FvH4_7g01580

reductase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000539
Physical Location & Seq
Reverse (-)
1974499 .. 1987425
12927 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g01580.t1

Sequence Viewer

Length: 1035 bp
ATGATAAAAAAAGCAATAAAGCAGTGCATAAATTCAAATCTGAAATCTGAAGGGTTGAAGAGAATGAGCGGAGCAGAGAAGGTGGTGGTGAGCGTCACCGGAGCCTCCGGCTACATAGCTTCATGGCTGGTCAAACTGTTGCTGCAACGCGGCTACACTGTCAAGGCTTCAGTTCGTGACCCAAATGACAAGAAGAAAACAGAACACTTGCTTTCATTAGATGGAGCCAAGGAAAGGCTTCAATTGTTCAAAGCAGATCTGCTGGAAGAAGGATCTTTTGATTCCTTAGTTGATGGTAGTGTAGCTGTTTTTCATACAGCATCCCCATTTTATCACAATCCCAGTGATCCGCAGGTTGAACTAATTGACCCTGCTTTGAAGGGAACACTTAATGTCCTCAGATCATGTGTTAAGGTTCCGTCTATCAAGAGGGTTATTATAACTTCCTCTATGGCAGCAGTTGCATTCAATGGAAAGCCTGTCGCTCCTGATGTAGTAATTGATGAGTCCTGGTTTTCAGATCCAGCTTTCTGTGAAAAATCAAAGGCTTGGTATATGCTGTCAAAGACATTAGCTGAGGAAGCTGCTTGGAAGTTTGCAAAAGAGAAAGGAATTGATATTGTTACAATAAATCCAGGTTTGGTGATCGGCCCTCTCTTACAGCCAACTCTCAACACAAGTGTGGAGCCAATTCTGAAACTCATAAATGGGACTGAAAAATTTCCAAACACAACTTATAGATTTGTTGATGTTAGAGATGTTGCCAACGCACATATTCTAGCCTTTGAGAATCCTTCAGCTAGTGGACGATATTGTTTAGTTGGAAGTGTAACACACTGTTCAGATATTGTGAAAATTCTGCGTGATATCTTCCCTGCTCTCAGTCTTCCAGAAAAGTCTGCAGATGATAAACCTTTCACTCCAACGTATCAGGTGTCCAAGGAAAGAGCCGAAACTTTGGGAGTCAAGTATACTGCGCTGGAAGTGACACTAAAGGATACTGTTGAAAGTTTGAAGGACAAGAATTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
3Beta_HSD Epimerase

Protein Analysis

345

Amino Acids

37.81

Weight (kDa)

8.61

Isoelectric Point (pI)

27.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 28 - 126 1.7e-10 NmrA-like family
Epimerase PF01370 31 - 268 5.4e-24 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 32 - 264 7.3e-17 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 32 - 221 3.2e-11 Male sterility protein
GDP_Man_Dehyd PF16363 32 - 223 1.4e-09 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 34 - 154 3.7e-09 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000539)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G51410 AT5G19440
fragaria_vesca FvH4_3g28560 FvH4_3g28560 FvH4_3g28560 FvH4_7g01561 FvH4_7g01563 FvH4_7g01563 FvH4_7g01564 FvH4_7g01580 FvH4_7g01580 FvH4_7g01580 FvH4_7g05190
malus_domestica MD02G1302800.v1.1 MD02G1302900.v1.1 MD03G1172800.v1.1 MD07G1020700.v1.1 MD11G1190800.v1.1
prunus_persica Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.2G021600_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1 Prupe.4G253700_v2.0.a1
pyrus_communis pycom02g25510 pycom02g25520 pycom11g16630
rosa_chinensis RchiOBHm_Chr1g0316841 RchiOBHm_Chr1g0317331 RchiOBHm_Chr1g0317461 RchiOBHm_Chr1g0317621 RchiOBHm_Chr1g0317661 RchiOBHm_Chr1g0317681 RchiOBHm_Chr1g0317691 RchiOBHm_Chr1g0317701 RchiOBHm_Chr5g0052741
rosa_laevigata RLG00000030526 RLG00000030589 RLG00000030590 RLG00000030591 RLG00000030605 RLG00000034802
rosa_multiflora Rmu_co8392933.1_g000001 Rmu_sc0001158.1_g000003 Rmu_sc0003816.1_g000002 Rmu_sc0007650.1_g000001 Rmu_sc0007650.1_g000006 Rmu_sc0010265.1_g000014 Rmu_sc0019879.1_g000002 Rmu_ssc0000324.1_g000001
rosa_roxburghii Rroxscaffold_1G00028520 Rroxscaffold_4G00330220 Rroxscaffold_4G00330240 Rroxscaffold_4G00330250 Rroxscaffold_4G00330260 Rroxscaffold_4G00330270 Rroxscaffold_4G00330310 Rroxscaffold_4G00330480
rosa_rugosa Rorug01G0016000 Rorug01G0016500 Rorug01G0016700 Rorug01G0016800 Rorug01G0023900 Rorug05G0270400 Rorug05G0270500
rosa_samantha Rh1BG024200 Rh1BG024800 Rh1BG024900 Rh1BG025000 Rh1DG044100 Rh1DG044200 Rh1DG044400 Rh1DG044500 Rh1DG044800 Rh1DG045800 Rh5CG379500 Rh5DG369300
rosa_wichuraiana Rw1G001920 Rw1G002020 Rw1G002040 Rw1G002060 Rw1G002070 Rw1G002080 Rw5G032270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 440
Acc36I ACCTGC 1 cut(s) 343
AccBSI CCGCTC 1 cut(s) 69
AccI GTMKAC 1 cut(s) 971
AccII CGCG 1 cut(s) 150
AciI CCGC 3 cut(s) 69, 150, 350
AclWI GGATC 3 cut(s) 280, 341, 515
AcsI RAATTY 4 cut(s) 31, 719, 855, 1024
AcuI CTGAAG 3 cut(s) 69, 153, 780
AfiI CCNNNNNNNGG 1 cut(s) 234
AgsI TTSAA 9 cut(s) 36, 58, 242, 250, 359, 379, 469, 1007, 1015
AjnI CCWGG 2 cut(s) 509, 634
AleI CACNNNNGTG 1 cut(s) 680
AluBI AGCT 6 cut(s) 119, 305, 527, 575, 584, 800
AluI AGCT 6 cut(s) 119, 305, 527, 575, 584, 800
AlwI GGATC 3 cut(s) 280, 341, 515
AoxI GGCC 1 cut(s) 649
ApeKI GCWGC 3 cut(s) 142, 455, 584
ApoI RAATTY 4 cut(s) 31, 719, 855, 1024
Asp700I GAANNNNTTC 3 cut(s) 237, 720, 1028
AspLEI GCGC 1 cut(s) 979
AspS9I GGNCC 1 cut(s) 650
AsuHPI GGTGA 3 cut(s) 88, 100, 655
BbsI GAAGAC 1 cut(s) 878
BbvCI CCTCAGC 1 cut(s) 576
BbvI GCAGC 3 cut(s) 129, 467, 571
BccI CCATC 2 cut(s) 215, 287
BciT130I CCWGG 2 cut(s) 511, 636
BciVI GTATCC 1 cut(s) 991
BfaI CTAG 2 cut(s) 779, 801
BfmI CTRYAG 1 cut(s) 900
BfuAI ACCTGC 1 cut(s) 343
BfuI GTATCC 1 cut(s) 991
BglII AGATCT 1 cut(s) 256
BisI GCNGC 4 cut(s) 143, 151, 456, 585
BlsI GCNGC 4 cut(s) 144, 152, 457, 586
Bme1390I CCNGG 2 cut(s) 511, 636
BmgT120I GGNCC 1 cut(s) 650
BmiI GGNNCC 4 cut(s) 103, 226, 417, 687
BmrFI CCNGG 2 cut(s) 511, 636
BmrI ACTGGG 1 cut(s) 336
BmsI GCATC 1 cut(s) 329
BmuI ACTGGG 1 cut(s) 336
BpiI GAAGAC 1 cut(s) 878
Bpu10I CCTNAGC 1 cut(s) 576
BsaJI CCNNGG 2 cut(s) 228, 939
BsaWI WCCGGW 1 cut(s) 98
Bsc4I CCNNNNNNNGG 1 cut(s) 234
Bse1I ACTGG 1 cut(s) 342
BseBI CCWGG 2 cut(s) 511, 636
BseDI CCNNGG 2 cut(s) 228, 939
BseGI GGATG 1 cut(s) 320
BseLI CCNNNNNNNGG 1 cut(s) 234
BseMII CTCAG 3 cut(s) 412, 567, 895
BseNI ACTGG 1 cut(s) 342
BseXI GCAGC 3 cut(s) 129, 467, 571
Bsh1236I CGCG 1 cut(s) 150
BshFI GGCC 1 cut(s) 651
BsiSI CCGG 2 cut(s) 99, 108
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 1 cut(s) 234
BsmFI GGGAC 1 cut(s) 724
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 1 cut(s) 651
Bsp143I GATC 6 cut(s) 256, 272, 346, 401, 520, 645
BspACI CCGC 3 cut(s) 69, 150, 350
BspANI GGCC 1 cut(s) 651
BspCNI CTCAG 3 cut(s) 411, 568, 894
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 4 cut(s) 103, 226, 417, 687
BspMAI CTGCAG 1 cut(s) 904
BspMI ACCTGC 1 cut(s) 343
BspPI GGATC 3 cut(s) 280, 341, 515
BsrBI CCGCTC 1 cut(s) 69
BsrI ACTGG 1 cut(s) 342
BssECI CCNNGG 2 cut(s) 228, 939
BssMI GATC 6 cut(s) 256, 272, 346, 401, 520, 645
BssNAI GTATAC 1 cut(s) 972
BssT1I CCWWGG 2 cut(s) 228, 939
Bst1107I GTATAC 1 cut(s) 972
Bst2UI CCWGG 2 cut(s) 511, 636
Bst4CI ACNGT 4 cut(s) 138, 160, 839, 1003
Bst6I CTCTTC 1 cut(s) 53
BstAPI GCANNNNNTGC 1 cut(s) 461
BstDEI CTNAG 4 cut(s) 286, 398, 576, 881
BstF5I GGATG 1 cut(s) 320
BstFNI CGCG 1 cut(s) 150
BstHHI GCGC 1 cut(s) 979
BstKTI GATC 6 cut(s) 259, 275, 349, 404, 523, 648
BstMBI GATC 6 cut(s) 256, 272, 346, 401, 520, 645
BstMWI GCNNNNNNNGC 2 cut(s) 461, 581
BstNI CCWGG 2 cut(s) 511, 636
BstSCI CCNGG 2 cut(s) 509, 634
BstSFI CTRYAG 1 cut(s) 900
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 3 cut(s) 129, 467, 571
BstV2I GAAGAC 1 cut(s) 878
BstX2I RGATCY 3 cut(s) 256, 272, 520
BstYI RGATCY 3 cut(s) 256, 272, 520
BstZ17I GTATAC 1 cut(s) 972
BsuI GTATCC 1 cut(s) 991
BsuRI GGCC 1 cut(s) 651
BtsCI GGATG 1 cut(s) 320
BtsI GCAGTG 1 cut(s) 29
BtsIMutI CAGTG 4 cut(s) 29, 156, 349, 835
BveI ACCTGC 1 cut(s) 343
CfoI GCGC 1 cut(s) 979
Cfr13I GGNCC 1 cut(s) 650
CseI GACGC 1 cut(s) 82
CviAII CATG 2 cut(s) 123, 405
DdeI CTNAG 4 cut(s) 286, 398, 576, 881
DpnI GATC 6 cut(s) 258, 274, 348, 403, 522, 647
DpnII GATC 6 cut(s) 256, 272, 346, 401, 520, 645
Eam1104I CTCTTC 1 cut(s) 53
EarI CTCTTC 1 cut(s) 53
Eco130I CCWWGG 2 cut(s) 228, 939
Eco32I GATATC 1 cut(s) 868
Eco57I CTGAAG 3 cut(s) 69, 153, 780
EcoRII CCWGG 2 cut(s) 509, 634
EcoRV GATATC 1 cut(s) 868
EcoT14I CCWWGG 2 cut(s) 228, 939
ErhI CCWWGG 2 cut(s) 228, 939
FaeI CATG 2 cut(s) 126, 408
FalI AAGNNNNNCTT 1 cut(s) 1013
FaqI GGGAC 1 cut(s) 724
FatI CATG 2 cut(s) 122, 404
FblI GTMKAC 1 cut(s) 971
Fnu4HI GCNGC 4 cut(s) 143, 151, 456, 585
FokI GGATG 1 cut(s) 307
Fsp4HI GCNGC 4 cut(s) 143, 151, 456, 585
FspBI CTAG 2 cut(s) 779, 801
GlaI GCGC 1 cut(s) 978
GluI GCNGC 4 cut(s) 143, 151, 456, 585
HaeIII GGCC 1 cut(s) 651
HapII CCGG 2 cut(s) 99, 108
HgaI GACGC 1 cut(s) 82
HhaI GCGC 1 cut(s) 979
Hin1II CATG 2 cut(s) 126, 408
Hin6I GCGC 1 cut(s) 977
HinP1I GCGC 1 cut(s) 977
HinfI GANTC 4 cut(s) 281, 506, 790, 963
HpaII CCGG 2 cut(s) 99, 108
HphI GGTGA 3 cut(s) 88, 100, 655
Hpy166II GTNNAC 2 cut(s) 806, 972
Hpy188I TCNGA 6 cut(s) 42, 49, 401, 520, 696, 844
Hpy188III TCNNGA 4 cut(s) 176, 427, 488, 890
Hpy8I GTNNAC 2 cut(s) 806, 972
HpyAV CCTTC 6 cut(s) 44, 73, 263, 373, 804, 1009
HpyCH4III ACNGT 4 cut(s) 138, 160, 839, 1003
HpyCH4IV ACGT 1 cut(s) 926
HpyCH4V TGCA 5 cut(s) 27, 145, 464, 599, 902
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 581
HpyF3I CTNAG 4 cut(s) 286, 398, 576, 881
HpySE526I ACGT 1 cut(s) 926
Hsp92II CATG 2 cut(s) 126, 408
HspAI GCGC 1 cut(s) 977
Kzo9I GATC 6 cut(s) 256, 272, 346, 401, 520, 645
LmnI GCTCC 5 cut(s) 71, 101, 224, 490, 685
Lsp1109I GCAGC 3 cut(s) 129, 467, 571
LweI GCATC 1 cut(s) 329
MaeI CTAG 2 cut(s) 779, 801
MaeII ACGT 1 cut(s) 926
MaeIII GTNAC 5 cut(s) 94, 176, 622, 829, 985
MalI GATC 6 cut(s) 258, 274, 348, 403, 522, 647
MbiI CCGCTC 1 cut(s) 69
MboI GATC 6 cut(s) 256, 272, 346, 401, 520, 645
MboII GAAGA 6 cut(s) 70, 205, 278, 862, 878, 1021
MfeI CAATTG 1 cut(s) 242
MflI RGATCY 3 cut(s) 256, 272, 520
MluCI AATT 9 cut(s) 31, 242, 363, 498, 612, 690, 719, 855, 1024
MlyI GAGTC 2 cut(s) 515, 972
MmeI TCCRAC 2 cut(s) 802, 947
MnlI CCTC 6 cut(s) 115, 407, 423, 457, 571, 663
MroXI GAANNNNTTC 3 cut(s) 237, 720, 1028
MseI TTAA 2 cut(s) 390, 411
MslI CAYNNNNRTG 1 cut(s) 680
MspI CCGG 2 cut(s) 99, 108
MspR9I CCNGG 2 cut(s) 511, 636
MunI CAATTG 1 cut(s) 242
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 2 cut(s) 511, 636
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 2 cut(s) 461, 581
NdeII GATC 6 cut(s) 256, 272, 346, 401, 520, 645
NlaIII CATG 2 cut(s) 126, 408
NlaIV GGNNCC 4 cut(s) 103, 226, 417, 687
NmuCI GTSAC 3 cut(s) 94, 176, 985
OliI CACNNNNGTG 1 cut(s) 680
PctI GAATGC 1 cut(s) 464
PdmI GAANNNNTTC 3 cut(s) 237, 720, 1028
PfeI GAWTC 2 cut(s) 281, 790
PkrI GCNGC 4 cut(s) 144, 152, 457, 586
PleI GAGTC 2 cut(s) 514, 971
PpsI GAGTC 2 cut(s) 514, 971
PsiI TTATAA 1 cut(s) 440
Psp6I CCWGG 2 cut(s) 509, 634
PspGI CCWGG 2 cut(s) 509, 634
PspN4I GGNNCC 4 cut(s) 103, 226, 417, 687
PspPI GGNCC 1 cut(s) 650
PstI CTGCAG 1 cut(s) 904
PsuI RGATCY 3 cut(s) 256, 272, 520
RseI CAYNNNNRTG 1 cut(s) 680
SaqAI TTAA 2 cut(s) 390, 411
SatI GCNGC 4 cut(s) 143, 151, 456, 585
Sau3AI GATC 6 cut(s) 256, 272, 346, 401, 520, 645
Sau96I GGNCC 1 cut(s) 650
SchI GAGTC 2 cut(s) 515, 972
ScrFI CCNGG 2 cut(s) 511, 636
SfaNI GCATC 1 cut(s) 329
SfcI CTRYAG 1 cut(s) 900
SmiMI CAYNNNNRTG 1 cut(s) 680
Sse9I AATT 9 cut(s) 31, 242, 363, 498, 612, 690, 719, 855, 1024
SsiI CCGC 3 cut(s) 69, 150, 350
SspMI CTAG 2 cut(s) 779, 801
StyD4I CCNGG 2 cut(s) 509, 634
StyI CCWWGG 2 cut(s) 228, 939
TaaI ACNGT 4 cut(s) 138, 160, 839, 1003
TaiI ACGT 1 cut(s) 929
TasI AATT 9 cut(s) 31, 242, 363, 498, 612, 690, 719, 855, 1024
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 281, 790
Tru1I TTAA 2 cut(s) 390, 411
Tru9I TTAA 2 cut(s) 390, 411
TscAI CASTG 4 cut(s) 29, 163, 349, 842
TseFI GTSAC 3 cut(s) 94, 176, 985
TseI GCWGC 3 cut(s) 142, 455, 584
Tsp45I GTSAC 3 cut(s) 94, 176, 985
TspDTI ATGAA 3 cut(s) 111, 204, 302
TspGWI ACGGA 1 cut(s) 408
TspRI CASTG 4 cut(s) 29, 163, 349, 842
XapI RAATTY 4 cut(s) 31, 719, 855, 1024
XmiI GTMKAC 1 cut(s) 971
XmnI GAANNNNTTC 3 cut(s) 237, 720, 1028
XspI CTAG 2 cut(s) 779, 801
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.