Rh1BG025000

reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000539
Physical Location & Seq
Reverse (-)
3359262 .. 3362512
3251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG025000.1

Sequence Viewer

Length: 975 bp
ATGAGTGGAGCAGAGAAGGTGGTGGTGAGCGTCACCGGAGCATCCGGTTACATTGCTTCATGGCTGGTCAAACTGTTGCTGCAACGCGGCTACACTGTCAAGGCCTCTGTTCGTGACCCAAATGACAATAAGAAAACAGAACACTTGCTTGCATTAGATGGAGCCAAGGAAAGGTTTCAATTGTTCAAAGCAGACCTGCTCGAAGAAGGATCTTTTGATTCCTTAGTTGATGGTAGTGTAGCTGTTTTTCATACCGCATCCCCATTTTATCACAATCCCAATGATCCACAGGTAGAACTAATTGACCCTGCCTTGAAGGGAACACTTAATGTCCTCAGATCATGTGTTAAGGTTCCGTCTATCAAGAGGGTGGTTATAACTTCCTCTATGGCAGCAGTCGCGTTCAATGGGAAACCTGTCGCTCCTGATGTAATAATTGATGAATCTTGGTTTTCAGATCCAGCTTTCTGTGAAAAATCAAAGGCTTGGTATATGCTTTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAGAAAGGAATTGATATTGTTACGATAAATCCGGGATTGGTGATTGGCCCTCTCTTACAGCCAACTCTCAACACAAGTGTGGAGCCAATCCTGAAACTCATAAATGTTACTTGGACTGAAAAATTTCCAAACACAACTTACAGATTTGTTGATGTTAGAGATGTTGCCAATGCACATATTCTAGCCTTTGAGAATCCTTCAGCTAGTGGACGCTATTGTTTAGTTGGAAGTGTAACACACTGTTCAGAGATTGTGAAAATTCTGCGTCATATCTTCCCTGCTCTCAGTCTTCCAGAAAAGCCTGCAGATGACAAACCTTTCACACCAACTTACCAGGTATCCAAGGAAAGAGCACAAACTTTGGGTGTAAAGTATACTCCGCTAGAAGTGACTCTAAAGGATACTGTTGAAAGTTTGAAGGACAAGAACTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

324

Amino Acids

35.71

Weight (kDa)

7.01

Isoelectric Point (pI)

30.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 105 3e-10 NmrA-like family
Epimerase PF01370 10 - 249 2.7e-24 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 11 - 244 1.8e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 11 - 200 6.9e-11 Male sterility protein
GDP_Man_Dehyd PF16363 11 - 256 1.9e-09 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 13 - 133 1.3e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 377
Acc36I ACCTGC 1 cut(s) 204
AccI GTMKAC 1 cut(s) 914
AccII CGCG 2 cut(s) 87, 401
AciI CCGC 3 cut(s) 87, 255, 920
AclWI GGATC 3 cut(s) 217, 278, 452
AcsI RAATTY 2 cut(s) 662, 798
AcuI CTGAAG 1 cut(s) 723
AfiI CCNNNNNNNGG 1 cut(s) 171
AgsI TTSAA 7 cut(s) 179, 187, 316, 406, 501, 950, 958
AjnI CCWGG 1 cut(s) 873
AleI CACNNNNGTG 1 cut(s) 617
AluBI AGCT 5 cut(s) 242, 464, 512, 521, 743
AluI AGCT 5 cut(s) 242, 464, 512, 521, 743
Alw21I GWGCWC 1 cut(s) 895
AlwI GGATC 3 cut(s) 217, 278, 452
AoxI GGCC 2 cut(s) 102, 586
ApeKI GCWGC 3 cut(s) 79, 392, 521
ApoI RAATTY 2 cut(s) 662, 798
Asp700I GAANNNNTTC 2 cut(s) 174, 663
AspS9I GGNCC 1 cut(s) 587
AsuC2I CCSGG 1 cut(s) 573
AsuHPI GGTGA 3 cut(s) 25, 37, 592
BbsI GAAGAC 1 cut(s) 821
Bbv12I GWGCWC 1 cut(s) 895
BbvCI CCTCAGC 1 cut(s) 513
BbvI GCAGC 3 cut(s) 66, 404, 508
BccI CCATC 2 cut(s) 152, 224
BciT130I CCWGG 1 cut(s) 875
BciVI GTATCC 2 cut(s) 889, 934
BcnI CCSGG 1 cut(s) 573
BfaI CTAG 3 cut(s) 722, 744, 923
BfmI CTRYAG 1 cut(s) 843
BfuAI ACCTGC 1 cut(s) 204
BfuI GTATCC 2 cut(s) 889, 934
BisI GCNGC 4 cut(s) 80, 88, 393, 522
BlsI GCNGC 4 cut(s) 81, 89, 394, 523
Bme1390I CCNGG 2 cut(s) 573, 875
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 3 cut(s) 163, 354, 624
BmrFI CCNGG 2 cut(s) 573, 875
BmsI GCATC 2 cut(s) 50, 266
BpiI GAAGAC 1 cut(s) 821
Bpu10I CCTNAGC 1 cut(s) 513
BpuMI CCSGG 1 cut(s) 573
BsaJI CCNNGG 2 cut(s) 165, 882
BsaWI WCCGGW 2 cut(s) 35, 44
Bsc4I CCNNNNNNNGG 1 cut(s) 171
Bse3DI GCAATG 1 cut(s) 51
BseBI CCWGG 1 cut(s) 875
BseDI CCNNGG 2 cut(s) 165, 882
BseGI GGATG 2 cut(s) 41, 257
BseLI CCNNNNNNNGG 1 cut(s) 171
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 3 cut(s) 349, 504, 838
BseXI GCAGC 3 cut(s) 66, 404, 508
Bsh1236I CGCG 2 cut(s) 87, 401
BshFI GGCC 2 cut(s) 104, 588
BsiHKAI GWGCWC 1 cut(s) 895
BsiSI CCGG 3 cut(s) 36, 45, 572
BslI CCNNNNNNNGG 1 cut(s) 171
BsnI GGCC 2 cut(s) 104, 588
Bsp1286I GDGCHC 1 cut(s) 895
Bsp143I GATC 4 cut(s) 209, 283, 338, 457
BspACI CCGC 3 cut(s) 87, 255, 920
BspANI GGCC 2 cut(s) 104, 588
BspCNI CTCAG 3 cut(s) 348, 505, 837
BspFNI CGCG 2 cut(s) 87, 401
BspLI GGNNCC 3 cut(s) 163, 354, 624
BspMAI CTGCAG 1 cut(s) 847
BspMI ACCTGC 1 cut(s) 204
BspPI GGATC 3 cut(s) 217, 278, 452
BsrDI GCAATG 1 cut(s) 51
BssECI CCNNGG 2 cut(s) 165, 882
BssMI GATC 4 cut(s) 209, 283, 338, 457
BssNAI GTATAC 1 cut(s) 915
BssT1I CCWWGG 2 cut(s) 165, 882
Bst1107I GTATAC 1 cut(s) 915
Bst2UI CCWGG 1 cut(s) 875
Bst4CI ACNGT 4 cut(s) 75, 97, 782, 946
BstC8I GCNNGC 2 cut(s) 150, 843
BstDEI CTNAG 4 cut(s) 223, 335, 513, 824
BstF5I GGATG 2 cut(s) 41, 257
BstFNI CGCG 2 cut(s) 87, 401
BstKTI GATC 4 cut(s) 212, 286, 341, 460
BstMBI GATC 4 cut(s) 209, 283, 338, 457
BstMWI GCNNNNNNNGC 2 cut(s) 398, 518
BstNI CCWGG 1 cut(s) 875
BstSCI CCNGG 2 cut(s) 571, 873
BstSFI CTRYAG 1 cut(s) 843
BstUI CGCG 2 cut(s) 87, 401
BstV1I GCAGC 3 cut(s) 66, 404, 508
BstV2I GAAGAC 1 cut(s) 821
BstX2I RGATCY 2 cut(s) 209, 457
BstYI RGATCY 2 cut(s) 209, 457
BstZ17I GTATAC 1 cut(s) 915
BsuI GTATCC 2 cut(s) 889, 934
BsuRI GGCC 2 cut(s) 104, 588
BtsCI GGATG 2 cut(s) 41, 257
BtsIMutI CAGTG 2 cut(s) 93, 778
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 2 cut(s) 150, 843
Cfr13I GGNCC 1 cut(s) 587
CseI GACGC 3 cut(s) 19, 759, 794
CsiI ACCWGGT 1 cut(s) 873
CviAII CATG 2 cut(s) 60, 342
DdeI CTNAG 4 cut(s) 223, 335, 513, 824
DpnI GATC 4 cut(s) 211, 285, 340, 459
DpnII GATC 4 cut(s) 209, 283, 338, 457
Eco130I CCWWGG 2 cut(s) 165, 882
Eco147I AGGCCT 1 cut(s) 104
Eco57I CTGAAG 1 cut(s) 723
EcoRII CCWGG 1 cut(s) 873
EcoT14I CCWWGG 2 cut(s) 165, 882
ErhI CCWWGG 2 cut(s) 165, 882
FaeI CATG 2 cut(s) 63, 345
FatI CATG 2 cut(s) 59, 341
FblI GTMKAC 1 cut(s) 914
Fnu4HI GCNGC 4 cut(s) 80, 88, 393, 522
FokI GGATG 2 cut(s) 28, 244
Fsp4HI GCNGC 4 cut(s) 80, 88, 393, 522
FspBI CTAG 3 cut(s) 722, 744, 923
GluI GCNGC 4 cut(s) 80, 88, 393, 522
HaeIII GGCC 2 cut(s) 104, 588
HapII CCGG 3 cut(s) 36, 45, 572
HgaI GACGC 3 cut(s) 19, 759, 794
Hin1II CATG 2 cut(s) 63, 345
HinfI GANTC 4 cut(s) 218, 443, 733, 931
HpaII CCGG 3 cut(s) 36, 45, 572
HphI GGTGA 3 cut(s) 25, 37, 592
Hpy166II GTNNAC 2 cut(s) 749, 915
Hpy188I TCNGA 3 cut(s) 338, 457, 787
Hpy188III TCNNGA 5 cut(s) 113, 364, 425, 631, 833
Hpy8I GTNNAC 2 cut(s) 749, 915
HpyAV CCTTC 5 cut(s) 10, 200, 310, 747, 952
HpyCH4III ACNGT 4 cut(s) 75, 97, 782, 946
HpyCH4V TGCA 5 cut(s) 82, 152, 536, 713, 845
HpyF10VI GCNNNNNNNGC 2 cut(s) 398, 518
HpyF3I CTNAG 4 cut(s) 223, 335, 513, 824
Hsp92II CATG 2 cut(s) 63, 345
Kzo9I GATC 4 cut(s) 209, 283, 338, 457
LmnI GCTCC 5 cut(s) 8, 38, 161, 427, 622
Lsp1109I GCAGC 3 cut(s) 66, 404, 508
LweI GCATC 2 cut(s) 50, 266
MabI ACCWGGT 1 cut(s) 873
MaeI CTAG 3 cut(s) 722, 744, 923
MaeIII GTNAC 7 cut(s) 31, 47, 113, 559, 646, 772, 928
MalI GATC 4 cut(s) 211, 285, 340, 459
MboI GATC 4 cut(s) 209, 283, 338, 457
MboII GAAGA 3 cut(s) 215, 805, 821
MfeI CAATTG 1 cut(s) 179
MflI RGATCY 2 cut(s) 209, 457
MhlI GDGCHC 1 cut(s) 895
MluCI AATT 6 cut(s) 179, 300, 435, 549, 662, 798
MlyI GAGTC 1 cut(s) 925
MmeI TCCRAC 1 cut(s) 745
MnlI CCTC 6 cut(s) 115, 344, 360, 394, 508, 600
MroXI GAANNNNTTC 2 cut(s) 174, 663
MseI TTAA 2 cut(s) 327, 348
MslI CAYNNNNRTG 1 cut(s) 617
MspI CCGG 3 cut(s) 36, 45, 572
MspR9I CCNGG 2 cut(s) 573, 875
MunI CAATTG 1 cut(s) 179
MvaI CCWGG 1 cut(s) 875
MvnI CGCG 2 cut(s) 87, 401
MwoI GCNNNNNNNGC 2 cut(s) 398, 518
NciI CCSGG 1 cut(s) 573
NdeII GATC 4 cut(s) 209, 283, 338, 457
NlaIII CATG 2 cut(s) 63, 345
NlaIV GGNNCC 3 cut(s) 163, 354, 624
NmuCI GTSAC 3 cut(s) 31, 113, 928
OliI CACNNNNGTG 1 cut(s) 617
PceI AGGCCT 1 cut(s) 104
PdmI GAANNNNTTC 2 cut(s) 174, 663
PfeI GAWTC 3 cut(s) 218, 443, 733
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 4 cut(s) 81, 89, 394, 523
PleI GAGTC 1 cut(s) 925
PpsI GAGTC 1 cut(s) 925
PsiI TTATAA 1 cut(s) 377
Psp6I CCWGG 1 cut(s) 873
PspGI CCWGG 1 cut(s) 873
PspN4I GGNNCC 3 cut(s) 163, 354, 624
PspPI GGNCC 1 cut(s) 587
PstI CTGCAG 1 cut(s) 847
PsuI RGATCY 2 cut(s) 209, 457
RseI CAYNNNNRTG 1 cut(s) 617
SaqAI TTAA 2 cut(s) 327, 348
SatI GCNGC 4 cut(s) 80, 88, 393, 522
Sau3AI GATC 4 cut(s) 209, 283, 338, 457
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 1 cut(s) 925
ScrFI CCNGG 2 cut(s) 573, 875
SduI GDGCHC 1 cut(s) 895
SexAI ACCWGGT 1 cut(s) 873
SfaNI GCATC 2 cut(s) 50, 266
SfcI CTRYAG 1 cut(s) 843
SmiMI CAYNNNNRTG 1 cut(s) 617
Sse9I AATT 6 cut(s) 179, 300, 435, 549, 662, 798
SseBI AGGCCT 1 cut(s) 104
SsiI CCGC 3 cut(s) 87, 255, 920
SspMI CTAG 3 cut(s) 722, 744, 923
StuI AGGCCT 1 cut(s) 104
StyD4I CCNGG 2 cut(s) 571, 873
StyI CCWWGG 2 cut(s) 165, 882
TaaI ACNGT 4 cut(s) 75, 97, 782, 946
TaqI TCGA 1 cut(s) 201
TasI AATT 6 cut(s) 179, 300, 435, 549, 662, 798
TauI GCSGC 1 cut(s) 90
TfiI GAWTC 3 cut(s) 218, 443, 733
Tru1I TTAA 2 cut(s) 327, 348
Tru9I TTAA 2 cut(s) 327, 348
TscAI CASTG 2 cut(s) 100, 785
TseFI GTSAC 3 cut(s) 31, 113, 928
TseI GCWGC 3 cut(s) 79, 392, 521
Tsp45I GTSAC 3 cut(s) 31, 113, 928
TspDTI ATGAA 3 cut(s) 48, 239, 456
TspGWI ACGGA 1 cut(s) 345
TspRI CASTG 2 cut(s) 100, 785
XapI RAATTY 2 cut(s) 662, 798
XmiI GTMKAC 1 cut(s) 914
XmnI GAANNNNTTC 2 cut(s) 174, 663
XspI CTAG 3 cut(s) 722, 744, 923
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.