Rorug01G0023900

NAD(P)H-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000539
Physical Location & Seq
Reverse (-)
3905691 .. 3912673
6983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0023900.1

Sequence Viewer

Length: 3906 bp
ATGGCTGAATTTCTCACTTTTGGTGCTCGAGAATTGTTGAGGAAAGTGGCTTCACTTGCTGCTCAAGAGTTTGATCTTGTATGGGGATTCAATGGAGAAATAACAAAGCTGCGTGACTGGGTGCCCATGCTTGAGGCTGTGCTACGAGATGCTGAACATCCGCGACAAGATCAGGGAGATGCTGTGAAGCTCTGGGTGAAGAAGCTTGAAGACATAGCTCAGCATGCCAATGATGTGCTGGATGATTATGGATATGAGCTTCTCCGGCGTAAGGTGCAACTGCGAAATCAGATGAAGAAAAAGGTGCAGAACTTTTTTTCCCTCTCCAATCCCATATTTTTTTGTGTTAAAATGGCACATAAAATTAAGAAAATCAACACATCTTTGGATGAGTTGAATAAGAAGGCAAGTACTATTGGCTTAGTTGCTAAACTATCCTTAGATGCAACAACTTCCCATGGTATAAGCATTGACAGGGAAACCTACTCTATGCAAGATGAAAACAATATCATTGGAAGGGACGATGTTGTGGCAGATATAGTTCAAGCCCTGACCAAATCAAACCACAATCTGGAAAGTGGTCTCTCAGTTCTGGCCATTGTGGGTATGGGAGGGCTTGGAAAGACAACTTTGGCTAAATCAATATATCATGAATCTGAGATAAGTAGGCACTTTGAGAAAAAGATGTGGATATGTGTATCTACCCTTTTTGAAGTCAATTCAATTTTGAGAGGGATCTTGGAATATCTTAAACCAGAAAATGCTGCAGTGAAAGCTATAGATGCAATATGTAATATTCTCCGAGAAGAGTTGAAAGGAAAGAGATACCTTCTTATACTTGATGATGTATGGAATGAAGATGCCGAAAAATGGAAACGTTTGAAGGATTGTTTGCTAGGAATTACCGATGCCCAAGGAAGCAGTATTATTGTCACTACTCGCAGTGATAAAGTTGCAAAAATGGTGGAGACCCTTCCTAGGTGTGATTTGAGAAAACTATCAGTTGATGAGTGTTGGCTTATATTGAAGAATAAAGCAATTCCAGTCGGAAGTGTTTCTATACTTGAAAATCAAGAGACAATTGGAAGGGAGATTGCCAAAAAGTGTGGAGGTGTACCATTAGTGGCAAAGGTATTAGGAAACATTATGCGCTCTAAAACGAGCAATGAATGGAGTTCAATTATGGAAAGTAAAATATGGGATTTGCCGGAAGAAGAAGAAAGAATCATGTCAATTTTAAAGTTGAGTTTTGATGAATTGAAATTTTCATCATTGAAACAATGTTTTGCATATTGCTCGGAATTCATCAAAGACTCTGTAATGGAAAAGGATGACTTAGTCCAACTTTGGATGGCTCAAGGATGGCTTCACCCCACCAAAAGTAATATGGAGATGGAGGATAGAGGGAACGAATATTTCAACATTTTATCAGAAAAATCCCTTTTTGAAGACGTTAGAACGGGTTATAATGGTAATACAACATGCAAGATGCACGATCTTGTGCATGACCTTGCAGAACGAGTGTCAAACATGACAGGCCGCTCACTATTTTTCAAGGGAGAAGCACTTGGGAACACCTTGCCTAACTTCAGATCTTTGCGTGTGTTAAATTTATATAAGGCAGACATTGACAAGTTGCCGAGTTCAATTGGAAAGTTGACACACTTGAGGTATCTGAATGTTATGAAAACAAATGTGAAAGCATTTCCAAAATCTATTGGTCAACTTTATTACCTTGAAACGTTTAAAATGCCTTATCATGTTGAAGAGTTCCCAAAGATGATTGCTGATATGATCAACTTGAGACATGTTTATTTTGGTGAATATACGAAAATTCCGCTTGGGATATTGGGAAGATTGACTAATCTCAGATCATTACCTTTTTTGAAGGTGGGTAAGGAAACTGGACCTAGAATTGAAGAACTGGGTGGGTTAAACCATTTGAGAGACACCTTAACTATCTATAATTTGGAGCATGTAACAGATGGTGAAGAAGCAGTGAAAGCAAAGTTAGTTGATAAGAAACATGTACGCAAGATAACTCTCCACTGGAAGCTTAGTAGACCAAGCGACAATGTTGACAATGAGGATGATGTACTTGAAGGCCTCCGACCACATTGTAATTTGGAAATTTTGAAGATTAGGGGGTTTATGGGTGTTAAGTTTCCATCATGGTTATTGCTTGCTAGCAACTTGAAAGAAATTGTATTAGCAGGGTGCAACAAATGCGAACGAGTTCCAATATTGGGCCATCTGCCCAATCTTATACATGTTAAGATGGAGGACATTCAGAACCTAAGATGCTTGGGGCCTGAGTTTTATGGTTACGATCAGATTTCCGGTGCAACAACAAGTGACGGGAGAAAGACTTTGTTTCCTGCTTTGAGATCATTACATATTAAGAATGCCGAGAATCTAATAGATTGGATGGAAGCAGGGGAAGTGATGCTGACGGCAGAAAAATTAAGGGTGTTTCCTTACCTTGAGGAGCTGACCCTGAAGCGGTGTAAGCAATTAAGAAGTGCTCCCAGTCATTTTCCATCTCTTAAGAAGTTGGAGATACGAGGAGTGGAAAGTGGTTTGCCTATAGCAAGTATTTTAAGCAATAAACTGACTACTCTCACTCATCTCTCGGTATATGATGTCAAGGGACTTACTAGTCTTCCAGAAGGGACGTTAAAAAACAACAAGAATCTTGAATCTTTGTATATAGGCGACTGTCCGGAGTTAAGTTGCATTGCTCCCCATGGATTTGACTGCTGCGCATCTCTTCAGGATCTGAGTATTTGGGAGTGTCCTAAGTTGAGGTATTTACCTGATGGGCTACTCCCACACTCTCTCAAGGAGTTGTCCATAATAGGTTGCTCCAGTCTAGAGTCCATCCCAATCATCCCTGAGCTAGGCGGTCTCCCATCTCTGCGCAAATTGTTTATATCCGGGTGTTCTCAGTTATCCAGTTTACCTGATGGGTTACAATACTGCACATCTCTTCAACTACTGGAGATACGGCGCTGCCCAAAGGTAACGTCCATTCCAGCCCAATACTGCACATCTCTTCAAGAATTGGAAATACACAGCTGCCCAAAGATAACGTCCATTCCAATTCCATCTGAGGGCCTCCCATCCCTTGGTCAACTTTGGCTTCGGTACTATCCTGAATTATCAAGCCTACCGAGTGGGCTAGGCTGCTGCCCCTATCTTGTTGATTTGTCAATAAGAGAGTGCCCTAAGGTAACATCCATTCCAATTTTACACGGAGGCTTCTCATCCCTCCGTCAATTGGTAATCGACGGTTGTCAAAGTACACAAATTGATCTGCAATTTTGTCAAATCCTCGTCTCTCTTGAGGAGTTGACAATAAGTGATTGTCCTAATCTAGAGACTGTTCCAAGTTTAGACAAGCTCACATCCCTCCGTAGATTGGTGATTTTTCGATGTTCCCGATTGACATGTCTACCCAGAGTGGCAATGGCATCCCCACACGTATTCACTCGTCTCAAGTCCTTGTCACTTGGTCCGTTTTGGAATGAGCTGGATTCATTCCCGGCTCTTCAGGTTCTACCACAACTTGAATCATTATGGATCTACGGGTGGCCGAAGCTCAAGTCTCTGCCTGAACAAATTCAACACTTGACGTCTTTGACCTCTTTGGGGATACTTGGCTTTGAGGGAGTGGAGGCTATTCCAGAATGGTTAGGAAACCTTGCATCTCTTCACTCCTTAGATATTATGTGTTGCGAGAATCTGATGTATCTACCTTCCGTCCAAGCTATGCAACGCCTCACCAAATTAGATTCGCTACGTATCTCCAACTGTCCCCTTTTGAGCGAAAGATGCACCGCGGAGAGCGGCCCAGAGTGGCCTAAGATTTCTCATCTTCCAGACATCTATGTATTATTAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

1301

Amino Acids

146.64

Weight (kDa)

6.79

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 97 1.4e-19 Rx N-terminal domain
NB-ARC PF00931 176 - 348 2.1e-35 NB-ARC domain
WHD_DRP PF23559 435 - 505 2.2e-21 Disease resistance protein Winged helix domain
LRR_14 PF23598 515 - 855 4.6e-30 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 639 - 762 1.5e-34 R13L1/DRL21 LRRs
LRR_14 PF23598 1048 - 1219 1.5e-07 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1089 - 1144 8.7e-06 R13L1/DRL21 LRRs
LRR_14 PF23598 1186 - 1271 7.3e-06 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 1206 - 1271 1.4e-06 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1467
AasI GACNNNNNNGTC 1 cut(s) 2658
AatII GACGTC 1 cut(s) 3638
Acc16I TGCGCA 2 cut(s) 2764, 2921
AccB1I GGYRCC 1 cut(s) 121
AccB7I CCANNNNNTGG 2 cut(s) 571, 3128
AccBSI CCGCTC 2 cut(s) 1542, 3849
AccI GTMKAC 2 cut(s) 2062, 3454
AccII CGCG 2 cut(s) 163, 3842
AccIII TCCGGA 1 cut(s) 2722
AciI CCGC 8 cut(s) 161, 1540, 1838, 2503, 2904, 3840, 3842, 3849
AclI AACGTT 2 cut(s) 877, 1742
AclWI GGATC 3 cut(s) 743, 2784, 3590
AcoI YGGCCR 2 cut(s) 594, 3593
AcsI RAATTY 7 cut(s) 8, 1262, 1301, 1609, 1833, 2130, 3621
AcuI CTGAAG 4 cut(s) 1573, 2519, 2756, 3536
AcyI GRCGYC 1 cut(s) 3635
AfaI GTAC 6 cut(s) 412, 1116, 2031, 2097, 3149, 3304
AflII CTTAAG 1 cut(s) 2546
AflIII ACRYGT 5 cut(s) 1807, 2026, 2269, 3449, 3481
AhlI ACTAGT 1 cut(s) 2657
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
Alw21I GWGCWC 2 cut(s) 28, 2527
AlwI GGATC 3 cut(s) 743, 2784, 3590
AlwNI CAGNNNCTG 1 cut(s) 2779
Ama87I CYCGRG 1 cut(s) 27
Aor13HI TCCGGA 1 cut(s) 2722
AoxI GGCC 9 cut(s) 594, 1537, 2104, 2248, 2309, 3115, 3593, 3850, 3860
ApeKI GCWGC 8 cut(s) 59, 109, 764, 2760, 3012, 3078, 3186, 3189
ApoI RAATTY 7 cut(s) 8, 1262, 1301, 1609, 1833, 2130, 3621
ArsI GACNNNNNNTTYG 6 cut(s) 3011, 3043, 3077, 3109, 3590, 3622
Asp700I GAANNNNTTC 3 cut(s) 1054, 2235, 3621
AspA2I CCTAGG 1 cut(s) 977
AspLEI GCGC 4 cut(s) 1152, 2765, 2922, 3012
AspS9I GGNCC 6 cut(s) 1907, 2248, 2309, 3115, 3515, 3851
AsuC2I CCSGG 2 cut(s) 2938, 3545
AsuHPI GGTGA 6 cut(s) 208, 1361, 1832, 2000, 3436, 3775
AsuNHI GCTAGC 1 cut(s) 2186
AvaI CYCGRG 1 cut(s) 27
AvaII GGWCC 2 cut(s) 1907, 3515
AvrII CCTAGG 1 cut(s) 977
AxyI CCTNAGG 1 cut(s) 3228
BaeGI GKGCMC 2 cut(s) 126, 3227
BalI TGGCCA 1 cut(s) 596
BanI GGYRCC 1 cut(s) 121
BbsI GAAGAC 3 cut(s) 216, 1455, 2654
Bbv12I GWGCWC 2 cut(s) 28, 2527
BbvI GCAGC 8 cut(s) 46, 96, 751, 2747, 2999, 3065, 3173, 3176
BceAI ACGGC 2 cut(s) 2469, 3023
BcgI CGANNNNNNTGC 2 cut(s) 1278, 1312
BciVI GTATCC 1 cut(s) 3648
BclI TGATCA 1 cut(s) 1794
BcnI CCSGG 2 cut(s) 2938, 3545
BcuI ACTAGT 1 cut(s) 2657
BfaI CTAG 9 cut(s) 896, 978, 1911, 2187, 2658, 2873, 2900, 3182, 3377
BfmI CTRYAG 3 cut(s) 765, 777, 2586
BfoI RGCGCY 1 cut(s) 3013
BfrI CTTAAG 1 cut(s) 2546
BfuI GTATCC 1 cut(s) 3648
BglII AGATCT 1 cut(s) 1592
BlnI CCTAGG 1 cut(s) 977
BlpI GCTNAGC 1 cut(s) 219
BmcAI AGTACT 1 cut(s) 412
Bme1390I CCNGG 2 cut(s) 2938, 3545
Bme18I GGWCC 2 cut(s) 1907, 3515
BmeT110I CYCGRG 1 cut(s) 27
BmgT120I GGNCC 6 cut(s) 1907, 2248, 2309, 3115, 3515, 3851
BmiI GGNNCC 2 cut(s) 123, 2310
BmrFI CCNGG 2 cut(s) 2938, 3545
BmrI ACTGGG 3 cut(s) 127, 1934, 2523
BmtI GCTAGC 1 cut(s) 2190
BmuI ACTGGG 3 cut(s) 127, 1934, 2523
BoxI GACNNNNGTC 1 cut(s) 3294
BpiI GAAGAC 3 cut(s) 216, 1455, 2654
BpmI CTGGAG 2 cut(s) 2851, 3020
Bpu10I CCTNAGC 1 cut(s) 2895
Bpu1102I GCTNAGC 1 cut(s) 219
BpuMI CCSGG 2 cut(s) 2938, 3545
BsaAI YACGTR 2 cut(s) 3484, 3803
BsaHI GRCGYC 1 cut(s) 3635
BsaI GGTCTC 3 cut(s) 587, 962, 2912
BsaJI CCNNGG 6 cut(s) 457, 913, 977, 2746, 3127, 3840
BsaWI WCCGGW 2 cut(s) 2339, 2722
BsaXI ACNNNNNCTCC 2 cut(s) 168, 198
Bse1I ACTGG 9 cut(s) 122, 1043, 1909, 1929, 2054, 2529, 2868, 2955, 3003
Bse21I CCTNAGG 1 cut(s) 3228
Bse3DI GCAATG 3 cut(s) 1171, 2736, 3474
BseAI TCCGGA 1 cut(s) 2722
BseDI CCNNGG 6 cut(s) 457, 913, 977, 2746, 3127, 3840
BseMI GCAATG 3 cut(s) 1171, 2736, 3474
BseMII CTCAG 9 cut(s) 233, 600, 648, 1882, 2304, 2771, 2886, 2960, 3102
BseNI ACTGG 9 cut(s) 122, 1043, 1909, 1929, 2054, 2529, 2868, 2955, 3003
BseRI GAGGAG 3 cut(s) 2501, 2580, 3362
BseSI GKGCMC 2 cut(s) 126, 3227
BseXI GCAGC 8 cut(s) 46, 96, 751, 2747, 2999, 3065, 3173, 3176
BsgI GTGCAG 3 cut(s) 326, 2965, 3031
Bsh1236I CGCG 2 cut(s) 163, 3842
BshFI GGCC 9 cut(s) 596, 1539, 2106, 2250, 2311, 3117, 3595, 3852, 3862
BshNI GGYRCC 1 cut(s) 121
BsiHKAI GWGCWC 2 cut(s) 28, 2527
BsiHKCI CYCGRG 1 cut(s) 27
BsiSI CCGG 6 cut(s) 265, 1208, 2340, 2723, 2937, 3545
BslFI GGGAC 4 cut(s) 533, 2664, 2686, 3801
BsmBI CGTCTC 2 cut(s) 3343, 3500
BsmFI GGGAC 4 cut(s) 533, 2664, 2686, 3801
BsmI GAATGC 1 cut(s) 2410
BsnI GGCC 9 cut(s) 596, 1539, 2106, 2250, 2311, 3117, 3595, 3852, 3862
Bso31I GGTCTC 3 cut(s) 587, 962, 2912
BsoBI CYCGRG 1 cut(s) 27
Bsp1286I GDGCHC 4 cut(s) 28, 126, 2527, 3227
Bsp13I TCCGGA 1 cut(s) 2722
Bsp1720I GCTNAGC 1 cut(s) 219
Bsp19I CCATGG 2 cut(s) 457, 2746
BspACI CCGC 8 cut(s) 161, 1540, 1838, 2503, 2904, 3840, 3842, 3849
BspANI GGCC 9 cut(s) 596, 1539, 2106, 2250, 2311, 3117, 3595, 3852, 3862
BspCNI CTCAG 9 cut(s) 232, 599, 649, 1881, 2305, 2772, 2887, 2959, 3103
BspEI TCCGGA 1 cut(s) 2722
BspFNI CGCG 2 cut(s) 163, 3842
BspHI TCATGA 1 cut(s) 649
BspLI GGNNCC 2 cut(s) 123, 2310
BspMAI CTGCAG 1 cut(s) 769
BspOI GCTAGC 1 cut(s) 2190
BspPI GGATC 3 cut(s) 743, 2784, 3590
BspQI GCTCTTC 1 cut(s) 3555
BspT107I GGYRCC 1 cut(s) 121
BspTI CTTAAG 1 cut(s) 2546
BspTNI GGTCTC 3 cut(s) 587, 962, 2912
BsrBI CCGCTC 2 cut(s) 1542, 3849
BsrDI GCAATG 3 cut(s) 1171, 2736, 3474
BsrI ACTGG 9 cut(s) 122, 1043, 1909, 1929, 2054, 2529, 2868, 2955, 3003
BssECI CCNNGG 6 cut(s) 457, 913, 977, 2746, 3127, 3840
BssNI GRCGYC 1 cut(s) 3635
BssT1I CCWWGG 5 cut(s) 457, 913, 977, 2746, 3127
Bst4CI ACNGT 4 cut(s) 2720, 3293, 3385, 3815
Bst6I CTCTTC 7 cut(s) 801, 1761, 2775, 2994, 3060, 3555, 3717
BstACI GRCGYC 1 cut(s) 3635
BstAFI CTTAAG 1 cut(s) 2546
BstAPI GCANNNNNTGC 1 cut(s) 2226
BstBAI YACGTR 2 cut(s) 3484, 3803
BstC8I GCNNGC 3 cut(s) 225, 2184, 2188
BstDSI CCRYGG 3 cut(s) 457, 2746, 3840
BstENI CCTNNNNNAGG 1 cut(s) 2898
BstFNI CGCG 2 cut(s) 163, 3842
BstH2I RGCGCY 1 cut(s) 3013
BstHHI GCGC 4 cut(s) 1152, 2765, 2922, 3012
BstNSI RCATGY 7 cut(s) 227, 1485, 1811, 1979, 2030, 2273, 3453
BstPAI GACNNNNGTC 1 cut(s) 3294
BstSCI CCNGG 2 cut(s) 2936, 3543
BstSFI CTRYAG 3 cut(s) 765, 777, 2586
BstSLI GKGCMC 2 cut(s) 126, 3227
BstSNI TACGTA 1 cut(s) 3803
BstUI CGCG 2 cut(s) 163, 3842
BstV1I GCAGC 8 cut(s) 46, 96, 751, 2747, 2999, 3065, 3173, 3176
BstV2I GAAGAC 3 cut(s) 216, 1455, 2654
BstX2I RGATCY 4 cut(s) 735, 1592, 2776, 3582
BstYI RGATCY 4 cut(s) 735, 1592, 2776, 3582
Bsu36I CCTNAGG 1 cut(s) 3228
BsuI GTATCC 1 cut(s) 3648
BsuRI GGCC 9 cut(s) 596, 1539, 2106, 2250, 2311, 3117, 3595, 3852, 3862
BtgI CCRYGG 3 cut(s) 457, 2746, 3840
BtsI GCAGTG 3 cut(s) 774, 949, 2004
BtsIMutI CAGTG 4 cut(s) 774, 949, 2004, 2047
Cac8I GCNNGC 3 cut(s) 225, 2184, 2188
CaiI CAGNNNCTG 1 cut(s) 2779
CciI TCATGA 1 cut(s) 649
CfoI GCGC 4 cut(s) 1152, 2765, 2922, 3012
Cfr13I GGNCC 6 cut(s) 1907, 2248, 2309, 3115, 3515, 3851
Cfr42I CCGCGG 1 cut(s) 3843
Csp6I GTAC 6 cut(s) 411, 1115, 2030, 2096, 3148, 3303
CspCI CAANNNNNGTGG 4 cut(s) 945, 980, 1087, 1122
CviQI GTAC 6 cut(s) 411, 1115, 2030, 2096, 3148, 3303
DraI TTTAAA 2 cut(s) 1239, 1747
DrdI GACNNNNNNGTC 1 cut(s) 2658
DseDI GACNNNNNNGTC 1 cut(s) 2658
EaeI YGGCCR 2 cut(s) 594, 3593
Eam1104I CTCTTC 7 cut(s) 801, 1761, 2775, 2994, 3060, 3555, 3717
EarI CTCTTC 7 cut(s) 801, 1761, 2775, 2994, 3060, 3555, 3717
Eco105I TACGTA 1 cut(s) 3803
Eco130I CCWWGG 5 cut(s) 457, 913, 977, 2746, 3127
Eco147I AGGCCT 1 cut(s) 2106
Eco31I GGTCTC 3 cut(s) 587, 962, 2912
Eco47I GGWCC 2 cut(s) 1907, 3515
Eco57I CTGAAG 4 cut(s) 1573, 2519, 2756, 3536
Eco81I CCTNAGG 1 cut(s) 3228
Eco88I CYCGRG 1 cut(s) 27
EcoNI CCTNNNNNAGG 1 cut(s) 2898
EcoO109I RGGNCCY 2 cut(s) 2309, 3115
EcoRI GAATTC 1 cut(s) 1301
EcoT14I CCWWGG 5 cut(s) 457, 913, 977, 2746, 3127
ErhI CCWWGG 5 cut(s) 457, 913, 977, 2746, 3127
Esp3I CGTCTC 2 cut(s) 3343, 3500
FalI AAGNNNNNCTT 4 cut(s) 1319, 1351, 1350, 1382
FaqI GGGAC 4 cut(s) 533, 2664, 2686, 3801
FbaI TGATCA 1 cut(s) 1794
FblI GTMKAC 2 cut(s) 2062, 3454
FspBI CTAG 9 cut(s) 896, 978, 1911, 2187, 2658, 2873, 2900, 3182, 3377
FspI TGCGCA 2 cut(s) 2764, 2921
GlaI GCGC 4 cut(s) 1151, 2764, 2921, 3011
GsuI CTGGAG 2 cut(s) 2851, 3020
HaeII RGCGCY 1 cut(s) 3013
HaeIII GGCC 9 cut(s) 596, 1539, 2106, 2250, 2311, 3117, 3595, 3852, 3862
HapII CCGG 6 cut(s) 265, 1208, 2340, 2723, 2937, 3545
HhaI GCGC 4 cut(s) 1152, 2765, 2922, 3012
Hin1I GRCGYC 1 cut(s) 3635
Hin6I GCGC 4 cut(s) 1150, 2763, 2920, 3010
HinP1I GCGC 4 cut(s) 1150, 2763, 2920, 3010
HincII GTYRAC 5 cut(s) 1659, 1724, 2080, 3134, 3354
HindII GTYRAC 5 cut(s) 1659, 1724, 2080, 3134, 3354
HindIII AAGCTT 2 cut(s) 203, 2054
HpaII CCGG 6 cut(s) 265, 1208, 2340, 2723, 2937, 3545
HphI GGTGA 6 cut(s) 208, 1361, 1832, 2000, 3436, 3775
Hpy99I CGWCG 1 cut(s) 3293
HpyCH4III ACNGT 4 cut(s) 2720, 3293, 3385, 3815
HpyCH4IV ACGT 9 cut(s) 877, 1452, 1742, 2675, 3026, 3092, 3483, 3635, 3802
HpySE526I ACGT 9 cut(s) 877, 1452, 1742, 2675, 3026, 3092, 3483, 3635, 3802
Hsp92I GRCGYC 1 cut(s) 3635
HspAI GCGC 4 cut(s) 1150, 2763, 2920, 3010
Kpn2I TCCGGA 1 cut(s) 2722
Ksp22I TGATCA 1 cut(s) 1794
KspI CCGCGG 1 cut(s) 3843
LguI GCTCTTC 1 cut(s) 3555
LmnI GCTCC 5 cut(s) 1972, 2488, 2530, 2746, 2870
Lsp1109I GCAGC 8 cut(s) 46, 96, 751, 2747, 2999, 3065, 3173, 3176
MaeI CTAG 9 cut(s) 896, 978, 1911, 2187, 2658, 2873, 2900, 3182, 3377
MaeII ACGT 9 cut(s) 877, 1452, 1742, 2675, 3026, 3092, 3483, 3635, 3802
MaeIII GTNAC 9 cut(s) 113, 931, 1978, 2324, 2354, 2970, 3022, 3232, 3507
MbiI CCGCTC 2 cut(s) 1542, 3849
MfeI CAATTG 3 cut(s) 1080, 1647, 3278
MflI RGATCY 4 cut(s) 735, 1592, 2776, 3582
MhlI GDGCHC 4 cut(s) 28, 126, 2527, 3227
MlsI TGGCCA 1 cut(s) 596
MluNI TGGCCA 1 cut(s) 596
MlyI GAGTC 2 cut(s) 1307, 2885
MmeI TCCRAC 5 cut(s) 1027, 1366, 2134, 2535, 3834
Mox20I TGGCCA 1 cut(s) 596
MroI TCCGGA 1 cut(s) 2722
MroXI GAANNNNTTC 3 cut(s) 1054, 2235, 3621
MscI TGGCCA 1 cut(s) 596
MslI CAYNNNNRTG 1 cut(s) 228
Msp20I TGGCCA 1 cut(s) 596
MspA1I CMGCKG 2 cut(s) 3078, 3842
MspCI CTTAAG 1 cut(s) 2546
MspI CCGG 6 cut(s) 265, 1208, 2340, 2723, 2937, 3545
MspR9I CCNGG 2 cut(s) 2938, 3545
MunI CAATTG 3 cut(s) 1080, 1647, 3278
Mva1269I GAATGC 1 cut(s) 2410
MvnI CGCG 2 cut(s) 163, 3842
NciI CCSGG 2 cut(s) 2938, 3545
NcoI CCATGG 2 cut(s) 457, 2746
NheI GCTAGC 1 cut(s) 2186
NlaIV GGNNCC 2 cut(s) 123, 2310
NmeAIII GCCGAG 2 cut(s) 1665, 2434
NmuCI GTSAC 4 cut(s) 113, 931, 2354, 3507
NsbI TGCGCA 2 cut(s) 2764, 2921
NspI RCATGY 7 cut(s) 227, 1485, 1811, 1979, 2030, 2273, 3453
PaeI GCATGC 1 cut(s) 227
PaeR7I CTCGAG 1 cut(s) 27
PagI TCATGA 1 cut(s) 649
PceI AGGCCT 1 cut(s) 2106
PciI ACATGT 4 cut(s) 1807, 2026, 2269, 3449
PciSI GCTCTTC 1 cut(s) 3555
PcsI WCGNNNNNNNCGW 1 cut(s) 3439
PctI GAATGC 1 cut(s) 2410
PdmI GAANNNNTTC 3 cut(s) 1054, 2235, 3621
PflFI GACNNNGTC 1 cut(s) 1337
PflMI CCANNNNNTGG 2 cut(s) 571, 3128
PleI GAGTC 2 cut(s) 1307, 2884
PpsI GAGTC 2 cut(s) 1307, 2884
Ppu21I YACGTR 2 cut(s) 3484, 3803
PscI ACATGT 4 cut(s) 1807, 2026, 2269, 3449
PshAI GACNNNNGTC 1 cut(s) 3294
PsiI TTATAA 1 cut(s) 1467
Psp1406I AACGTT 2 cut(s) 877, 1742
PspN4I GGNNCC 2 cut(s) 123, 2310
PspPI GGNCC 6 cut(s) 1907, 2248, 2309, 3115, 3515, 3851
PstI CTGCAG 1 cut(s) 769
PstNI CAGNNNCTG 1 cut(s) 2779
PsuI RGATCY 4 cut(s) 735, 1592, 2776, 3582
PsyI GACNNNGTC 1 cut(s) 1337
PvuII CAGCTG 1 cut(s) 3078
RsaI GTAC 6 cut(s) 412, 1116, 2031, 2097, 3149, 3304
RsaNI GTAC 6 cut(s) 411, 1115, 2030, 2096, 3148, 3303
RseI CAYNNNNRTG 1 cut(s) 228
SacII CCGCGG 1 cut(s) 3843
SapI GCTCTTC 1 cut(s) 3555
Sau96I GGNCC 6 cut(s) 1907, 2248, 2309, 3115, 3515, 3851
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 2 cut(s) 1307, 2885
ScrFI CCNGG 2 cut(s) 2938, 3545
SduI GDGCHC 4 cut(s) 28, 126, 2527, 3227
SfcI CTRYAG 3 cut(s) 765, 777, 2586
Sfr274I CTCGAG 1 cut(s) 27
Sfr303I CCGCGG 1 cut(s) 3843
SgrBI CCGCGG 1 cut(s) 3843
SinI GGWCC 2 cut(s) 1907, 3515
SlaI CTCGAG 1 cut(s) 27
SmiMI CAYNNNNRTG 1 cut(s) 228
SnaBI TACGTA 1 cut(s) 3803
SpeI ACTAGT 1 cut(s) 2657
SphI GCATGC 1 cut(s) 227
SseBI AGGCCT 1 cut(s) 2106
SsiI CCGC 8 cut(s) 161, 1540, 1838, 2503, 2904, 3840, 3842, 3849
SspI AATATT 3 cut(s) 796, 1415, 2244
SspMI CTAG 9 cut(s) 896, 978, 1911, 2187, 2658, 2873, 2900, 3182, 3377
StuI AGGCCT 1 cut(s) 2106
StyD4I CCNGG 2 cut(s) 2936, 3543
StyI CCWWGG 5 cut(s) 457, 913, 977, 2746, 3127
TaaI ACNGT 4 cut(s) 2720, 3293, 3385, 3815
TaiI ACGT 9 cut(s) 880, 1455, 1745, 2678, 3029, 3095, 3486, 3638, 3805
TaqI TCGA 3 cut(s) 28, 3288, 3433
TatI WGTACW 3 cut(s) 410, 2095, 3302
TauI GCSGC 2 cut(s) 1542, 3852
TscAI CASTG 4 cut(s) 774, 949, 2004, 2054
TseFI GTSAC 4 cut(s) 113, 931, 2354, 3507
TseI GCWGC 8 cut(s) 59, 109, 764, 2760, 3012, 3078, 3186, 3189
Tsp45I GTSAC 4 cut(s) 113, 931, 2354, 3507
TspGWI ACGGA 5 cut(s) 3263, 3270, 3404, 3507, 3751
TspRI CASTG 4 cut(s) 774, 949, 2004, 2054
Tth111I GACNNNGTC 1 cut(s) 1337
Van91I CCANNNNNTGG 2 cut(s) 571, 3128
Vha464I CTTAAG 1 cut(s) 2546
VpaK11BI GGWCC 2 cut(s) 1907, 3515
XagI CCTNNNNNAGG 1 cut(s) 2898
XapI RAATTY 7 cut(s) 8, 1262, 1301, 1609, 1833, 2130, 3621
XbaI TCTAGA 2 cut(s) 2872, 3376
XceI RCATGY 7 cut(s) 227, 1485, 1811, 1979, 2030, 2273, 3453
XcmI CCANNNNNNNNNTGG 4 cut(s) 235, 604, 1384, 3466
XhoI CTCGAG 1 cut(s) 27
XmaJI CCTAGG 1 cut(s) 977
XmiI GTMKAC 2 cut(s) 2062, 3454
XmnI GAANNNNTTC 3 cut(s) 1054, 2235, 3621
XspI CTAG 9 cut(s) 896, 978, 1911, 2187, 2658, 2873, 2900, 3182, 3377
ZraI GACGTC 1 cut(s) 3636
ZrmI AGTACT 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.