Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000775

Physical Location & Seq

Genomic Coordinates Forward (+)

11612837 .. 11615954

Gene Structure 3118 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1156300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2079 bp

ATGGTATCGAAAAATATCTCCCACCCGATCCTCTTCATCTTCCTTGTTGCTTGTAATTTTTCATGTTCTTCTGCACAAACTTGGATTCGAGCTGGTTACTGGTACACCGGCAGCGACTTTCCTTTACCGGAGGTAAACTCTGCCTTGTTCACACATCTTATTTGTGCTTTTGCCAATGTGAATTTCTCCACCTACCAGCTCTCCACCCCTTCTGCTCATGAACAAAACTTCTCGAGCTTCACCGATATTGTCAAGCGCAAAAACCCATCAATCACAACGCTCCTATCCATATGGAACGGAAAAGCAGCAACAACTCAGCTAATTTTGGGTCAGAGAGCTAACTTTTCAGTCTTGTCTAAAATGCTCGAAAACCCTTCGAATAGAAAGTCTTTCATTGAGTCTTCCATCAATGTGGCAAGACGTTATGGCTTCCAAGGTATAGACTTACTCTGGCCTTGGCTTAACACAGCCTCATATATGATCAATATGGAAAAGCTACTGGACGAGTGGCGAGCTGCTGTGACTTCTGAGGCAAGAAACTCTGATCTGCCACGATTAACATTGACAATGGCGGTTAGGTATATTCCCACTTTTGAATCGTTGATTTATCCAGTTGAATCTATGAAGAGGAATTTGGATTGGGCGCATGTTGTGGCGTATGACTACCATCTTCATTTAAAGGAGAATGTTACTGGTGCTTATGCTGCTTTATATGATCATTCGAGCTATGTTAACACCGACTATGGGATAAAGCAATGGTTAAACTACTCATTTCCGGCCAGCAAACTGGTTTTGGGTTTGCCTTACCACAGCTATGCGTGGACAATTGCAAACCCCAAAGACAATAATGGAATTGGGGCACCTGCATCTGGCTTTGCTGTGACAAAAGATGGATCAATGAGCTATAAGCTCATCAAGTGGTGCACTCGAAGCTACGGAGGGCTCATAGCGTATAATGATACTTATGTCGTGAATTACTGCACGGTAGGATCGACTTGGATTAATTTCGATGATGTGGAAGCTATCAGATCTAAGATTGCATATGTGAAAGCGAAGAAGCTACTCGGTACCAATGTGTTTCAAGTCGGCAATGATGATGAAATTTGGGCTCTTTCTCGGGCAGGTAGGCAATACTTATCCCATAAGCAATGTTTATTATCGATGAAGATAAGGCAAAGAGATTTCCTAAGATGTGTTCCAACATGTCATACAACTCGAGGTAAGTTGCGGAGAGGTCCGGAAATAGCAGTGAAAAGGCTTTCGAAAACGTCTACTCAAGGACTTCAGGAATTCGAACATGAGGTTAAACTTACTGCGAGACTACAACATATGAATCTAGTTCCTGTTCTTGGATTTTGCACTGAGAAGGAAGAAAAAATGCTGATCTACAAATGGTATTTCATGGATTGGGGAAAGCATGTGCACATCATCGAAGGCGTTACTCAAGGACTCCTATATCTTCAAGAATACTCAAACTTCACAATCATTCACCGTGATTTAAAAGCTAGCAACATTTTGCTTGATGACGAGATGAATGCTATGATTTCCGATTTTGGAATGGCCAAACTTTTCAGAAAGGATGAACTCGAAGGAAACACAAGGCGGATTGTTGGCACATATGGCTACGTACCTCCTGAATACGTACGAAATGGCATATACTCTATGAAGTACGACGTCTACAGCTTCGGAGTTCTACTTTTGCAAATGATTAGCGGACGGAAGAGTACACATTACTATGGTCCGAACGAAAGTATGCACCTCTTGGAATATGCATATGTATCATGGAAAGAGGACAAAGGACTGGAATTTATCGATCCGTCACTTGACGATTCTTCTTCTTCTTGTAAGTTATTGAGATGCTTGCAAGTGGCACTACTCTGTGTCCAGGAAAATGCCGACGATAGGCCTACAATGCTGGAAGTTTATTCCATGCTCAAATCTGACACTCAAGCCGTTTCAACTCCTACTAAGACGGCTTTCTCAGTAAAAAATTACGACAATGTGGACAACATATGTACCCCGCAGCCAGGAAATTGTTCGGTTAATGATGCTCAGATCTCCGAACTGCAACCCCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Glyco_hydro_18 Pkinase Pkinase_Tyr

Protein Analysis

693

Amino Acids

78.85

Weight (kDa)

8.44

Isoelectric Point (pI)

38.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_18	PF00704	40 - 365	2.1e-53	Glycosyl hydrolases family 18
PK_Tyr_Ser-Thr	PF07714	411 - 644	2e-27	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	411 - 641	4.1e-28	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000775)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g01350 FvH4_1g01363 FvH4_1g01365 FvH4_1g01383 FvH4_1g01390 FvH4_1g01420
malus_domestica	MD02G1011200.v1.1 MD02G1011400.v1.1 MD15G1156200.v1.1 MD15G1156300.v1.1 MD15G1156600.v1.1 MD15G1156700.v1.1 MD15G1156900.v1.1 MD15G1157000.v1.1
prunus_persica	Prupe.7G258600_v2.0.a1 Prupe.7G258700_v2.0.a1 Prupe.7G258700_v2.0.a1 Prupe.7G258800_v2.0.a1 Prupe.7G258900_v2.0.a1
pyrus_communis	pycom02g01010 pycom02g01020 pycom14g14440 pycom15g14020 pycom15g14030 pycom15g14050 pycom15g14060 pycom15g14080
rosa_chinensis	RchiOBHm_Chr2g0086191 RchiOBHm_Chr2g0086211 RchiOBHm_Chr2g0086231 RchiOBHm_Chr2g0086261 RchiOBHm_Chr2g0086281 RchiOBHm_Chr2g0086301 RchiOBHm_Chr2g0086311 RchiOBHm_Chr2g0086321 RchiOBHm_Chr2g0160801 RchiOBHm_Chr6g0247981 RchiOBHm_Chr6g0247991
rosa_laevigata	RLG00000015749
rosa_multiflora	Rmu_co8497771.1_g000001 Rmu_sc0004510.1_g000014 Rmu_sc0004510.1_g000018 Rmu_sc0004948.1_g000005 Rmu_sc0004948.1_g000017 Rmu_sc0004948.1_g000018 Rmu_sc0004948.1_g000024
rosa_roxburghii	Rroxscaffold_2G00154700 Rroxscaffold_2G00154710
rosa_rugosa	Rorug01G0466400 Rorug01G0466500 Rorug05G0529900
rosa_samantha	Rh2AG016200 Rh2AG016300 Rh2AG016500 Rh2AG016700 Rh2BG016300 Rh2BG016400 Rh2BG016500 Rh2CG017200 Rh2CG017300 Rh2CG017400 Rh2CG017500 Rh2CG017600 Rh2DG018200 Rh2DG018300 Rh6AG044800 Rh6BG039800
rosa_wichuraiana	Rw0G015400 Rw2G001400 Rw2G001410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	871
AatII	GACGTC	1 cut(s)	1677
Acc36I	ACCTGC	2 cut(s)	871, 1112
Acc65I	GGTACC	1 cut(s)	1067
AccB1I	GGYRCC	2 cut(s)	859, 1067
AccI	GTMKAC	2 cut(s)	1271, 1677
AccIII	TCCGGA	1 cut(s)	1237
AciI	CCGC	5 cut(s)	572, 1228, 1603, 1713, 2021
AclWI	GGATC	4 cut(s)	22, 901, 997, 1808
AcoI	YGGCCR	2 cut(s)	777, 1560
AcsI	RAATTY	5 cut(s)	181, 631, 1101, 1289, 1805
AcuI	CTGAAG	1 cut(s)	1268
AcyI	GRCGYC	1 cut(s)	1674
AfaI	GTAC	7 cut(s)	104, 1069, 1629, 1644, 1670, 1726, 2017
AfiI	CCNNNNNNNGG	5 cut(s)	744, 869, 1349, 1902, 2027
AflIII	ACRYGT	1 cut(s)	1202
AgsI	TTSAA	5 cut(s)	596, 617, 1082, 1463, 1959
AjnI	CCWGG	2 cut(s)	1884, 2026
AjuI	GAANNNNNNNTTGG	4 cut(s)	167, 199, 617, 649
Alw21I	GWGCWC	2 cut(s)	926, 1425
Alw26I	GTCTC	1 cut(s)	1312
Alw44I	GTGCAC	2 cut(s)	922, 1421
AlwI	GGATC	4 cut(s)	22, 901, 997, 1808
Ama87I	CYCGRG	3 cut(s)	232, 1116, 1215
Aor13HI	TCCGGA	1 cut(s)	1237
AoxI	GGCC	4 cut(s)	452, 777, 1560, 1904
ApaLI	GTGCAC	2 cut(s)	922, 1421
ApeKI	GCWGC	5 cut(s)	111, 305, 515, 704, 2023
ApoI	RAATTY	5 cut(s)	181, 631, 1101, 1289, 1805
ArsI	GACNNNNNNTTYG	2 cut(s)	371, 403
AseI	ATTAAT	1 cut(s)	1002
Asp700I	GAANNNNTTC	1 cut(s)	2035
Asp718I	GGTACC	1 cut(s)	1067
AspLEI	GCGC	2 cut(s)	258, 646
AspS9I	GGNCC	2 cut(s)	1235, 1739
AsuHPI	GGTGA	2 cut(s)	232, 1481
AsuII	TTCGAA	3 cut(s)	377, 1262, 1293
AsuNHI	GCTAGC	1 cut(s)	1505
AvaI	CYCGRG	3 cut(s)	232, 1116, 1215
AvaII	GGWCC	2 cut(s)	1235, 1739
BaeGI	GKGCMC	3 cut(s)	862, 926, 1425
BaeI	ACNNNNGTAYC	6 cut(s)	951, 984, 1059, 1092, 1999, 2032
BalI	TGGCCA	1 cut(s)	1562
BanI	GGYRCC	2 cut(s)	859, 1067
BanII	GRGCYC	2 cut(s)	945, 1111
BbsI	GAAGAC	1 cut(s)	393
Bbv12I	GWGCWC	2 cut(s)	926, 1425
BbvI	GCAGC	5 cut(s)	123, 317, 502, 691, 2035
BccI	CCATC	4 cut(s)	274, 413, 675, 884
BceAI	ACGGC	2 cut(s)	1937, 1989
BcgI	CGANNNNNNTGC	2 cut(s)	1517, 1551
BciT130I	CCWGG	2 cut(s)	1886, 2028
BclI	TGATCA	2 cut(s)	480, 715
BcoDI	GTCTC	1 cut(s)	1312
BfaI	CTAG	2 cut(s)	1337, 1506
BfmI	CTRYAG	1 cut(s)	1678
BfuAI	ACCTGC	2 cut(s)	871, 1112
BglII	AGATCT	2 cut(s)	1028, 2055
BisI	GCNGC	5 cut(s)	112, 306, 516, 705, 2024
BlsI	GCNGC	5 cut(s)	113, 307, 517, 706, 2025
Bme1390I	CCNGG	2 cut(s)	1886, 2028
Bme18I	GGWCC	2 cut(s)	1235, 1739
BmeT110I	CYCGRG	3 cut(s)	232, 1116, 1215
BmgT120I	GGNCC	2 cut(s)	1235, 1739
BmiI	GGNNCC	2 cut(s)	861, 1069
BmrFI	CCNGG	2 cut(s)	1886, 2028
BmsI	GCATC	3 cut(s)	875, 1847, 2038
BmtI	GCTAGC	1 cut(s)	1509
BpiI	GAAGAC	1 cut(s)	393
BplI	GAGNNNNNCTC	2 cut(s)	122, 154
Bpu14I	TTCGAA	3 cut(s)	377, 1262, 1293
BpuEI	CTTGAG	3 cut(s)	1260, 1428, 1932
Bsa29I	ATCGAT	2 cut(s)	1160, 1812
BsaAI	YACGTR	2 cut(s)	1627, 1642
BsaHI	GRCGYC	1 cut(s)	1674
BsaJI	CCNNGG	2 cut(s)	433, 455
BsaWI	WCCGGW	2 cut(s)	127, 1237
BsaXI	ACNNNNNCTCC	2 cut(s)	185, 215
Bsc4I	CCNNNNNNNGG	5 cut(s)	744, 869, 1349, 1902, 2027
Bse118I	RCCGGY	1 cut(s)	107
Bse1I	ACTGG	6 cut(s)	104, 504, 611, 697, 792, 1806
Bse3DI	GCAATG	3 cut(s)	761, 1096, 1154
BseAI	TCCGGA	1 cut(s)	1237
BseBI	CCWGG	2 cut(s)	1886, 2028
BseCI	ATCGAT	2 cut(s)	1160, 1812
BseDI	CCNNGG	2 cut(s)	433, 455
BseGI	GGATG	1 cut(s)	1585
BseLI	CCNNNNNNNGG	5 cut(s)	744, 869, 1349, 1902, 2027
BseMI	GCAATG	3 cut(s)	761, 1096, 1154
BseMII	CTCAG	5 cut(s)	329, 519, 1353, 1995, 2066
BseNI	ACTGG	6 cut(s)	104, 504, 611, 697, 792, 1806
BseSI	GKGCMC	3 cut(s)	862, 926, 1425
BseXI	GCAGC	5 cut(s)	123, 317, 502, 691, 2035
BsgI	GTGCAG	2 cut(s)	57, 964
BshFI	GGCC	4 cut(s)	454, 779, 1562, 1906
BshNI	GGYRCC	2 cut(s)	859, 1067
BshVI	ATCGAT	2 cut(s)	1160, 1812
BsiHKAI	GWGCWC	2 cut(s)	926, 1425
BsiHKCI	CYCGRG	3 cut(s)	232, 1116, 1215
BsiSI	CCGG	4 cut(s)	108, 128, 776, 1238
BsiWI	CGTACG	1 cut(s)	1642
BslI	CCNNNNNNNGG	5 cut(s)	744, 869, 1349, 1902, 2027
BsmAI	GTCTC	1 cut(s)	1312
BsmI	GAATGC	1 cut(s)	1540
BsnI	GGCC	4 cut(s)	454, 779, 1562, 1906
BsoBI	CYCGRG	3 cut(s)	232, 1116, 1215
Bsp119I	TTCGAA	3 cut(s)	377, 1262, 1293
Bsp1286I	GDGCHC	5 cut(s)	862, 926, 945, 1111, 1425
Bsp13I	TCCGGA	1 cut(s)	1237
BspACI	CCGC	5 cut(s)	572, 1228, 1603, 1713, 2021
BspANI	GGCC	4 cut(s)	454, 779, 1562, 1906
BspCNI	CTCAG	5 cut(s)	328, 520, 1354, 1994, 2065
BspDI	ATCGAT	2 cut(s)	1160, 1812
BspEI	TCCGGA	1 cut(s)	1237
BspHI	TCATGA	1 cut(s)	217
BspLI	GGNNCC	2 cut(s)	861, 1069
BspMI	ACCTGC	2 cut(s)	871, 1112
BspOI	GCTAGC	1 cut(s)	1509
BspPI	GGATC	4 cut(s)	22, 901, 997, 1808
BspT104I	TTCGAA	3 cut(s)	377, 1262, 1293
BspT107I	GGYRCC	2 cut(s)	859, 1067
BsrDI	GCAATG	3 cut(s)	761, 1096, 1154
BsrFI	RCCGGY	1 cut(s)	107
BsrI	ACTGG	6 cut(s)	104, 504, 611, 697, 792, 1806
BssAI	RCCGGY	1 cut(s)	107
BssECI	CCNNGG	2 cut(s)	433, 455
BssNI	GRCGYC	1 cut(s)	1674
BssT1I	CCWWGG	2 cut(s)	433, 455
Bst2UI	CCWGG	2 cut(s)	1886, 2028
Bst4CI	ACNGT	2 cut(s)	985, 1493
Bst6I	CTCTTC	3 cut(s)	38, 620, 1715
BstACI	GRCGYC	1 cut(s)	1674
BstBAI	YACGTR	2 cut(s)	1627, 1642
BstBI	TTCGAA	3 cut(s)	377, 1262, 1293
BstC8I	GCNNGC	4 cut(s)	513, 781, 1507, 1862
BstDEI	CTNAG	8 cut(s)	315, 528, 1032, 1187, 1362, 1968, 1981, 2052
BstF5I	GGATG	1 cut(s)	1585
BstHHI	GCGC	2 cut(s)	258, 646
BstMAI	GTCTC	1 cut(s)	1312
BstMWI	GCNNNNNNNGC	4 cut(s)	704, 930, 1620, 1912
BstNI	CCWGG	2 cut(s)	1886, 2028
BstNSI	RCATGY	3 cut(s)	650, 1206, 1421
BstSCI	CCNGG	2 cut(s)	1884, 2026
BstSFI	CTRYAG	1 cut(s)	1678
BstSLI	GKGCMC	3 cut(s)	862, 926, 1425
BstSNI	TACGTA	2 cut(s)	1627, 1642
BstV1I	GCAGC	5 cut(s)	123, 317, 502, 691, 2035
BstV2I	GAAGAC	1 cut(s)	393
BstX2I	RGATCY	2 cut(s)	1028, 2055
BstXI	CCANNNNNNTGG	2 cut(s)	412, 787
BstYI	RGATCY	2 cut(s)	1028, 2055
Bsu15I	ATCGAT	2 cut(s)	1160, 1812
BsuRI	GGCC	4 cut(s)	454, 779, 1562, 1906
BsuTUI	ATCGAT	2 cut(s)	1160, 1812
BtsCI	GGATG	1 cut(s)	1585
BtsI	GCAGTG	1 cut(s)	1254
BtsIMutI	CAGTG	2 cut(s)	1254, 1359
BveI	ACCTGC	2 cut(s)	871, 1112
Cac8I	GCNNGC	4 cut(s)	513, 781, 1507, 1862
CciI	TCATGA	1 cut(s)	217
CfoI	GCGC	2 cut(s)	258, 646
Cfr10I	RCCGGY	1 cut(s)	107
Cfr13I	GGNCC	2 cut(s)	1235, 1739
ClaI	ATCGAT	2 cut(s)	1160, 1812
Csp6I	GTAC	7 cut(s)	103, 1068, 1628, 1643, 1669, 1725, 2016
CviAII	CATG	9 cut(s)	63, 218, 647, 1203, 1298, 1402, 1418, 1782, 1930
CviQI	GTAC	7 cut(s)	103, 1068, 1628, 1643, 1669, 1725, 2016
DdeI	CTNAG	8 cut(s)	315, 528, 1032, 1187, 1362, 1968, 1981, 2052
DraI	TTTAAA	2 cut(s)	678, 1500
EaeI	YGGCCR	2 cut(s)	777, 1560
Eam1104I	CTCTTC	3 cut(s)	38, 620, 1715
EarI	CTCTTC	3 cut(s)	38, 620, 1715
EciI	GGCGGA	1 cut(s)	1618
Eco105I	TACGTA	2 cut(s)	1627, 1642
Eco130I	CCWWGG	2 cut(s)	433, 455
Eco147I	AGGCCT	1 cut(s)	1906
Eco24I	GRGCYC	2 cut(s)	945, 1111
Eco47I	GGWCC	2 cut(s)	1235, 1739
Eco57I	CTGAAG	1 cut(s)	1268
Eco88I	CYCGRG	3 cut(s)	232, 1116, 1215
EcoRI	GAATTC	1 cut(s)	1289
EcoRII	CCWGG	2 cut(s)	1884, 2026
EcoT14I	CCWWGG	2 cut(s)	433, 455
EcoT22I	ATGCAT	1 cut(s)	1774
EcoT38I	GRGCYC	2 cut(s)	945, 1111
ErhI	CCWWGG	2 cut(s)	433, 455
FaeI	CATG	9 cut(s)	66, 221, 650, 1206, 1301, 1405, 1421, 1785, 1933
FatI	CATG	9 cut(s)	62, 217, 646, 1202, 1297, 1401, 1417, 1781, 1929
FauI	CCCGC	1 cut(s)	2028
FauNDI	CATATG	6 cut(s)	290, 1042, 1329, 1618, 1774, 2012
FbaI	TGATCA	2 cut(s)	480, 715
FblI	GTMKAC	2 cut(s)	1271, 1677
Fnu4HI	GCNGC	5 cut(s)	112, 306, 516, 705, 2024
FokI	GGATG	1 cut(s)	1592
FriOI	GRGCYC	2 cut(s)	945, 1111
Fsp4HI	GCNGC	5 cut(s)	112, 306, 516, 705, 2024
FspBI	CTAG	2 cut(s)	1337, 1506
GlaI	GCGC	2 cut(s)	257, 645
GluI	GCNGC	5 cut(s)	112, 306, 516, 705, 2024
HaeIII	GGCC	4 cut(s)	454, 779, 1562, 1906
HapII	CCGG	4 cut(s)	108, 128, 776, 1238
HhaI	GCGC	2 cut(s)	258, 646
Hin1I	GRCGYC	1 cut(s)	1674
Hin1II	CATG	9 cut(s)	66, 221, 650, 1206, 1301, 1405, 1421, 1785, 1933
Hin6I	GCGC	2 cut(s)	256, 644
HinP1I	GCGC	2 cut(s)	256, 644
HincII	GTYRAC	1 cut(s)	733
HindII	GTYRAC	1 cut(s)	733
HinfI	GANTC	7 cut(s)	85, 398, 596, 617, 1333, 1449, 1829
HpaI	GTTAAC	1 cut(s)	733
HpaII	CCGG	4 cut(s)	108, 128, 776, 1238
HphI	GGTGA	2 cut(s)	232, 1481
Hpy188III	TCNNGA	7 cut(s)	218, 232, 970, 1238, 1286, 1463, 1634
Hpy99I	CGWCG	2 cut(s)	1676, 1901
HpyAV	CCTTC	5 cut(s)	219, 384, 1360, 1427, 1583
HpyCH4III	ACNGT	2 cut(s)	985, 1493
HpyCH4IV	ACGT	5 cut(s)	421, 1268, 1626, 1641, 1674
HpyF10VI	GCNNNNNNNGC	4 cut(s)	704, 930, 1620, 1912
HpyF3I	CTNAG	8 cut(s)	315, 528, 1032, 1187, 1362, 1968, 1981, 2052
HpySE526I	ACGT	5 cut(s)	421, 1268, 1626, 1641, 1674
Hsp92I	GRCGYC	1 cut(s)	1674
Hsp92II	CATG	9 cut(s)	66, 221, 650, 1206, 1301, 1405, 1421, 1785, 1933
HspAI	GCGC	2 cut(s)	256, 644
Kpn2I	TCCGGA	1 cut(s)	1237
KpnI	GGTACC	1 cut(s)	1071
Ksp22I	TGATCA	2 cut(s)	480, 715
KspAI	GTTAAC	1 cut(s)	733
LmnI	GCTCC	1 cut(s)	285
Lsp1109I	GCAGC	5 cut(s)	123, 317, 502, 691, 2035
LweI	GCATC	3 cut(s)	875, 1847, 2038
MaeI	CTAG	2 cut(s)	1337, 1506
MaeII	ACGT	5 cut(s)	421, 1268, 1626, 1641, 1674
MaeIII	GTNAC	6 cut(s)	95, 520, 688, 880, 1438, 1818
MfeI	CAATTG	1 cut(s)	825
MflI	RGATCY	2 cut(s)	1028, 2055
MhlI	GDGCHC	5 cut(s)	862, 926, 945, 1111, 1425
MlsI	TGGCCA	1 cut(s)	1562
MluNI	TGGCCA	1 cut(s)	1562
MlyI	GAGTC	2 cut(s)	407, 1443
MmeI	TCCRAC	1 cut(s)	1223
Mox20I	TGGCCA	1 cut(s)	1562
Mph1103I	ATGCAT	1 cut(s)	1774
MroI	TCCGGA	1 cut(s)	1237
MroXI	GAANNNNTTC	1 cut(s)	2035
MscI	TGGCCA	1 cut(s)	1562
MseI	TTAA	9 cut(s)	462, 557, 677, 732, 761, 1002, 1305, 1499, 2043
MslI	CAYNNNNRTG	3 cut(s)	410, 813, 1734
Msp20I	TGGCCA	1 cut(s)	1562
MspI	CCGG	4 cut(s)	108, 128, 776, 1238
MspR9I	CCNGG	2 cut(s)	1886, 2028
MunI	CAATTG	1 cut(s)	825
Mva1269I	GAATGC	1 cut(s)	1540
MvaI	CCWGG	2 cut(s)	1886, 2028
MwoI	GCNNNNNNNGC	4 cut(s)	704, 930, 1620, 1912
NdeI	CATATG	6 cut(s)	290, 1042, 1329, 1618, 1774, 2012
NheI	GCTAGC	1 cut(s)	1505
NlaIII	CATG	9 cut(s)	66, 221, 650, 1206, 1301, 1405, 1421, 1785, 1933
NlaIV	GGNNCC	2 cut(s)	861, 1069
NmuCI	GTSAC	3 cut(s)	520, 880, 1818
NsiI	ATGCAT	1 cut(s)	1774
NspI	RCATGY	3 cut(s)	650, 1206, 1421
NspV	TTCGAA	3 cut(s)	377, 1262, 1293
PaeR7I	CTCGAG	2 cut(s)	232, 1215
PagI	TCATGA	1 cut(s)	217
PaqCI	CACCTGC	1 cut(s)	871
PceI	AGGCCT	1 cut(s)	1906
PciI	ACATGT	1 cut(s)	1202
PcsI	WCGNNNNNNNCGW	1 cut(s)	989
PctI	GAATGC	1 cut(s)	1540
PdmI	GAANNNNTTC	1 cut(s)	2035
PfeI	GAWTC	5 cut(s)	85, 596, 617, 1333, 1829
Pfl23II	CGTACG	1 cut(s)	1642
PfoI	TCCNGGA	1 cut(s)	1884
PkrI	GCNGC	5 cut(s)	113, 307, 517, 706, 2025
PleI	GAGTC	2 cut(s)	406, 1443
PpsI	GAGTC	2 cut(s)	406, 1443
Ppu21I	YACGTR	2 cut(s)	1627, 1642
PscI	ACATGT	1 cut(s)	1202
PshBI	ATTAAT	1 cut(s)	1002
Psp6I	CCWGG	2 cut(s)	1884, 2026
PspGI	CCWGG	2 cut(s)	1884, 2026
PspLI	CGTACG	1 cut(s)	1642
PspN4I	GGNNCC	2 cut(s)	861, 1069
PspPI	GGNCC	2 cut(s)	1235, 1739
PspXI	VCTCGAGB	1 cut(s)	1215
PsuI	RGATCY	2 cut(s)	1028, 2055
RsaI	GTAC	7 cut(s)	104, 1069, 1629, 1644, 1670, 1726, 2017
RsaNI	GTAC	7 cut(s)	103, 1068, 1628, 1643, 1669, 1725, 2016
RseI	CAYNNNNRTG	3 cut(s)	410, 813, 1734
SaqAI	TTAA	9 cut(s)	462, 557, 677, 732, 761, 1002, 1305, 1499, 2043
SatI	GCNGC	5 cut(s)	112, 306, 516, 705, 2024
Sau96I	GGNCC	2 cut(s)	1235, 1739
SchI	GAGTC	2 cut(s)	407, 1443
ScrFI	CCNGG	2 cut(s)	1886, 2028
SduI	GDGCHC	5 cut(s)	862, 926, 945, 1111, 1425
SfaNI	GCATC	3 cut(s)	875, 1847, 2038
SfcI	CTRYAG	1 cut(s)	1678
Sfr274I	CTCGAG	2 cut(s)	232, 1215
SfuI	TTCGAA	3 cut(s)	377, 1262, 1293
SinI	GGWCC	2 cut(s)	1235, 1739
SlaI	CTCGAG	2 cut(s)	232, 1215
SmiMI	CAYNNNNRTG	3 cut(s)	410, 813, 1734
SmlI	CTYRAG	5 cut(s)	232, 1215, 1275, 1443, 1947
SmoI	CTYRAG	5 cut(s)	232, 1215, 1275, 1443, 1947
SnaBI	TACGTA	2 cut(s)	1627, 1642
SseBI	AGGCCT	1 cut(s)	1906
SsiI	CCGC	5 cut(s)	572, 1228, 1603, 1713, 2021
SspMI	CTAG	2 cut(s)	1337, 1506
StuI	AGGCCT	1 cut(s)	1906
StyD4I	CCNGG	2 cut(s)	1884, 2026
StyI	CCWWGG	2 cut(s)	433, 455
TaaI	ACNGT	2 cut(s)	985, 1493
TaiI	ACGT	5 cut(s)	424, 1271, 1629, 1644, 1677
TatI	WGTACW	1 cut(s)	1724
TfiI	GAWTC	5 cut(s)	85, 596, 617, 1333, 1829
Tru1I	TTAA	9 cut(s)	462, 557, 677, 732, 761, 1002, 1305, 1499, 2043
Tru9I	TTAA	9 cut(s)	462, 557, 677, 732, 761, 1002, 1305, 1499, 2043
TscAI	CASTG	2 cut(s)	1254, 1366
TseFI	GTSAC	3 cut(s)	520, 880, 1818
TseI	GCWGC	5 cut(s)	111, 305, 515, 704, 2023
Tsp45I	GTSAC	3 cut(s)	520, 880, 1818
TspGWI	ACGGA	4 cut(s)	312, 951, 1732, 1806
TspRI	CASTG	2 cut(s)	1254, 1366
VneI	GTGCAC	2 cut(s)	922, 1421
VpaK11BI	GGWCC	2 cut(s)	1235, 1739
VspI	ATTAAT	1 cut(s)	1002
XapI	RAATTY	5 cut(s)	181, 631, 1101, 1289, 1805
XceI	RCATGY	3 cut(s)	650, 1206, 1421
XhoI	CTCGAG	2 cut(s)	232, 1215
XmiI	GTMKAC	2 cut(s)	1271, 1677
XmnI	GAANNNNTTC	1 cut(s)	2035
XspI	CTAG	2 cut(s)	1337, 1506
ZraI	GACGTC	1 cut(s)	1675
Zsp2I	ATGCAT	1 cut(s)	1774

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.