut(s) 59
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 3 cut(s) 7, 393, 522
FokI GGATG 3 cut(s) 244, 367, 588
FriOI GRGCYC 1 cut(s) 889
Fsp4HI GCNGC 3 cut(s) 7, 393, 522
FspBI CTAG 4 cut(s) 65, 626, 716, 738
GluI GCNGC 3 cut(s) 7, 393, 522
HaeIII GGCC 1 cut(s) 588
HapII CCGG 1 cut(s) 573
Hin1II CATG 1 cut(s) 63
HinfI GANTC 1 cut(s) 443
HpaII CCGG 1 cut(s) 573
HphI GGTGA 1 cut(s) 592
Hpy166II GTNNAC 1 cut(s) 743
Hpy188I TCNGA 5 cut(s) 457, 609, 633, 781, 930
Hpy188III TCNNGA 2 cut(s) 113, 827
Hpy8I GTNNAC 1 cut(s) 743
HpyAV CCTTC 6 cut(s) 200, 233, 310, 343, 925, 946
HpyCH4III ACNGT 4 cut(s) 97, 619, 776, 940
HpyCH4IV ACGT 2 cut(s) 506, 745
HpyCH4V TGCA 5 cut(s) 82, 536, 707, 799, 839
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 518
HpyF3I CTNAG 1 cut(s) 513
HpySE526I ACGT 2 cut(s) 506, 745
Hsp92II CATG 1 cut(s) 63
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 5 cut(s) 124, 338, 457, 582, 804
LmnI GCTCC 2 cut(s) 38, 161
Lsp1109I GCAGC 2 cut(s) 404, 508
LweI GCATC 3 cut(s) 50, 65, 266
MabI ACCWGGT 1 cut(s) 867
MaeI CTAG 4 cut(s) 65, 626, 716, 738
MaeII ACGT 2 cut(s) 506, 745
MaeIII GTNAC 1 cut(s) 31
MalI GATC 5 cut(s) 126, 340, 459, 584, 806
MboI GATC 5 cut(s) 124, 338, 457, 582, 804
MboII GAAGA 4 cut(s) 215, 799, 815, 958
MflI RGATCY 2 cut(s) 457, 804
MhlI GDGCHC 2 cut(s) 144, 889
MluCI AATT 3 cut(s) 296, 300, 549
MmeI TCCRAC 1 cut(s) 739
MnlI CCTC 6 cut(s) 80, 394, 426, 508, 600, 775
MroXI GAANNNNTTC 4 cut(s) 174, 627, 657, 965
MseI TTAA 6 cut(s) 69, 99, 299, 327, 405, 614
MspI CCGG 1 cut(s) 573
MspR9I CCNGG 2 cut(s) 573, 869
MvaI CCWGG 1 cut(s) 869
MwoI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 518
NciI CCSGG 1 cut(s) 573
NdeII GATC 5 cut(s) 124, 338, 457, 582, 804
NlaIII CATG 1 cut(s) 63
NlaIV GGNNCC 2 cut(s) 116, 649
NmuCI GTSAC 1 cut(s) 31
PdmI GAANNNNTTC 4 cut(s) 174, 627, 657, 965
PfeI GAWTC 1 cut(s) 443
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 895
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 3 cut(s) 8, 394, 523
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 299
PsiI TTATAA 1 cut(s) 377
Psp5II RGGWCCY 1 cut(s) 115
Psp6I CCWGG 1 cut(s) 867
PspGI CCWGG 1 cut(s) 867
PspN4I GGNNCC 2 cut(s) 116, 649
PspPI GGNCC 4 cut(s) 115, 352, 587, 648
PspPPI RGGWCCY 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 2 cut(s) 457, 804
PsyI GACNNNGTC 1 cut(s) 221
SaqAI TTAA 6 cut(s) 69, 99, 299, 327, 405, 614
SatI GCNGC 3 cut(s) 7, 393, 522
Sau3AI GATC 5 cut(s) 124, 338, 457, 582, 804
Sau96I GGNCC 4 cut(s) 115, 352, 587, 648
ScrFI CCNGG 2 cut(s) 573, 869
SduI GDGCHC 2 cut(s) 144, 889
SexAI ACCWGGT 1 cut(s) 867
SfaNI GCATC 3 cut(s) 50, 65, 266
SinI GGWCC 3 cut(s) 115, 352, 648
SpeI ACTAGT 1 cut(s) 625
Sse9I AATT 3 cut(s) 296, 300, 549
SsiI CCGC 3 cut(s) 9, 287, 914
SspMI CTAG 4 cut(s) 65, 626, 716, 738
StyD4I CCNGG 2 cut(s) 571, 867
StyI CCWWGG 2 cut(s) 334, 876
TaaI ACNGT 4 cut(s) 97, 619, 776, 940
TaiI ACGT 2 cut(s) 509, 748
TaqI TCGA 1 cut(s) 438
TaqII GACCGA 1 cut(s) 665
TasI AATT 3 cut(s) 296, 300, 549
TfiI GAWTC 1 cut(s) 443
Tru1I TTAA 6 cut(s) 69, 99, 299, 327, 405, 614
Tru9I TTAA 6 cut(s) 69, 99, 299, 327, 405, 614
TscAI CASTG 2 cut(s) 112, 779
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 3 cut(s) 6, 392, 521
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 5 cut(s) 40, 167, 239, 456, 467
TspRI CASTG 2 cut(s) 112, 779
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 895
VpaK11BI GGWCC 3 cut(s) 115, 352, 648
VspI ATTAAT 1 cut(s) 299
XmnI GAANNNNTTC 4 cut(s) 174, 627, 657, 965
XspI CTAG 4 cut(s) 65, 626, 716, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.