Rh5DG369300

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000539
Physical Location & Seq
Forward (+)
55407366 .. 55413410
6045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG369300.1

Sequence Viewer

Length: 969 bp
ATGAGCAACAAGGTGGTCTGCGTCACGGGTGCCTCCGGCTACATTGCTTCATGGCTCGTCAAGCTCCTTCTCCAACGCGGCTACACTGTCAAGGCCTCTGTTCGCAACCCAAATGATCCAACAAAGACGGAGCACTTGCTCGCACTTGATGGAGCTAAGGAGAGACTTCAACTTTTCAAAGCAGATCTATTGGAAGAAGGTTCTTTTGACTCTGCTGTTGAGGGCTGTGAGGGTGTTTTCCACACTGCATCTCCCTTTTATCATGACGTCACAGACCCAAAGGCGGAACTACTTGATCCTGCAGTAAAGGGAACACTTAATGTTCTTAACTCGTGTTCGAAATCCACATCTATCAAACGGGTAGTCTTAACATCTTCTATAGCCGCAGTTGCATATAATGGAAAGCCTCGAACTCCTGATGTAGTGGTTGATGAGACTTGGTTTACTGATCCAGATGTCTGTAAGGAATCAAAGCTATGGTATGTGCTTTCAAAGACTTTGGCGGAGGATGCTGCCTGGAAATTTGTAAAGGAGAAGGGAATTGACATGGTTACAATTAATCCTGCAATGGTGATCGGTCCTCTGTTACAGCCAACGCTAAATACAAGTGCTGCTGCAATTCTGAATATTATTAAGGGAGCTCGAACGTATCCAAATGCAAGTTTTGGATGGGTTAATGTCAAAGATGTTGCCAACGCACATGTTCAAGCATTTGAGATTCCTTCAGCTAGTGGAAGATATTGTTTAGTCGAGAGAGTTGCACACTTCACTGAAGTTCTGCAAATTATACATGAGCTGTACCCTGATTTGCAACTTCCAGAGAAATGTTCGGATGATAAACCTTTTGTGCCAACATATCAGGTGTCCAAAGAAAAGGCAAAGAGCTTGGGTATTGAGTTTATTCCATTAGACATTAGCCTCAAGGAAACAATTGAAAGCTTGAAGGAAAAGAGTTTCGTCAGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

322

Amino Acids

35.41

Weight (kDa)

5.93

Isoelectric Point (pI)

28.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 3 - 101 2e-10 NmrA-like family
Epimerase PF01370 6 - 243 3.7e-23 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 8 - 237 3.3e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 8 - 202 1.5e-12 Male sterility protein
GDP_Man_Dehyd PF16363 8 - 242 1.7e-11 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 10 - 141 3.1e-12 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
AccB1I GGYRCC 1 cut(s) 29
AccII CGCG 1 cut(s) 78
AciI CCGC 4 cut(s) 78, 284, 384, 503
AclWI GGATC 3 cut(s) 110, 290, 443
AcsI RAATTY 1 cut(s) 521
AcuI CTGAAG 2 cut(s) 708, 792
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 1 cut(s) 800
AfiI CCNNNNNNNGG 1 cut(s) 283
AflIII ACRYGT 1 cut(s) 700
AgsI TTSAA 6 cut(s) 170, 178, 492, 707, 935, 943
AjnI CCWGG 1 cut(s) 515
AluBI AGCT 9 cut(s) 64, 155, 475, 641, 728, 796, 885, 939, 963
AluI AGCT 9 cut(s) 64, 155, 475, 641, 728, 796, 885, 939, 963
Alw21I GWGCWC 2 cut(s) 135, 643
Alw26I GTCTC 2 cut(s) 157, 428
AlwI GGATC 3 cut(s) 110, 290, 443
AlwNI CAGNNNCTG 1 cut(s) 966
AoxI GGCC 1 cut(s) 93
ApeKI GCWGC 3 cut(s) 512, 611, 614
ApoI RAATTY 1 cut(s) 521
ArsI GACNNNNNNTTYG 2 cut(s) 348, 380
AseI ATTAAT 1 cut(s) 558
AspS9I GGNCC 1 cut(s) 578
AsuHPI GGTGA 1 cut(s) 583
AsuII TTCGAA 1 cut(s) 338
AvaII GGWCC 1 cut(s) 578
BanI GGYRCC 1 cut(s) 29
BanII GRGCYC 1 cut(s) 643
BauI CACGAG 1 cut(s) 331
Bbv12I GWGCWC 2 cut(s) 135, 643
BbvI GCAGC 3 cut(s) 499, 598, 601
BccI CCATC 2 cut(s) 143, 663
BcgI CGANNNNNNTGC 2 cut(s) 740, 774
BciT130I CCWGG 1 cut(s) 517
BciVI GTATCC 1 cut(s) 660
BcoDI GTCTC 2 cut(s) 157, 428
BfaI CTAG 1 cut(s) 729
BfmI CTRYAG 2 cut(s) 300, 378
BfuI GTATCC 1 cut(s) 660
BglII AGATCT 1 cut(s) 184
BisI GCNGC 5 cut(s) 79, 384, 513, 612, 615
BlsI GCNGC 5 cut(s) 80, 385, 514, 613, 616
Bme1390I CCNGG 1 cut(s) 517
Bme18I GGWCC 1 cut(s) 578
BmgT120I GGNCC 1 cut(s) 578
BmiI GGNNCC 1 cut(s) 31
BmrFI CCNGG 1 cut(s) 517
BmsI GCATC 2 cut(s) 257, 499
Bpu10I CCTNAGC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 905
BsaHI GRCGYC 1 cut(s) 267
BsaXI ACNNNNNCTCC 2 cut(s) 235, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 283
Bse3DI GCAATG 2 cut(s) 42, 573
BseBI CCWGG 1 cut(s) 517
BseGI GGATG 3 cut(s) 514, 674, 838
BseLI CCNNNNNNNGG 1 cut(s) 283
BseMI GCAATG 2 cut(s) 42, 573
BseXI GCAGC 3 cut(s) 499, 598, 601
Bsh1236I CGCG 1 cut(s) 78
BshFI GGCC 1 cut(s) 95
BshNI GGYRCC 1 cut(s) 29
BsiHKAI GWGCWC 2 cut(s) 135, 643
BsiSI CCGG 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 283
BsmAI GTCTC 2 cut(s) 157, 428
BsnI GGCC 1 cut(s) 95
Bsp119I TTCGAA 1 cut(s) 338
Bsp1286I GDGCHC 2 cut(s) 135, 643
Bsp143I GATC 5 cut(s) 115, 184, 295, 448, 573
BspACI CCGC 4 cut(s) 78, 284, 384, 503
BspANI GGCC 1 cut(s) 95
BspFNI CGCG 1 cut(s) 78
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 1 cut(s) 31
BspMAI CTGCAG 1 cut(s) 304
BspPI GGATC 3 cut(s) 110, 290, 443
BspT104I TTCGAA 1 cut(s) 338
BspT107I GGYRCC 1 cut(s) 29
BsrDI GCAATG 2 cut(s) 42, 573
BssMI GATC 5 cut(s) 115, 184, 295, 448, 573
BssNI GRCGYC 1 cut(s) 267
BssSI CACGAG 1 cut(s) 331
Bst2BI CACGAG 1 cut(s) 331
Bst2UI CCWGG 1 cut(s) 517
Bst4CI ACNGT 1 cut(s) 88
BstACI GRCGYC 1 cut(s) 267
BstBI TTCGAA 1 cut(s) 338
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 1 cut(s) 156
BstF5I GGATG 3 cut(s) 514, 674, 838
BstFNI CGCG 1 cut(s) 78
BstKTI GATC 5 cut(s) 118, 187, 298, 451, 576
BstMAI GTCTC 2 cut(s) 157, 428
BstMBI GATC 5 cut(s) 115, 184, 295, 448, 573
BstMWI GCNNNNNNNGC 3 cut(s) 61, 389, 509
BstNI CCWGG 1 cut(s) 517
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 1 cut(s) 515
BstSFI CTRYAG 2 cut(s) 300, 378
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 3 cut(s) 499, 598, 601
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BsuI GTATCC 1 cut(s) 660
BsuRI GGCC 1 cut(s) 95
BtsCI GGATG 3 cut(s) 514, 674, 838
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 3 cut(s) 84, 243, 768
Cac8I GCNNGC 1 cut(s) 141
CaiI CAGNNNCTG 1 cut(s) 966
CciI TCATGA 1 cut(s) 262
Cfr13I GGNCC 1 cut(s) 578
CseI GACGC 1 cut(s) 10
Csp6I GTAC 1 cut(s) 799
CviAII CATG 5 cut(s) 51, 263, 547, 701, 791
CviQI GTAC 1 cut(s) 799
DdeI CTNAG 1 cut(s) 156
DpnI GATC 5 cut(s) 117, 186, 297, 450, 575
DpnII GATC 5 cut(s) 115, 184, 295, 448, 573
EciI GGCGGA 2 cut(s) 299, 518
Ecl136II GAGCTC 1 cut(s) 641
Eco147I AGGCCT 1 cut(s) 95
Eco24I GRGCYC 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 578
Eco53kI GAGCTC 1 cut(s) 641
Eco57I CTGAAG 2 cut(s) 708, 792
EcoICRI GAGCTC 1 cut(s) 641
EcoRII CCWGG 1 cut(s) 515
EcoT38I GRGCYC 1 cut(s) 643
FaeI CATG 5 cut(s) 54, 266, 550, 704, 794
FatI CATG 5 cut(s) 50, 262, 546, 700, 790
Fnu4HI GCNGC 5 cut(s) 79, 384, 513, 612, 615
FokI GGATG 3 cut(s) 521, 681, 845
FriOI GRGCYC 1 cut(s) 643
Fsp4HI GCNGC 5 cut(s) 79, 384, 513, 612, 615
FspBI CTAG 1 cut(s) 729
GluI GCNGC 5 cut(s) 79, 384, 513, 612, 615
HaeIII GGCC 1 cut(s) 95
HapII CCGG 1 cut(s) 36
HgaI GACGC 1 cut(s) 10
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 5 cut(s) 54, 266, 550, 704, 794
HindIII AAGCTT 1 cut(s) 937
HinfI GANTC 3 cut(s) 209, 467, 718
HpaII CCGG 1 cut(s) 36
HphI GGTGA 1 cut(s) 583
Hpy166II GTNNAC 1 cut(s) 444
Hpy188I TCNGA 3 cut(s) 624, 832, 968
Hpy188III TCNNGA 5 cut(s) 263, 416, 452, 751, 818
Hpy8I GTNNAC 1 cut(s) 444
HpyAV CCTTC 5 cut(s) 77, 191, 529, 732, 937
HpyCH4III ACNGT 1 cut(s) 88
HpyCH4IV ACGT 2 cut(s) 267, 647
HpyCH4V TGCA 9 cut(s) 248, 302, 392, 566, 617, 659, 761, 781, 811
HpyF10VI GCNNNNNNNGC 3 cut(s) 61, 389, 509
HpyF3I CTNAG 1 cut(s) 156
HpySE526I ACGT 2 cut(s) 267, 647
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 5 cut(s) 54, 266, 550, 704, 794
Kzo9I GATC 5 cut(s) 115, 184, 295, 448, 573
LmnI GCTCC 4 cut(s) 69, 130, 152, 638
Lsp1109I GCAGC 3 cut(s) 499, 598, 601
LweI GCATC 2 cut(s) 257, 499
MaeI CTAG 1 cut(s) 729
MaeII ACGT 2 cut(s) 267, 647
MaeIII GTNAC 4 cut(s) 22, 268, 550, 585
MalI GATC 5 cut(s) 117, 186, 297, 450, 575
MboI GATC 5 cut(s) 115, 184, 295, 448, 573
MboII GAAGA 3 cut(s) 206, 366, 747
MfeI CAATTG 1 cut(s) 930
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 2 cut(s) 135, 643
MluCI AATT 6 cut(s) 521, 540, 555, 618, 783, 930
MlyI GAGTC 1 cut(s) 203
MmeI TCCRAC 2 cut(s) 97, 143
MnlI CCTC 8 cut(s) 43, 106, 214, 223, 417, 499, 591, 929
MseI TTAA 6 cut(s) 318, 327, 368, 558, 633, 675
MspI CCGG 1 cut(s) 36
MspR9I CCNGG 1 cut(s) 517
MunI CAATTG 1 cut(s) 930
MvaI CCWGG 1 cut(s) 517
MvnI CGCG 1 cut(s) 78
MwoI GCNNNNNNNGC 3 cut(s) 61, 389, 509
NdeII GATC 5 cut(s) 115, 184, 295, 448, 573
NlaIII CATG 5 cut(s) 54, 266, 550, 704, 794
NlaIV GGNNCC 1 cut(s) 31
NmuCI GTSAC 2 cut(s) 22, 268
NspI RCATGY 1 cut(s) 704
NspV TTCGAA 1 cut(s) 338
PagI TCATGA 1 cut(s) 262
PceI AGGCCT 1 cut(s) 95
PciI ACATGT 1 cut(s) 700
PfeI GAWTC 2 cut(s) 467, 718
PkrI GCNGC 5 cut(s) 80, 385, 514, 613, 616
PleI GAGTC 1 cut(s) 203
PpsI GAGTC 1 cut(s) 203
PscI ACATGT 1 cut(s) 700
PshBI ATTAAT 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 643
Psp6I CCWGG 1 cut(s) 515
PspGI CCWGG 1 cut(s) 515
PspN4I GGNNCC 1 cut(s) 31
PspPI GGNCC 1 cut(s) 578
PstI CTGCAG 1 cut(s) 304
PstNI CAGNNNCTG 1 cut(s) 966
PsuI RGATCY 1 cut(s) 184
RsaI GTAC 1 cut(s) 800
RsaNI GTAC 1 cut(s) 799
SacI GAGCTC 1 cut(s) 643
SaqAI TTAA 6 cut(s) 318, 327, 368, 558, 633, 675
SatI GCNGC 5 cut(s) 79, 384, 513, 612, 615
Sau3AI GATC 5 cut(s) 115, 184, 295, 448, 573
Sau96I GGNCC 1 cut(s) 578
SchI GAGTC 1 cut(s) 203
ScrFI CCNGG 1 cut(s) 517
SduI GDGCHC 2 cut(s) 135, 643
SfaNI GCATC 2 cut(s) 257, 499
SfcI CTRYAG 2 cut(s) 300, 378
SfuI TTCGAA 1 cut(s) 338
SinI GGWCC 1 cut(s) 578
SmlI CTYRAG 1 cut(s) 920
SmoI CTYRAG 1 cut(s) 920
Sse9I AATT 6 cut(s) 521, 540, 555, 618, 783, 930
SseBI AGGCCT 1 cut(s) 95
SsiI CCGC 4 cut(s) 78, 284, 384, 503
SspI AATATT 1 cut(s) 628
SspMI CTAG 1 cut(s) 729
SstI GAGCTC 1 cut(s) 643
StuI AGGCCT 1 cut(s) 95
StyD4I CCNGG 1 cut(s) 515
TaaI ACNGT 1 cut(s) 88
TaiI ACGT 2 cut(s) 270, 650
TaqI TCGA 4 cut(s) 338, 409, 643, 750
TaqII GACCGA 1 cut(s) 566
TasI AATT 6 cut(s) 521, 540, 555, 618, 783, 930
TauI GCSGC 2 cut(s) 81, 386
TfiI GAWTC 2 cut(s) 467, 718
Tru1I TTAA 6 cut(s) 318, 327, 368, 558, 633, 675
Tru9I TTAA 6 cut(s) 318, 327, 368, 558, 633, 675
TscAI CASTG 3 cut(s) 91, 250, 775
TseFI GTSAC 2 cut(s) 22, 268
TseI GCWGC 3 cut(s) 512, 611, 614
Tsp45I GTSAC 2 cut(s) 22, 268
TspDTI ATGAA 1 cut(s) 39
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 3 cut(s) 91, 250, 775
VpaK11BI GGWCC 1 cut(s) 578
VspI ATTAAT 1 cut(s) 558
XapI RAATTY 1 cut(s) 521
XceI RCATGY 1 cut(s) 704
XspI CTAG 1 cut(s) 729
ZraI GACGTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.