Prupe.7G258600_v2.0.a1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0000775
Physical Location & Seq
Forward (+)
21575579 .. 21579696
4118 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G258600.1

Sequence Viewer

Length: 2325 bp
ATGGTATTCAAATTTTCAAGTGTACTACTAGTCCTCCTTTTCTTCCATTTTCTTGTTGCTTTTAGACTGCAAAGCTCAGAGGCACAATCTTGGATCAAAGCAGCATACTGGTATTCTGGCAGTGAATTCCCCATTGCTGACATAAACTCTGCGCTTTTTACTCACCTTATTTACTCATTTGCCGGTCTTAATTCATCATCCTATGAGCTCTCCCTCTCCTCTGACTCTGAGGAGCAGGATCAGGTGCAGCACTACTCCACCTTCTCATCCACGGCCAAGCAAAAGAACCCATCAGTCACCACCCTCATCTCCATTGGGGGTGGAAACGCAGACTACTCAGTCTTTTCAACACTTGTCAGTAATTCTTCTCACAGAAAGACCTTCATTGATTCTTCAATAAAGATTGCAAGGCTTTATGGCTTCCAAGGCCTAGACCTTTGCTGGGTTTCAGCTAACACAAGCGCGGACATGACCAACATGGGAAATTTGTTTCAAGAGTGGCGAGCTGCTGTCAAATCTGAGGCTGCAAATTCTAGCCAGGCGCAGCTGATTTTGACAGCAGCCGTTCAATACAAACCAGATTTGGAGTCCTTTTCTTTTCCGGTTGAATCCATTCGGAACAACTTGAATTGGGTACATGTAATGGCCTATGACTATTATGGGCCTCAGTGGACTAATTTCACCGGCGCTCATGCAGCTCTGTATGATCCATCAAGTGATGCTAGCACAGATTATGGTATCAAGGCATGGATTGGTAAGGGATTATCTGCTTCCAAATTGGTTTTGGGCTTGCCTTTCTATGGCTATGCTTGGAAACTTGTGAATCCTAGGGACAATGGTATTGGTGCACCAGCAACAGGTCCAGCCATTACAGAGGATGGATCCATGAGCTACAAGGATATCAAGGGTTACATTCAGAGATATGGGGCTGATATAATCTACAATGCTACGTATGTAGTTAAGTACTGCATAATTGGATCATCTTGGATTGGTTTTGATGATGCTCAAGTTGTCAAAATAAAAGTTTCTTATGCTAAGGAGCAGAGTTTGCTTGGATACTTTGTCTGGCAGGTTTCCCATGATGACAACTGGGTGCTTTCTCTTGCAGCTGCAGCTACAGCTCAAGAGCGTGCAAGCAGTGGACAAAAAAAGCGGCGACTTCTTATAATCATTTTGACTACAACAGCTTCAGTTGTTCTCATATTAGGCTCCGTCATGTGTTACTTCCGCATGAGAATGTTCAAGTCAAAAGCTAAAGAATCAGAATCCAGGGCAAATTATATAGCAGACCCAGCTGGAACTTTTAATAGCAATGTACCTAATCTGCAAGTCTTTGGCATTGCTGTAATTGAGGAGGCAACAGAAGGATTTTCAATTGAAAATAAGCTTGGAGAGGGTGGTTATGGTCCAGTTTATAAGGGTGTATTGCCAAATGGACAAGAAATAGCTGTGAAAAAACTTTCAAAAGCTTCAACTCAAGGATTCGAGGAATTCAAGAACGAGGTCATGCTTACAGCAAAACTACAACACGTTAATCTTGTCAGGGTTTTGGGATTCTGCATCGAACACCATGAACAAATGCTGATTTATGAGTACATGCCAAAGAAAAGCTTGGATCTCTACCTCTTTGATCCTATCAGAAGGTATCAACTAGATTGGAGAAAACGTGTTAGCATTATTGAAGGGGTTACTCAGGGACTTCTTTATCTCCAAGAATACTCAAGATTGACAATAATCCATCGAGATCTGAAAGCTAGCAACATTTTACTTGATGAGGAGATGAGGCCTAAGATCTCAGATTTTGGCATGGCTAGAATTTTCACAAAAGATGAACAAGAGGCAAACACAAGCCGGATCGTTGGAACATATGGCTATGTACCTCCTGAATATGTTAAAAAAGGTCTATACTCCACGAAATCAGATGTTTATAGCTTTGGAGTTCTCCTTGTACAGATCATAAGTGGCAAGAAGAATGCCGCTTTCTATGGTCCAAATGAAGACTTAAACCTTCTAGAATACGCATATGAACTTTGGAAAGAAGGCAAAGGGATGGAGTTTATGGATCCTTCACTTGATGATACACATTCATTATGTAAATTGATGAGATGCTTGCAGATTGCTCTCCTATGTGTTCAAGAAAAGGCAAATGATAGGCCTTCCATGTTGGAAATTTCTTCGATGCTACAGAGTGAAAATGCTGTAATGGAAAAACCGAAAATGCCAGCTTTTTCAAAAAGAAATGAAGACAAAGAAACTAATCCTGCACCGCGGCTAGAAGTGTGTTCCATTGATGATTCAACAATCTCAGAGGTTGTAGGCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18 Pkinase Pkinase_Tyr

Protein Analysis

775

Amino Acids

86.68

Weight (kDa)

6.14

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1166, 1416
AasI GACNNNNNNGTC 1 cut(s) 338
Acc36I ACCTGC 1 cut(s) 1060
AccII CGCG 2 cut(s) 464, 2269
AciI CCGC 6 cut(s) 464, 1153, 1228, 1977, 2267, 2269
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 7 cut(s) 11, 125, 484, 529, 1490, 1815, 2169
AcuI CTGAAG 1 cut(s) 1173
AfaI GTAC 7 cut(s) 24, 636, 965, 1317, 1595, 1878, 1950
AfiI CCNNNNNNNGG 3 cut(s) 442, 800, 857
AflIII ACRYGT 3 cut(s) 637, 1528, 1666
AhlI ACTAGT 1 cut(s) 28
AjnI CCWGG 2 cut(s) 537, 1268
AjuI GAANNNNNNNTTGG 2 cut(s) 1371, 1403
Alw21I GWGCWC 2 cut(s) 210, 850
Alw44I GTGCAC 1 cut(s) 846
AoxI GGCC 7 cut(s) 273, 427, 645, 662, 1784, 2153, 2317
ApaLI GTGCAC 1 cut(s) 846
ApoI RAATTY 7 cut(s) 11, 125, 484, 529, 1490, 1815, 2169
Asp700I GAANNNNTTC 1 cut(s) 612
AspA2I CCTAGG 1 cut(s) 827
AspLEI GCGC 4 cut(s) 154, 464, 544, 689
AspS9I GGNCC 4 cut(s) 662, 860, 1406, 1988
AsuHPI GGTGA 3 cut(s) 155, 289, 673
AsuNHI GCTAGC 2 cut(s) 722, 1754
AvaII GGWCC 3 cut(s) 860, 1406, 1988
AvrII CCTAGG 1 cut(s) 827
BaeGI GKGCMC 1 cut(s) 850
BamHI GGATCC 2 cut(s) 881, 2062
BanII GRGCYC 1 cut(s) 210
BbsI GAAGAC 2 cut(s) 2004, 2250
Bbv12I GWGCWC 2 cut(s) 210, 850
BccI CCATC 5 cut(s) 298, 718, 872, 1746, 2044
BceAI ACGGC 2 cut(s) 288, 548
BciT130I CCWGG 2 cut(s) 539, 1270
BciVI GTATCC 1 cut(s) 1049
BcuI ACTAGT 1 cut(s) 28
BfmI CTRYAG 3 cut(s) 1110, 1116, 2183
BfoI RGCGCY 1 cut(s) 690
BfuAI ACCTGC 1 cut(s) 1060
BfuI GTATCC 1 cut(s) 1049
BglII AGATCT 2 cut(s) 1744, 1791
BlnI CCTAGG 1 cut(s) 827
BmcAI AGTACT 1 cut(s) 965
Bme1390I CCNGG 2 cut(s) 539, 1270
Bme18I GGWCC 3 cut(s) 860, 1406, 1988
BmgT120I GGNCC 4 cut(s) 662, 860, 1406, 1988
BmiI GGNNCC 3 cut(s) 883, 1210, 2064
BmrFI CCNGG 2 cut(s) 539, 1270
BmrI ACTGGG 1 cut(s) 1099
BmsI GCATC 5 cut(s) 709, 991, 1569, 2096, 2169
BmtI GCTAGC 2 cut(s) 726, 1758
BmuI ACTGGG 1 cut(s) 1099
BpiI GAAGAC 2 cut(s) 2004, 2250
Bpu10I CCTNAGC 1 cut(s) 1035
BpuEI CTTGAG 4 cut(s) 990, 1107, 1461, 1705
BsaAI YACGTR 1 cut(s) 951
BsaJI CCNNGG 5 cut(s) 270, 424, 827, 1269, 2267
BsaWI WCCGGW 1 cut(s) 601
BsaXI ACNNNNNCTCC 4 cut(s) 18, 48, 1651, 1681
Bsc4I CCNNNNNNNGG 3 cut(s) 442, 800, 857
Bse118I RCCGGY 2 cut(s) 182, 683
Bse1I ACTGG 3 cut(s) 113, 1094, 1409
Bse3DI GCAATG 3 cut(s) 132, 1318, 1338
BseBI CCWGG 2 cut(s) 539, 1270
BseDI CCNNGG 5 cut(s) 270, 424, 827, 1269, 2267
BseGI GGATG 4 cut(s) 197, 266, 883, 2055
BseLI CCNNNNNNNGG 3 cut(s) 442, 800, 857
BseMI GCAATG 3 cut(s) 132, 1318, 1338
BseMII CTCAG 8 cut(s) 90, 219, 351, 510, 680, 1706, 1809, 2319
BseNI ACTGG 3 cut(s) 113, 1094, 1409
BseRI GAGGAG 4 cut(s) 208, 245, 1367, 1790
BseSI GKGCMC 1 cut(s) 850
BseYI CCCAGC 2 cut(s) 441, 1291
BsgI GTGCAG 2 cut(s) 266, 2247
Bsh1236I CGCG 2 cut(s) 464, 2269
BshFI GGCC 7 cut(s) 275, 429, 647, 664, 1786, 2155, 2319
BsiHKAI GWGCWC 2 cut(s) 210, 850
BsiSI CCGG 4 cut(s) 183, 602, 684, 1852
BslFI GGGAC 2 cut(s) 845, 1710
BslI CCNNNNNNNGG 3 cut(s) 442, 800, 857
BsmFI GGGAC 2 cut(s) 845, 1710
BsmI GAATGC 1 cut(s) 1978
BsnI GGCC 7 cut(s) 275, 429, 647, 664, 1786, 2155, 2319
Bsp1286I GDGCHC 2 cut(s) 210, 850
Bsp1407I TGTACA 1 cut(s) 1948
BspACI CCGC 6 cut(s) 464, 1153, 1228, 1977, 2267, 2269
BspANI GGCC 7 cut(s) 275, 429, 647, 664, 1786, 2155, 2319
BspCNI CTCAG 8 cut(s) 89, 220, 350, 511, 679, 1705, 1808, 2318
BspFNI CGCG 2 cut(s) 464, 2269
BspLI GGNNCC 3 cut(s) 883, 1210, 2064
BspMAI CTGCAG 1 cut(s) 1114
BspMI ACCTGC 1 cut(s) 1060
BspOI GCTAGC 2 cut(s) 726, 1758
BsrDI GCAATG 3 cut(s) 132, 1318, 1338
BsrFI RCCGGY 2 cut(s) 182, 683
BsrGI TGTACA 1 cut(s) 1948
BsrI ACTGG 3 cut(s) 113, 1094, 1409
BssAI RCCGGY 2 cut(s) 182, 683
BssECI CCNNGG 5 cut(s) 270, 424, 827, 1269, 2267
BssT1I CCWWGG 2 cut(s) 424, 827
Bst2UI CCWGG 2 cut(s) 539, 1270
BstAPI GCANNNNNTGC 1 cut(s) 1048
BstAUI TGTACA 1 cut(s) 1948
BstBAI YACGTR 1 cut(s) 951
BstC8I GCNNGC 8 cut(s) 504, 724, 791, 1131, 1135, 1756, 2111, 2223
BstDSI CCRYGG 2 cut(s) 270, 2267
BstF5I GGATG 4 cut(s) 197, 266, 883, 2055
BstFNI CGCG 2 cut(s) 464, 2269
BstH2I RGCGCY 1 cut(s) 690
BstHHI GCGC 4 cut(s) 154, 464, 544, 689
BstMWI GCNNNNNNNGC 6 cut(s) 426, 695, 1048, 1112, 1118, 1292
BstNI CCWGG 2 cut(s) 539, 1270
BstNSI RCATGY 2 cut(s) 641, 1600
BstSCI CCNGG 2 cut(s) 537, 1268
BstSFI CTRYAG 3 cut(s) 1110, 1116, 2183
BstSLI GKGCMC 1 cut(s) 850
BstSNI TACGTA 1 cut(s) 951
BstUI CGCG 2 cut(s) 464, 2269
BstV2I GAAGAC 2 cut(s) 2004, 2250
BstX2I RGATCY 5 cut(s) 881, 1615, 1744, 1791, 2062
BstYI RGATCY 5 cut(s) 881, 1615, 1744, 1791, 2062
BsuI GTATCC 1 cut(s) 1049
BsuRI GGCC 7 cut(s) 275, 429, 647, 664, 1786, 2155, 2319
BtgI CCRYGG 2 cut(s) 270, 2267
BtsCI GGATG 4 cut(s) 197, 266, 883, 2055
BtsI GCAGTG 2 cut(s) 127, 1144
BtsIMutI CAGTG 3 cut(s) 127, 674, 1144
BveI ACCTGC 1 cut(s) 1060
Cac8I GCNNGC 8 cut(s) 504, 724, 791, 1131, 1135, 1756, 2111, 2223
CfoI GCGC 4 cut(s) 154, 464, 544, 689
Cfr10I RCCGGY 2 cut(s) 182, 683
Cfr13I GGNCC 4 cut(s) 662, 860, 1406, 1988
Cfr42I CCGCGG 1 cut(s) 2270
Csp6I GTAC 7 cut(s) 23, 635, 964, 1316, 1594, 1877, 1949
CviQI GTAC 7 cut(s) 23, 635, 964, 1316, 1594, 1877, 1949
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 273
Ecl136II GAGCTC 1 cut(s) 208
Eco105I TACGTA 1 cut(s) 951
Eco130I CCWWGG 2 cut(s) 424, 827
Eco147I AGGCCT 3 cut(s) 429, 1786, 2155
Eco24I GRGCYC 1 cut(s) 210
Eco32I GATATC 1 cut(s) 901
Eco47I GGWCC 3 cut(s) 860, 1406, 1988
Eco53kI GAGCTC 1 cut(s) 208
Eco57I CTGAAG 1 cut(s) 1173
EcoICRI GAGCTC 1 cut(s) 208
EcoRI GAATTC 2 cut(s) 125, 1490
EcoRII CCWGG 2 cut(s) 537, 1268
EcoRV GATATC 1 cut(s) 901
EcoT14I CCWWGG 2 cut(s) 424, 827
EcoT38I GRGCYC 1 cut(s) 210
ErhI CCWWGG 2 cut(s) 424, 827
FalI AAGNNNNNCTT 2 cut(s) 1595, 1627
FaqI GGGAC 2 cut(s) 845, 1710
FauNDI CATATG 2 cut(s) 1867, 2023
FokI GGATG 4 cut(s) 184, 253, 890, 2062
FriOI GRGCYC 1 cut(s) 210
GlaI GCGC 4 cut(s) 153, 463, 543, 688
GsaI CCCAGC 2 cut(s) 445, 1295
HaeII RGCGCY 1 cut(s) 690
HaeIII GGCC 7 cut(s) 275, 429, 647, 664, 1786, 2155, 2319
HapII CCGG 4 cut(s) 183, 602, 684, 1852
HhaI GCGC 4 cut(s) 154, 464, 544, 689
Hin6I GCGC 4 cut(s) 152, 462, 542, 687
HinP1I GCGC 4 cut(s) 152, 462, 542, 687
HindIII AAGCTT 3 cut(s) 1385, 1467, 1609
HpaII CCGG 4 cut(s) 183, 602, 684, 1852
HphI GGTGA 3 cut(s) 155, 289, 673
Hpy166II GTNNAC 4 cut(s) 23, 672, 848, 1142
Hpy188III TCNNGA 8 cut(s) 494, 1124, 1495, 1722, 1742, 1883, 2012, 2135
Hpy8I GTNNAC 4 cut(s) 23, 672, 848, 1142
HpyAV CCTTC 9 cut(s) 271, 391, 1358, 1635, 1676, 2018, 2033, 2076, 2166
HpyCH4IV ACGT 3 cut(s) 950, 1530, 1666
HpyF10VI GCNNNNNNNGC 6 cut(s) 426, 695, 1048, 1112, 1118, 1292
HpySE526I ACGT 3 cut(s) 950, 1530, 1666
HspAI GCGC 4 cut(s) 152, 462, 542, 687
KspI CCGCGG 1 cut(s) 2270
LmnI GCTCC 3 cut(s) 232, 1039, 1214
LweI GCATC 5 cut(s) 709, 991, 1569, 2096, 2169
MaeII ACGT 3 cut(s) 950, 1530, 1666
MaeIII GTNAC 4 cut(s) 295, 908, 1220, 1687
MboII GAAGA 7 cut(s) 34, 357, 384, 1981, 2009, 2166, 2255
MfeI CAATTG 1 cut(s) 1374
MflI RGATCY 5 cut(s) 881, 1615, 1744, 1791, 2062
MhlI GDGCHC 2 cut(s) 210, 850
MlyI GAGTC 2 cut(s) 218, 596
MmeI TCCRAC 2 cut(s) 1840, 2145
MroXI GAANNNNTTC 1 cut(s) 612
MseI TTAA 6 cut(s) 189, 960, 1305, 1533, 1893, 2003
MslI CAYNNNNRTG 1 cut(s) 1235
MspA1I CMGCKG 4 cut(s) 547, 1109, 1295, 2269
MspI CCGG 4 cut(s) 183, 602, 684, 1852
MspR9I CCNGG 2 cut(s) 539, 1270
MunI CAATTG 1 cut(s) 1374
Mva1269I GAATGC 1 cut(s) 1978
MvaI CCWGG 2 cut(s) 539, 1270
MvnI CGCG 2 cut(s) 464, 2269
MwoI GCNNNNNNNGC 6 cut(s) 426, 695, 1048, 1112, 1118, 1292
NdeI CATATG 2 cut(s) 1867, 2023
NheI GCTAGC 2 cut(s) 722, 1754
NlaIV GGNNCC 3 cut(s) 883, 1210, 2064
NmuCI GTSAC 1 cut(s) 295
NspI RCATGY 2 cut(s) 641, 1600
PceI AGGCCT 3 cut(s) 429, 1786, 2155
PciI ACATGT 1 cut(s) 637
PctI GAATGC 1 cut(s) 1978
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 8 cut(s) 389, 608, 823, 1259, 1265, 1482, 1554, 2294
PleI GAGTC 2 cut(s) 218, 595
PpsI GAGTC 2 cut(s) 218, 595
Ppu21I YACGTR 1 cut(s) 951
PscI ACATGT 1 cut(s) 637
PsiI TTATAA 2 cut(s) 1166, 1416
Psp124BI GAGCTC 1 cut(s) 210
Psp6I CCWGG 2 cut(s) 537, 1268
PspFI CCCAGC 2 cut(s) 441, 1291
PspGI CCWGG 2 cut(s) 537, 1268
PspN4I GGNNCC 3 cut(s) 883, 1210, 2064
PspPI GGNCC 4 cut(s) 662, 860, 1406, 1988
PstI CTGCAG 1 cut(s) 1114
PsuI RGATCY 5 cut(s) 881, 1615, 1744, 1791, 2062
PvuII CAGCTG 3 cut(s) 547, 1109, 1295
RsaI GTAC 7 cut(s) 24, 636, 965, 1317, 1595, 1878, 1950
RsaNI GTAC 7 cut(s) 23, 635, 964, 1316, 1594, 1877, 1949
RseI CAYNNNNRTG 1 cut(s) 1235
SacI GAGCTC 1 cut(s) 210
SacII CCGCGG 1 cut(s) 2270
SaqAI TTAA 6 cut(s) 189, 960, 1305, 1533, 1893, 2003
Sau96I GGNCC 4 cut(s) 662, 860, 1406, 1988
ScaI AGTACT 1 cut(s) 965
SchI GAGTC 2 cut(s) 218, 596
ScrFI CCNGG 2 cut(s) 539, 1270
SduI GDGCHC 2 cut(s) 210, 850
SfaNI GCATC 5 cut(s) 709, 991, 1569, 2096, 2169
SfcI CTRYAG 3 cut(s) 1110, 1116, 2183
Sfr303I CCGCGG 1 cut(s) 2270
SgrAI CRCCGGYG 1 cut(s) 683
SgrBI CCGCGG 1 cut(s) 2270
SinI GGWCC 3 cut(s) 860, 1406, 1988
SmiMI CAYNNNNRTG 1 cut(s) 1235
SmlI CTYRAG 4 cut(s) 1005, 1122, 1476, 1720
SmoI CTYRAG 4 cut(s) 1005, 1122, 1476, 1720
SnaBI TACGTA 1 cut(s) 951
SpeI ACTAGT 1 cut(s) 28
SseBI AGGCCT 3 cut(s) 429, 1786, 2155
SsiI CCGC 6 cut(s) 464, 1153, 1228, 1977, 2267, 2269
SstI GAGCTC 1 cut(s) 210
StuI AGGCCT 3 cut(s) 429, 1786, 2155
StyD4I CCNGG 2 cut(s) 537, 1268
StyI CCWWGG 2 cut(s) 424, 827
TaiI ACGT 3 cut(s) 953, 1533, 1669
TaqI TCGA 4 cut(s) 1485, 1563, 1741, 2177
TatI WGTACW 4 cut(s) 22, 963, 1593, 1948
TauI GCSGC 3 cut(s) 1156, 1979, 2272
TfiI GAWTC 8 cut(s) 389, 608, 823, 1259, 1265, 1482, 1554, 2294
Tru1I TTAA 6 cut(s) 189, 960, 1305, 1533, 1893, 2003
Tru9I TTAA 6 cut(s) 189, 960, 1305, 1533, 1893, 2003
TscAI CASTG 3 cut(s) 127, 674, 1144
TseFI GTSAC 1 cut(s) 295
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 8 cut(s) 183, 373, 1587, 1845, 2010, 2040, 2076, 2256
TspGWI ACGGA 1 cut(s) 1201
TspRI CASTG 3 cut(s) 127, 674, 1144
VneI GTGCAC 1 cut(s) 846
VpaK11BI GGWCC 3 cut(s) 860, 1406, 1988
XapI RAATTY 7 cut(s) 11, 125, 484, 529, 1490, 1815, 2169
XbaI TCTAGA 1 cut(s) 2011
XceI RCATGY 2 cut(s) 641, 1600
XcmI CCANNNNNNNNNTGG 1 cut(s) 781
XmaJI CCTAGG 1 cut(s) 827
XmnI GAANNNNTTC 1 cut(s) 612
ZrmI AGTACT 1 cut(s) 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.