MD02G1302900.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000539
Physical Location & Seq
Forward (+)
35689520 .. 35693804
4285 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1302900.v1.1.491

Sequence Viewer

Length: 969 bp
ATGAGCGGAGGAAGTAAAGTGGTTTGTGTAACAGGAGCATCCGGGTTCATAGCATCATGGGTGGTGAAGTTCCTACTGCAAAAGGGTTATACTGTCAAAGCCACTGTGCGTGACCCAAATGATCCAAAGAAAACACAACACTTACTCTCACTAGATGGAGCAAAAGAAAGGCTTCATTTGTTCAAAGCAGATTTATTAGAAGAGGGATCTTTTGACGCTGTCGTTGATGGATGTGAAGGAGTTTTTCATATGGCATCGCCTGTACTAATGTCAGTCACTGACCCACAGGCAGAACTACTTGACCCTGCTGTGAAGGGAACACTGAATGTCCTCAGATCATGCGCGAAATTTCCGTCTATCAAAAGGGTGGTTCTAACATCCTCCATAGTATCAATTGTATTTACTGGAAAGCCTCTTTCTGCTGATACTGTAGTTGATGAATCTTGGTTTTCAGATCCAGCTTTCTGTGAAAAAGCAAAGCTTTGGTATGCACTTTCAAAAACCTTAGCTGAGGAAGCTGCTTGGAAGTTTGTGAAGGAGAATGGAATCGATTTAGTTGCAGTAAATCCAGGGGTGGCGATTGGCCCTCTTTTACAGCCAACTCTGAACTCAAGCTCGGAGCTACTTCTCAAACTCTTTGATGGAACCGGAAAATTTCCAAATGCAACTCACAGATGGGTTGATGTTAGAGATATTGCTAATGCACATATTCTAGCCTTTGAGAATCCTTCAGCTAGTGGGAGGTATTCTATAGTTGGAAGTGTAGCACACTATTCTGTGGCCGTTAAAATTCTTCGTGATCTCTTCCCTGCTCTCAATCTTCCGGAAAAATGTGCAGATGATCAACCTTTCGCACCAACTTACCAGATATCCAAGGAAAGAGCTGAGACTTTGGGTGTTGTGTATACTCCTCTGGATGTGACTTTGAAGGACGCTGTTGAAAGTTTGAAGCAGAAGAACTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

323

Amino Acids

34.97

Weight (kDa)

6.32

Isoelectric Point (pI)

30.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 101 1.6e-09 NmrA-like family
Epimerase PF01370 8 - 248 7.4e-23 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 242 1.1e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 241 4.1e-13 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 199 9.3e-12 Male sterility protein
NAD_binding_10 PF13460 12 - 129 1.6e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 905
AccII CGCG 1 cut(s) 344
AccIII TCCGGA 1 cut(s) 823
AciI CCGC 1 cut(s) 6
AclWI GGATC 3 cut(s) 116, 214, 449
AcoI YGGCCR 1 cut(s) 780
AcsI RAATTY 3 cut(s) 347, 653, 789
AcuI CTGAAG 1 cut(s) 714
AfaI GTAC 1 cut(s) 264
AgsI TTSAA 5 cut(s) 184, 498, 928, 941, 949
AjnI CCWGG 1 cut(s) 568
AluBI AGCT 8 cut(s) 461, 481, 509, 518, 615, 622, 734, 884
AluI AGCT 8 cut(s) 461, 481, 509, 518, 615, 622, 734, 884
Alw26I GTCTC 1 cut(s) 881
AlwI GGATC 3 cut(s) 116, 214, 449
AlwNI CAGNNNCTG 1 cut(s) 278
Aor13HI TCCGGA 1 cut(s) 823
AoxI GGCC 2 cut(s) 583, 780
ApeKI GCWGC 1 cut(s) 518
ApoI RAATTY 3 cut(s) 347, 653, 789
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
Asp700I GAANNNNTTC 3 cut(s) 171, 959, 962
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 1 cut(s) 584
AsuC2I CCSGG 1 cut(s) 43
AsuHPI GGTGA 1 cut(s) 76
BaeI ACNNNNGTAYC 2 cut(s) 417, 450
BbvCI CCTCAGC 1 cut(s) 510
BbvI GCAGC 1 cut(s) 505
BccI CCATC 4 cut(s) 149, 221, 635, 669
BceAI ACGGC 1 cut(s) 767
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 1 cut(s) 570
BclI TGATCA 1 cut(s) 841
BcnI CCSGG 1 cut(s) 43
BcoDI GTCTC 1 cut(s) 881
BfaI CTAG 3 cut(s) 152, 713, 735
BfmI CTRYAG 2 cut(s) 429, 750
BisI GCNGC 1 cut(s) 519
BlsI GCNGC 1 cut(s) 520
Bme1390I CCNGG 2 cut(s) 43, 570
BmgT120I GGNCC 1 cut(s) 584
BmiI GGNNCC 1 cut(s) 646
BmrFI CCNGG 2 cut(s) 43, 570
BmsI GCATC 3 cut(s) 47, 62, 263
Bpu10I CCTNAGC 2 cut(s) 505, 510
BpuEI CTTGAG 1 cut(s) 595
BpuMI CCSGG 1 cut(s) 43
Bsa29I ATCGAT 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 569, 873
BsaWI WCCGGW 2 cut(s) 647, 823
Bse1I ACTGG 1 cut(s) 409
BseAI TCCGGA 1 cut(s) 823
BseBI CCWGG 1 cut(s) 570
BseCI ATCGAT 1 cut(s) 549
BseDI CCNNGG 2 cut(s) 569, 873
BseGI GGATG 4 cut(s) 38, 236, 377, 922
BseMII CTCAG 3 cut(s) 346, 501, 876
BseNI ACTGG 1 cut(s) 409
BseRI GAGGAG 1 cut(s) 900
BseXI GCAGC 1 cut(s) 505
BsgI GTGCAG 1 cut(s) 855
Bsh1236I CGCG 1 cut(s) 344
BshFI GGCC 2 cut(s) 585, 782
BshVI ATCGAT 1 cut(s) 549
BsiSI CCGG 3 cut(s) 42, 648, 824
BsmAI GTCTC 1 cut(s) 881
BsnI GGCC 2 cut(s) 585, 782
Bsp13I TCCGGA 1 cut(s) 823
Bsp143I GATC 6 cut(s) 121, 206, 335, 454, 799, 841
BspACI CCGC 1 cut(s) 6
BspANI GGCC 2 cut(s) 585, 782
BspCNI CTCAG 3 cut(s) 345, 502, 877
BspDI ATCGAT 1 cut(s) 549
BspEI TCCGGA 1 cut(s) 823
BspFNI CGCG 1 cut(s) 344
BspLI GGNNCC 1 cut(s) 646
BspPI GGATC 3 cut(s) 116, 214, 449
BsrBI CCGCTC 1 cut(s) 6
BsrI ACTGG 1 cut(s) 409
BssECI CCNNGG 2 cut(s) 569, 873
BssMI GATC 6 cut(s) 121, 206, 335, 454, 799, 841
BssNAI GTATAC 1 cut(s) 906
BssT1I CCWWGG 1 cut(s) 873
Bst1107I GTATAC 1 cut(s) 906
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 3 cut(s) 94, 106, 430
Bst6I CTCTTC 2 cut(s) 195, 809
BstDEI CTNAG 4 cut(s) 332, 505, 510, 885
BstF5I GGATG 4 cut(s) 38, 236, 377, 922
BstFNI CGCG 1 cut(s) 344
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 6 cut(s) 124, 209, 338, 457, 802, 844
BstMAI GTCTC 1 cut(s) 881
BstMBI GATC 6 cut(s) 121, 206, 335, 454, 799, 841
BstMWI GCNNNNNNNGC 1 cut(s) 515
BstNI CCWGG 1 cut(s) 570
BstSCI CCNGG 2 cut(s) 41, 568
BstSFI CTRYAG 2 cut(s) 429, 750
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 1 cut(s) 505
BstX2I RGATCY 2 cut(s) 206, 454
BstYI RGATCY 2 cut(s) 206, 454
BstZ17I GTATAC 1 cut(s) 906
Bsu15I ATCGAT 1 cut(s) 549
BsuRI GGCC 2 cut(s) 585, 782
BsuTUI ATCGAT 1 cut(s) 549
BtgZI GCGATG 1 cut(s) 240
BtsCI GGATG 4 cut(s) 38, 236, 377, 922
BtsIMutI CAGTG 3 cut(s) 102, 276, 320
CaiI CAGNNNCTG 1 cut(s) 278
CfoI GCGC 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 584
ClaI ATCGAT 1 cut(s) 549
CseI GACGC 2 cut(s) 224, 941
Csp6I GTAC 1 cut(s) 263
CviAII CATG 2 cut(s) 57, 339
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 4 cut(s) 332, 505, 510, 885
DpnI GATC 6 cut(s) 123, 208, 337, 456, 801, 843
DpnII GATC 6 cut(s) 121, 206, 335, 454, 799, 841
EaeI YGGCCR 1 cut(s) 780
Eam1104I CTCTTC 2 cut(s) 195, 809
EarI CTCTTC 2 cut(s) 195, 809
Eco130I CCWWGG 1 cut(s) 873
Eco32I GATATC 1 cut(s) 870
Eco57I CTGAAG 1 cut(s) 714
EcoRII CCWGG 1 cut(s) 568
EcoRV GATATC 1 cut(s) 870
EcoT14I CCWWGG 1 cut(s) 873
ErhI CCWWGG 1 cut(s) 873
FaeI CATG 2 cut(s) 60, 342
FalI AAGNNNNNCTT 5 cut(s) 156, 188, 465, 497, 947
FatI CATG 2 cut(s) 56, 338
FauNDI CATATG 1 cut(s) 249
FbaI TGATCA 1 cut(s) 841
FblI GTMKAC 1 cut(s) 905
Fnu4HI GCNGC 1 cut(s) 519
FokI GGATG 4 cut(s) 25, 243, 364, 929
Fsp4HI GCNGC 1 cut(s) 519
FspBI CTAG 3 cut(s) 152, 713, 735
GlaI GCGC 1 cut(s) 343
GluI GCNGC 1 cut(s) 519
HaeIII GGCC 2 cut(s) 585, 782
HapII CCGG 3 cut(s) 42, 648, 824
HgaI GACGC 2 cut(s) 224, 941
HhaI GCGC 1 cut(s) 344
Hin1II CATG 2 cut(s) 60, 342
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HindIII AAGCTT 1 cut(s) 479
HinfI GANTC 3 cut(s) 440, 546, 724
HpaII CCGG 3 cut(s) 42, 648, 824
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 1 cut(s) 906
Hpy188I TCNGA 4 cut(s) 335, 454, 606, 619
Hpy188III TCNNGA 3 cut(s) 797, 824, 914
Hpy8I GTNNAC 1 cut(s) 906
HpyAV CCTTC 5 cut(s) 230, 307, 529, 738, 922
HpyCH4III ACNGT 3 cut(s) 94, 106, 430
HpyCH4V TGCA 6 cut(s) 79, 491, 560, 665, 704, 836
HpyF10VI GCNNNNNNNGC 1 cut(s) 515
HpyF3I CTNAG 4 cut(s) 332, 505, 510, 885
Hsp92II CATG 2 cut(s) 60, 342
HspAI GCGC 1 cut(s) 342
Kpn2I TCCGGA 1 cut(s) 823
Ksp22I TGATCA 1 cut(s) 841
Kzo9I GATC 6 cut(s) 121, 206, 335, 454, 799, 841
LmnI GCTCC 3 cut(s) 35, 158, 619
Lsp1109I GCAGC 1 cut(s) 505
LweI GCATC 3 cut(s) 47, 62, 263
MaeI CTAG 3 cut(s) 152, 713, 735
MaeIII GTNAC 4 cut(s) 28, 110, 274, 919
MalI GATC 6 cut(s) 123, 208, 337, 456, 801, 843
MbiI CCGCTC 1 cut(s) 6
MboI GATC 6 cut(s) 121, 206, 335, 454, 799, 841
MboII GAAGA 6 cut(s) 212, 785, 796, 812, 955, 967
MfeI CAATTG 1 cut(s) 393
MflI RGATCY 2 cut(s) 206, 454
MluCI AATT 4 cut(s) 347, 393, 653, 789
MmeI TCCRAC 1 cut(s) 736
MnlI CCTC 8 cut(s) 196, 341, 391, 423, 505, 597, 735, 921
MroI TCCGGA 1 cut(s) 823
MroXI GAANNNNTTC 3 cut(s) 171, 959, 962
MseI TTAA 1 cut(s) 786
MspI CCGG 3 cut(s) 42, 648, 824
MspR9I CCNGG 2 cut(s) 43, 570
MunI CAATTG 1 cut(s) 393
MvaI CCWGG 1 cut(s) 570
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 1 cut(s) 515
NciI CCSGG 1 cut(s) 43
NdeI CATATG 1 cut(s) 249
NdeII GATC 6 cut(s) 121, 206, 335, 454, 799, 841
NlaIII CATG 2 cut(s) 60, 342
NlaIV GGNNCC 1 cut(s) 646
NmuCI GTSAC 3 cut(s) 110, 274, 919
PdmI GAANNNNTTC 3 cut(s) 171, 959, 962
PfeI GAWTC 3 cut(s) 440, 546, 724
PflFI GACNNNGTC 1 cut(s) 218
PkrI GCNGC 1 cut(s) 520
Psp6I CCWGG 1 cut(s) 568
PspGI CCWGG 1 cut(s) 568
PspN4I GGNNCC 1 cut(s) 646
PspPI GGNCC 1 cut(s) 584
PstNI CAGNNNCTG 1 cut(s) 278
PsuI RGATCY 2 cut(s) 206, 454
PsyI GACNNNGTC 1 cut(s) 218
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
SaqAI TTAA 1 cut(s) 786
SatI GCNGC 1 cut(s) 519
Sau3AI GATC 6 cut(s) 121, 206, 335, 454, 799, 841
Sau96I GGNCC 1 cut(s) 584
ScrFI CCNGG 2 cut(s) 43, 570
SfaNI GCATC 3 cut(s) 47, 62, 263
SfcI CTRYAG 2 cut(s) 429, 750
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
Sse9I AATT 4 cut(s) 347, 393, 653, 789
SsiI CCGC 1 cut(s) 6
SspMI CTAG 3 cut(s) 152, 713, 735
StyD4I CCNGG 2 cut(s) 41, 568
StyI CCWWGG 1 cut(s) 873
TaaI ACNGT 3 cut(s) 94, 106, 430
TaqI TCGA 1 cut(s) 549
TasI AATT 4 cut(s) 347, 393, 653, 789
TatI WGTACW 1 cut(s) 262
TfiI GAWTC 3 cut(s) 440, 546, 724
Tru1I TTAA 1 cut(s) 786
Tru9I TTAA 1 cut(s) 786
TscAI CASTG 3 cut(s) 109, 283, 327
TseFI GTSAC 3 cut(s) 110, 274, 919
TseI GCWGC 1 cut(s) 518
Tsp45I GTSAC 3 cut(s) 110, 274, 919
TspDTI ATGAA 4 cut(s) 37, 164, 236, 453
TspGWI ACGGA 1 cut(s) 342
TspRI CASTG 3 cut(s) 109, 283, 327
Tth111I GACNNNGTC 1 cut(s) 218
XapI RAATTY 3 cut(s) 347, 653, 789
XmiI GTMKAC 1 cut(s) 905
XmnI GAANNNNTTC 3 cut(s) 171, 959, 962
XspI CTAG 3 cut(s) 152, 713, 735
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.