RLG00000030526

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000539
Physical Location & Seq
Reverse (-)
62943443 .. 62945375
1933 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030526

Sequence Viewer

Length: 708 bp
ATGTTGCTAGCTCTTAATCGTTGTCAGGCAGAATTAATTGACCCTGCTTTGAAGGGAACGCTTAATGTCCTTGGATCGTGTGTGAAGGTTCAGTCTATCAAAAGGGTGGTTATAACATCTTCTATAGTAGCAGTTGCATTTAATGGAAAACCTCTTACTGCTGATGTAATAATTGATGAATCTTGGTTTTCAGATCCTGCTTTTTGTGAAAAAGCGAAGCTTTGGTATATCCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAGAAAGGAATTGATATTATTACAATAAATCCGGGATGGGTGATCGGCCCTCTTTTACAGCCAACTCTGAACCTTAGTGTTGAAATAGTTCTGAAACTCGTAAATGGGACCGAAACGTTTCCCAACAGAACTTACAGACTTGTTGATGTTAGAGATGTTGCTAATGCACATATTCTAGCGCTTGAAAACCCATCAGCTAGTGGACGTTATTGTTTAGTTGGAAGCGTAAAACACTGTTCAGAGGTCGTGAAAATGTTGTACGAGATTTCCCCTGCTCTCAATCTCCCAGACAAATGTGCAGACGACAAGCCTTTCACACCAACTCACCAGGTATCCAAGGAAAGAACCCAAACTTTGGGTGTAAAGTATACTCCGCTGGAAGTGTCTCTGAAGGATACTGTTGAAAGTTTGAAGAACAAGAACTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

236

Amino Acids

25.96

Weight (kDa)

8.15

Isoelectric Point (pI)

24.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 11 - 159 8.6e-09 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 14 - 155 2.5e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 113
AccB7I CCANNNNNTGG 1 cut(s) 631
AccI GTMKAC 1 cut(s) 644
AciI CCGC 1 cut(s) 650
AclI AACGTT 1 cut(s) 392
AclWI GGATC 2 cut(s) 82, 188
AcuI CTGAAG 1 cut(s) 686
AfaI GTAC 1 cut(s) 536
AfeI AGCGCT 1 cut(s) 456
AfiI CCNNNNNNNGG 1 cut(s) 631
AgsI TTSAA 6 cut(s) 52, 237, 359, 461, 680, 688
AjnI CCWGG 1 cut(s) 603
AluBI AGCT 5 cut(s) 11, 220, 248, 257, 473
AluI AGCT 5 cut(s) 11, 220, 248, 257, 473
Alw26I GTCTC 1 cut(s) 666
AlwI GGATC 2 cut(s) 82, 188
AlwNI CAGNNNCTG 1 cut(s) 197
Aor51HI AGCGCT 1 cut(s) 456
AoxI GGCC 1 cut(s) 322
ApeKI GCWGC 1 cut(s) 257
AseI ATTAAT 1 cut(s) 35
Asp700I GAANNNNTTC 3 cut(s) 363, 393, 701
AspLEI GCGC 1 cut(s) 457
AspS9I GGNCC 2 cut(s) 323, 384
AsuC2I CCSGG 1 cut(s) 309
AsuHPI GGTGA 2 cut(s) 328, 593
AsuNHI GCTAGC 1 cut(s) 7
AvaII GGWCC 1 cut(s) 384
BbvCI CCTCAGC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 244
BccI CCATC 2 cut(s) 306, 475
BciT130I CCWGG 1 cut(s) 605
BciVI GTATCC 2 cut(s) 619, 664
BcnI CCSGG 1 cut(s) 309
BcoDI GTCTC 1 cut(s) 666
BfaI CTAG 3 cut(s) 8, 452, 474
BfmI CTRYAG 1 cut(s) 123
BfoI RGCGCY 1 cut(s) 458
BfuI GTATCC 2 cut(s) 619, 664
BisI GCNGC 1 cut(s) 258
BlsI GCNGC 1 cut(s) 259
Bme1390I CCNGG 2 cut(s) 309, 605
Bme18I GGWCC 1 cut(s) 384
BmgT120I GGNCC 2 cut(s) 323, 384
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 2 cut(s) 309, 605
BmtI GCTAGC 1 cut(s) 11
Bpu10I CCTNAGC 1 cut(s) 249
BpuMI CCSGG 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 70, 612
Bsc4I CCNNNNNNNGG 1 cut(s) 631
BseBI CCWGG 1 cut(s) 605
BseDI CCNNGG 2 cut(s) 70, 612
BseGI GGATG 1 cut(s) 317
BseLI CCNNNNNNNGG 1 cut(s) 631
BseMII CTCAG 1 cut(s) 240
BseXI GCAGC 1 cut(s) 244
BsgI GTGCAG 1 cut(s) 594
BshFI GGCC 1 cut(s) 324
BsiSI CCGG 1 cut(s) 308
BslFI GGGAC 1 cut(s) 397
BslI CCNNNNNNNGG 1 cut(s) 631
BsmAI GTCTC 1 cut(s) 666
BsmFI GGGAC 1 cut(s) 397
BsnI GGCC 1 cut(s) 324
Bsp143I GATC 3 cut(s) 74, 193, 318
BspACI CCGC 1 cut(s) 650
BspANI GGCC 1 cut(s) 324
BspCNI CTCAG 1 cut(s) 241
BspLI GGNNCC 1 cut(s) 385
BspOI GCTAGC 1 cut(s) 11
BspPI GGATC 2 cut(s) 82, 188
BssECI CCNNGG 2 cut(s) 70, 612
BssMI GATC 3 cut(s) 74, 193, 318
BssNAI GTATAC 1 cut(s) 645
BssT1I CCWWGG 2 cut(s) 70, 612
Bst1107I GTATAC 1 cut(s) 645
Bst2UI CCWGG 1 cut(s) 605
Bst4CI ACNGT 2 cut(s) 512, 676
BstC8I GCNNGC 1 cut(s) 9
BstDEI CTNAG 2 cut(s) 249, 350
BstF5I GGATG 1 cut(s) 317
BstH2I RGCGCY 1 cut(s) 458
BstHHI GCGC 1 cut(s) 457
BstKTI GATC 3 cut(s) 77, 196, 321
BstMAI GTCTC 1 cut(s) 666
BstMBI GATC 3 cut(s) 74, 193, 318
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 1 cut(s) 605
BstSCI CCNGG 2 cut(s) 307, 603
BstSFI CTRYAG 1 cut(s) 123
BstV1I GCAGC 1 cut(s) 244
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BstZ17I GTATAC 1 cut(s) 645
BsuI GTATCC 2 cut(s) 619, 664
BsuRI GGCC 1 cut(s) 324
BtsCI GGATG 1 cut(s) 317
BtsIMutI CAGTG 1 cut(s) 508
Cac8I GCNNGC 1 cut(s) 9
CaiI CAGNNNCTG 1 cut(s) 197
CfoI GCGC 1 cut(s) 457
Cfr13I GGNCC 2 cut(s) 323, 384
CsiI ACCWGGT 1 cut(s) 603
Csp6I GTAC 1 cut(s) 535
CviJI RGCY 8 cut(s) 11, 220, 248, 257, 324, 337, 473, 586
CviKI_1 RGCY 8 cut(s) 11, 220, 248, 257, 324, 337, 473, 586
CviQI GTAC 1 cut(s) 535
DdeI CTNAG 2 cut(s) 249, 350
DpnI GATC 3 cut(s) 76, 195, 320
DpnII GATC 3 cut(s) 74, 193, 318
Eco130I CCWWGG 2 cut(s) 70, 612
Eco47I GGWCC 1 cut(s) 384
Eco47III AGCGCT 1 cut(s) 456
Eco57I CTGAAG 1 cut(s) 686
EcoRII CCWGG 1 cut(s) 603
EcoT14I CCWWGG 2 cut(s) 70, 612
ErhI CCWWGG 2 cut(s) 70, 612
FaiI YATR 5 cut(s) 113, 125, 228, 447, 645
FalI AAGNNNNNCTT 3 cut(s) 204, 236, 686
FaqI GGGAC 1 cut(s) 397
FblI GTMKAC 1 cut(s) 644
Fnu4HI GCNGC 1 cut(s) 258
FokI GGATG 1 cut(s) 324
Fsp4HI GCNGC 1 cut(s) 258
FspBI CTAG 3 cut(s) 8, 452, 474
GlaI GCGC 1 cut(s) 456
GluI GCNGC 1 cut(s) 258
HaeII RGCGCY 1 cut(s) 458
HaeIII GGCC 1 cut(s) 324
HapII CCGG 1 cut(s) 308
HhaI GCGC 1 cut(s) 457
Hin6I GCGC 1 cut(s) 455
HinP1I GCGC 1 cut(s) 455
HindIII AAGCTT 1 cut(s) 218
HinfI GANTC 1 cut(s) 179
HpaII CCGG 1 cut(s) 308
HphI GGTGA 2 cut(s) 328, 593
Hpy166II GTNNAC 2 cut(s) 479, 645
Hpy188I TCNGA 5 cut(s) 193, 345, 369, 517, 666
Hpy188III TCNNGA 1 cut(s) 523
Hpy8I GTNNAC 2 cut(s) 479, 645
HpyAV CCTTC 3 cut(s) 46, 79, 661
HpyCH4III ACNGT 2 cut(s) 512, 676
HpyCH4IV ACGT 2 cut(s) 392, 481
HpyCH4V TGCA 4 cut(s) 137, 272, 443, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 2 cut(s) 249, 350
HpySE526I ACGT 2 cut(s) 392, 481
HspAI GCGC 1 cut(s) 455
Kzo9I GATC 3 cut(s) 74, 193, 318
LpnPI CCDG 9 cut(s) 11, 57, 210, 321, 561, 576, 590, 617, 638
Lsp1109I GCAGC 1 cut(s) 244
MabI ACCWGGT 1 cut(s) 603
MaeI CTAG 3 cut(s) 8, 452, 474
MaeII ACGT 2 cut(s) 392, 481
MalI GATC 3 cut(s) 76, 195, 320
MboI GATC 3 cut(s) 74, 193, 318
MboII GAAGA 3 cut(s) 111, 694, 700
MflI RGATCY 1 cut(s) 193
MluCI AATT 4 cut(s) 32, 36, 171, 285
MmeI TCCRAC 1 cut(s) 475
MnlI CCTC 4 cut(s) 162, 244, 336, 511
MroXI GAANNNNTTC 3 cut(s) 363, 393, 701
MseI TTAA 4 cut(s) 15, 35, 63, 141
MspA1I CMGCKG 1 cut(s) 652
MspI CCGG 1 cut(s) 308
MspR9I CCNGG 2 cut(s) 309, 605
MvaI CCWGG 1 cut(s) 605
MwoI GCNNNNNNNGC 1 cut(s) 254
NciI CCSGG 1 cut(s) 309
NdeII GATC 3 cut(s) 74, 193, 318
NheI GCTAGC 1 cut(s) 7
NlaIV GGNNCC 1 cut(s) 385
PdmI GAANNNNTTC 3 cut(s) 363, 393, 701
PfeI GAWTC 1 cut(s) 179
PflMI CCANNNNNTGG 1 cut(s) 631
PfoI TCCNGGA 1 cut(s) 307
PkrI GCNGC 1 cut(s) 259
PshBI ATTAAT 1 cut(s) 35
PsiI TTATAA 1 cut(s) 113
Psp1406I AACGTT 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 603
PspGI CCWGG 1 cut(s) 603
PspN4I GGNNCC 1 cut(s) 385
PspPI GGNCC 2 cut(s) 323, 384
PstNI CAGNNNCTG 1 cut(s) 197
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 1 cut(s) 536
RsaNI GTAC 1 cut(s) 535
SaqAI TTAA 4 cut(s) 15, 35, 63, 141
SatI GCNGC 1 cut(s) 258
Sau3AI GATC 3 cut(s) 74, 193, 318
Sau96I GGNCC 2 cut(s) 323, 384
ScrFI CCNGG 2 cut(s) 309, 605
SexAI ACCWGGT 1 cut(s) 603
SfcI CTRYAG 1 cut(s) 123
SinI GGWCC 1 cut(s) 384
Sse9I AATT 4 cut(s) 32, 36, 171, 285
SsiI CCGC 1 cut(s) 650
SspMI CTAG 3 cut(s) 8, 452, 474
StyD4I CCNGG 2 cut(s) 307, 603
StyI CCWWGG 2 cut(s) 70, 612
TaaI ACNGT 2 cut(s) 512, 676
TaiI ACGT 2 cut(s) 395, 484
TaqII GACCGA 1 cut(s) 401
TasI AATT 4 cut(s) 32, 36, 171, 285
TfiI GAWTC 1 cut(s) 179
Tru1I TTAA 4 cut(s) 15, 35, 63, 141
Tru9I TTAA 4 cut(s) 15, 35, 63, 141
TscAI CASTG 1 cut(s) 515
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 1 cut(s) 192
TspRI CASTG 1 cut(s) 515
Van91I CCANNNNNTGG 1 cut(s) 631
VpaK11BI GGWCC 1 cut(s) 384
VspI ATTAAT 1 cut(s) 35
XmiI GTMKAC 1 cut(s) 644
XmnI GAANNNNTTC 3 cut(s) 363, 393, 701
XspI CTAG 3 cut(s) 8, 452, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.