RLG00000030591

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000539
Physical Location & Seq
Forward (+)
63974654 .. 63978016
3363 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030591

Sequence Viewer

Length: 780 bp
ATGAGCTGCGGAGAAAGCAAGGTTGTGTGTGTGACAGGAGCATCTGGGTTCATAGCATCGTGGCTAGTCAAGCTATTATTGCAACGAGGTTATACTGTCAAAGCCACTGTTCGGGACCCAAATGATCAAAAGAAAACAGAACACCTACTCTCACTTGATGGAGCAAAGGAAAGGCTTCATTTGTTCAAAGCAGACTTGTTAGCCGAAGGTTCTTTTGACCCCGTCGTTGATGGGTGTGAAGGTGTTTTTCATACAGCATCCCCTGCCCTACTCTCATCTACTAATCCGCAGCTTTGGTATATGCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAAAAAGGAATTGATATTATTACAATAAATCCGGGATGGGTGATCGGCCCTCTCTTACAGCCAACTCTGAACTTGAGTGTGGAACTAGTTCTGAAACTCGTAAATGGGACCGAAAAGTTTCCCAACAAAACTTACAGACTAGTTGATGTTAGAGATGTTGCTAATGCACATGTTCTAGCCTTTGAAAACCCATCAGCTACTGGACGTTATTGTTTAGTTGGAAGCGTAAAACACTGTTCAGAGGTTGTGAAAATGTTGTATGAGATCTCCCCTGCTCTCAATCTTCCAGATAAATGTGCAGATGACAAGCCCTTCACACCAACTTACCAAGTATCCAAGGAAAGAACCCAAACTTTGGGTGTAAAGTATACTCCGCTTGAAGTGTCTCTGAAGGATACTATTGAAAGTTTGAAGAACAAGAACTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

260

Amino Acids

28.6

Weight (kDa)

7.59

Isoelectric Point (pI)

24.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 86 1.4e-07 NmrA-like family
Epimerase PF01370 9 - 87 4.7e-09 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 11 - 84 5.5e-08 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 88 1.1e-07 NAD(P)H-binding
Epimerase PF01370 93 - 183 2.8e-06 NAD dependent epimerase/dehydratase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 703
AccI GTMKAC 1 cut(s) 716
AciI CCGC 3 cut(s) 9, 287, 722
AcuI CTGAAG 1 cut(s) 758
AfiI CCNNNNNNNGG 2 cut(s) 111, 703
AflIII ACRYGT 1 cut(s) 517
AgsI TTSAA 6 cut(s) 187, 309, 533, 728, 752, 760
AhlI ACTAGT 2 cut(s) 433, 487
AluBI AGCT 6 cut(s) 6, 73, 292, 320, 329, 545
AluI AGCT 6 cut(s) 6, 73, 292, 320, 329, 545
Alw26I GTCTC 1 cut(s) 738
AlwNI CAGNNNCTG 1 cut(s) 548
AoxI GGCC 1 cut(s) 394
ApeKI GCWGC 3 cut(s) 6, 289, 329
Asp700I GAANNNNTTC 4 cut(s) 174, 435, 465, 773
AspS9I GGNCC 3 cut(s) 115, 395, 456
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 1 cut(s) 400
AvaII GGWCC 2 cut(s) 115, 456
BbvCI CCTCAGC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 301, 316
BccI CCATC 4 cut(s) 152, 224, 378, 547
BciVI GTATCC 2 cut(s) 691, 736
BclI TGATCA 1 cut(s) 124
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 1 cut(s) 738
BcuI ACTAGT 2 cut(s) 433, 487
BfaI CTAG 4 cut(s) 65, 434, 488, 524
BfuI GTATCC 2 cut(s) 691, 736
BglII AGATCT 1 cut(s) 612
BisI GCNGC 3 cut(s) 7, 290, 330
BlsI GCNGC 3 cut(s) 8, 291, 331
Bme1390I CCNGG 1 cut(s) 381
Bme18I GGWCC 2 cut(s) 115, 456
BmgT120I GGNCC 3 cut(s) 115, 395, 456
BmiI GGNNCC 3 cut(s) 116, 117, 457
BmrFI CCNGG 1 cut(s) 381
BmsI GCATC 3 cut(s) 50, 65, 266
Bpu10I CCTNAGC 1 cut(s) 321
BpuEI CTTGAG 1 cut(s) 442
BpuMI CCSGG 1 cut(s) 381
BsaJI CCNNGG 1 cut(s) 684
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 703
Bse1I ACTGG 1 cut(s) 553
BseDI CCNNGG 1 cut(s) 684
BseGI GGATG 2 cut(s) 257, 389
BseLI CCNNNNNNNGG 2 cut(s) 111, 703
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 553
BseXI GCAGC 2 cut(s) 301, 316
BsgI GTGCAG 1 cut(s) 666
BshFI GGCC 1 cut(s) 396
BsiSI CCGG 1 cut(s) 380
BslFI GGGAC 2 cut(s) 128, 469
BslI CCNNNNNNNGG 2 cut(s) 111, 703
BsmAI GTCTC 1 cut(s) 738
BsmFI GGGAC 2 cut(s) 128, 469
BsnI GGCC 1 cut(s) 396
Bsp143I GATC 3 cut(s) 124, 390, 612
BspACI CCGC 3 cut(s) 9, 287, 722
BspANI GGCC 1 cut(s) 396
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 3 cut(s) 116, 117, 457
BsrI ACTGG 1 cut(s) 553
BssECI CCNNGG 1 cut(s) 684
BssMI GATC 3 cut(s) 124, 390, 612
BssNAI GTATAC 1 cut(s) 717
BssT1I CCWWGG 1 cut(s) 684
Bst1107I GTATAC 1 cut(s) 717
Bst4CI ACNGT 3 cut(s) 97, 109, 584
BstAPI GCANNNNNTGC 1 cut(s) 263
BstDEI CTNAG 1 cut(s) 321
BstF5I GGATG 2 cut(s) 257, 389
BstKTI GATC 3 cut(s) 127, 393, 615
BstMAI GTCTC 1 cut(s) 738
BstMBI GATC 3 cut(s) 124, 390, 612
BstMWI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 263, 326
BstNSI RCATGY 1 cut(s) 521
BstSCI CCNGG 1 cut(s) 379
BstV1I GCAGC 2 cut(s) 301, 316
BstX2I RGATCY 1 cut(s) 612
BstYI RGATCY 1 cut(s) 612
BstZ17I GTATAC 1 cut(s) 717
BsuI GTATCC 2 cut(s) 691, 736
BsuRI GGCC 1 cut(s) 396
BtsCI GGATG 2 cut(s) 257, 389
BtsIMutI CAGTG 2 cut(s) 105, 580
CaiI CAGNNNCTG 1 cut(s) 548
Cfr13I GGNCC 3 cut(s) 115, 395, 456
CviAII CATG 1 cut(s) 518
DdeI CTNAG 1 cut(s) 321
DpnI GATC 3 cut(s) 126, 392, 614
DpnII GATC 3 cut(s) 124, 390, 612
Eco130I CCWWGG 1 cut(s) 684
Eco47I GGWCC 2 cut(s) 115, 456
Eco57I CTGAAG 1 cut(s) 758
EcoO109I RGGNCCY 1 cut(s) 115
EcoT14I CCWWGG 1 cut(s) 684
ErhI CCWWGG 1 cut(s) 684
FaeI CATG 1 cut(s) 521
FaiI YATR 8 cut(s) 53, 93, 252, 300, 302, 519, 609, 717
FalI AAGNNNNNCTT 1 cut(s) 758
FaqI GGGAC 2 cut(s) 128, 469
FatI CATG 1 cut(s) 517
FbaI TGATCA 1 cut(s) 124
FblI GTMKAC 1 cut(s) 716
Fnu4HI GCNGC 3 cut(s) 7, 290, 330
FokI GGATG 2 cut(s) 244, 396
Fsp4HI GCNGC 3 cut(s) 7, 290, 330
FspBI CTAG 4 cut(s) 65, 434, 488, 524
GluI GCNGC 3 cut(s) 7, 290, 330
HaeIII GGCC 1 cut(s) 396
HapII CCGG 1 cut(s) 380
Hin1II CATG 1 cut(s) 521
HpaII CCGG 1 cut(s) 380
HphI GGTGA 1 cut(s) 400
Hpy166II GTNNAC 1 cut(s) 717
Hpy188I TCNGA 4 cut(s) 417, 441, 589, 738
Hpy188III TCNNGA 2 cut(s) 113, 635
Hpy8I GTNNAC 1 cut(s) 717
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 4 cut(s) 200, 233, 670, 733
HpyCH4III ACNGT 3 cut(s) 97, 109, 584
HpyCH4IV ACGT 1 cut(s) 553
HpyCH4V TGCA 4 cut(s) 82, 344, 515, 647
HpyF10VI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 263, 326
HpyF3I CTNAG 1 cut(s) 321
HpySE526I ACGT 1 cut(s) 553
Hsp92II CATG 1 cut(s) 521
KflI GGGWCCC 1 cut(s) 115
Ksp22I TGATCA 1 cut(s) 124
Kzo9I GATC 3 cut(s) 124, 390, 612
LmnI GCTCC 2 cut(s) 38, 161
LpnPI CCDG 7 cut(s) 21, 30, 276, 393, 534, 633, 648
Lsp1109I GCAGC 2 cut(s) 301, 316
LweI GCATC 3 cut(s) 50, 65, 266
MaeI CTAG 4 cut(s) 65, 434, 488, 524
MaeII ACGT 1 cut(s) 553
MaeIII GTNAC 1 cut(s) 31
MalI GATC 3 cut(s) 126, 392, 614
MboI GATC 3 cut(s) 124, 390, 612
MboII GAAGA 3 cut(s) 623, 766, 772
MflI RGATCY 1 cut(s) 612
MluCI AATT 1 cut(s) 357
MmeI TCCRAC 1 cut(s) 547
MnlI CCTC 4 cut(s) 80, 316, 408, 583
MroXI GAANNNNTTC 4 cut(s) 174, 435, 465, 773
MspI CCGG 1 cut(s) 380
MspR9I CCNGG 1 cut(s) 381
MwoI GCNNNNNNNGC 5 cut(s) 15, 70, 79, 263, 326
NciI CCSGG 1 cut(s) 381
NdeII GATC 3 cut(s) 124, 390, 612
NlaIII CATG 1 cut(s) 521
NlaIV GGNNCC 3 cut(s) 116, 117, 457
NmuCI GTSAC 1 cut(s) 31
NspI RCATGY 1 cut(s) 521
PciI ACATGT 1 cut(s) 517
PdmI GAANNNNTTC 4 cut(s) 174, 435, 465, 773
PflFI GACNNNGTC 1 cut(s) 221
PflMI CCANNNNNTGG 1 cut(s) 703
PfoI TCCNGGA 1 cut(s) 379
PkrI GCNGC 3 cut(s) 8, 291, 331
PpuMI RGGWCCY 1 cut(s) 115
PscI ACATGT 1 cut(s) 517
Psp5II RGGWCCY 1 cut(s) 115
PspN4I GGNNCC 3 cut(s) 116, 117, 457
PspPI GGNCC 3 cut(s) 115, 395, 456
PspPPI RGGWCCY 1 cut(s) 115
PstNI CAGNNNCTG 1 cut(s) 548
PsuI RGATCY 1 cut(s) 612
PsyI GACNNNGTC 1 cut(s) 221
SatI GCNGC 3 cut(s) 7, 290, 330
Sau3AI GATC 3 cut(s) 124, 390, 612
Sau96I GGNCC 3 cut(s) 115, 395, 456
ScrFI CCNGG 1 cut(s) 381
SfaNI GCATC 3 cut(s) 50, 65, 266
SinI GGWCC 2 cut(s) 115, 456
SmlI CTYRAG 1 cut(s) 421
SmoI CTYRAG 1 cut(s) 421
SpeI ACTAGT 2 cut(s) 433, 487
Sse9I AATT 1 cut(s) 357
SsiI CCGC 3 cut(s) 9, 287, 722
SspMI CTAG 4 cut(s) 65, 434, 488, 524
StyD4I CCNGG 1 cut(s) 379
StyI CCWWGG 1 cut(s) 684
TaaI ACNGT 3 cut(s) 97, 109, 584
TaiI ACGT 1 cut(s) 556
TaqII GACCGA 1 cut(s) 473
TasI AATT 1 cut(s) 357
TscAI CASTG 2 cut(s) 112, 587
TseFI GTSAC 1 cut(s) 31
TseI GCWGC 3 cut(s) 6, 289, 329
Tsp45I GTSAC 1 cut(s) 31
TspDTI ATGAA 3 cut(s) 40, 167, 239
TspRI CASTG 2 cut(s) 112, 587
Tth111I GACNNNGTC 1 cut(s) 221
Van91I CCANNNNNTGG 1 cut(s) 703
VpaK11BI GGWCC 2 cut(s) 115, 456
XceI RCATGY 1 cut(s) 521
XmiI GTMKAC 1 cut(s) 716
XmnI GAANNNNTTC 4 cut(s) 174, 435, 465, 773
XspI CTAG 4 cut(s) 65, 434, 488, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.