MD07G1020700.v1.1

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0000539
Physical Location & Seq
Reverse (-)
1758750 .. 1760626
1877 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1020700.v1.1.491

Sequence Viewer

Length: 579 bp
ATGACAGCTCTTACCTATTTTTATCTTTTGTTCAAAAATGTCGAGGGTGGGATTCCGAGCTCCATAGGGATACTTTGCAACTTGAAGTTTTTCCGTTACACAAGCTGCATTTCTAGGAGTCCACTGGTTCTAACATCCTCCATCGTATCAATTGTATTTACTGGAAAACCTCTTTCTGCTGATGCAGTAGTCGATGAATCTTGGTTTTCAGATCCAGCTTTCTGTGAAAAATCAAAGCTTTGGTATCCGCTTTCAAAAACCTCAGCTGAGGAAGCTGCTTGGAAGTTTGCAGAGGAGAATGGAATCAATTTGGTTGCAGTAAATCCAGGAATGAATCCTTCAGCTAGTGGGAGGTATTCTATAGTTGGAAGCGTAGCACACTGTTCTGTGGCTGTCAAAACTTTCCGTGATCTCTTCCCTGCTCTCAAACTTCCCGAAAAATGTAAAGATGATCGACCTTTCACGCCAACTTACCAGATATCCAAGGAAAGAGCTCAGACTTTCGATGTTGAGTTTACTCCTCGGGAGGTGTCTTTGAAGGACACCGCTGAAGGTTTGAAGGAGAAGAACTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

21.35

Weight (kDa)

7.56

Isoelectric Point (pI)

25.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 248, 546
AclWI GGATC 1 cut(s) 206
AcuI CTGAAG 2 cut(s) 324, 570
AgsI TTSAA 5 cut(s) 34, 85, 255, 538, 559
AjnI CCWGG 1 cut(s) 325
AluBI AGCT 9 cut(s) 8, 60, 105, 218, 238, 266, 275, 344, 494
AluI AGCT 9 cut(s) 8, 60, 105, 218, 238, 266, 275, 344, 494
Alw21I GWGCWC 2 cut(s) 62, 496
AlwI GGATC 1 cut(s) 206
Ama87I CYCGRG 1 cut(s) 522
ApeKI GCWGC 2 cut(s) 105, 275
Asp700I GAANNNNTTC 3 cut(s) 89, 569, 572
AvaI CYCGRG 1 cut(s) 522
BanII GRGCYC 2 cut(s) 62, 496
Bbv12I GWGCWC 2 cut(s) 62, 496
BbvCI CCTCAGC 2 cut(s) 262, 267
BbvI GCAGC 2 cut(s) 92, 262
BccI CCATC 1 cut(s) 149
BciT130I CCWGG 1 cut(s) 327
BciVI GTATCC 2 cut(s) 63, 255
BfaI CTAG 2 cut(s) 114, 345
BfmI CTRYAG 1 cut(s) 360
BfuI GTATCC 2 cut(s) 63, 255
BisI GCNGC 2 cut(s) 106, 276
BlsI GCNGC 2 cut(s) 107, 277
Bme1390I CCNGG 1 cut(s) 327
BmeT110I CYCGRG 1 cut(s) 522
BmrFI CCNGG 1 cut(s) 327
BmsI GCATC 1 cut(s) 172
Bpu10I CCTNAGC 2 cut(s) 262, 267
BsaJI CCNNGG 2 cut(s) 483, 521
Bse1I ACTGG 2 cut(s) 129, 166
BseBI CCWGG 1 cut(s) 327
BseDI CCNNGG 2 cut(s) 483, 521
BseGI GGATG 1 cut(s) 134
BseMII CTCAG 3 cut(s) 258, 276, 509
BseNI ACTGG 2 cut(s) 129, 166
BseRI GAGGAG 2 cut(s) 308, 510
BseXI GCAGC 2 cut(s) 92, 262
BsiHKAI GWGCWC 2 cut(s) 62, 496
BsiHKCI CYCGRG 1 cut(s) 522
BsoBI CYCGRG 1 cut(s) 522
Bsp1286I GDGCHC 2 cut(s) 62, 496
Bsp143I GATC 3 cut(s) 211, 409, 451
BspACI CCGC 2 cut(s) 248, 546
BspCNI CTCAG 3 cut(s) 259, 275, 508
BspPI GGATC 1 cut(s) 206
BsrI ACTGG 2 cut(s) 129, 166
BssECI CCNNGG 2 cut(s) 483, 521
BssMI GATC 3 cut(s) 211, 409, 451
BssT1I CCWWGG 1 cut(s) 483
Bst2UI CCWGG 1 cut(s) 327
Bst4CI ACNGT 1 cut(s) 383
Bst6I CTCTTC 1 cut(s) 419
BstDEI CTNAG 3 cut(s) 262, 267, 495
BstF5I GGATG 1 cut(s) 134
BstKTI GATC 3 cut(s) 214, 412, 454
BstMBI GATC 3 cut(s) 211, 409, 451
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNI CCWGG 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 325
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 2 cut(s) 92, 262
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuI GTATCC 2 cut(s) 63, 255
BtsCI GGATG 1 cut(s) 134
BtsIMutI CAGTG 2 cut(s) 122, 379
DdeI CTNAG 3 cut(s) 262, 267, 495
DpnI GATC 3 cut(s) 213, 411, 453
DpnII GATC 3 cut(s) 211, 409, 451
Eam1104I CTCTTC 1 cut(s) 419
EarI CTCTTC 1 cut(s) 419
Ecl136II GAGCTC 2 cut(s) 60, 494
Eco130I CCWWGG 1 cut(s) 483
Eco24I GRGCYC 2 cut(s) 62, 496
Eco32I GATATC 1 cut(s) 480
Eco53kI GAGCTC 2 cut(s) 60, 494
Eco57I CTGAAG 2 cut(s) 324, 570
Eco88I CYCGRG 1 cut(s) 522
EcoICRI GAGCTC 2 cut(s) 60, 494
EcoRII CCWGG 1 cut(s) 325
EcoRV GATATC 1 cut(s) 480
EcoT14I CCWWGG 1 cut(s) 483
EcoT38I GRGCYC 2 cut(s) 62, 496
ErhI CCWWGG 1 cut(s) 483
FaiI YATR 2 cut(s) 65, 362
FalI AAGNNNNNCTT 1 cut(s) 557
Fnu4HI GCNGC 2 cut(s) 106, 276
FokI GGATG 1 cut(s) 121
FriOI GRGCYC 2 cut(s) 62, 496
Fsp4HI GCNGC 2 cut(s) 106, 276
FspBI CTAG 2 cut(s) 114, 345
GluI GCNGC 2 cut(s) 106, 276
HindIII AAGCTT 1 cut(s) 236
HinfI GANTC 5 cut(s) 52, 118, 197, 303, 334
Hpy166II GTNNAC 2 cut(s) 122, 516
Hpy188I TCNGA 4 cut(s) 57, 211, 498, 578
Hpy188III TCNNGA 2 cut(s) 434, 524
Hpy8I GTNNAC 2 cut(s) 122, 516
HpyAV CCTTC 4 cut(s) 348, 532, 545, 553
HpyCH4III ACNGT 1 cut(s) 383
HpyCH4V TGCA 5 cut(s) 78, 108, 185, 290, 317
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 3 cut(s) 262, 267, 495
Kzo9I GATC 3 cut(s) 211, 409, 451
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 7 cut(s) 110, 147, 228, 312, 339, 432, 488
Lsp1109I GCAGC 2 cut(s) 92, 262
LweI GCATC 1 cut(s) 172
MaeI CTAG 2 cut(s) 114, 345
MaeIII GTNAC 1 cut(s) 95
MalI GATC 3 cut(s) 213, 411, 453
MboI GATC 3 cut(s) 211, 409, 451
MboII GAAGA 3 cut(s) 406, 565, 577
MfeI CAATTG 1 cut(s) 150
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 2 cut(s) 62, 496
MluCI AATT 2 cut(s) 150, 307
MlyI GAGTC 1 cut(s) 127
MmeI TCCRAC 1 cut(s) 346
MnlI CCTC 9 cut(s) 37, 148, 180, 262, 271, 286, 345, 520, 531
MroXI GAANNNNTTC 3 cut(s) 89, 569, 572
MspA1I CMGCKG 2 cut(s) 266, 548
MspR9I CCNGG 1 cut(s) 327
MunI CAATTG 1 cut(s) 150
MvaI CCWGG 1 cut(s) 327
MwoI GCNNNNNNNGC 1 cut(s) 272
NdeII GATC 3 cut(s) 211, 409, 451
PdmI GAANNNNTTC 3 cut(s) 89, 569, 572
PfeI GAWTC 4 cut(s) 52, 197, 303, 334
PfoI TCCNGGA 1 cut(s) 325
PkrI GCNGC 2 cut(s) 107, 277
PleI GAGTC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 126
Psp124BI GAGCTC 2 cut(s) 62, 496
Psp6I CCWGG 1 cut(s) 325
PspGI CCWGG 1 cut(s) 325
PsuI RGATCY 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 266
SacI GAGCTC 2 cut(s) 62, 496
SatI GCNGC 2 cut(s) 106, 276
Sau3AI GATC 3 cut(s) 211, 409, 451
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 1 cut(s) 327
SduI GDGCHC 2 cut(s) 62, 496
SfaNI GCATC 1 cut(s) 172
SfcI CTRYAG 1 cut(s) 360
Sse9I AATT 2 cut(s) 150, 307
SsiI CCGC 2 cut(s) 248, 546
SspMI CTAG 2 cut(s) 114, 345
SstI GAGCTC 2 cut(s) 62, 496
StyD4I CCNGG 1 cut(s) 325
StyI CCWWGG 1 cut(s) 483
TaaI ACNGT 1 cut(s) 383
TaqI TCGA 4 cut(s) 42, 192, 454, 504
TasI AATT 2 cut(s) 150, 307
TfiI GAWTC 4 cut(s) 52, 197, 303, 334
TscAI CASTG 2 cut(s) 129, 386
TseI GCWGC 2 cut(s) 105, 275
TspDTI ATGAA 2 cut(s) 210, 347
TspGWI ACGGA 2 cut(s) 83, 395
TspRI CASTG 2 cut(s) 129, 386
XmnI GAANNNNTTC 3 cut(s) 89, 569, 572
XspI CTAG 2 cut(s) 114, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.