MD03G1172800.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000539
Physical Location & Seq
Forward (+)
23574621 .. 23580985
6365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1172800.v1.1.491

Sequence Viewer

Length: 978 bp
ATGAGCAGCGGAGAGGGGAAGGTCGTGTGCGTCACCGGTGCGTCCGGTTACATTGCTTCATGGCTCGTCAAGCTTCTTCTCCAGCGCGGCTACACCGTCAAGGCCTCCATTCGCGACCCAAATGATCCAACTAAGACAGAGCACTTGCATGCGCTTGATGGAGCTCAAGACAGACTTCAACTTTTCAAAGCAAATCTATTGGAAGAAGGTTCCTTTGACTCTGCTGTTGAGGGTTGTGAGGGCGTATTCCATACTGCATCCCCCTTTTATCATGATGTCACAGACCCAAAGGCAGAACTACTTGAACCTGCAGTAAAGGGAACCCTTAATGTTCTTAATTCGTGTGCGAAATCACCATCAATCAAACGGGTGGTTTTGACATCTTCTATAGCCGCAGTTGCATATAATGGAAAACCTCGAACGCCTGATGTAGTAATTGATGAGACTTGGTTTACAGATCCAGATGTTTGTAAGGAATCAAAGCTATGGTATGTGCTTTCAAAGACTTTGGCTGAGGATGCTGCCTGGAAATTTGTAAAGGAGAAGGGTATTGACTTGGTTACAATTAACCCTGCAATGGTGATAGGTCCTCTGTTACAGCCAACACTTAATACAAGTGCTGCAGCAGTTTTGAATGTTATTAAGGGAGCTCGAACATTTCCAAATGCAAGTTTTGGATGGATTAATGTTAAAGATGTTGCCAATGCACATATTCAAGCATTTGAGAGTCCTACCGCTAGTGGCAGATATTGTTTGGTGGAGACAGTTGCCCACTTCTCAGAAGTTGTGAGAATTTTACGTGAGCTGCACCCTACTTTGCAACTTCCAGAGAAGTGTGCGGACGACAAGCCTTTTGTGCCAACATATCAAGTCTCCAAGGAGAAGGCGAAAAGCTTGGGTGTCGAATTTATTCCACTAGACGTTAGCCTCAAGGAAACAGTTGAAAGCTTGAAGGAAAAAGGTTTTGTCAGTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

326

Amino Acids

35.45

Weight (kDa)

6.02

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 92 4.2e-09 NmrA-like family
Epimerase PF01370 9 - 246 1.2e-22 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 241 3.9e-16 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 11 - 213 7.5e-12 Male sterility protein
GDP_Man_Dehyd PF16363 11 - 256 1.5e-10 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 143 7.3e-11 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 316
AccII CGCG 2 cut(s) 87, 114
AciI CCGC 5 cut(s) 9, 87, 393, 735, 839
AclWI GGATC 2 cut(s) 119, 452
AcsI RAATTY 3 cut(s) 530, 792, 905
AfiI CCNNNNNNNGG 1 cut(s) 577
AgeI ACCGGT 1 cut(s) 35
AgsI TTSAA 8 cut(s) 179, 187, 305, 501, 634, 716, 944, 952
AjnI CCWGG 1 cut(s) 524
AluBI AGCT 7 cut(s) 73, 164, 484, 650, 805, 894, 948
AluI AGCT 7 cut(s) 73, 164, 484, 650, 805, 894, 948
Alw21I GWGCWC 3 cut(s) 144, 166, 652
Alw26I GTCTC 3 cut(s) 437, 755, 877
AlwI GGATC 2 cut(s) 119, 452
AlwNI CAGNNNCTG 1 cut(s) 975
AoxI GGCC 1 cut(s) 102
ApeKI GCWGC 5 cut(s) 6, 521, 620, 623, 805
ApoI RAATTY 3 cut(s) 530, 792, 905
ArsI GACNNNNNNTTYG 2 cut(s) 836, 868
AseI ATTAAT 1 cut(s) 684
AsiGI ACCGGT 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 909
AspLEI GCGC 2 cut(s) 87, 154
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 3 cut(s) 25, 345, 592
AvaII GGWCC 1 cut(s) 587
BanII GRGCYC 2 cut(s) 166, 652
Bbv12I GWGCWC 3 cut(s) 144, 166, 652
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 5 cut(s) 18, 508, 607, 635, 792
BccI CCATC 3 cut(s) 152, 364, 672
BciT130I CCWGG 1 cut(s) 526
BcoDI GTCTC 3 cut(s) 437, 755, 877
BfaI CTAG 2 cut(s) 738, 917
BfmI CTRYAG 3 cut(s) 309, 387, 621
BfuAI ACCTGC 1 cut(s) 316
BisI GCNGC 7 cut(s) 7, 88, 393, 522, 621, 624, 806
BlsI GCNGC 7 cut(s) 8, 89, 394, 523, 622, 625, 807
Bme1390I CCNGG 1 cut(s) 526
Bme18I GGWCC 1 cut(s) 587
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 2 cut(s) 211, 322
BmrFI CCNGG 1 cut(s) 526
BmsI GCATC 2 cut(s) 266, 508
BpmI CTGGAG 1 cut(s) 65
Bpu10I CCTNAGC 1 cut(s) 513
BpuEI CTTGAG 2 cut(s) 150, 914
BsaAI YACGTR 1 cut(s) 800
BsaJI CCNNGG 1 cut(s) 876
BsaWI WCCGGW 2 cut(s) 35, 44
Bsc4I CCNNNNNNNGG 1 cut(s) 577
Bse118I RCCGGY 1 cut(s) 35
Bse3DI GCAATG 2 cut(s) 51, 582
BseBI CCWGG 1 cut(s) 526
BseDI CCNNGG 1 cut(s) 876
BseGI GGATG 3 cut(s) 257, 523, 683
BseLI CCNNNNNNNGG 1 cut(s) 577
BseMI GCAATG 2 cut(s) 51, 582
BseMII CTCAG 2 cut(s) 504, 792
BseXI GCAGC 5 cut(s) 18, 508, 607, 635, 792
BsgI GTGCAG 1 cut(s) 791
Bsh1236I CGCG 2 cut(s) 87, 114
BshFI GGCC 1 cut(s) 104
BshTI ACCGGT 1 cut(s) 35
BsiHKAI GWGCWC 3 cut(s) 144, 166, 652
BsiSI CCGG 2 cut(s) 36, 45
BslI CCNNNNNNNGG 1 cut(s) 577
BsmAI GTCTC 3 cut(s) 437, 755, 877
BsnI GGCC 1 cut(s) 104
Bsp1286I GDGCHC 3 cut(s) 144, 166, 652
Bsp143I GATC 2 cut(s) 124, 457
Bsp68I TCGCGA 1 cut(s) 114
BspACI CCGC 5 cut(s) 9, 87, 393, 735, 839
BspANI GGCC 1 cut(s) 104
BspCNI CTCAG 2 cut(s) 505, 791
BspFNI CGCG 2 cut(s) 87, 114
BspHI TCATGA 1 cut(s) 271
BspLI GGNNCC 2 cut(s) 211, 322
BspMAI CTGCAG 2 cut(s) 313, 625
BspMI ACCTGC 1 cut(s) 316
BspPI GGATC 2 cut(s) 119, 452
BsrDI GCAATG 2 cut(s) 51, 582
BsrFI RCCGGY 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 1 cut(s) 876
BssMI GATC 2 cut(s) 124, 457
BssT1I CCWWGG 1 cut(s) 876
Bst2UI CCWGG 1 cut(s) 526
Bst4CI ACNGT 3 cut(s) 97, 766, 940
BstBAI YACGTR 1 cut(s) 800
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 3 cut(s) 132, 513, 778
BstF5I GGATG 3 cut(s) 257, 523, 683
BstFNI CGCG 2 cut(s) 87, 114
BstHHI GCGC 2 cut(s) 87, 154
BstKTI GATC 2 cut(s) 127, 460
BstMAI GTCTC 3 cut(s) 437, 755, 877
BstMBI GATC 2 cut(s) 124, 457
BstMWI GCNNNNNNNGC 4 cut(s) 70, 398, 518, 856
BstNI CCWGG 1 cut(s) 526
BstNSI RCATGY 1 cut(s) 152
BstSCI CCNGG 1 cut(s) 524
BstSFI CTRYAG 3 cut(s) 309, 387, 621
BstUI CGCG 2 cut(s) 87, 114
BstV1I GCAGC 5 cut(s) 18, 508, 607, 635, 792
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
BsuRI GGCC 1 cut(s) 104
BtsCI GGATG 3 cut(s) 257, 523, 683
BtuMI TCGCGA 1 cut(s) 114
BveI ACCTGC 1 cut(s) 316
Cac8I GCNNGC 1 cut(s) 150
CaiI CAGNNNCTG 1 cut(s) 975
CciI TCATGA 1 cut(s) 271
CfoI GCGC 2 cut(s) 87, 154
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 1 cut(s) 587
CseI GACGC 2 cut(s) 19, 30
CspAI ACCGGT 1 cut(s) 35
CviAII CATG 3 cut(s) 60, 149, 272
DdeI CTNAG 3 cut(s) 132, 513, 778
DpnI GATC 2 cut(s) 126, 459
DpnII GATC 2 cut(s) 124, 457
Ecl136II GAGCTC 2 cut(s) 164, 650
Eco130I CCWWGG 1 cut(s) 876
Eco147I AGGCCT 1 cut(s) 104
Eco24I GRGCYC 2 cut(s) 166, 652
Eco47I GGWCC 1 cut(s) 587
Eco53kI GAGCTC 2 cut(s) 164, 650
EcoICRI GAGCTC 2 cut(s) 164, 650
EcoO109I RGGNCCY 1 cut(s) 587
EcoRII CCWGG 1 cut(s) 524
EcoT14I CCWWGG 1 cut(s) 876
EcoT38I GRGCYC 2 cut(s) 166, 652
ErhI CCWWGG 1 cut(s) 876
FaeI CATG 3 cut(s) 63, 152, 275
FalI AAGNNNNNCTT 2 cut(s) 159, 191
FatI CATG 3 cut(s) 59, 148, 271
Fnu4HI GCNGC 7 cut(s) 7, 88, 393, 522, 621, 624, 806
FokI GGATG 3 cut(s) 244, 530, 690
FriOI GRGCYC 2 cut(s) 166, 652
Fsp4HI GCNGC 7 cut(s) 7, 88, 393, 522, 621, 624, 806
FspBI CTAG 2 cut(s) 738, 917
GlaI GCGC 2 cut(s) 86, 153
GluI GCNGC 7 cut(s) 7, 88, 393, 522, 621, 624, 806
GsuI CTGGAG 1 cut(s) 65
HaeIII GGCC 1 cut(s) 104
HapII CCGG 2 cut(s) 36, 45
HgaI GACGC 2 cut(s) 19, 30
HhaI GCGC 2 cut(s) 87, 154
Hin1II CATG 3 cut(s) 63, 152, 275
Hin6I GCGC 2 cut(s) 85, 152
HinP1I GCGC 2 cut(s) 85, 152
HindIII AAGCTT 3 cut(s) 71, 892, 946
HinfI GANTC 3 cut(s) 218, 476, 727
HpaII CCGG 2 cut(s) 36, 45
HphI GGTGA 3 cut(s) 25, 345, 592
Hpy166II GTNNAC 1 cut(s) 453
Hpy188I TCNGA 2 cut(s) 781, 977
Hpy188III TCNNGA 5 cut(s) 113, 167, 272, 461, 827
Hpy8I GTNNAC 1 cut(s) 453
HpyAV CCTTC 5 cut(s) 13, 200, 538, 877, 946
HpyCH4III ACNGT 3 cut(s) 97, 766, 940
HpyCH4IV ACGT 2 cut(s) 799, 921
HpyF10VI GCNNNNNNNGC 4 cut(s) 70, 398, 518, 856
HpyF3I CTNAG 3 cut(s) 132, 513, 778
HpySE526I ACGT 2 cut(s) 799, 921
Hsp92II CATG 3 cut(s) 63, 152, 275
HspAI GCGC 2 cut(s) 85, 152
Kzo9I GATC 2 cut(s) 124, 457
LmnI GCTCC 2 cut(s) 161, 647
Lsp1109I GCAGC 5 cut(s) 18, 508, 607, 635, 792
LweI GCATC 2 cut(s) 266, 508
MaeI CTAG 2 cut(s) 738, 917
MaeII ACGT 2 cut(s) 799, 921
MaeIII GTNAC 5 cut(s) 31, 47, 277, 559, 594
MalI GATC 2 cut(s) 126, 459
MboI GATC 2 cut(s) 124, 457
MboII GAAGA 3 cut(s) 68, 215, 375
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 3 cut(s) 144, 166, 652
MluCI AATT 6 cut(s) 337, 435, 530, 564, 792, 905
MlyI GAGTC 2 cut(s) 212, 736
MmeI TCCRAC 1 cut(s) 152
MnlI CCTC 8 cut(s) 7, 115, 223, 232, 426, 508, 600, 938
MroXI GAANNNNTTC 1 cut(s) 909
MseI TTAA 7 cut(s) 327, 336, 567, 609, 642, 684, 690
MslI CAYNNNNRTG 1 cut(s) 147
MspA1I CMGCKG 1 cut(s) 9
MspI CCGG 2 cut(s) 36, 45
MspR9I CCNGG 1 cut(s) 526
MvaI CCWGG 1 cut(s) 526
MvnI CGCG 2 cut(s) 87, 114
MwoI GCNNNNNNNGC 4 cut(s) 70, 398, 518, 856
NdeII GATC 2 cut(s) 124, 457
NlaIII CATG 3 cut(s) 63, 152, 275
NlaIV GGNNCC 2 cut(s) 211, 322
NmuCI GTSAC 2 cut(s) 31, 277
NruI TCGCGA 1 cut(s) 114
NspI RCATGY 1 cut(s) 152
PaeI GCATGC 1 cut(s) 152
PagI TCATGA 1 cut(s) 271
PceI AGGCCT 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 909
PfeI GAWTC 1 cut(s) 476
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 7 cut(s) 8, 89, 394, 523, 622, 625, 807
PleI GAGTC 2 cut(s) 212, 735
PpsI GAGTC 2 cut(s) 212, 735
Ppu21I YACGTR 1 cut(s) 800
PpuMI RGGWCCY 1 cut(s) 587
PshBI ATTAAT 1 cut(s) 684
Psp124BI GAGCTC 2 cut(s) 166, 652
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 524
PspGI CCWGG 1 cut(s) 524
PspN4I GGNNCC 2 cut(s) 211, 322
PspPI GGNCC 1 cut(s) 587
PspPPI RGGWCCY 1 cut(s) 587
PstI CTGCAG 2 cut(s) 313, 625
PstNI CAGNNNCTG 1 cut(s) 975
PsuI RGATCY 1 cut(s) 457
RruI TCGCGA 1 cut(s) 114
RseI CAYNNNNRTG 1 cut(s) 147
SacI GAGCTC 2 cut(s) 166, 652
SaqAI TTAA 7 cut(s) 327, 336, 567, 609, 642, 684, 690
SatI GCNGC 7 cut(s) 7, 88, 393, 522, 621, 624, 806
Sau3AI GATC 2 cut(s) 124, 457
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 2 cut(s) 212, 736
ScrFI CCNGG 1 cut(s) 526
SduI GDGCHC 3 cut(s) 144, 166, 652
SfaNI GCATC 2 cut(s) 266, 508
SfcI CTRYAG 3 cut(s) 309, 387, 621
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 1 cut(s) 587
SmiMI CAYNNNNRTG 1 cut(s) 147
SmlI CTYRAG 2 cut(s) 165, 929
SmoI CTYRAG 2 cut(s) 165, 929
SphI GCATGC 1 cut(s) 152
Sse9I AATT 6 cut(s) 337, 435, 530, 564, 792, 905
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 5 cut(s) 9, 87, 393, 735, 839
SspMI CTAG 2 cut(s) 738, 917
SstI GAGCTC 2 cut(s) 166, 652
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 1 cut(s) 524
StyI CCWWGG 1 cut(s) 876
TaaI ACNGT 3 cut(s) 97, 766, 940
TaiI ACGT 2 cut(s) 802, 924
TaqI TCGA 3 cut(s) 418, 652, 903
TasI AATT 6 cut(s) 337, 435, 530, 564, 792, 905
TauI GCSGC 2 cut(s) 90, 395
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 7 cut(s) 327, 336, 567, 609, 642, 684, 690
Tru9I TTAA 7 cut(s) 327, 336, 567, 609, 642, 684, 690
TseFI GTSAC 2 cut(s) 31, 277
TseI GCWGC 5 cut(s) 6, 521, 620, 623, 805
Tsp45I GTSAC 2 cut(s) 31, 277
TspDTI ATGAA 1 cut(s) 48
VpaK11BI GGWCC 1 cut(s) 587
VspI ATTAAT 1 cut(s) 684
XapI RAATTY 3 cut(s) 530, 792, 905
XceI RCATGY 1 cut(s) 152
XmnI GAANNNNTTC 1 cut(s) 909
XspI CTAG 2 cut(s) 738, 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.