RchiOBHm_Chr1g0316841

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000539
Physical Location & Seq
Forward (+)
4334205 .. 4336698
2494 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54723

Sequence Viewer

Length: 855 bp
ATGAGCTGCGGAGAAAGCAAGGTTGTGTGTGTGACAGGAGCATCTGGGTTCATAGCATCATGGCTAGTCAAGCTCTTATTGCAACGAGGTTATACTGTCAAAGCCACTGTTCGGGACCCAAATGATCAAAAGAAAACAGAGCACCTACTTTCATTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGAAGAAGGTTCTTTTGACCCTGTCGTTGATGGGTGTGAAGGTGCAGAATTAATTGAGCCTGCTTTGAAGGGAACCCTTAATGTCCTTGGATCGTGTGTGAAGGTCCAGTCTATCAAAAGGGTGGTTATAACATCCTCTATGGCAGCAGTTAGATTTAATGGAAAACCTCTTACTGCTGATGTAATAATCAATGAATCTTGGTTTTCAGATCCTGCTTTTTGTGAAAAAGAAAAGCTTTGGTATATGCTTTCAAAGACGTTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAGAAAGGAATTGATATTATTACAATAAATCCGGGATGGGTGATCGGCCCTCCCTTACAGCCAACTCTGAACTTAACTGTGGAACTAGTTCTGAAACTCGTAAATGGGACCGAAAAGTTTCCCAACGACACCTACAGATTTGTTGATGTTAGAGATGTTGCTAATGCACATATTCTAGCCTTTGAAAACCCATCTGCTAGTGGACGTTATTGTTTAGTTGGAAGCGTAAAACACCGTTCAGAGTCTCTATTTTTGACAGATGTGAAGATGACAAGCCTTTCACACCAACTCACCAGACATACTCCGCTTGAAGTGTCTCTGAAGGATACTGTTGAAAGTTTGAAGGACAAGAACTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

284

Amino Acids

31.46

Weight (kDa)

6.61

Isoelectric Point (pI)

27.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 81 2.1e-06 NmrA-like family
Epimerase PF01370 9 - 231 1.5e-15 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 11 - 80 4.2e-08 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 118 1.2e-06 NAD(P)H-binding
3Beta_HSD PF01073 88 - 226 9.4e-07 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 329
AciI CCGC 2 cut(s) 9, 797
AclWI GGATC 2 cut(s) 298, 404
AcuI CTGAAG 1 cut(s) 833
AfiI CCNNNNNNNGG 1 cut(s) 111
AgsI TTSAA 7 cut(s) 187, 268, 453, 677, 803, 827, 835
AhlI ACTAGT 1 cut(s) 577
AluBI AGCT 5 cut(s) 6, 73, 436, 464, 473
AluI AGCT 5 cut(s) 6, 73, 436, 464, 473
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 2 cut(s) 741, 813
AlwI GGATC 2 cut(s) 298, 404
AlwNI CAGNNNCTG 1 cut(s) 413
AoxI GGCC 1 cut(s) 538
ApeKI GCWGC 3 cut(s) 6, 344, 473
AseI ATTAAT 1 cut(s) 251
Asp700I GAANNNNTTC 4 cut(s) 174, 579, 609, 848
AspS9I GGNCC 4 cut(s) 115, 304, 539, 600
AsuC2I CCSGG 1 cut(s) 525
AsuHPI GGTGA 2 cut(s) 544, 775
AvaII GGWCC 3 cut(s) 115, 304, 600
Bbv12I GWGCWC 1 cut(s) 144
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 2 cut(s) 356, 460
BccI CCATC 4 cut(s) 152, 224, 522, 691
BciVI GTATCC 1 cut(s) 811
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 1 cut(s) 525
BcoDI GTCTC 2 cut(s) 741, 813
BcuI ACTAGT 1 cut(s) 577
BfaI CTAG 4 cut(s) 65, 578, 668, 690
BfmI CTRYAG 1 cut(s) 625
BfuI GTATCC 1 cut(s) 811
BisI GCNGC 3 cut(s) 7, 345, 474
BlsI GCNGC 3 cut(s) 8, 346, 475
Bme1390I CCNGG 1 cut(s) 525
Bme18I GGWCC 3 cut(s) 115, 304, 600
BmgT120I GGNCC 4 cut(s) 115, 304, 539, 600
BmiI GGNNCC 4 cut(s) 116, 117, 274, 601
BmrFI CCNGG 1 cut(s) 525
BmsI GCATC 2 cut(s) 50, 65
Bpu10I CCTNAGC 1 cut(s) 465
BpuMI CCSGG 1 cut(s) 525
BsaJI CCNNGG 1 cut(s) 286
Bsc4I CCNNNNNNNGG 1 cut(s) 111
Bse1I ACTGG 1 cut(s) 307
BseDI CCNNGG 1 cut(s) 286
BseGI GGATG 2 cut(s) 332, 533
BseLI CCNNNNNNNGG 1 cut(s) 111
BseMII CTCAG 1 cut(s) 456
BseNI ACTGG 1 cut(s) 307
BseXI GCAGC 2 cut(s) 356, 460
BsgI GTGCAG 1 cut(s) 264
BshFI GGCC 1 cut(s) 540
BsiHKAI GWGCWC 1 cut(s) 144
BsiSI CCGG 1 cut(s) 524
BslFI GGGAC 2 cut(s) 128, 613
BslI CCNNNNNNNGG 1 cut(s) 111
BsmAI GTCTC 2 cut(s) 741, 813
BsmFI GGGAC 2 cut(s) 128, 613
BsnI GGCC 1 cut(s) 540
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 4 cut(s) 124, 290, 409, 534
BspACI CCGC 2 cut(s) 9, 797
BspANI GGCC 1 cut(s) 540
BspCNI CTCAG 1 cut(s) 457
BspLI GGNNCC 4 cut(s) 116, 117, 274, 601
BspPI GGATC 2 cut(s) 298, 404
BsrI ACTGG 1 cut(s) 307
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 4 cut(s) 124, 290, 409, 534
BssT1I CCWWGG 1 cut(s) 286
Bst4CI ACNGT 5 cut(s) 97, 109, 571, 728, 823
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 1 cut(s) 465
BstF5I GGATG 2 cut(s) 332, 533
BstKTI GATC 4 cut(s) 127, 293, 412, 537
BstMAI GTCTC 2 cut(s) 741, 813
BstMBI GATC 4 cut(s) 124, 290, 409, 534
BstMWI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 470
BstSCI CCNGG 1 cut(s) 523
BstSFI CTRYAG 1 cut(s) 625
BstV1I GCAGC 2 cut(s) 356, 460
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
BsuI GTATCC 1 cut(s) 811
BsuRI GGCC 1 cut(s) 540
BtsCI GGATG 2 cut(s) 332, 533
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 261
CaiI CAGNNNCTG 1 cut(s) 413
Cfr13I GGNCC 4 cut(s) 115, 304, 539, 600
CviAII CATG 1 cut(s) 60
DdeI CTNAG 1 cut(s) 465
DpnI GATC 4 cut(s) 126, 292, 411, 536
DpnII GATC 4 cut(s) 124, 290, 409, 534
Eco130I CCWWGG 1 cut(s) 286
Eco47I GGWCC 3 cut(s) 115, 304, 600
Eco57I CTGAAG 1 cut(s) 833
EcoO109I RGGNCCY 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 1 cut(s) 63
FaiI YATR 9 cut(s) 53, 61, 93, 329, 341, 444, 446, 663, 792
FalI AAGNNNNNCTT 3 cut(s) 420, 452, 833
FaqI GGGAC 2 cut(s) 128, 613
FatI CATG 1 cut(s) 59
FbaI TGATCA 1 cut(s) 124
Fnu4HI GCNGC 3 cut(s) 7, 345, 474
FokI GGATG 2 cut(s) 319, 540
Fsp4HI GCNGC 3 cut(s) 7, 345, 474
FspBI CTAG 4 cut(s) 65, 578, 668, 690
GluI GCNGC 3 cut(s) 7, 345, 474
HaeIII GGCC 1 cut(s) 540
HapII CCGG 1 cut(s) 524
Hin1II CATG 1 cut(s) 63
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 2 cut(s) 395, 734
HpaII CCGG 1 cut(s) 524
HphI GGTGA 2 cut(s) 544, 775
Hpy166II GTNNAC 1 cut(s) 695
Hpy188I TCNGA 5 cut(s) 409, 561, 585, 733, 813
Hpy188III TCNNGA 1 cut(s) 113
Hpy8I GTNNAC 1 cut(s) 695
HpyAV CCTTC 6 cut(s) 200, 233, 262, 295, 808, 829
HpyCH4III ACNGT 5 cut(s) 97, 109, 571, 728, 823
HpyCH4IV ACGT 2 cut(s) 458, 697
HpyCH4V TGCA 4 cut(s) 82, 245, 488, 659
HpyF10VI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 470
HpyF3I CTNAG 1 cut(s) 465
HpySE526I ACGT 2 cut(s) 458, 697
Hsp92II CATG 1 cut(s) 63
KflI GGGWCCC 1 cut(s) 115
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 4 cut(s) 124, 290, 409, 534
LmnI GCTCC 2 cut(s) 38, 161
LpnPI CCDG 8 cut(s) 21, 30, 234, 273, 320, 426, 537, 799
Lsp1109I GCAGC 2 cut(s) 356, 460
LweI GCATC 2 cut(s) 50, 65
MaeI CTAG 4 cut(s) 65, 578, 668, 690
MaeII ACGT 2 cut(s) 458, 697
MaeIII GTNAC 1 cut(s) 31
MalI GATC 4 cut(s) 126, 292, 411, 536
MboI GATC 4 cut(s) 124, 290, 409, 534
MboII GAAGA 3 cut(s) 215, 769, 841
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 3 cut(s) 248, 252, 501
MlyI GAGTC 1 cut(s) 743
MmeI TCCRAC 1 cut(s) 691
MnlI CCTC 5 cut(s) 80, 346, 378, 460, 552
MroXI GAANNNNTTC 4 cut(s) 174, 579, 609, 848
MseI TTAA 4 cut(s) 251, 279, 357, 566
MspI CCGG 1 cut(s) 524
MspR9I CCNGG 1 cut(s) 525
MwoI GCNNNNNNNGC 4 cut(s) 15, 70, 79, 470
NciI CCSGG 1 cut(s) 525
NdeII GATC 4 cut(s) 124, 290, 409, 534
NlaIII CATG 1 cut(s) 63
NlaIV GGNNCC 4 cut(s) 116, 117, 274, 601
NmuCI GTSAC 1 cut(s) 31
PdmI GAANNNNTTC 4 cut(s) 174, 579, 609, 848
PfeI GAWTC 1 cut(s) 395
PflFI GACNNNGTC 1 cut(s) 221
PfoI TCCNGGA 1 cut(s) 523
PkrI GCNGC 3 cut(s) 8, 346, 475
PleI GAGTC 1 cut(s) 742
PpsI GAGTC 1 cut(s) 742
PpuMI RGGWCCY 1 cut(s) 115
PshBI ATTAAT 1 cut(s) 251
PsiI TTATAA 1 cut(s) 329
Psp5II RGGWCCY 1 cut(s) 115
PspN4I GGNNCC 4 cut(s) 116, 117, 274, 601
PspPI GGNCC 4 cut(s) 115, 304, 539, 600
PspPPI RGGWCCY 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 413
PsuI RGATCY 1 cut(s) 409
PsyI GACNNNGTC 1 cut(s) 221
SaqAI TTAA 4 cut(s) 251, 279, 357, 566
SatI GCNGC 3 cut(s) 7, 345, 474
Sau3AI GATC 4 cut(s) 124, 290, 409, 534
Sau96I GGNCC 4 cut(s) 115, 304, 539, 600
SchI GAGTC 1 cut(s) 743
ScrFI CCNGG 1 cut(s) 525
SduI GDGCHC 1 cut(s) 144
SfaNI GCATC 2 cut(s) 50, 65
SfcI CTRYAG 1 cut(s) 625
SinI GGWCC 3 cut(s) 115, 304, 600
SpeI ACTAGT 1 cut(s) 577
Sse9I AATT 3 cut(s) 248, 252, 501
SsiI CCGC 2 cut(s) 9, 797
SspMI CTAG 4 cut(s) 65, 578, 668, 690
StyD4I CCNGG 1 cut(s) 523
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 5 cut(s) 97, 109, 571, 728, 823
TaiI ACGT 2 cut(s) 461, 700
TaqII GACCGA 1 cut(s) 617
TasI AATT 3 cut(s) 248, 252, 501
TfiI GAWTC 1 cut(s) 395
Tru1I TTAA 4 cut(s) 251, 279, 357, 566
Tru9I TTAA 4 cut(s) 251, 279, 357, 566
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 3 cut(s) 6, 344, 473
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 4 cut(s) 40, 141, 167, 408
TspRI CASTG 1 cut(s) 112
Tth111I GACNNNGTC 1 cut(s) 221
VpaK11BI GGWCC 3 cut(s) 115, 304, 600
VspI ATTAAT 1 cut(s) 251
XmnI GAANNNNTTC 4 cut(s) 174, 579, 609, 848
XspI CTAG 4 cut(s) 65, 578, 668, 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.