pycom02g25510

reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000539
Physical Location & Seq
Forward (+)
23394673 .. 23397776
3104 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g25510.2

Sequence Viewer

Length: 975 bp
ATGAGTGACACCAGAGCAACAAAGGTGGTGAGTGTAACCGGAGCGTCCGGTTACATAGCATCATGGCTGGTGAAGCTGTTGCTGCAGCGTGGGTATACGGTCAAAGCCTCAGTTCGCGACCCAAATCATAAGAAGAAAACAGCACACTTGCTTGCGCTAGATGGAGCTAAGGAGAGGCTTCAACTATTCAAAGCAGACCTACTGGAAGAGGGCTCTTTTGATTCCCTAGTTGAGGGTAGTGAAGGAGTTTTTCATACAGCTTCTCCATTTTATCACAATGTCAGCGACCCACAGGCGGAACTTATTGACCCTGCTTTGAAGGGAACTCTTAATGTCCTTAGATCGTGCGCCAAGGTTCCGTCTATCAAAAGGGTGGTAATAACGTCCTCCATGGCAGCAGTTGCATTTAATGGAAAACCTCTTGCTCCTGATGTAGTAGTTGATGAATCTTGGTTTTCAGATGTAGCTGTTTGTGAAAAATCAAAGCTTTGGTATATGATTTCAAAGACGTTGGCTGAGGAAGCTGCTTGGAAGTTTGCGAAAGAGAATGGAATCGACATCGTTACAATAAATCCAGGATTGGTGACTGGTCCTCTCTTACAGCCAACTCTGAACACAAGCGTGGAACCTGTTCTGAAACTCGTCAATGGAGCTGAAAGATTTCCAAATACAACTTATAGATGGGTTGATGTTAGAGATGTTGCTACTGCTCATATTCTCGCCCTTGAGAATGCTTCAGCAAGTGGGAGATATTGTCTAGTTGGAAGAGTAATACACTGTTCTGACACCGTGAATATTCTGCGGGATCTCTTCCCTGCTCTCAAGCTTCCAGAGGAATGTGCAGATGACAAACCATTGACACCAACTTACCAGGTATCCAATGAAAGAGCCCAAAGTTTGGGAGTTAAGTTTACTCCTCTGGAGGTGACTTTGAAGGATACCGTGGAAAGTTTGAAGGAGAAGAACTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

325

Amino Acids

35.5

Weight (kDa)

6.61

Isoelectric Point (pI)

32.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 898
AccI GTMKAC 1 cut(s) 95
AccII CGCG 1 cut(s) 117
AciI CCGC 2 cut(s) 296, 802
AclWI GGATC 1 cut(s) 813
AcuI CTGAAG 1 cut(s) 720
AfiI CCNNNNNNNGG 3 cut(s) 232, 295, 898
AgsI TTSAA 6 cut(s) 182, 190, 319, 504, 934, 955
AjnI CCWGG 2 cut(s) 574, 870
AleI CACNNNNGTG 1 cut(s) 620
AluBI AGCT 8 cut(s) 76, 167, 260, 467, 487, 524, 653, 826
AluI AGCT 8 cut(s) 76, 167, 260, 467, 487, 524, 653, 826
AlwI GGATC 1 cut(s) 813
ApeKI GCWGC 4 cut(s) 82, 85, 395, 524
Asp700I GAANNNNTTC 4 cut(s) 630, 660, 965, 968
AspLEI GCGC 2 cut(s) 157, 350
AspS9I GGNCC 1 cut(s) 590
AsuHPI GGTGA 4 cut(s) 40, 82, 595, 937
AvaII GGWCC 1 cut(s) 590
BanII GRGCYC 2 cut(s) 215, 892
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 4 cut(s) 69, 97, 407, 511
BccI CCATC 2 cut(s) 155, 675
BciT130I CCWGG 2 cut(s) 576, 872
BciVI GTATCC 2 cut(s) 886, 931
BfaI CTAG 3 cut(s) 158, 227, 758
BfmI CTRYAG 1 cut(s) 83
BfuI GTATCC 2 cut(s) 886, 931
BisI GCNGC 4 cut(s) 83, 86, 396, 525
BlsI GCNGC 4 cut(s) 84, 87, 397, 526
Bme1390I CCNGG 2 cut(s) 576, 872
Bme18I GGWCC 1 cut(s) 590
BmgT120I GGNCC 1 cut(s) 590
BmiI GGNNCC 2 cut(s) 357, 627
BmrFI CCNGG 2 cut(s) 576, 872
BmsI GCATC 1 cut(s) 68
BpmI CTGGAG 1 cut(s) 941
Bpu10I CCTNAGC 2 cut(s) 168, 516
BpuEI CTTGAG 2 cut(s) 746, 806
BsaJI CCNNGG 3 cut(s) 351, 390, 942
BsaWI WCCGGW 2 cut(s) 38, 47
BsaXI ACNNNNNCTCC 4 cut(s) 247, 277, 739, 769
Bsc4I CCNNNNNNNGG 3 cut(s) 232, 295, 898
Bse1I ACTGG 2 cut(s) 207, 592
BseBI CCWGG 2 cut(s) 576, 872
BseDI CCNNGG 3 cut(s) 351, 390, 942
BseLI CCNNNNNNNGG 3 cut(s) 232, 295, 898
BseMII CTCAG 2 cut(s) 123, 507
BseNI ACTGG 2 cut(s) 207, 592
BseRI GAGGAG 1 cut(s) 906
BseXI GCAGC 4 cut(s) 69, 97, 407, 511
BsgI GTGCAG 1 cut(s) 861
Bsh1236I CGCG 1 cut(s) 117
BsiSI CCGG 2 cut(s) 39, 48
BslI CCNNNNNNNGG 3 cut(s) 232, 295, 898
BsmI GAATGC 1 cut(s) 736
Bsp1286I GDGCHC 2 cut(s) 215, 892
Bsp143I GATC 2 cut(s) 341, 805
Bsp19I CCATGG 1 cut(s) 390
Bsp68I TCGCGA 1 cut(s) 117
BspACI CCGC 2 cut(s) 296, 802
BspCNI CTCAG 2 cut(s) 122, 508
BspFNI CGCG 1 cut(s) 117
BspLI GGNNCC 2 cut(s) 357, 627
BspMAI CTGCAG 1 cut(s) 87
BspPI GGATC 1 cut(s) 813
BsrI ACTGG 2 cut(s) 207, 592
BssECI CCNNGG 3 cut(s) 351, 390, 942
BssMI GATC 2 cut(s) 341, 805
BssNAI GTATAC 1 cut(s) 96
BssT1I CCWWGG 2 cut(s) 351, 390
Bst1107I GTATAC 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 576, 872
Bst4CI ACNGT 4 cut(s) 100, 779, 790, 943
Bst6I CTCTTC 3 cut(s) 201, 760, 815
BstAPI GCANNNNNTGC 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 153
BstDEI CTNAG 4 cut(s) 109, 168, 338, 516
BstDSI CCRYGG 2 cut(s) 390, 942
BstENI CCTNNNNNAGG 1 cut(s) 230
BstFNI CGCG 1 cut(s) 117
BstHHI GCGC 2 cut(s) 157, 350
BstKTI GATC 2 cut(s) 344, 808
BstMBI GATC 2 cut(s) 341, 805
BstMWI GCNNNNNNNGC 4 cut(s) 73, 82, 401, 521
BstNI CCWGG 2 cut(s) 576, 872
BstSCI CCNGG 2 cut(s) 574, 870
BstSFI CTRYAG 1 cut(s) 83
BstUI CGCG 1 cut(s) 117
BstV1I GCAGC 4 cut(s) 69, 97, 407, 511
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
BstZ17I GTATAC 1 cut(s) 96
BsuI GTATCC 2 cut(s) 886, 931
BtgI CCRYGG 2 cut(s) 390, 942
BtsIMutI CAGTG 1 cut(s) 775
BtuMI TCGCGA 1 cut(s) 117
Cac8I GCNNGC 1 cut(s) 153
CfoI GCGC 2 cut(s) 157, 350
Cfr13I GGNCC 1 cut(s) 590
CseI GACGC 1 cut(s) 33
CsiI ACCWGGT 1 cut(s) 870
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 2 cut(s) 63, 391
DdeI CTNAG 4 cut(s) 109, 168, 338, 516
DpnI GATC 2 cut(s) 343, 807
DpnII GATC 2 cut(s) 341, 805
Eam1104I CTCTTC 3 cut(s) 201, 760, 815
EarI CTCTTC 3 cut(s) 201, 760, 815
EciI GGCGGA 1 cut(s) 311
Eco130I CCWWGG 2 cut(s) 351, 390
Eco24I GRGCYC 2 cut(s) 215, 892
Eco47I GGWCC 1 cut(s) 590
Eco57I CTGAAG 1 cut(s) 720
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoRII CCWGG 2 cut(s) 574, 870
EcoT14I CCWWGG 2 cut(s) 351, 390
EcoT38I GRGCYC 2 cut(s) 215, 892
ErhI CCWWGG 2 cut(s) 351, 390
FaeI CATG 2 cut(s) 66, 394
FalI AAGNNNNNCTT 1 cut(s) 953
FatI CATG 2 cut(s) 62, 390
FauI CCCGC 1 cut(s) 795
FblI GTMKAC 1 cut(s) 95
Fnu4HI GCNGC 4 cut(s) 83, 86, 396, 525
FriOI GRGCYC 2 cut(s) 215, 892
Fsp4HI GCNGC 4 cut(s) 83, 86, 396, 525
FspBI CTAG 3 cut(s) 158, 227, 758
GlaI GCGC 2 cut(s) 156, 349
GluI GCNGC 4 cut(s) 83, 86, 396, 525
GsuI CTGGAG 1 cut(s) 941
HapII CCGG 2 cut(s) 39, 48
HgaI GACGC 1 cut(s) 33
HhaI GCGC 2 cut(s) 157, 350
Hin1II CATG 2 cut(s) 66, 394
Hin6I GCGC 2 cut(s) 155, 348
HinP1I GCGC 2 cut(s) 155, 348
HindIII AAGCTT 2 cut(s) 485, 824
HinfI GANTC 3 cut(s) 221, 446, 552
HpaII CCGG 2 cut(s) 39, 48
HphI GGTGA 4 cut(s) 40, 82, 595, 937
Hpy166II GTNNAC 2 cut(s) 96, 912
Hpy188I TCNGA 5 cut(s) 460, 612, 636, 784, 974
Hpy188III TCNNGA 4 cut(s) 116, 428, 830, 920
Hpy8I GTNNAC 2 cut(s) 96, 912
HpyAV CCTTC 4 cut(s) 236, 313, 928, 949
HpyCH4III ACNGT 4 cut(s) 100, 779, 790, 943
HpyCH4IV ACGT 2 cut(s) 383, 509
HpyCH4V TGCA 3 cut(s) 85, 404, 842
HpyF10VI GCNNNNNNNGC 4 cut(s) 73, 82, 401, 521
HpyF3I CTNAG 4 cut(s) 109, 168, 338, 516
HpySE526I ACGT 2 cut(s) 383, 509
Hsp92II CATG 2 cut(s) 66, 394
HspAI GCGC 2 cut(s) 155, 348
Kzo9I GATC 2 cut(s) 341, 805
LmnI GCTCC 4 cut(s) 41, 164, 430, 650
Lsp1109I GCAGC 4 cut(s) 69, 97, 407, 511
LweI GCATC 1 cut(s) 68
MabI ACCWGGT 1 cut(s) 870
MaeI CTAG 3 cut(s) 158, 227, 758
MaeII ACGT 2 cut(s) 383, 509
MaeIII GTNAC 6 cut(s) 5, 34, 50, 562, 583, 925
MalI GATC 2 cut(s) 343, 807
MboI GATC 2 cut(s) 341, 805
MboII GAAGA 6 cut(s) 145, 218, 777, 802, 961, 973
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 2 cut(s) 215, 892
MmeI TCCRAC 1 cut(s) 742
MroXI GAANNNNTTC 4 cut(s) 630, 660, 965, 968
MseI TTAA 3 cut(s) 330, 408, 906
MslI CAYNNNNRTG 1 cut(s) 620
MspI CCGG 2 cut(s) 39, 48
MspR9I CCNGG 2 cut(s) 576, 872
Mva1269I GAATGC 1 cut(s) 736
MvaI CCWGG 2 cut(s) 576, 872
MvnI CGCG 1 cut(s) 117
MwoI GCNNNNNNNGC 4 cut(s) 73, 82, 401, 521
NcoI CCATGG 1 cut(s) 390
NdeII GATC 2 cut(s) 341, 805
NlaIII CATG 2 cut(s) 66, 394
NlaIV GGNNCC 2 cut(s) 357, 627
NmuCI GTSAC 3 cut(s) 5, 583, 925
NruI TCGCGA 1 cut(s) 117
OliI CACNNNNGTG 1 cut(s) 620
PctI GAATGC 1 cut(s) 736
PdmI GAANNNNTTC 4 cut(s) 630, 660, 965, 968
PfeI GAWTC 3 cut(s) 221, 446, 552
PflMI CCANNNNNTGG 1 cut(s) 898
PfoI TCCNGGA 1 cut(s) 574
PkrI GCNGC 4 cut(s) 84, 87, 397, 526
Psp6I CCWGG 2 cut(s) 574, 870
PspGI CCWGG 2 cut(s) 574, 870
PspN4I GGNNCC 2 cut(s) 357, 627
PspPI GGNCC 1 cut(s) 590
PstI CTGCAG 1 cut(s) 87
PsuI RGATCY 1 cut(s) 805
RruI TCGCGA 1 cut(s) 117
RseI CAYNNNNRTG 1 cut(s) 620
SaqAI TTAA 3 cut(s) 330, 408, 906
SatI GCNGC 4 cut(s) 83, 86, 396, 525
Sau3AI GATC 2 cut(s) 341, 805
Sau96I GGNCC 1 cut(s) 590
ScrFI CCNGG 2 cut(s) 576, 872
SduI GDGCHC 2 cut(s) 215, 892
SexAI ACCWGGT 1 cut(s) 870
SfaNI GCATC 1 cut(s) 68
SfcI CTRYAG 1 cut(s) 83
SinI GGWCC 1 cut(s) 590
SmiMI CAYNNNNRTG 1 cut(s) 620
SmlI CTYRAG 2 cut(s) 725, 821
SmoI CTYRAG 2 cut(s) 725, 821
SsiI CCGC 2 cut(s) 296, 802
SspI AATATT 1 cut(s) 796
SspMI CTAG 3 cut(s) 158, 227, 758
StyD4I CCNGG 2 cut(s) 574, 870
StyI CCWWGG 2 cut(s) 351, 390
TaaI ACNGT 4 cut(s) 100, 779, 790, 943
TaiI ACGT 2 cut(s) 386, 512
TaqI TCGA 1 cut(s) 555
TfiI GAWTC 3 cut(s) 221, 446, 552
Tru1I TTAA 3 cut(s) 330, 408, 906
Tru9I TTAA 3 cut(s) 330, 408, 906
TscAI CASTG 1 cut(s) 782
TseFI GTSAC 3 cut(s) 5, 583, 925
TseI GCWGC 4 cut(s) 82, 85, 395, 524
Tsp45I GTSAC 3 cut(s) 5, 583, 925
TspDTI ATGAA 3 cut(s) 242, 459, 897
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 1 cut(s) 782
Van91I CCANNNNNTGG 1 cut(s) 898
VpaK11BI GGWCC 1 cut(s) 590
XagI CCTNNNNNAGG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 95
XmnI GAANNNNTTC 4 cut(s) 630, 660, 965, 968
XspI CTAG 3 cut(s) 158, 227, 758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.