Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0000775

Physical Location & Seq

Genomic Coordinates Forward (+)

11661076 .. 11664251

Gene Structure 3176 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1157000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGGTATCGAAAAATATCTCCCACCCGATCCTCTTCATCTTCCTTGTTTCTTGTAATTTTTCGTGTTCTTCCGCACAAACTTGGATTCGAGCTGGTTACTGGTACACCGGCAGCGACTTTCCTTTACAGGAGGTAAACTCTGCCTTGTTCACACATCTTATTTGTTCTTTTGCCAATGTGAATTTCTCCACCTACCAGCTCTCCATCCCTTCTGCTCATGAACAATACTTCTCGAGCTTCACCGATATTGTCAAGCGCAAAAACCCATCAATCACAACGCTCCTATCCATATGGGATGGAGAAGCAGCAACAACTCAGCTAATTTTGGGTCAGAGAGCAAACTTTTCAGTCTTGTCTAAAATGCTCGAAATCCCTTCGAATAGAAAGTCTTTCATTGAGTCCTCCATCGAAGTGGCAAAACGTTATGGCTTTCAAGGTATAGACTTACTCTGGGCTTGGCTTAACACAACCTCAGATGTGATCAATATGGAAAAGCTACTGGACGAGTGGCGAGTTGCTGTGATTTCTGAGGCAAGAAACTCTGGTCTGCCACGATTAACATTGACAATGGCGGTTAGGTATATTCCCACTTTTGAATCGTTGATTTATCCGGTTGAATCTATGAAGAGGAATTTGGATTGGGCGCATGTTGTGGCGTATGACTACCATCTGCCTTTAAAGGAGAATGTTATTGGTGCTCATGCTGCTTTATATGATCCTTCGAGCTATGTTAACACCGACTATGGGATAAAGCAATGGTTAAACTACTCATTTCCGGCCAGCAAACTGGTTTTGGGTTTGCCTTACCATGGCTATGCGTGGACAGTTGCAAACCCCAAAGACAATAATGGAATCGGGGCACCTGCATCTGGCTTTGCTGTGACAAAAGATGGATCAATGAGCTATAAGCTCATCAAGTGGTACATTCGAAGCTACAGAGGGCTCATAGCGTATAATGATACTTATGTCTTGAATTACTGCATGGTAGGATCGACTTGGATTAATTTCGATGATGCGGAAGCCATCAGAGCTAAGATTGCATATGTGAAAGCGAAGAAGCTACTCGGTACCAATATGTTTCAAGTCAGCAATGATGATGAAAATTGGGCACTTTCTCGGGCAGCTCAGGAGGAAGGAAATGATCACGAAAAGAAACCCCGGTTACTGCTTGTAATAGTGCTATCAGTTACTTCGGTCGTAATTCTCATAGCTTTCGTGGTGTGGATGATTGTTCTAGGCAGATCAAGGTCTACTAGTACATCAGCTGTTGACAGTGATGCTCCGAATCTGAAAGTATACCACTTTTCCAGTCTCAAAGCGGCTACAAATAACTTCTCAATTGAAAACAAACTTGGAGAGGGTGGATTTGGTCCCCGTTTACAAGGTCAGGAAATAGCAGTGAAAAGGCTTTCAAAAACGTCTACTCAAGGACTTCAGGAATTCGAAAATGAGGTTAAACTTACTGCGAGACTACAACATGTGAATCTAGTTCCTGTTCTTGGATTTTGCACTGAGAATGAAGAAAAAATGCTGATTCACGAGTACATGCCCAACAAAAGCTTGGACTTCTACCTTTTCGATCCTACCGGACAGTATTTCATGGATTGGAGAAAGCGTGTGCACATCATCGAAGGCATTATTCAAGAACTCCTATATCTTCAAGAATACTCAAACTTCACAATCATTCACCGTGATCTAAAAGCTAGCAACATTTTGCATGATGACGAGATGAATGCTAAGATTTCCGATTTTGGAATGGCCAAACTTTTCAGAAAGGATGAACTCGAAGGAAACACAAGGCGGATTGTTGGCACATATGGCTACGTACCTCCTGAATACATACGAAAAGGCATATACTCTATGAAGTACGACGTCTACAGCTTCGGAGTTCTACTTTTGCAAATGATTAGCGGACGGAAGAGTACACATTACTATGGTCCGAACGAAAGTATGCACCTCTTGGAATATGCATATGTATCATGGAAAGAGGACAAAGGACTGGATTTTATCGATCCGTCACTTGACGATTCTTCTTCTTCTTGTAAGTTATTGAGATGCTTGCAAGTGGCACTGCTCTGTGTCCAGGAAAATGCCGACGATAGGCCTACAATGCTGGAAGTTTATTCCATGCTCATATCTGACACTCAAGCCGTTCCAACTCCTACAAAGACGGCTTTCTCAGTGAAAAATTACGACAACGTGGACAACATATGTACCCCGCAGCCAGGAAATTGTTCGGTTAATGATGCTCAGATCTCCGAACTCCAACCCCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

Glyco_hydro_18 Pkinase Pkinase_Tyr

Protein Analysis

759

Amino Acids

85.96

Weight (kDa)

6.19

Isoelectric Point (pI)

38.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_18	PF00704	32 - 365	5.5e-53	Glycosyl hydrolases family 18
Pkinase	PF00069	446 - 707	2.6e-37	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	449 - 710	8.7e-36	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000775)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g01350 FvH4_1g01363 FvH4_1g01365 FvH4_1g01383 FvH4_1g01390 FvH4_1g01420
malus_domestica	MD02G1011200.v1.1 MD02G1011400.v1.1 MD15G1156200.v1.1 MD15G1156300.v1.1 MD15G1156600.v1.1 MD15G1156700.v1.1 MD15G1156900.v1.1 MD15G1157000.v1.1
prunus_persica	Prupe.7G258600_v2.0.a1 Prupe.7G258700_v2.0.a1 Prupe.7G258700_v2.0.a1 Prupe.7G258800_v2.0.a1 Prupe.7G258900_v2.0.a1
pyrus_communis	pycom02g01010 pycom02g01020 pycom14g14440 pycom15g14020 pycom15g14030 pycom15g14050 pycom15g14060 pycom15g14080
rosa_chinensis	RchiOBHm_Chr2g0086191 RchiOBHm_Chr2g0086211 RchiOBHm_Chr2g0086231 RchiOBHm_Chr2g0086261 RchiOBHm_Chr2g0086281 RchiOBHm_Chr2g0086301 RchiOBHm_Chr2g0086311 RchiOBHm_Chr2g0086321 RchiOBHm_Chr2g0160801 RchiOBHm_Chr6g0247981 RchiOBHm_Chr6g0247991
rosa_laevigata	RLG00000015749
rosa_multiflora	Rmu_co8497771.1_g000001 Rmu_sc0004510.1_g000014 Rmu_sc0004510.1_g000018 Rmu_sc0004948.1_g000005 Rmu_sc0004948.1_g000017 Rmu_sc0004948.1_g000018 Rmu_sc0004948.1_g000024
rosa_roxburghii	Rroxscaffold_2G00154700 Rroxscaffold_2G00154710
rosa_rugosa	Rorug01G0466400 Rorug01G0466500 Rorug05G0529900
rosa_samantha	Rh2AG016200 Rh2AG016300 Rh2AG016500 Rh2AG016700 Rh2BG016300 Rh2BG016400 Rh2BG016500 Rh2CG017200 Rh2CG017300 Rh2CG017400 Rh2CG017500 Rh2CG017600 Rh2DG018200 Rh2DG018300 Rh6AG044800 Rh6BG039800
rosa_wichuraiana	Rw0G015400 Rw2G001400 Rw2G001410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	871
AatII	GACGTC	1 cut(s)	1875
Acc36I	ACCTGC	1 cut(s)	871
Acc65I	GGTACC	1 cut(s)	1067
AccB1I	GGYRCC	2 cut(s)	859, 1067
AccI	GTMKAC	4 cut(s)	1250, 1296, 1421, 1875
AciI	CCGC	7 cut(s)	72, 572, 1016, 1319, 1801, 1911, 2219
AclI	AACGTT	1 cut(s)	421
AclWI	GGATC	6 cut(s)	22, 710, 901, 997, 1574, 2006
AcoI	YGGCCR	2 cut(s)	777, 1758
AcsI	RAATTY	3 cut(s)	181, 631, 1439
AcuI	CTGAAG	1 cut(s)	1418
AcyI	GRCGYC	1 cut(s)	1872
AfaI	GTAC	9 cut(s)	104, 923, 1069, 1258, 1544, 1827, 1868, 1924, 2215
AfiI	CCNNNNNNNGG	6 cut(s)	744, 809, 869, 1499, 2100, 2225
AflIII	ACRYGT	1 cut(s)	1477
AgsI	TTSAA	9 cut(s)	434, 596, 617, 973, 1082, 1343, 1413, 1643, 1661
AhlI	ACTAGT	1 cut(s)	1253
AjnI	CCWGG	2 cut(s)	2082, 2224
AjuI	GAANNNNNNNTTGG	6 cut(s)	167, 199, 617, 649, 1335, 1367
Alw21I	GWGCWC	2 cut(s)	700, 1623
Alw26I	GTCTC	2 cut(s)	1316, 1462
Alw44I	GTGCAC	1 cut(s)	1619
AlwI	GGATC	6 cut(s)	22, 710, 901, 997, 1574, 2006
Ama87I	CYCGRG	2 cut(s)	232, 1116
AoxI	GGCC	3 cut(s)	777, 1758, 2102
ApaLI	GTGCAC	1 cut(s)	1619
ApeKI	GCWGC	5 cut(s)	111, 305, 704, 1121, 2221
ApoI	RAATTY	3 cut(s)	181, 631, 1439
ArsI	GACNNNNNNTTYG	2 cut(s)	371, 403
AseI	ATTAAT	1 cut(s)	1002
Asp700I	GAANNNNTTC	1 cut(s)	2233
Asp718I	GGTACC	1 cut(s)	1067
AspLEI	GCGC	2 cut(s)	258, 646
AspS9I	GGNCC	2 cut(s)	1370, 1937
AsuC2I	CCSGG	1 cut(s)	1159
AsuHPI	GGTGA	2 cut(s)	232, 1679
AsuII	TTCGAA	3 cut(s)	377, 928, 1443
AsuNHI	GCTAGC	1 cut(s)	1703
AvaI	CYCGRG	2 cut(s)	232, 1116
AvaII	GGWCC	2 cut(s)	1370, 1937
BaeGI	GKGCMC	3 cut(s)	862, 1111, 1623
BaeI	ACNNNNGTAYC	4 cut(s)	951, 984, 2197, 2230
BalI	TGGCCA	1 cut(s)	1760
BanI	GGYRCC	2 cut(s)	859, 1067
BanII	GRGCYC	1 cut(s)	945
BauI	CACGAG	1 cut(s)	1538
Bbv12I	GWGCWC	2 cut(s)	700, 1623
BbvI	GCAGC	5 cut(s)	123, 317, 691, 1133, 2233
BccI	CCATC	7 cut(s)	212, 274, 290, 413, 675, 884, 1031
BceAI	ACGGC	2 cut(s)	2135, 2187
BcgI	CGANNNNNNTGC	2 cut(s)	1715, 1749
BciT130I	CCWGG	2 cut(s)	2084, 2226
BclI	TGATCA	2 cut(s)	480, 1141
BcnI	CCSGG	1 cut(s)	1159
BcoDI	GTCTC	2 cut(s)	1316, 1462
BcuI	ACTAGT	1 cut(s)	1253
BfaI	CTAG	4 cut(s)	1235, 1254, 1487, 1704
BfmI	CTRYAG	2 cut(s)	934, 1876
BfuAI	ACCTGC	1 cut(s)	871
BglII	AGATCT	1 cut(s)	2253
BisI	GCNGC	6 cut(s)	112, 306, 705, 1122, 1320, 2222
BlsI	GCNGC	6 cut(s)	113, 307, 706, 1123, 1321, 2223
Bme1390I	CCNGG	3 cut(s)	1159, 2084, 2226
Bme18I	GGWCC	2 cut(s)	1370, 1937
BmeT110I	CYCGRG	2 cut(s)	232, 1116
BmgT120I	GGNCC	2 cut(s)	1370, 1937
BmiI	GGNNCC	3 cut(s)	861, 1069, 1372
BmrFI	CCNGG	3 cut(s)	1159, 2084, 2226
BmsI	GCATC	5 cut(s)	875, 1003, 1267, 2045, 2236
BmtI	GCTAGC	1 cut(s)	1707
BplI	GAGNNNNNCTC	2 cut(s)	122, 154
Bpu10I	CCTNAGC	1 cut(s)	1125
Bpu14I	TTCGAA	3 cut(s)	377, 928, 1443
BpuEI	CTTGAG	2 cut(s)	1410, 2130
BpuMI	CCSGG	1 cut(s)	1159
Bsa29I	ATCGAT	1 cut(s)	2010
BsaAI	YACGTR	1 cut(s)	1825
BsaHI	GRCGYC	1 cut(s)	1872
BsaJI	CCNNGG	2 cut(s)	808, 1157
BsaWI	WCCGGW	2 cut(s)	610, 1586
BsaXI	ACNNNNNCTCC	4 cut(s)	185, 215, 1600, 1630
Bsc4I	CCNNNNNNNGG	6 cut(s)	744, 809, 869, 1499, 2100, 2225
Bse118I	RCCGGY	1 cut(s)	107
Bse1I	ACTGG	5 cut(s)	104, 504, 792, 1308, 2004
Bse3DI	GCAATG	2 cut(s)	761, 1096
BseBI	CCWGG	2 cut(s)	2084, 2226
BseCI	ATCGAT	1 cut(s)	2010
BseDI	CCNNGG	2 cut(s)	808, 1157
BseGI	GGATG	4 cut(s)	204, 301, 1230, 1783
BseLI	CCNNNNNNNGG	6 cut(s)	744, 809, 869, 1499, 2100, 2225
BseMI	GCAATG	2 cut(s)	761, 1096
BseMII	CTCAG	7 cut(s)	329, 486, 519, 1139, 1503, 2193, 2264
BseNI	ACTGG	5 cut(s)	104, 504, 792, 1308, 2004
BseSI	GKGCMC	3 cut(s)	862, 1111, 1623
BseXI	GCAGC	5 cut(s)	123, 317, 691, 1133, 2233
Bsh1285I	CGRYCG	1 cut(s)	1197
BshFI	GGCC	3 cut(s)	779, 1760, 2104
BshNI	GGYRCC	2 cut(s)	859, 1067
BshVI	ATCGAT	1 cut(s)	2010
BsiEI	CGRYCG	1 cut(s)	1197
BsiHKAI	GWGCWC	2 cut(s)	700, 1623
BsiHKCI	CYCGRG	2 cut(s)	232, 1116
BsiSI	CCGG	5 cut(s)	108, 611, 776, 1159, 1587
BslFI	GGGAC	1 cut(s)	1356
BslI	CCNNNNNNNGG	6 cut(s)	744, 809, 869, 1499, 2100, 2225
BsmAI	GTCTC	2 cut(s)	1316, 1462
BsmFI	GGGAC	1 cut(s)	1356
BsmI	GAATGC	1 cut(s)	1738
BsnI	GGCC	3 cut(s)	779, 1760, 2104
BsoBI	CYCGRG	2 cut(s)	232, 1116
Bsp119I	TTCGAA	3 cut(s)	377, 928, 1443
Bsp1286I	GDGCHC	5 cut(s)	700, 862, 945, 1111, 1623
Bsp19I	CCATGG	1 cut(s)	808
BspACI	CCGC	7 cut(s)	72, 572, 1016, 1319, 1801, 1911, 2219
BspANI	GGCC	3 cut(s)	779, 1760, 2104
BspCNI	CTCAG	7 cut(s)	328, 485, 520, 1138, 1504, 2192, 2263
BspDI	ATCGAT	1 cut(s)	2010
BspHI	TCATGA	1 cut(s)	217
BspLI	GGNNCC	3 cut(s)	861, 1069, 1372
BspMI	ACCTGC	1 cut(s)	871
BspOI	GCTAGC	1 cut(s)	1707
BspPI	GGATC	6 cut(s)	22, 710, 901, 997, 1574, 2006
BspT104I	TTCGAA	3 cut(s)	377, 928, 1443
BspT107I	GGYRCC	2 cut(s)	859, 1067
BsrDI	GCAATG	2 cut(s)	761, 1096
BsrFI	RCCGGY	1 cut(s)	107
BsrI	ACTGG	5 cut(s)	104, 504, 792, 1308, 2004
BssAI	RCCGGY	1 cut(s)	107
BssECI	CCNNGG	2 cut(s)	808, 1157
BssNAI	GTATAC	1 cut(s)	1297
BssNI	GRCGYC	1 cut(s)	1872
BssSI	CACGAG	1 cut(s)	1538
BssT1I	CCWWGG	1 cut(s)	808
Bst1107I	GTATAC	1 cut(s)	1297
Bst2BI	CACGAG	1 cut(s)	1538
Bst2UI	CCWGG	2 cut(s)	2084, 2226
Bst4CI	ACNGT	4 cut(s)	826, 1274, 1593, 1691
Bst6I	CTCTTC	3 cut(s)	38, 620, 1913
BstACI	GRCGYC	1 cut(s)	1872
BstBAI	YACGTR	1 cut(s)	1825
BstBI	TTCGAA	3 cut(s)	377, 928, 1443
BstC8I	GCNNGC	3 cut(s)	781, 1705, 2060
BstDEI	CTNAG	9 cut(s)	315, 472, 528, 1032, 1125, 1512, 1737, 2179, 2250
BstDSI	CCRYGG	1 cut(s)	808
BstF5I	GGATG	4 cut(s)	204, 301, 1230, 1783
BstHHI	GCGC	2 cut(s)	258, 646
BstMAI	GTCTC	2 cut(s)	1316, 1462
BstMCI	CGRYCG	1 cut(s)	1197
BstMWI	GCNNNNNNNGC	5 cut(s)	704, 1028, 1037, 1818, 2110
BstNI	CCWGG	2 cut(s)	2084, 2226
BstNSI	RCATGY	3 cut(s)	650, 1481, 1549
BstSCI	CCNGG	3 cut(s)	1157, 2082, 2224
BstSFI	CTRYAG	2 cut(s)	934, 1876
BstSLI	GKGCMC	3 cut(s)	862, 1111, 1623
BstSNI	TACGTA	1 cut(s)	1825
BstV1I	GCAGC	5 cut(s)	123, 317, 691, 1133, 2233
BstX2I	RGATCY	1 cut(s)	2253
BstXI	CCANNNNNNTGG	2 cut(s)	412, 787
BstYI	RGATCY	1 cut(s)	2253
BstZ17I	GTATAC	1 cut(s)	1297
Bsu15I	ATCGAT	1 cut(s)	2010
BsuRI	GGCC	3 cut(s)	779, 1760, 2104
BsuTUI	ATCGAT	1 cut(s)	2010
BtgI	CCRYGG	1 cut(s)	808
BtsCI	GGATG	4 cut(s)	204, 301, 1230, 1783
BtsI	GCAGTG	2 cut(s)	1404, 2069
BtsIMutI	CAGTG	5 cut(s)	1279, 1404, 1509, 2069, 2187
BveI	ACCTGC	1 cut(s)	871
Cac8I	GCNNGC	3 cut(s)	781, 1705, 2060
CciI	TCATGA	1 cut(s)	217
CfoI	GCGC	2 cut(s)	258, 646
Cfr10I	RCCGGY	1 cut(s)	107
Cfr13I	GGNCC	2 cut(s)	1370, 1937
ClaI	ATCGAT	1 cut(s)	2010
Csp6I	GTAC	9 cut(s)	103, 922, 1068, 1257, 1543, 1826, 1867, 1923, 2214
CviQI	GTAC	9 cut(s)	103, 922, 1068, 1257, 1543, 1826, 1867, 1923, 2214
DdeI	CTNAG	9 cut(s)	315, 472, 528, 1032, 1125, 1512, 1737, 2179, 2250
DraI	TTTAAA	1 cut(s)	678
EaeI	YGGCCR	2 cut(s)	777, 1758
Eam1104I	CTCTTC	3 cut(s)	38, 620, 1913
EarI	CTCTTC	3 cut(s)	38, 620, 1913
EciI	GGCGGA	1 cut(s)	1816
Eco105I	TACGTA	1 cut(s)	1825
Eco130I	CCWWGG	1 cut(s)	808
Eco147I	AGGCCT	1 cut(s)	2104
Eco24I	GRGCYC	1 cut(s)	945
Eco47I	GGWCC	2 cut(s)	1370, 1937
Eco57I	CTGAAG	1 cut(s)	1418
Eco88I	CYCGRG	2 cut(s)	232, 1116
EcoRI	GAATTC	1 cut(s)	1439
EcoRII	CCWGG	2 cut(s)	2082, 2224
EcoT14I	CCWWGG	1 cut(s)	808
EcoT22I	ATGCAT	1 cut(s)	1972
EcoT38I	GRGCYC	1 cut(s)	945
ErhI	CCWWGG	1 cut(s)	808
FaqI	GGGAC	1 cut(s)	1356
FauI	CCCGC	1 cut(s)	2226
FauNDI	CATATG	5 cut(s)	290, 1042, 1816, 1972, 2210
FbaI	TGATCA	2 cut(s)	480, 1141
FblI	GTMKAC	4 cut(s)	1250, 1296, 1421, 1875
Fnu4HI	GCNGC	6 cut(s)	112, 306, 705, 1122, 1320, 2222
FokI	GGATG	4 cut(s)	191, 308, 1237, 1790
FriOI	GRGCYC	1 cut(s)	945
Fsp4HI	GCNGC	6 cut(s)	112, 306, 705, 1122, 1320, 2222
FspBI	CTAG	4 cut(s)	1235, 1254, 1487, 1704
GlaI	GCGC	2 cut(s)	257, 645
GluI	GCNGC	6 cut(s)	112, 306, 705, 1122, 1320, 2222
HaeIII	GGCC	3 cut(s)	779, 1760, 2104
HapII	CCGG	5 cut(s)	108, 611, 776, 1159, 1587
HhaI	GCGC	2 cut(s)	258, 646
Hin1I	GRCGYC	1 cut(s)	1872
Hin6I	GCGC	2 cut(s)	256, 644
HinP1I	GCGC	2 cut(s)	256, 644
HincII	GTYRAC	2 cut(s)	733, 1270
HindII	GTYRAC	2 cut(s)	733, 1270
HindIII	AAGCTT	1 cut(s)	1558
HinfI	GANTC	9 cut(s)	85, 398, 596, 617, 852, 1285, 1483, 1534, 2027
HpaI	GTTAAC	1 cut(s)	733
HpaII	CCGG	5 cut(s)	108, 611, 776, 1159, 1587
HphI	GGTGA	2 cut(s)	232, 1679
Hpy99I	CGWCG	2 cut(s)	1874, 2099
HpyAV	CCTTC	6 cut(s)	219, 384, 729, 1127, 1625, 1781
HpyCH4III	ACNGT	4 cut(s)	826, 1274, 1593, 1691
HpyCH4IV	ACGT	5 cut(s)	421, 1418, 1824, 1872, 2199
HpyF10VI	GCNNNNNNNGC	5 cut(s)	704, 1028, 1037, 1818, 2110
HpyF3I	CTNAG	9 cut(s)	315, 472, 528, 1032, 1125, 1512, 1737, 2179, 2250
HpySE526I	ACGT	5 cut(s)	421, 1418, 1824, 1872, 2199
Hsp92I	GRCGYC	1 cut(s)	1872
HspAI	GCGC	2 cut(s)	256, 644
KpnI	GGTACC	1 cut(s)	1071
Ksp22I	TGATCA	2 cut(s)	480, 1141
KspAI	GTTAAC	1 cut(s)	733
LmnI	GCTCC	2 cut(s)	285, 1285
Lsp1109I	GCAGC	5 cut(s)	123, 317, 691, 1133, 2233
LweI	GCATC	5 cut(s)	875, 1003, 1267, 2045, 2236
MaeI	CTAG	4 cut(s)	1235, 1254, 1487, 1704
MaeII	ACGT	5 cut(s)	421, 1418, 1824, 1872, 2199
MaeIII	GTNAC	5 cut(s)	95, 880, 1161, 1186, 2016
MfeI	CAATTG	1 cut(s)	1338
MflI	RGATCY	1 cut(s)	2253
MhlI	GDGCHC	5 cut(s)	700, 862, 945, 1111, 1623
MlsI	TGGCCA	1 cut(s)	1760
MluNI	TGGCCA	1 cut(s)	1760
MlyI	GAGTC	1 cut(s)	407
MmeI	TCCRAC	1 cut(s)	2180
Mox20I	TGGCCA	1 cut(s)	1760
Mph1103I	ATGCAT	1 cut(s)	1972
MroXI	GAANNNNTTC	1 cut(s)	2233
MscI	TGGCCA	1 cut(s)	1760
MseI	TTAA	8 cut(s)	462, 557, 677, 732, 761, 1002, 1455, 2241
MslI	CAYNNNNRTG	3 cut(s)	410, 813, 1932
Msp20I	TGGCCA	1 cut(s)	1760
MspA1I	CMGCKG	1 cut(s)	1265
MspI	CCGG	5 cut(s)	108, 611, 776, 1159, 1587
MspR9I	CCNGG	3 cut(s)	1159, 2084, 2226
MunI	CAATTG	1 cut(s)	1338
Mva1269I	GAATGC	1 cut(s)	1738
MvaI	CCWGG	2 cut(s)	2084, 2226
MwoI	GCNNNNNNNGC	5 cut(s)	704, 1028, 1037, 1818, 2110
NciI	CCSGG	1 cut(s)	1159
NcoI	CCATGG	1 cut(s)	808
NdeI	CATATG	5 cut(s)	290, 1042, 1816, 1972, 2210
NheI	GCTAGC	1 cut(s)	1703
NlaIV	GGNNCC	3 cut(s)	861, 1069, 1372
NmuCI	GTSAC	2 cut(s)	880, 2016
NsiI	ATGCAT	1 cut(s)	1972
NspI	RCATGY	3 cut(s)	650, 1481, 1549
NspV	TTCGAA	3 cut(s)	377, 928, 1443
PaeR7I	CTCGAG	1 cut(s)	232
PagI	TCATGA	1 cut(s)	217
PaqCI	CACCTGC	1 cut(s)	871
PceI	AGGCCT	1 cut(s)	2104
PciI	ACATGT	1 cut(s)	1477
PctI	GAATGC	1 cut(s)	1738
PdmI	GAANNNNTTC	1 cut(s)	2233
PfeI	GAWTC	8 cut(s)	85, 596, 617, 852, 1285, 1483, 1534, 2027
PfoI	TCCNGGA	1 cut(s)	2082
PkrI	GCNGC	6 cut(s)	113, 307, 706, 1123, 1321, 2223
PleI	GAGTC	1 cut(s)	406
PpsI	GAGTC	1 cut(s)	406
Ppu21I	YACGTR	1 cut(s)	1825
PscI	ACATGT	1 cut(s)	1477
PshBI	ATTAAT	1 cut(s)	1002
Psp1406I	AACGTT	1 cut(s)	421
Psp6I	CCWGG	2 cut(s)	2082, 2224
PspGI	CCWGG	2 cut(s)	2082, 2224
PspN4I	GGNNCC	3 cut(s)	861, 1069, 1372
PspPI	GGNCC	2 cut(s)	1370, 1937
PsuI	RGATCY	1 cut(s)	2253
PvuII	CAGCTG	1 cut(s)	1265
RsaI	GTAC	9 cut(s)	104, 923, 1069, 1258, 1544, 1827, 1868, 1924, 2215
RsaNI	GTAC	9 cut(s)	103, 922, 1068, 1257, 1543, 1826, 1867, 1923, 2214
RseI	CAYNNNNRTG	3 cut(s)	410, 813, 1932
SaqAI	TTAA	8 cut(s)	462, 557, 677, 732, 761, 1002, 1455, 2241
SatI	GCNGC	6 cut(s)	112, 306, 705, 1122, 1320, 2222
Sau96I	GGNCC	2 cut(s)	1370, 1937
SchI	GAGTC	1 cut(s)	407
ScrFI	CCNGG	3 cut(s)	1159, 2084, 2226
SduI	GDGCHC	5 cut(s)	700, 862, 945, 1111, 1623
SfaNI	GCATC	5 cut(s)	875, 1003, 1267, 2045, 2236
SfcI	CTRYAG	2 cut(s)	934, 1876
Sfr274I	CTCGAG	1 cut(s)	232
SfuI	TTCGAA	3 cut(s)	377, 928, 1443
SinI	GGWCC	2 cut(s)	1370, 1937
SlaI	CTCGAG	1 cut(s)	232
SmiMI	CAYNNNNRTG	3 cut(s)	410, 813, 1932
SmlI	CTYRAG	3 cut(s)	232, 1425, 2145
SmoI	CTYRAG	3 cut(s)	232, 1425, 2145
SnaBI	TACGTA	1 cut(s)	1825
SpeI	ACTAGT	1 cut(s)	1253
SseBI	AGGCCT	1 cut(s)	2104
SsiI	CCGC	7 cut(s)	72, 572, 1016, 1319, 1801, 1911, 2219
SspMI	CTAG	4 cut(s)	1235, 1254, 1487, 1704
StuI	AGGCCT	1 cut(s)	2104
StyD4I	CCNGG	3 cut(s)	1157, 2082, 2224
StyI	CCWWGG	1 cut(s)	808
TaaI	ACNGT	4 cut(s)	826, 1274, 1593, 1691
TaiI	ACGT	5 cut(s)	424, 1421, 1827, 1875, 2202
TaqII	GACCGA	1 cut(s)	1183
TatI	WGTACW	3 cut(s)	1256, 1542, 1922
TauI	GCSGC	1 cut(s)	1322
TfiI	GAWTC	8 cut(s)	85, 596, 617, 852, 1285, 1483, 1534, 2027
Tru1I	TTAA	8 cut(s)	462, 557, 677, 732, 761, 1002, 1455, 2241
Tru9I	TTAA	8 cut(s)	462, 557, 677, 732, 761, 1002, 1455, 2241
TscAI	CASTG	5 cut(s)	1279, 1404, 1516, 2076, 2187
TseFI	GTSAC	2 cut(s)	880, 2016
TseI	GCWGC	5 cut(s)	111, 305, 704, 1121, 2221
Tsp45I	GTSAC	2 cut(s)	880, 2016
TspGWI	ACGGA	2 cut(s)	1930, 2004
TspRI	CASTG	5 cut(s)	1279, 1404, 1516, 2076, 2187
VneI	GTGCAC	1 cut(s)	1619
VpaK11BI	GGWCC	2 cut(s)	1370, 1937
VspI	ATTAAT	1 cut(s)	1002
XapI	RAATTY	3 cut(s)	181, 631, 1439
XceI	RCATGY	3 cut(s)	650, 1481, 1549
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1558
XhoI	CTCGAG	1 cut(s)	232
XmiI	GTMKAC	4 cut(s)	1250, 1296, 1421, 1875
XmnI	GAANNNNTTC	1 cut(s)	2233
XspI	CTAG	4 cut(s)	1235, 1254, 1487, 1704
ZraI	GACGTC	1 cut(s)	1873
Zsp2I	ATGCAT	1 cut(s)	1972

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.