MD15G1156200.v1.1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000775
Physical Location & Seq
Forward (+)
11600506 .. 11604057
3552 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1156200.v1.1.491

Sequence Viewer

Length: 2307 bp
ATGGTATCCAAATTTTTAGTTCTACCATTCTTCTTCTTTTTCCAAATTTTGTTTGCTTTTAGATTCCAACCTTCGGAAGCACAATCTTGGATCAAAGTTGCATACTGGTATTCCGGCAGCGAATTCCCTATTTCCGACATCAACTCTCCGCTCTTCACTCACCTTATTTACGCTTACACAGATGTTAACTCATCATCCTATGAGCTCTCTTCCTCCTCCTCCGACTCCTCGGAGCAGTATTACTCCACCTTCACGCCAACCGTCAAGCGAAAGAACCCATCAGTCACTACTCTCGTCTCCATTGGCGGTGGAAACGCAGACTACGGTGTCATTTCAACCATGCTCAGTAGCTCTTCTCACAGAAAGTCTTTCGTTGATTCTTCAATAATTTTCGCAAGGCGTTATGGCTTCCTAGGCCTAGAATTTTGCTGGGCTTCAGCTAACACAAGTTCGGACATGACCAACATGGGGAAGTTATTTGAAGAGTGGCGGGCAGCTATCGCTTCTGAAGCCGCAAAAACCAGCCAAACACCACTGATTTTGACTGCTGCCGTGCTGTACAGGCCAGATTTTGAGTCCTTCGCATACCCGGTTGAATCAATCCGGGACAACTTGAACTGGGTACATGTCGTTTCCTATGCCTATTACGAACCTCAGCGGACCAATTTCACCGGTGCTCATGCAGCACTATACGATCCATCGAGTGACCTGAGCACCGACTATGGTACCAAATCATGGATTGGCAGGGGATTATCTGCTTCCAAATTGGTTTTGGGATTGCCTTCCTATGGATATGCTTGGACGCTAAGCAATCCCAGGGACAGCGCTATTGGCGCACCGGCTAAAGGTCCTGCCATTACCAAGGTTGGATCGATGAGCTACAAGGATATCAAAGGCTACATTGATAGATATGGGGCTGACATAGTCTACAATGCTACTTATGTAGCGAAGTACTGTGTGATCGGTTCGGCTTGGATCGGTTTCGATGATGCTGAAGTTGTGAAAATAAAAGTTTCTTATGCTAAGGAGCAGAAATTGCTTGGATACTTCATCTGGCAGGTCTCACTTGATGATAATTGGGTGCTTTCTCTTGCAGCTGCAGCTCAAGAGGGTGGAGGCAGTGGACAAAACAAACGGAGATTTCTTGTAATCGTTTTGACCACTATAGCTTCAGTTATTCTCTTGCTAGGCTCAGCGTTGAGTTACTTCCGCATGAGACTGCACAAATCAAAAGCTAAGGAATCAGAATCCAGGGAAAATGATATAGCAGACACAGCTGGAAAATTCAACAGCAACGTTCCTAATCTGAAAATATTTAGCATTGCTGACGTTGAGGCGGCAACGGATGGATTTTCGATTGAAAATAAGCTTGGAGAGGGCGGTTATGGCCCCGTTTATAAGGGAGTATTGCAAGATGGACAAGAAATCGCTGTGAAGAAACTATCAAAAGCTTCAACTCAAGGATTTGAGGAGTTCAAGAACGAGGTCATGCTCACCGCAAAACTACAACACGTAAATCTTGTTAGGGTTTTGGGATTTTGCATCGAACACCATGAGCAAATGCTGATTTACGAGTACATGCCAAAGAAAAGCTTGGACCTCTACCTCTTTGATCCTGTTAGAAGATATGAATTGGATTGGAGAAAGCGGGTTCAAATTATCAAAGGGGTTACTCAGGGACTTCTTTACCTCCAAGAGTACTCAAGATTGACAATCATCCATCGCGATCTGAAAGCTAGCAACATTTTACTTGATGAGGAGATGAGGCCTAAGATCTCGGATTTTGGTATGGCCAGAATTTTTGCAAAGGATGAACTCGAAGCAAATACAGGTCGAATTGTTGGAACGTATGGTTACGTACCACCGGAATACGTTCAGAAAGGTTTATACTCCACTAAATCAGATGTTTACAGCTTTGGAGTTCTCCTTCTACAGATCATAAGTGGCAAGAAGAATGCCCCCTACTATGGTTCAGATGAAGACTTAAACCTACTAGAATATGCATATTTACTTTGGAAACAAGGCAAAGGGATGGAGTTTATGGATCCTGCACTTGACGATACACATTCTTTATGCAAATTGATGAGATGCTTGCAAATTGCTCTCCTATGTGTTCAAGAAAATGCAAAAGATAGGCCTTCGATGTTGGAAATTTCTTCAATGCTACAAAATGAAGGAGCTACAATGGAAAATCCCAAAATTCCAGCCTTCTCAAAAAGAAATGAAGAGGAAGAAACAAATCCCATGCCGCGGCTAGAAGCTTGTTCCATCGATGATGCAACAATCTCTGAAGTTGTAGCCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18 Pkinase Pkinase_Tyr

Protein Analysis

769

Amino Acids

86.05

Weight (kDa)

5.95

Isoelectric Point (pI)

35.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 33 - 357 8.2e-61 Glycosyl hydrolases family 18
PK_Tyr_Ser-Thr PF07714 452 - 721 1.2e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 452 - 718 6.1e-44 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1398
AasI GACNNNNNNGTC 1 cut(s) 326
Acc36I ACCTGC 1 cut(s) 1048
Acc65I GGTACC 1 cut(s) 725
AccB1I GGYRCC 1 cut(s) 725
AccB7I CCANNNNNTGG 1 cut(s) 735
AccBSI CCGCTC 1 cut(s) 151
AccI GTMKAC 1 cut(s) 927
AccII CGCG 2 cut(s) 1725, 2251
AclI AACGTT 1 cut(s) 1296
AclWI GGATC 7 cut(s) 98, 689, 877, 983, 1607, 2039, 2052
AcoI YGGCCR 1 cut(s) 1791
AcsI RAATTY 8 cut(s) 11, 45, 122, 422, 1283, 1797, 2151, 2199
AcuI CTGAAG 4 cut(s) 420, 528, 1014, 1155
AfaI GTAC 7 cut(s) 560, 624, 727, 953, 1577, 1700, 1860
AfeI AGCGCT 1 cut(s) 826
AfiI CCNNNNNNNGG 7 cut(s) 73, 468, 735, 788, 845, 1967, 2250
AflIII ACRYGT 2 cut(s) 625, 1510
AgeI ACCGGT 1 cut(s) 671
AjnI CCWGG 2 cut(s) 815, 1250
AjuI GAANNNNNNNTTGG 2 cut(s) 1353, 1385
Alw21I GWGCWC 3 cut(s) 207, 679, 716
Alw26I GTCTC 3 cut(s) 301, 1066, 1210
AlwI GGATC 7 cut(s) 98, 689, 877, 983, 1607, 2039, 2052
Aor51HI AGCGCT 1 cut(s) 826
AoxI GGCC 6 cut(s) 415, 563, 1387, 1766, 1791, 2135
ApeKI GCWGC 7 cut(s) 117, 494, 548, 683, 1094, 1097, 1100
ApoI RAATTY 8 cut(s) 11, 45, 122, 422, 1283, 1797, 2151, 2199
AsiGI ACCGGT 1 cut(s) 671
Asp700I GAANNNNTTC 1 cut(s) 1872
Asp718I GGTACC 1 cut(s) 725
AspA2I CCTAGG 1 cut(s) 412
AspLEI GCGC 2 cut(s) 827, 836
AspS9I GGNCC 4 cut(s) 660, 848, 1388, 1597
AsuC2I CCSGG 2 cut(s) 590, 605
AsuHPI GGTGA 3 cut(s) 152, 661, 1486
AsuNHI GCTAGC 1 cut(s) 1736
AvaII GGWCC 3 cut(s) 660, 848, 1597
AvrII CCTAGG 1 cut(s) 412
BaeI ACNNNNGTAYC 2 cut(s) 709, 742
BalI TGGCCA 1 cut(s) 1793
BamHI GGATCC 1 cut(s) 2044
BanI GGYRCC 1 cut(s) 725
BanII GRGCYC 1 cut(s) 207
BbsI GAAGAC 1 cut(s) 1986
Bbv12I GWGCWC 3 cut(s) 207, 679, 716
BbvCI CCTCAGC 1 cut(s) 654
BbvI GCAGC 7 cut(s) 129, 506, 535, 695, 1084, 1106, 1112
BccI CCATC 7 cut(s) 286, 706, 1340, 1409, 1728, 2026, 2276
BceAI ACGGC 1 cut(s) 536
BciT130I CCWGG 2 cut(s) 817, 1252
BciVI GTATCC 2 cut(s) 16, 1037
BcnI CCSGG 2 cut(s) 590, 605
BcoDI GTCTC 3 cut(s) 301, 1066, 1210
BfaI CTAG 6 cut(s) 413, 419, 1187, 1737, 1994, 2255
BfmI CTRYAG 3 cut(s) 1098, 1164, 1931
BfoI RGCGCY 1 cut(s) 828
BfuAI ACCTGC 1 cut(s) 1048
BfuI GTATCC 2 cut(s) 16, 1037
BglII AGATCT 1 cut(s) 1773
BlnI CCTAGG 1 cut(s) 412
BlpI GCTNAGC 2 cut(s) 806, 1192
BmcAI AGTACT 2 cut(s) 953, 1700
Bme1390I CCNGG 4 cut(s) 590, 605, 817, 1252
Bme18I GGWCC 3 cut(s) 660, 848, 1597
BmgT120I GGNCC 4 cut(s) 660, 848, 1388, 1597
BmiI GGNNCC 3 cut(s) 727, 1390, 2046
BmrFI CCNGG 4 cut(s) 590, 605, 817, 1252
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 4 cut(s) 979, 1551, 2078, 2266
BmtI GCTAGC 1 cut(s) 1740
BmuI ACTGGG 1 cut(s) 628
BpiI GAAGAC 1 cut(s) 1986
Bpu10I CCTNAGC 4 cut(s) 654, 710, 1023, 1236
Bpu1102I GCTNAGC 2 cut(s) 806, 1192
BpuEI CTTGAG 3 cut(s) 1089, 1443, 1687
BpuMI CCSGG 2 cut(s) 590, 605
Bsa29I ATCGAT 2 cut(s) 872, 2271
BsaAI YACGTR 2 cut(s) 1513, 1858
BsaI GGTCTC 1 cut(s) 1066
BsaJI CCNNGG 7 cut(s) 228, 412, 815, 816, 861, 1251, 2249
BsaWI WCCGGW 2 cut(s) 671, 1864
Bsc4I CCNNNNNNNGG 7 cut(s) 73, 468, 735, 788, 845, 1967, 2250
Bse118I RCCGGY 2 cut(s) 671, 838
Bse1I ACTGG 2 cut(s) 110, 623
Bse3DI GCAATG 1 cut(s) 1320
BseBI CCWGG 2 cut(s) 817, 1252
BseCI ATCGAT 2 cut(s) 872, 2271
BseDI CCNNGG 7 cut(s) 228, 412, 815, 816, 861, 1251, 2249
BseGI GGATG 5 cut(s) 194, 1351, 1716, 1816, 2037
BseLI CCNNNNNNNGG 7 cut(s) 73, 468, 735, 788, 845, 1967, 2250
BseMI GCAATG 1 cut(s) 1320
BseMII CTCAG 5 cut(s) 358, 668, 701, 1206, 1688
BseNI ACTGG 2 cut(s) 110, 623
BseRI GAGGAG 5 cut(s) 205, 208, 217, 1484, 1772
BseXI GCAGC 7 cut(s) 129, 506, 535, 695, 1084, 1106, 1112
BseYI CCCAGC 1 cut(s) 429
BsgI GTGCAG 2 cut(s) 1205, 2034
Bsh1236I CGCG 2 cut(s) 1725, 2251
BshFI GGCC 6 cut(s) 417, 565, 1389, 1768, 1793, 2137
BshNI GGYRCC 1 cut(s) 725
BshTI ACCGGT 1 cut(s) 671
BshVI ATCGAT 2 cut(s) 872, 2271
BsiHKAI GWGCWC 3 cut(s) 207, 679, 716
BsiSI CCGG 6 cut(s) 114, 590, 604, 672, 839, 1865
BslFI GGGAC 3 cut(s) 620, 833, 1692
BslI CCNNNNNNNGG 7 cut(s) 73, 468, 735, 788, 845, 1967, 2250
BsmAI GTCTC 3 cut(s) 301, 1066, 1210
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 3 cut(s) 620, 833, 1692
BsmI GAATGC 1 cut(s) 1960
BsnI GGCC 6 cut(s) 417, 565, 1389, 1768, 1793, 2137
Bso31I GGTCTC 1 cut(s) 1066
Bsp1286I GDGCHC 3 cut(s) 207, 679, 716
Bsp1407I TGTACA 1 cut(s) 558
Bsp1720I GCTNAGC 2 cut(s) 806, 1192
Bsp68I TCGCGA 1 cut(s) 1725
BspANI GGCC 6 cut(s) 417, 565, 1389, 1768, 1793, 2137
BspCNI CTCAG 5 cut(s) 357, 667, 702, 1205, 1687
BspDI ATCGAT 2 cut(s) 872, 2271
BspFNI CGCG 2 cut(s) 1725, 2251
BspLI GGNNCC 3 cut(s) 727, 1390, 2046
BspMAI CTGCAG 1 cut(s) 1102
BspMI ACCTGC 1 cut(s) 1048
BspOI GCTAGC 1 cut(s) 1740
BspPI GGATC 7 cut(s) 98, 689, 877, 983, 1607, 2039, 2052
BspQI GCTCTTC 2 cut(s) 158, 358
BspT107I GGYRCC 1 cut(s) 725
BspTNI GGTCTC 1 cut(s) 1066
BsrBI CCGCTC 1 cut(s) 151
BsrDI GCAATG 1 cut(s) 1320
BsrFI RCCGGY 2 cut(s) 671, 838
BsrGI TGTACA 1 cut(s) 558
BsrI ACTGG 2 cut(s) 110, 623
BssAI RCCGGY 2 cut(s) 671, 838
BssECI CCNNGG 7 cut(s) 228, 412, 815, 816, 861, 1251, 2249
BssT1I CCWWGG 2 cut(s) 412, 861
Bst2UI CCWGG 2 cut(s) 817, 1252
Bst4CI ACNGT 3 cut(s) 262, 326, 956
Bst6I CTCTTC 5 cut(s) 158, 214, 358, 477, 2220
BstAPI GCANNNNNTGC 1 cut(s) 1036
BstAUI TGTACA 1 cut(s) 558
BstBAI YACGTR 2 cut(s) 1513, 1858
BstC8I GCNNGC 3 cut(s) 492, 1738, 2093
BstDEI CTNAG 9 cut(s) 344, 654, 710, 806, 1023, 1192, 1236, 1674, 1770
BstDSI CCRYGG 1 cut(s) 2249
BstF5I GGATG 5 cut(s) 194, 1351, 1716, 1816, 2037
BstFNI CGCG 2 cut(s) 1725, 2251
BstH2I RGCGCY 1 cut(s) 828
BstHHI GCGC 2 cut(s) 827, 836
BstMAI GTCTC 3 cut(s) 301, 1066, 1210
BstNI CCWGG 2 cut(s) 817, 1252
BstNSI RCATGY 2 cut(s) 629, 1582
BstSCI CCNGG 4 cut(s) 588, 603, 815, 1250
BstSFI CTRYAG 3 cut(s) 1098, 1164, 1931
BstSNI TACGTA 1 cut(s) 1858
BstUI CGCG 2 cut(s) 1725, 2251
BstV1I GCAGC 7 cut(s) 129, 506, 535, 695, 1084, 1106, 1112
BstV2I GAAGAC 1 cut(s) 1986
BstX2I RGATCY 2 cut(s) 1773, 2044
BstYI RGATCY 2 cut(s) 1773, 2044
Bsu15I ATCGAT 2 cut(s) 872, 2271
BsuI GTATCC 2 cut(s) 16, 1037
BsuRI GGCC 6 cut(s) 417, 565, 1389, 1768, 1793, 2137
BsuTUI ATCGAT 2 cut(s) 872, 2271
BtgI CCRYGG 1 cut(s) 2249
BtgZI GCGATG 1 cut(s) 1706
BtsCI GGATG 5 cut(s) 194, 1351, 1716, 1816, 2037
BtsI GCAGTG 1 cut(s) 1126
BtsIMutI CAGTG 2 cut(s) 533, 1126
BtuMI TCGCGA 1 cut(s) 1725
BveI ACCTGC 1 cut(s) 1048
Cac8I GCNNGC 3 cut(s) 492, 1738, 2093
CfoI GCGC 2 cut(s) 827, 836
Cfr10I RCCGGY 2 cut(s) 671, 838
Cfr13I GGNCC 4 cut(s) 660, 848, 1388, 1597
Cfr42I CCGCGG 1 cut(s) 2252
ClaI ATCGAT 2 cut(s) 872, 2271
CseI GACGC 1 cut(s) 811
Csp6I GTAC 7 cut(s) 559, 623, 726, 952, 1576, 1699, 1859
CspAI ACCGGT 1 cut(s) 671
CspCI CAANNNNNGTGG 2 cut(s) 522, 557
CviQI GTAC 7 cut(s) 559, 623, 726, 952, 1576, 1699, 1859
DdeI CTNAG 9 cut(s) 344, 654, 710, 806, 1023, 1192, 1236, 1674, 1770
DrdI GACNNNNNNGTC 1 cut(s) 326
DseDI GACNNNNNNGTC 1 cut(s) 326
EaeI YGGCCR 1 cut(s) 1791
Eam1104I CTCTTC 5 cut(s) 158, 214, 358, 477, 2220
EarI CTCTTC 5 cut(s) 158, 214, 358, 477, 2220
Ecl136II GAGCTC 1 cut(s) 205
Eco105I TACGTA 1 cut(s) 1858
Eco130I CCWWGG 2 cut(s) 412, 861
Eco147I AGGCCT 3 cut(s) 417, 1768, 2137
Eco24I GRGCYC 1 cut(s) 207
Eco31I GGTCTC 1 cut(s) 1066
Eco32I GATATC 1 cut(s) 889
Eco47I GGWCC 3 cut(s) 660, 848, 1597
Eco47III AGCGCT 1 cut(s) 826
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 4 cut(s) 420, 528, 1014, 1155
EcoICRI GAGCTC 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 848
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 815, 1250
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 2 cut(s) 412, 861
EcoT22I ATGCAT 1 cut(s) 2005
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 2 cut(s) 412, 861
Esp3I CGTCTC 1 cut(s) 301
FalI AAGNNNNNCTT 2 cut(s) 1577, 1609
FaqI GGGAC 3 cut(s) 620, 833, 1692
FauI CCCGC 2 cut(s) 483, 1641
FblI GTMKAC 1 cut(s) 927
FokI GGATG 5 cut(s) 181, 1358, 1703, 1823, 2044
FriOI GRGCYC 1 cut(s) 207
FspBI CTAG 6 cut(s) 413, 419, 1187, 1737, 1994, 2255
GlaI GCGC 2 cut(s) 826, 835
GsaI CCCAGC 1 cut(s) 433
HaeII RGCGCY 1 cut(s) 828
HaeIII GGCC 6 cut(s) 417, 565, 1389, 1768, 1793, 2137
HapII CCGG 6 cut(s) 114, 590, 604, 672, 839, 1865
HgaI GACGC 1 cut(s) 811
HhaI GCGC 2 cut(s) 827, 836
Hin6I GCGC 2 cut(s) 825, 834
HinP1I GCGC 2 cut(s) 825, 834
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HindIII AAGCTT 4 cut(s) 1367, 1449, 1591, 2259
HinfI GANTC 7 cut(s) 63, 224, 377, 575, 596, 1241, 1247
HpaI GTTAAC 1 cut(s) 187
HpaII CCGG 6 cut(s) 114, 590, 604, 672, 839, 1865
HphI GGTGA 3 cut(s) 152, 661, 1486
Hpy166II GTNNAC 4 cut(s) 187, 928, 1124, 1909
Hpy188III TCNNGA 5 cut(s) 1106, 1477, 1704, 1724, 2117
Hpy8I GTNNAC 4 cut(s) 187, 928, 1124, 1909
HpyAV CCTTC 8 cut(s) 81, 259, 589, 792, 1937, 2148, 2168, 2218
HpyCH4III ACNGT 3 cut(s) 262, 326, 956
HpyCH4IV ACGT 6 cut(s) 1296, 1329, 1512, 1847, 1857, 1872
HpyF3I CTNAG 9 cut(s) 344, 654, 710, 806, 1023, 1192, 1236, 1674, 1770
HpySE526I ACGT 6 cut(s) 1296, 1329, 1512, 1847, 1857, 1872
HspAI GCGC 2 cut(s) 825, 834
KpnI GGTACC 1 cut(s) 729
KspAI GTTAAC 1 cut(s) 187
KspI CCGCGG 1 cut(s) 2252
LguI GCTCTTC 2 cut(s) 158, 358
LmnI GCTCC 3 cut(s) 232, 1027, 2177
Lsp1109I GCAGC 7 cut(s) 129, 506, 535, 695, 1084, 1106, 1112
LweI GCATC 4 cut(s) 979, 1551, 2078, 2266
MaeI CTAG 6 cut(s) 413, 419, 1187, 1737, 1994, 2255
MaeII ACGT 6 cut(s) 1296, 1329, 1512, 1847, 1857, 1872
MaeIII GTNAC 5 cut(s) 283, 704, 1202, 1669, 1853
MbiI CCGCTC 1 cut(s) 151
MflI RGATCY 2 cut(s) 1773, 2044
MhlI GDGCHC 3 cut(s) 207, 679, 716
MlsI TGGCCA 1 cut(s) 1793
MluNI TGGCCA 1 cut(s) 1793
MlyI GAGTC 2 cut(s) 218, 584
MmeI TCCRAC 6 cut(s) 91, 159, 246, 847, 1822, 2127
Mox20I TGGCCA 1 cut(s) 1793
Mph1103I ATGCAT 1 cut(s) 2005
MroXI GAANNNNTTC 1 cut(s) 1872
MscI TGGCCA 1 cut(s) 1793
MseI TTAA 2 cut(s) 186, 1985
Msp20I TGGCCA 1 cut(s) 1793
MspA1I CMGCKG 4 cut(s) 658, 1097, 1277, 2251
MspI CCGG 6 cut(s) 114, 590, 604, 672, 839, 1865
MspR9I CCNGG 4 cut(s) 590, 605, 817, 1252
Mva1269I GAATGC 1 cut(s) 1960
MvaI CCWGG 2 cut(s) 817, 1252
MvnI CGCG 2 cut(s) 1725, 2251
NciI CCSGG 2 cut(s) 590, 605
NheI GCTAGC 1 cut(s) 1736
NlaIV GGNNCC 3 cut(s) 727, 1390, 2046
NmuCI GTSAC 2 cut(s) 283, 704
NruI TCGCGA 1 cut(s) 1725
NsiI ATGCAT 1 cut(s) 2005
NspI RCATGY 2 cut(s) 629, 1582
PasI CCCWGGG 1 cut(s) 816
PceI AGGCCT 3 cut(s) 417, 1768, 2137
PciI ACATGT 1 cut(s) 625
PciSI GCTCTTC 2 cut(s) 158, 358
PctI GAATGC 1 cut(s) 1960
PdmI GAANNNNTTC 1 cut(s) 1872
PfeI GAWTC 5 cut(s) 63, 377, 596, 1241, 1247
PflFI GACNNNGTC 1 cut(s) 923
PflMI CCANNNNNTGG 1 cut(s) 735
PfoI TCCNGGA 1 cut(s) 603
PinAI ACCGGT 1 cut(s) 671
PleI GAGTC 2 cut(s) 218, 583
PpsI GAGTC 2 cut(s) 218, 583
Ppu21I YACGTR 2 cut(s) 1513, 1858
PpuMI RGGWCCY 1 cut(s) 848
PscI ACATGT 1 cut(s) 625
PsiI TTATAA 1 cut(s) 1398
Psp124BI GAGCTC 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 1296
Psp5II RGGWCCY 1 cut(s) 848
Psp6I CCWGG 2 cut(s) 815, 1250
PspFI CCCAGC 1 cut(s) 429
PspGI CCWGG 2 cut(s) 815, 1250
PspN4I GGNNCC 3 cut(s) 727, 1390, 2046
PspPI GGNCC 4 cut(s) 660, 848, 1388, 1597
PspPPI RGGWCCY 1 cut(s) 848
PstI CTGCAG 1 cut(s) 1102
PsuI RGATCY 2 cut(s) 1773, 2044
PsyI GACNNNGTC 1 cut(s) 923
PvuII CAGCTG 2 cut(s) 1097, 1277
RruI TCGCGA 1 cut(s) 1725
RsaI GTAC 7 cut(s) 560, 624, 727, 953, 1577, 1700, 1860
RsaNI GTAC 7 cut(s) 559, 623, 726, 952, 1576, 1699, 1859
SacI GAGCTC 1 cut(s) 207
SacII CCGCGG 1 cut(s) 2252
SapI GCTCTTC 2 cut(s) 158, 358
SaqAI TTAA 2 cut(s) 186, 1985
Sau96I GGNCC 4 cut(s) 660, 848, 1388, 1597
ScaI AGTACT 2 cut(s) 953, 1700
SchI GAGTC 2 cut(s) 218, 584
ScrFI CCNGG 4 cut(s) 590, 605, 817, 1252
SduI GDGCHC 3 cut(s) 207, 679, 716
SfaNI GCATC 4 cut(s) 979, 1551, 2078, 2266
SfcI CTRYAG 3 cut(s) 1098, 1164, 1931
Sfr303I CCGCGG 1 cut(s) 2252
SgrAI CRCCGGYG 1 cut(s) 671
SgrBI CCGCGG 1 cut(s) 2252
SinI GGWCC 3 cut(s) 660, 848, 1597
SmlI CTYRAG 3 cut(s) 1104, 1458, 1702
SmoI CTYRAG 3 cut(s) 1104, 1458, 1702
SnaBI TACGTA 1 cut(s) 1858
SseBI AGGCCT 3 cut(s) 417, 1768, 2137
SspI AATATT 1 cut(s) 1314
SspMI CTAG 6 cut(s) 413, 419, 1187, 1737, 1994, 2255
SstI GAGCTC 1 cut(s) 207
StuI AGGCCT 3 cut(s) 417, 1768, 2137
StyD4I CCNGG 4 cut(s) 588, 603, 815, 1250
StyI CCWWGG 2 cut(s) 412, 861
TaaI ACNGT 3 cut(s) 262, 326, 956
TaiI ACGT 6 cut(s) 1299, 1332, 1515, 1850, 1860, 1875
TaqI TCGA 9 cut(s) 701, 872, 984, 1355, 1545, 1818, 1834, 2141, 2271
TatI WGTACW 4 cut(s) 558, 951, 1575, 1698
TauI GCSGC 4 cut(s) 515, 1340, 2251, 2254
TfiI GAWTC 5 cut(s) 63, 377, 596, 1241, 1247
Tru1I TTAA 2 cut(s) 186, 1985
Tru9I TTAA 2 cut(s) 186, 1985
TscAI CASTG 2 cut(s) 540, 1126
TseFI GTSAC 2 cut(s) 283, 704
TseI GCWGC 7 cut(s) 117, 494, 548, 683, 1094, 1097, 1100
Tsp45I GTSAC 2 cut(s) 283, 704
TspDTI ATGAA 6 cut(s) 1039, 1644, 1827, 1992, 2187, 2238
TspGWI ACGGA 2 cut(s) 1150, 1358
TspRI CASTG 2 cut(s) 540, 1126
Tth111I GACNNNGTC 1 cut(s) 923
Van91I CCANNNNNTGG 1 cut(s) 735
VpaK11BI GGWCC 3 cut(s) 660, 848, 1597
XapI RAATTY 8 cut(s) 11, 45, 122, 422, 1283, 1797, 2151, 2199
XceI RCATGY 2 cut(s) 629, 1582
XcmI CCANNNNNNNNNTGG 1 cut(s) 769
XmaJI CCTAGG 1 cut(s) 412
XmiI GTMKAC 1 cut(s) 927
XmnI GAANNNNTTC 1 cut(s) 1872
XspI CTAG 6 cut(s) 413, 419, 1187, 1737, 1994, 2255
ZrmI AGTACT 2 cut(s) 953, 1700
Zsp2I ATGCAT 1 cut(s) 2005
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.