Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000775

Physical Location & Seq

Genomic Coordinates Reverse (-)

723596 .. 726954

Gene Structure 3359 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1011400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2331 bp

ATGAGGATATGTGTGAGAACTGAATTAGCTATTTCCATGGAATTCAGAAATCTCCCCTATCTGATCATCCCCCTCATCTTCTTTGTTTCTTGTTCCTCTGCACAAAGTTGGATAAGAGCCGGTTACTGGTACGCCGGCAGCGAGTCTCCTATACCGGAGATAAATTCTGCCTTGTTCACTCATCTTATCTGTGGTTTTGCTGATGTGAATGCCTCCACCTACCAGCTCTCCATCCCTTCTGCTCATGTCCAATACTTTTCGAGCTTCACCGATATTGTCAAGCGAAAGAACCCGTCCACCATCACAGTCCTATCCATCTGGAATGGACAGGCTGAAACAGGGCGAGCGATTTTGGGCGAGAACGCTAACTTTTCTGTCTTGTCCTCAATGCTCGAAAACCCTTCTAATAGAAGGTCTTTCATTGAGTCCTCCATCAAAATGGCAAGGCGTTATGGATTTCGGGGTATAGACTTATTCTGGCTGTGGCCTAGCGAAGCCTCCGATATGAATAACATGGGTAAGCTGTTGGACGAGTGGCGAGCTGCTGTGACTTATGAGTCGAGAAATTCAAGTCAGTCACAGCTATTGCTGACAATGGCCGTTCGTTACTTGCCTGCTTTTGAATCATTGAGTTACCCTATTGATTCTATGAAGAGGAATTTGGACTGGGCACATGTTGTGGCGTATGACTATCATCTGCCTTCGAAGGAGAATGTTACCGGTGCTCATGCGGCTTTATACAATCCTTTGAGCCATGTTAACACTGACTACGGTATAAGGCAATGGTTAAACTACTCATTTCCGGCCGGCAAACTGGTTTTGGGTTTGCCTTACCACGGATATGCATGGACACTTGCAAAACCCGAAGAAAATAATGGAATTGGGGCACCTGCATCTGGCACTGCTGTGACAAAAGATGGATCCATGAGCTATAAGTACATCAAGGGGTACACACGAAGCTACCAAGCTCCCATAGTGTATAATGCTACTTATGTTGTAAATTACTGCATCATAGGATCGAGTTGGGTTGGTTTCGATGATGTGGAAGCTATCAGAGCTAAGATTGCATATGCCAAGGAGAAGAAACTACTCGGTTCCAATGTGTTTCTAGTCACCAATGACGATGACAATTGGGCGCTTTCTCGGGCAGCTCAAGAGGAAGGAAATGGTCATGAAAACAAGCGGCGATTATTGCTTATAATTTTGCTTCCAGTTACTTCGATCGTAATTCTTACAGCCTTTGTGGTGTGTTACTTGCAGAGAAAACTCTTGATGACAAGAGGGAGAATAATTCTAGGCAATTCAAGAGCATCAGATCCTTCAAATCCGCAAGTGTTCAGCTTTTCTGAGATCAAAGCGGCAACAAATAAATTTTCAAGCGAAAATAAGCTTGGAGAGGGCGGGTTTGGTCCCGTATACAAGGGTAAACTGCGGACAGGACAGGAAATAGCTGTGAAAAGACTTTCGAAAACGTCAACTCAAGGACTTGAGGAATTTGGAAATGAGGTTACACTTACCGCGAACCTACAACATGTAAATTTAGTTCGGGTTCTTGGATTCTGCAGTGAGAAGGAAGAAAAAATGCTGATCTACGAGTACATGCCAAACAGAAGCTTGGACTTCTACCTTTTCGATCCTACCAGAAAGCAACTCCTAGACTGGAGAAAGCGTGTGCACATCATTGAAGGGGTTACTCAGGGACTCCTATATCTTCAAGAATACTCAAATGTTACTATCATTCACCGAGATCTAAAAGCTAGCAACATTTTGCTTGACAATGAGATGAATGCCAAAATTTCAGATTTTGGAATGGCCAAACTTTTCGGAAAAGATGAACTTGAAGGAAACACAAGCCGGATTGTTGGAACATACGGCTATGTTCCTCCTGAATATGTAAAAAAGGGCATATACTCTATGAAGTACGATGTCTATAGCTTTGGCGTTCTACTTTTGCAACTGATTAGCGGAAGGAAGAATACATGTTACTATGGTCCGAACAAAAGCTTGCACCTCTTGGAATATGCATATGCATCATGGAAAGAGGATAAAGGGCTGGAATTTATTGATCATTCACTTGACGATTCTTCTACTTCTTTTAAACTGTTGAGGTGCTTGCAAGTAGCTCTACTATGTGTCCAGGAAAATCCCGACGATAGGCCTACGATGTTGGAAGTCTATTCCCTACTCAAAACTGACACTGAGGCCATTCCAATTCCTACAACGACAGCTTTCTCTGGAAAAACTGATAGAAATATGGAAAGCGTATCTACATCACAGAAAGGAAGCTGTTCAGTCAATGATGCTGAGATTTCCGAACTGAAACCCCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Glyco_hydro_18 Pkinase Pkinase_Tyr

Protein Analysis

777

Amino Acids

87.38

Weight (kDa)

8.27

Isoelectric Point (pI)

38.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_18	PF00704	40 - 374	7.4e-60	Glycosyl hydrolases family 18
Pkinase	PF00069	459 - 725	2.4e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	461 - 727	7e-46	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000775)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g01350 FvH4_1g01363 FvH4_1g01365 FvH4_1g01383 FvH4_1g01390 FvH4_1g01420
malus_domestica	MD02G1011200.v1.1 MD02G1011400.v1.1 MD15G1156200.v1.1 MD15G1156300.v1.1 MD15G1156600.v1.1 MD15G1156700.v1.1 MD15G1156900.v1.1 MD15G1157000.v1.1
prunus_persica	Prupe.7G258600_v2.0.a1 Prupe.7G258700_v2.0.a1 Prupe.7G258700_v2.0.a1 Prupe.7G258800_v2.0.a1 Prupe.7G258900_v2.0.a1
pyrus_communis	pycom02g01010 pycom02g01020 pycom14g14440 pycom15g14020 pycom15g14030 pycom15g14050 pycom15g14060 pycom15g14080
rosa_chinensis	RchiOBHm_Chr2g0086191 RchiOBHm_Chr2g0086211 RchiOBHm_Chr2g0086231 RchiOBHm_Chr2g0086261 RchiOBHm_Chr2g0086281 RchiOBHm_Chr2g0086301 RchiOBHm_Chr2g0086311 RchiOBHm_Chr2g0086321 RchiOBHm_Chr2g0160801 RchiOBHm_Chr6g0247981 RchiOBHm_Chr6g0247991
rosa_laevigata	RLG00000015749
rosa_multiflora	Rmu_co8497771.1_g000001 Rmu_sc0004510.1_g000014 Rmu_sc0004510.1_g000018 Rmu_sc0004948.1_g000005 Rmu_sc0004948.1_g000017 Rmu_sc0004948.1_g000018 Rmu_sc0004948.1_g000024
rosa_roxburghii	Rroxscaffold_2G00154700 Rroxscaffold_2G00154710
rosa_rugosa	Rorug01G0466400 Rorug01G0466500 Rorug05G0529900
rosa_samantha	Rh2AG016200 Rh2AG016300 Rh2AG016500 Rh2AG016700 Rh2BG016300 Rh2BG016400 Rh2BG016500 Rh2CG017200 Rh2CG017300 Rh2CG017400 Rh2CG017500 Rh2CG017600 Rh2DG018200 Rh2DG018300 Rh6AG044800 Rh6BG039800
rosa_wichuraiana	Rw0G015400 Rw2G001400 Rw2G001410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1199
AarI	CACCTGC	1 cut(s)	898
AasI	GACNNNNNNGTC	1 cut(s)	556
Acc36I	ACCTGC	1 cut(s)	898
AccB1I	GGYRCC	1 cut(s)	886
AccI	GTMKAC	1 cut(s)	1416
AccII	CGCG	1 cut(s)	1520
AciI	CCGC	8 cut(s)	731, 1183, 1328, 1358, 1401, 1432, 1518, 1965
AclWI	GGATC	5 cut(s)	915, 928, 1024, 1310, 1628
AcoI	YGGCCR	3 cut(s)	597, 804, 1812
AcsI	RAATTY	9 cut(s)	41, 163, 565, 658, 1370, 1493, 1537, 1794, 2057
AfaI	GTAC	5 cut(s)	131, 938, 950, 1598, 1922
AfiI	CCNNNNNNNGG	4 cut(s)	126, 836, 896, 2154
AflIII	ACRYGT	3 cut(s)	673, 1531, 1979
AgeI	ACCGGT	1 cut(s)	719
AgsI	TTSAA	8 cut(s)	570, 623, 1305, 1323, 1377, 1685, 1715, 1841
AjnI	CCWGG	1 cut(s)	2136
AjuI	GAANNNNNNNTTGG	4 cut(s)	644, 676, 1374, 1406
AleI	CACNNNNGTG	1 cut(s)	905
Alw21I	GWGCWC	2 cut(s)	727, 1677
Alw26I	GTCTC	1 cut(s)	150
Alw44I	GTGCAC	1 cut(s)	1673
AlwI	GGATC	5 cut(s)	915, 928, 1024, 1310, 1628
Ama87I	CYCGRG	1 cut(s)	1143
AoxI	GGCC	6 cut(s)	485, 597, 804, 1812, 2156, 2202
ApaLI	GTGCAC	1 cut(s)	1673
ApeKI	GCWGC	3 cut(s)	138, 542, 1148
ApoI	RAATTY	9 cut(s)	41, 163, 565, 658, 1370, 1493, 1537, 1794, 2057
AsiGI	ACCGGT	1 cut(s)	719
Asp700I	GAANNNNTTC	1 cut(s)	2287
AspLEI	GCGC	1 cut(s)	1138
AspS9I	GGNCC	2 cut(s)	1409, 1991
AsuHPI	GGTGA	3 cut(s)	259, 1105, 1733
AsuII	TTCGAA	2 cut(s)	704, 1466
AsuNHI	GCTAGC	1 cut(s)	1757
AvaI	CYCGRG	1 cut(s)	1143
AvaII	GGWCC	2 cut(s)	1409, 1991
BaeGI	GKGCMC	3 cut(s)	673, 889, 1677
BalI	TGGCCA	1 cut(s)	1814
BamHI	GGATCC	1 cut(s)	920
BanI	GGYRCC	1 cut(s)	886
BarI	GAAGNNNNNNTAC	2 cut(s)	1960, 1992
Bbv12I	GWGCWC	2 cut(s)	727, 1677
BbvI	GCAGC	3 cut(s)	150, 529, 1160
BccI	CCATC	5 cut(s)	239, 308, 323, 440, 911
BceAI	ACGGC	2 cut(s)	584, 1888
BciT130I	CCWGG	1 cut(s)	2138
BclI	TGATCA	2 cut(s)	63, 2065
BcoDI	GTCTC	1 cut(s)	150
BfaI	CTAG	5 cut(s)	489, 1109, 1295, 1655, 1758
BfmI	CTRYAG	2 cut(s)	1561, 1930
BfoI	RGCGCY	1 cut(s)	1139
BfuAI	ACCTGC	1 cut(s)	898
BglII	AGATCT	1 cut(s)	1747
BisI	GCNGC	6 cut(s)	139, 543, 732, 1149, 1184, 1359
BlsI	GCNGC	6 cut(s)	140, 544, 733, 1150, 1185, 1360
Bme1390I	CCNGG	1 cut(s)	2138
Bme18I	GGWCC	2 cut(s)	1409, 1991
BmeT110I	CYCGRG	1 cut(s)	1143
BmgT120I	GGNCC	2 cut(s)	1409, 1991
BmiI	GGNNCC	4 cut(s)	888, 922, 1096, 1411
BmrFI	CCNGG	1 cut(s)	2138
BmrI	ACTGGG	1 cut(s)	676
BmsI	GCATC	5 cut(s)	902, 1017, 1319, 2039, 2290
BmtI	GCTAGC	1 cut(s)	1761
BmuI	ACTGGG	1 cut(s)	676
BpmI	CTGGAG	1 cut(s)	1681
Bpu14I	TTCGAA	2 cut(s)	704, 1466
BpuEI	CTTGAG	3 cut(s)	1137, 1464, 1508
BsaJI	CCNNGG	3 cut(s)	36, 835, 1074
BsaWI	WCCGGW	2 cut(s)	154, 719
BsaXI	ACNNNNNCTCC	4 cut(s)	212, 242, 1654, 1684
Bsc4I	CCNNNNNNNGG	4 cut(s)	126, 836, 896, 2154
Bse118I	RCCGGY	4 cut(s)	119, 134, 719, 806
Bse1I	ACTGG	5 cut(s)	131, 671, 819, 1211, 1664
Bse3DI	GCAATG	1 cut(s)	788
BseBI	CCWGG	1 cut(s)	2138
BseDI	CCNNGG	3 cut(s)	36, 835, 1074
BseGI	GGATG	2 cut(s)	66, 231
BseLI	CCNNNNNNNGG	4 cut(s)	126, 836, 896, 2154
BseMI	GCAATG	1 cut(s)	788
BseMII	CTCAG	4 cut(s)	1338, 1709, 2190, 2295
BseNI	ACTGG	5 cut(s)	131, 671, 819, 1211, 1664
BseSI	GKGCMC	3 cut(s)	673, 889, 1677
BseX3I	CGGCCG	1 cut(s)	804
BseXI	GCAGC	3 cut(s)	150, 529, 1160
BsgI	GTGCAG	1 cut(s)	84
Bsh1236I	CGCG	1 cut(s)	1520
Bsh1285I	CGRYCG	2 cut(s)	807, 1224
BshFI	GGCC	6 cut(s)	487, 599, 806, 1814, 2158, 2204
BshNI	GGYRCC	1 cut(s)	886
BshTI	ACCGGT	1 cut(s)	719
BsiEI	CGRYCG	2 cut(s)	807, 1224
BsiHKAI	GWGCWC	2 cut(s)	727, 1677
BsiHKCI	CYCGRG	1 cut(s)	1143
BsiSI	CCGG	7 cut(s)	120, 135, 155, 720, 803, 807, 1855
BslFI	GGGAC	2 cut(s)	1395, 1713
BslI	CCNNNNNNNGG	4 cut(s)	126, 836, 896, 2154
BsmAI	GTCTC	1 cut(s)	150
BsmFI	GGGAC	2 cut(s)	1395, 1713
BsmI	GAATGC	2 cut(s)	214, 1792
BsnI	GGCC	6 cut(s)	487, 599, 806, 1814, 2158, 2204
BsoBI	CYCGRG	1 cut(s)	1143
Bsp119I	TTCGAA	2 cut(s)	704, 1466
Bsp1286I	GDGCHC	4 cut(s)	673, 727, 889, 1677
Bsp19I	CCATGG	1 cut(s)	36
BspACI	CCGC	8 cut(s)	731, 1183, 1328, 1358, 1401, 1432, 1518, 1965
BspANI	GGCC	6 cut(s)	487, 599, 806, 1814, 2158, 2204
BspCNI	CTCAG	4 cut(s)	1339, 1708, 2191, 2296
BspFNI	CGCG	1 cut(s)	1520
BspHI	TCATGA	1 cut(s)	1171
BspLI	GGNNCC	4 cut(s)	888, 922, 1096, 1411
BspMAI	CTGCAG	1 cut(s)	1565
BspMI	ACCTGC	1 cut(s)	898
BspOI	GCTAGC	1 cut(s)	1761
BspPI	GGATC	5 cut(s)	915, 928, 1024, 1310, 1628
BspT104I	TTCGAA	2 cut(s)	704, 1466
BspT107I	GGYRCC	1 cut(s)	886
BsrDI	GCAATG	1 cut(s)	788
BsrFI	RCCGGY	4 cut(s)	119, 134, 719, 806
BsrI	ACTGG	5 cut(s)	131, 671, 819, 1211, 1664
BssAI	RCCGGY	4 cut(s)	119, 134, 719, 806
BssECI	CCNNGG	3 cut(s)	36, 835, 1074
BssNAI	GTATAC	1 cut(s)	1417
BssT1I	CCWWGG	2 cut(s)	36, 1074
Bst1107I	GTATAC	1 cut(s)	1417
Bst2UI	CCWGG	1 cut(s)	2138
Bst4CI	ACNGT	3 cut(s)	307, 773, 2103
Bst6I	CTCTTC	1 cut(s)	647
BstBI	TTCGAA	2 cut(s)	704, 1466
BstC8I	GCNNGC	8 cut(s)	136, 345, 540, 615, 808, 1759, 2006, 2114
BstDEI	CTNAG	5 cut(s)	1059, 1347, 1695, 2199, 2304
BstDSI	CCRYGG	2 cut(s)	36, 835
BstF5I	GGATG	2 cut(s)	66, 231
BstFNI	CGCG	1 cut(s)	1520
BstH2I	RGCGCY	1 cut(s)	1139
BstHHI	GCGC	1 cut(s)	1138
BstMAI	GTCTC	1 cut(s)	150
BstMCI	CGRYCG	2 cut(s)	807, 1224
BstMWI	GCNNNNNNNGC	4 cut(s)	731, 1055, 1064, 1192
BstNI	CCWGG	1 cut(s)	2138
BstNSI	RCATGY	4 cut(s)	677, 1535, 1603, 1983
BstSCI	CCNGG	1 cut(s)	2136
BstSFI	CTRYAG	2 cut(s)	1561, 1930
BstSLI	GKGCMC	3 cut(s)	673, 889, 1677
BstUI	CGCG	1 cut(s)	1520
BstV1I	GCAGC	3 cut(s)	150, 529, 1160
BstX2I	RGATCY	3 cut(s)	920, 1315, 1747
BstXI	CCANNNNNNTGG	1 cut(s)	439
BstYI	RGATCY	3 cut(s)	920, 1315, 1747
BstZ17I	GTATAC	1 cut(s)	1417
BstZI	CGGCCG	1 cut(s)	804
BsuRI	GGCC	6 cut(s)	487, 599, 806, 1814, 2158, 2204
BtgI	CCRYGG	2 cut(s)	36, 835
BtsCI	GGATG	2 cut(s)	66, 231
BtsI	GCAGTG	2 cut(s)	900, 1570
BtsIMutI	CAGTG	4 cut(s)	762, 900, 1570, 2196
BveI	ACCTGC	1 cut(s)	898
Cac8I	GCNNGC	8 cut(s)	136, 345, 540, 615, 808, 1759, 2006, 2114
CciI	TCATGA	1 cut(s)	1171
CfoI	GCGC	1 cut(s)	1138
Cfr10I	RCCGGY	4 cut(s)	119, 134, 719, 806
Cfr13I	GGNCC	2 cut(s)	1409, 1991
Csp6I	GTAC	5 cut(s)	130, 937, 949, 1597, 1921
CspAI	ACCGGT	1 cut(s)	719
CviQI	GTAC	5 cut(s)	130, 937, 949, 1597, 1921
DdeI	CTNAG	5 cut(s)	1059, 1347, 1695, 2199, 2304
DraI	TTTAAA	1 cut(s)	2098
DrdI	GACNNNNNNGTC	1 cut(s)	556
DseDI	GACNNNNNNGTC	1 cut(s)	556
EaeI	YGGCCR	3 cut(s)	597, 804, 1812
EagI	CGGCCG	1 cut(s)	804
Eam1104I	CTCTTC	1 cut(s)	647
EarI	CTCTTC	1 cut(s)	647
EclXI	CGGCCG	1 cut(s)	804
Eco130I	CCWWGG	2 cut(s)	36, 1074
Eco147I	AGGCCT	1 cut(s)	2158
Eco47I	GGWCC	2 cut(s)	1409, 1991
Eco52I	CGGCCG	1 cut(s)	804
Eco88I	CYCGRG	1 cut(s)	1143
EcoRI	GAATTC	1 cut(s)	41
EcoRII	CCWGG	1 cut(s)	2136
EcoT14I	CCWWGG	2 cut(s)	36, 1074
EcoT22I	ATGCAT	3 cut(s)	847, 2026, 2032
ErhI	CCWWGG	2 cut(s)	36, 1074
FalI	AAGNNNNNCTT	2 cut(s)	1821, 1853
FaqI	GGGAC	2 cut(s)	1395, 1713
FauI	CCCGC	1 cut(s)	1394
FauNDI	CATATG	2 cut(s)	1069, 2026
FbaI	TGATCA	2 cut(s)	63, 2065
FblI	GTMKAC	1 cut(s)	1416
Fnu4HI	GCNGC	6 cut(s)	139, 543, 732, 1149, 1184, 1359
FokI	GGATG	2 cut(s)	53, 218
Fsp4HI	GCNGC	6 cut(s)	139, 543, 732, 1149, 1184, 1359
FspBI	CTAG	5 cut(s)	489, 1109, 1295, 1655, 1758
GlaI	GCGC	1 cut(s)	1137
GluI	GCNGC	6 cut(s)	139, 543, 732, 1149, 1184, 1359
GsuI	CTGGAG	1 cut(s)	1681
HaeII	RGCGCY	1 cut(s)	1139
HaeIII	GGCC	6 cut(s)	487, 599, 806, 1814, 2158, 2204
HapII	CCGG	7 cut(s)	120, 135, 155, 720, 803, 807, 1855
HhaI	GCGC	1 cut(s)	1138
Hin6I	GCGC	1 cut(s)	1136
HinP1I	GCGC	1 cut(s)	1136
HincII	GTYRAC	2 cut(s)	760, 1476
HindII	GTYRAC	2 cut(s)	760, 1476
HindIII	AAGCTT	3 cut(s)	1388, 1612, 2002
HinfI	GANTC	8 cut(s)	143, 425, 557, 623, 644, 1557, 1701, 2081
HpaI	GTTAAC	1 cut(s)	760
HpaII	CCGG	7 cut(s)	120, 135, 155, 720, 803, 807, 1855
HphI	GGTGA	3 cut(s)	259, 1105, 1733
Hpy166II	GTNNAC	8 cut(s)	177, 297, 760, 951, 1417, 1427, 1476, 1675
Hpy8I	GTNNAC	8 cut(s)	177, 297, 760, 951, 1417, 1427, 1476, 1675
Hpy99I	CGWCG	1 cut(s)	2153
HpyCH4III	ACNGT	3 cut(s)	307, 773, 2103
HpyCH4IV	ACGT	1 cut(s)	1472
HpyF10VI	GCNNNNNNNGC	4 cut(s)	731, 1055, 1064, 1192
HpyF3I	CTNAG	5 cut(s)	1059, 1347, 1695, 2199, 2304
HpySE526I	ACGT	1 cut(s)	1472
HspAI	GCGC	1 cut(s)	1136
KroI	GCCGGC	2 cut(s)	134, 806
KroNI	GCCGGC	2 cut(s)	136, 808
Ksp22I	TGATCA	2 cut(s)	63, 2065
KspAI	GTTAAC	1 cut(s)	760
LmnI	GCTCC	1 cut(s)	973
Lsp1109I	GCAGC	3 cut(s)	150, 529, 1160
LweI	GCATC	5 cut(s)	902, 1017, 1319, 2039, 2290
MaeI	CTAG	5 cut(s)	489, 1109, 1295, 1655, 1758
MaeII	ACGT	1 cut(s)	1472
MboII	GAAGA	8 cut(s)	70, 664, 878, 1093, 1586, 1703, 1984, 2076
MfeI	CAATTG	1 cut(s)	1129
MflI	RGATCY	3 cut(s)	920, 1315, 1747
MhlI	GDGCHC	4 cut(s)	673, 727, 889, 1677
MlsI	TGGCCA	1 cut(s)	1814
MluNI	TGGCCA	1 cut(s)	1814
MlyI	GAGTC	4 cut(s)	152, 434, 566, 1695
MmeI	TCCRAC	4 cut(s)	89, 507, 1843, 2148
Mox20I	TGGCCA	1 cut(s)	1814
Mph1103I	ATGCAT	3 cut(s)	847, 2026, 2032
MroNI	GCCGGC	2 cut(s)	134, 806
MroXI	GAANNNNTTC	1 cut(s)	2287
MscI	TGGCCA	1 cut(s)	1814
MseI	TTAA	3 cut(s)	759, 788, 2097
MslI	CAYNNNNRTG	3 cut(s)	437, 840, 905
Msp20I	TGGCCA	1 cut(s)	1814
MspI	CCGG	7 cut(s)	120, 135, 155, 720, 803, 807, 1855
MspR9I	CCNGG	1 cut(s)	2138
MunI	CAATTG	1 cut(s)	1129
Mva1269I	GAATGC	2 cut(s)	214, 1792
MvaI	CCWGG	1 cut(s)	2138
MvnI	CGCG	1 cut(s)	1520
MwoI	GCNNNNNNNGC	4 cut(s)	731, 1055, 1064, 1192
NaeI	GCCGGC	2 cut(s)	136, 808
NcoI	CCATGG	1 cut(s)	36
NdeI	CATATG	2 cut(s)	1069, 2026
NgoMIV	GCCGGC	2 cut(s)	134, 806
NheI	GCTAGC	1 cut(s)	1757
NlaIV	GGNNCC	4 cut(s)	888, 922, 1096, 1411
NmuCI	GTSAC	4 cut(s)	547, 576, 907, 1111
NsiI	ATGCAT	3 cut(s)	847, 2026, 2032
NspI	RCATGY	4 cut(s)	677, 1535, 1603, 1983
NspV	TTCGAA	2 cut(s)	704, 1466
OliI	CACNNNNGTG	1 cut(s)	905
PagI	TCATGA	1 cut(s)	1171
PaqCI	CACCTGC	1 cut(s)	898
PceI	AGGCCT	1 cut(s)	2158
PciI	ACATGT	3 cut(s)	673, 1531, 1979
PcsI	WCGNNNNNNNCGW	1 cut(s)	138
PctI	GAATGC	2 cut(s)	214, 1792
PdiI	GCCGGC	2 cut(s)	136, 808
PdmI	GAANNNNTTC	1 cut(s)	2287
PfeI	GAWTC	4 cut(s)	623, 644, 1557, 2081
PfoI	TCCNGGA	1 cut(s)	2136
PinAI	ACCGGT	1 cut(s)	719
PkrI	GCNGC	6 cut(s)	140, 544, 733, 1150, 1185, 1360
Ple19I	CGATCG	1 cut(s)	1224
PleI	GAGTC	4 cut(s)	151, 433, 565, 1695
PpsI	GAGTC	4 cut(s)	151, 433, 565, 1695
PscI	ACATGT	3 cut(s)	673, 1531, 1979
PsiI	TTATAA	1 cut(s)	1199
Psp6I	CCWGG	1 cut(s)	2136
PspGI	CCWGG	1 cut(s)	2136
PspN4I	GGNNCC	4 cut(s)	888, 922, 1096, 1411
PspPI	GGNCC	2 cut(s)	1409, 1991
PsrI	GAACNNNNNNTAC	2 cut(s)	1527, 1559
PstI	CTGCAG	1 cut(s)	1565
PsuI	RGATCY	3 cut(s)	920, 1315, 1747
PvuI	CGATCG	1 cut(s)	1224
RsaI	GTAC	5 cut(s)	131, 938, 950, 1598, 1922
RsaNI	GTAC	5 cut(s)	130, 937, 949, 1597, 1921
RseI	CAYNNNNRTG	3 cut(s)	437, 840, 905
SaqAI	TTAA	3 cut(s)	759, 788, 2097
SatI	GCNGC	6 cut(s)	139, 543, 732, 1149, 1184, 1359
Sau96I	GGNCC	2 cut(s)	1409, 1991
SchI	GAGTC	4 cut(s)	152, 434, 566, 1695
ScrFI	CCNGG	1 cut(s)	2138
SduI	GDGCHC	4 cut(s)	673, 727, 889, 1677
SfaNI	GCATC	5 cut(s)	902, 1017, 1319, 2039, 2290
SfcI	CTRYAG	2 cut(s)	1561, 1930
SfuI	TTCGAA	2 cut(s)	704, 1466
SinI	GGWCC	2 cut(s)	1409, 1991
SmiMI	CAYNNNNRTG	3 cut(s)	437, 840, 905
SmlI	CTYRAG	3 cut(s)	1152, 1479, 1487
SmoI	CTYRAG	3 cut(s)	1152, 1479, 1487
SseBI	AGGCCT	1 cut(s)	2158
SsiI	CCGC	8 cut(s)	731, 1183, 1328, 1358, 1401, 1432, 1518, 1965
SspMI	CTAG	5 cut(s)	489, 1109, 1295, 1655, 1758
StuI	AGGCCT	1 cut(s)	2158
StyD4I	CCNGG	1 cut(s)	2136
StyI	CCWWGG	2 cut(s)	36, 1074
TaaI	ACNGT	3 cut(s)	307, 773, 2103
TaiI	ACGT	1 cut(s)	1475
TaqI	TCGA	9 cut(s)	260, 393, 560, 704, 1019, 1035, 1220, 1466, 1632
TatI	WGTACW	2 cut(s)	936, 1596
TauI	GCSGC	3 cut(s)	734, 1186, 1361
TfiI	GAWTC	4 cut(s)	623, 644, 1557, 2081
Tru1I	TTAA	3 cut(s)	759, 788, 2097
Tru9I	TTAA	3 cut(s)	759, 788, 2097
TscAI	CASTG	4 cut(s)	769, 907, 1570, 2203
TseFI	GTSAC	4 cut(s)	547, 576, 907, 1111
TseI	GCWGC	3 cut(s)	138, 542, 1148
Tsp45I	GTSAC	4 cut(s)	547, 576, 907, 1111
TspDTI	ATGAA	7 cut(s)	409, 521, 665, 1188, 1799, 1848, 1931
TspGWI	ACGGA	1 cut(s)	852
TspRI	CASTG	4 cut(s)	769, 907, 1570, 2203
VneI	GTGCAC	1 cut(s)	1673
VpaK11BI	GGWCC	2 cut(s)	1409, 1991
XapI	RAATTY	9 cut(s)	41, 163, 565, 658, 1370, 1493, 1537, 1794, 2057
XceI	RCATGY	4 cut(s)	677, 1535, 1603, 1983
XmiI	GTMKAC	1 cut(s)	1416
XmnI	GAANNNNTTC	1 cut(s)	2287
XspI	CTAG	5 cut(s)	489, 1109, 1295, 1655, 1758
Zsp2I	ATGCAT	3 cut(s)	847, 2026, 2032

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.