MD15G1156900.v1.1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000775
Physical Location & Seq
Forward (+)
11651488 .. 11654801
3314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1156900.v1.1.491

Sequence Viewer

Length: 2310 bp
ATGGTATCCAAATTTTTAGTTCTACCATTCTTCTTCTTTTTCCATATTTTTTTTGCTTTTAGAATCCAACCTTCGGAGGCACAATCTTGGATCAAAGTTGCATACTGGTATTCCGGCAGCAAATTCCCTATTTCCGACATCAACTCTCCGCTCTTCACTCACCTTATTTACGCTTACAAAGATGTTAACTCATCATCCTATGAGCTCTCTTCCTCCTCCTCCGACTCCTCGGAGCAGTATTACTCCACCTTCACGCCAACCGTCAAGCGAAAGAACCCATCAGTCACTACTCTCGTCTCCATTGGCGGTGGAAACGCAGACTACGGTGTCATTTCAACCATGCTCAGTAGCTCTTCTCACAGAAATTCTTTCATTGATTCTTCGATAATTTTCGCAAGGCGTTATGGCTTCCTAGGCCTAGACTTTTGTTGGGTTTCAGCTAACACAAGCTCGGACATGACCAACATGGGGAAGTTATTTGAAGAGTGGAGGGCAGCTATCGCTTCTGAAGCCGCAAAAAACAGCCAAACACCACTGATTTTGACTGCTGCCGTGCACTACAAGCCAGATGTTGAGTCCTTCGCTTTCCCGGTTGAATCAATCCGGGACAACTTGAACTGGGTACATGTCTTGGCCTATGACTATTACGGACCTCGGTGGACCAATGTCACTGGTGCTCATGCAGCACTATACAATCCATCGAGTATCTGGAGTACGGACTATGGCATCAAATCATGGATTGGCAGGGGATTATCTGCTTCCAAACTGGTTTTGGGATTGCCTTTCTATGGATATGCTTGGACGCTAAGCAATCCTAGGGACAGCGCTATTGGCGCACCGGCTAAAGGTCCTGCTAATATGACGGAGGATGGATCGATGAGCTACAAGCATATCAAACGCTACATTGATAGACATGGGGCTGACATAGTCTACAATACTACTTATGTAGCGAAGTACTGTGTGATTGGTTCGGTTTGGATCTGTTTCGATGATGCTGAAGTTGTGAAAATAAAAGTTTCTTATGCTAAGGAGCAGAATTTGCTTGGATACTTCATCTGGCAGGTCTCACAGGATGATAATTGGGTGCTTTCTCTTGCAGCTGCAGCTCAAGAGGGTGGAAGCAGTGGACGAAACAAACGGAGACTTCTTTTAATCGTTTTGACCACTGTAGCTTCAGTTATTCTCGTGCTAGGCTCTGCGTTGAGTTACTTCCGCATGAGACTGCACAAATCAAAAGCTAAGGAATCAGAATCCAGGGCAAATGATATAGCAGACACAGCTGGAAAATTCAACAGCAACGTTCCTAATCTGAAAATATTTAGCATTGCTGACGTTGAGGCGGCAACGGATGGATTTTCGATTGAAAATAAGCTTGGAGAGGGCGGTTATGGCCCCGTTTATAAGGGAGTATTGCCAGATGGACAAGAAATCGCTGTGAAGAAACTATCAAAAGCTTCAACTCAAGGATTTGAGGAGTTCAAGAACGAGGTCATGCTCACCGCAAAACTACAACACGTAAATCTTGTTAGGGTTTTGGGATTTTGCATCGAACACCATGAGCAAATGCTGATATACGAATACATGCCAAAGAAAAGCTTGGACCTCTACCTCTTTGATCCTGTTAGAAGATATGAATTGGATTGGAGAAAGCGGGTTCAAATTATCGAAGGGGTTACTCAGGGACTTCTTTACCTCCAAGAGTACTCAAGATTGACAATCATCCATCGCGATTTGAAAGCTAGCAACATTTTACTTGATGAGGAGATGAGGCCTAAGATCTCGGATTTTGGTATGGCCAGAATTTTCGCAAAGGAGGAACTCGAAGCAAATACAGGTCGAATTGTTGGAACATATGGTTACGTACCACCGGAATACGTTAAGAAGGGTTTATACTCCACTAAATCAGATGTTTACAGCTTCGGAGTTCTCTTTCTACAGATCATAAGTGGCAAGAAGAATGCCCCCTACTATGGTTCAGATGAAGACTTAAACCTACTAGAATATGCATATTTACTCTGGAAACAAGGCAAAGGGATGGAGTTTATGGATCATGCGCTTGACGATACACATTCTTTATGCAAATTGATGAGATGCTTGCAAATTGCTCTCCTATGTGTTCAAGAAAATGCAAACGATAGGCCTTCGATGTTGGAAATTTCTTCAATGCTACAAAATGAAGGAGCTACAATGGAAAATCCCAAAATTCCAGCCTTCTCAAAAAGAAAAGAAGACGAAGAAACTAATCCCACGCCGCGGCTAGAAGCTTGTTCCATCAATGATGCAACAATCTCTGAAGTTGTAGCCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18 Pkinase Pkinase_Tyr

Protein Analysis

770

Amino Acids

86.48

Weight (kDa)

6.6

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 33 - 358 6.2e-66 Glycosyl hydrolases family 18
PK_Tyr_Ser-Thr PF07714 453 - 722 1.8e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 453 - 719 5.4e-43 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1401
AasI GACNNNNNNGTC 1 cut(s) 326
Acc36I ACCTGC 1 cut(s) 1051
AccBSI CCGCTC 1 cut(s) 151
AccI GTMKAC 1 cut(s) 930
AccII CGCG 2 cut(s) 1728, 2254
AclI AACGTT 1 cut(s) 1299
AclWI GGATC 5 cut(s) 98, 880, 986, 1610, 2055
AcoI YGGCCR 1 cut(s) 1794
AcsI RAATTY 8 cut(s) 11, 122, 364, 1036, 1286, 1800, 2154, 2202
AcuI CTGAAG 3 cut(s) 528, 1017, 1158
AfaI GTAC 5 cut(s) 624, 715, 956, 1703, 1863
AfeI AGCGCT 1 cut(s) 826
AfiI CCNNNNNNNGG 6 cut(s) 73, 468, 788, 845, 1970, 2253
AflIII ACRYGT 2 cut(s) 625, 1513
AjnI CCWGG 1 cut(s) 1253
AjuI GAANNNNNNNTTGG 2 cut(s) 1356, 1388
Alw21I GWGCWC 3 cut(s) 207, 558, 679
Alw26I GTCTC 4 cut(s) 301, 1069, 1135, 1213
Alw44I GTGCAC 1 cut(s) 554
AlwI GGATC 5 cut(s) 98, 880, 986, 1610, 2055
Aor51HI AGCGCT 1 cut(s) 826
AoxI GGCC 6 cut(s) 415, 633, 1390, 1769, 1794, 2138
ApaLI GTGCAC 1 cut(s) 554
ApeKI GCWGC 7 cut(s) 117, 494, 548, 683, 1097, 1100, 1103
ApoI RAATTY 8 cut(s) 11, 122, 364, 1036, 1286, 1800, 2154, 2202
AspA2I CCTAGG 2 cut(s) 412, 815
AspLEI GCGC 3 cut(s) 827, 836, 2056
AspS9I GGNCC 5 cut(s) 650, 660, 848, 1391, 1600
AsuC2I CCSGG 2 cut(s) 590, 605
AsuHPI GGTGA 2 cut(s) 152, 1489
AsuNHI GCTAGC 1 cut(s) 1739
AvaII GGWCC 4 cut(s) 650, 660, 848, 1600
AvrII CCTAGG 2 cut(s) 412, 815
BaeGI GKGCMC 1 cut(s) 558
BalI TGGCCA 1 cut(s) 1796
BanII GRGCYC 1 cut(s) 207
BarI GAAGNNNNNNTAC 2 cut(s) 1874, 1906
BauI CACGAG 1 cut(s) 1184
BbsI GAAGAC 2 cut(s) 1989, 2235
Bbv12I GWGCWC 3 cut(s) 207, 558, 679
BbvI GCAGC 7 cut(s) 129, 506, 535, 695, 1087, 1109, 1115
BccI CCATC 8 cut(s) 286, 706, 863, 1343, 1412, 1731, 2029, 2279
BceAI ACGGC 1 cut(s) 536
BciT130I CCWGG 1 cut(s) 1255
BciVI GTATCC 2 cut(s) 16, 1040
BcnI CCSGG 2 cut(s) 590, 605
BcoDI GTCTC 4 cut(s) 301, 1069, 1135, 1213
BfaI CTAG 7 cut(s) 413, 419, 816, 1190, 1740, 1997, 2258
BfmI CTRYAG 3 cut(s) 1101, 1167, 1934
BfoI RGCGCY 1 cut(s) 828
BfuAI ACCTGC 1 cut(s) 1051
BfuI GTATCC 2 cut(s) 16, 1040
BglII AGATCT 1 cut(s) 1776
BlnI CCTAGG 2 cut(s) 412, 815
BlpI GCTNAGC 1 cut(s) 806
BmcAI AGTACT 2 cut(s) 956, 1703
Bme1390I CCNGG 3 cut(s) 590, 605, 1255
Bme18I GGWCC 4 cut(s) 650, 660, 848, 1600
BmgT120I GGNCC 5 cut(s) 650, 660, 848, 1391, 1600
BmiI GGNNCC 1 cut(s) 1393
BmrFI CCNGG 3 cut(s) 590, 605, 1255
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 5 cut(s) 735, 982, 1554, 2081, 2269
BmtI GCTAGC 1 cut(s) 1743
BmuI ACTGGG 1 cut(s) 628
BoxI GACNNNNGTC 1 cut(s) 665
BpiI GAAGAC 2 cut(s) 1989, 2235
BpmI CTGGAG 1 cut(s) 730
Bpu10I CCTNAGC 2 cut(s) 1026, 1239
Bpu1102I GCTNAGC 1 cut(s) 806
BpuEI CTTGAG 3 cut(s) 1092, 1446, 1690
BpuMI CCSGG 2 cut(s) 590, 605
Bsa29I ATCGAT 1 cut(s) 875
BsaAI YACGTR 2 cut(s) 1516, 1861
BsaI GGTCTC 1 cut(s) 1069
BsaJI CCNNGG 6 cut(s) 228, 412, 653, 815, 1254, 2252
BsaWI WCCGGW 1 cut(s) 1867
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 468, 788, 845, 1970, 2253
Bse118I RCCGGY 1 cut(s) 838
Bse1I ACTGG 4 cut(s) 110, 623, 676, 771
Bse3DI GCAATG 1 cut(s) 1323
BseBI CCWGG 1 cut(s) 1255
BseCI ATCGAT 1 cut(s) 875
BseDI CCNNGG 6 cut(s) 228, 412, 653, 815, 1254, 2252
BseGI GGATG 6 cut(s) 194, 874, 1078, 1354, 1719, 2040
BseLI CCNNNNNNNGG 6 cut(s) 73, 468, 788, 845, 1970, 2253
BseMI GCAATG 1 cut(s) 1323
BseMII CTCAG 2 cut(s) 358, 1691
BseNI ACTGG 4 cut(s) 110, 623, 676, 771
BseRI GAGGAG 5 cut(s) 205, 208, 217, 1487, 1775
BseSI GKGCMC 1 cut(s) 558
BseXI GCAGC 7 cut(s) 129, 506, 535, 695, 1087, 1109, 1115
BsgI GTGCAG 1 cut(s) 1208
Bsh1236I CGCG 2 cut(s) 1728, 2254
BshFI GGCC 6 cut(s) 417, 635, 1392, 1771, 1796, 2140
BshVI ATCGAT 1 cut(s) 875
BsiHKAI GWGCWC 3 cut(s) 207, 558, 679
BsiSI CCGG 5 cut(s) 114, 590, 604, 839, 1868
BslFI GGGAC 3 cut(s) 620, 833, 1695
BslI CCNNNNNNNGG 6 cut(s) 73, 468, 788, 845, 1970, 2253
BsmAI GTCTC 4 cut(s) 301, 1069, 1135, 1213
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 3 cut(s) 620, 833, 1695
BsmI GAATGC 1 cut(s) 1963
BsnI GGCC 6 cut(s) 417, 635, 1392, 1771, 1796, 2140
Bso31I GGTCTC 1 cut(s) 1069
Bsp1286I GDGCHC 3 cut(s) 207, 558, 679
Bsp143I GATC 7 cut(s) 90, 872, 978, 1615, 1776, 1938, 2047
Bsp1720I GCTNAGC 1 cut(s) 806
Bsp68I TCGCGA 1 cut(s) 1728
BspANI GGCC 6 cut(s) 417, 635, 1392, 1771, 1796, 2140
BspCNI CTCAG 2 cut(s) 357, 1690
BspDI ATCGAT 1 cut(s) 875
BspFNI CGCG 2 cut(s) 1728, 2254
BspLI GGNNCC 1 cut(s) 1393
BspMAI CTGCAG 1 cut(s) 1105
BspMI ACCTGC 1 cut(s) 1051
BspOI GCTAGC 1 cut(s) 1743
BspPI GGATC 5 cut(s) 98, 880, 986, 1610, 2055
BspQI GCTCTTC 2 cut(s) 158, 358
BspTNI GGTCTC 1 cut(s) 1069
BsrBI CCGCTC 1 cut(s) 151
BsrDI GCAATG 1 cut(s) 1323
BsrFI RCCGGY 1 cut(s) 838
BsrI ACTGG 4 cut(s) 110, 623, 676, 771
BssAI RCCGGY 1 cut(s) 838
BssECI CCNNGG 6 cut(s) 228, 412, 653, 815, 1254, 2252
BssMI GATC 7 cut(s) 90, 872, 978, 1615, 1776, 1938, 2047
BssSI CACGAG 1 cut(s) 1184
BssT1I CCWWGG 2 cut(s) 412, 815
Bst2BI CACGAG 1 cut(s) 1184
Bst2UI CCWGG 1 cut(s) 1255
Bst4CI ACNGT 4 cut(s) 262, 326, 959, 1168
Bst6I CTCTTC 4 cut(s) 158, 214, 358, 477
BstAPI GCANNNNNTGC 1 cut(s) 1039
BstBAI YACGTR 2 cut(s) 1516, 1861
BstC8I GCNNGC 2 cut(s) 1741, 2096
BstDEI CTNAG 6 cut(s) 344, 806, 1026, 1239, 1677, 1773
BstDSI CCRYGG 1 cut(s) 2252
BstF5I GGATG 6 cut(s) 194, 874, 1078, 1354, 1719, 2040
BstFNI CGCG 2 cut(s) 1728, 2254
BstH2I RGCGCY 1 cut(s) 828
BstHHI GCGC 3 cut(s) 827, 836, 2056
BstKTI GATC 7 cut(s) 93, 875, 981, 1618, 1779, 1941, 2050
BstMAI GTCTC 4 cut(s) 301, 1069, 1135, 1213
BstMBI GATC 7 cut(s) 90, 872, 978, 1615, 1776, 1938, 2047
BstNI CCWGG 1 cut(s) 1255
BstNSI RCATGY 2 cut(s) 629, 1585
BstPAI GACNNNNGTC 1 cut(s) 665
BstSCI CCNGG 3 cut(s) 588, 603, 1253
BstSFI CTRYAG 3 cut(s) 1101, 1167, 1934
BstSLI GKGCMC 1 cut(s) 558
BstSNI TACGTA 1 cut(s) 1861
BstUI CGCG 2 cut(s) 1728, 2254
BstV1I GCAGC 7 cut(s) 129, 506, 535, 695, 1087, 1109, 1115
BstV2I GAAGAC 2 cut(s) 1989, 2235
BstX2I RGATCY 2 cut(s) 978, 1776
BstYI RGATCY 2 cut(s) 978, 1776
Bsu15I ATCGAT 1 cut(s) 875
BsuI GTATCC 2 cut(s) 16, 1040
BsuRI GGCC 6 cut(s) 417, 635, 1392, 1771, 1796, 2140
BsuTUI ATCGAT 1 cut(s) 875
BtgI CCRYGG 1 cut(s) 2252
BtgZI GCGATG 1 cut(s) 1709
BtsCI GGATG 6 cut(s) 194, 874, 1078, 1354, 1719, 2040
BtsI GCAGTG 1 cut(s) 1129
BtsIMutI CAGTG 4 cut(s) 533, 669, 1129, 1164
BtuMI TCGCGA 1 cut(s) 1728
BveI ACCTGC 1 cut(s) 1051
Cac8I GCNNGC 2 cut(s) 1741, 2096
CfoI GCGC 3 cut(s) 827, 836, 2056
Cfr10I RCCGGY 1 cut(s) 838
Cfr13I GGNCC 5 cut(s) 650, 660, 848, 1391, 1600
Cfr42I CCGCGG 1 cut(s) 2255
ClaI ATCGAT 1 cut(s) 875
CseI GACGC 1 cut(s) 811
Csp6I GTAC 5 cut(s) 623, 714, 955, 1702, 1862
CspCI CAANNNNNGTGG 2 cut(s) 522, 557
CviQI GTAC 5 cut(s) 623, 714, 955, 1702, 1862
DdeI CTNAG 6 cut(s) 344, 806, 1026, 1239, 1677, 1773
DpnI GATC 7 cut(s) 92, 874, 980, 1617, 1778, 1940, 2049
DpnII GATC 7 cut(s) 90, 872, 978, 1615, 1776, 1938, 2047
DrdI GACNNNNNNGTC 1 cut(s) 326
DseDI GACNNNNNNGTC 1 cut(s) 326
EaeI YGGCCR 1 cut(s) 1794
Eam1104I CTCTTC 4 cut(s) 158, 214, 358, 477
EarI CTCTTC 4 cut(s) 158, 214, 358, 477
Ecl136II GAGCTC 1 cut(s) 205
Eco105I TACGTA 1 cut(s) 1861
Eco130I CCWWGG 2 cut(s) 412, 815
Eco147I AGGCCT 3 cut(s) 417, 1771, 2140
Eco24I GRGCYC 1 cut(s) 207
Eco31I GGTCTC 1 cut(s) 1069
Eco47I GGWCC 4 cut(s) 650, 660, 848, 1600
Eco47III AGCGCT 1 cut(s) 826
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 3 cut(s) 528, 1017, 1158
EcoICRI GAGCTC 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 848
EcoRII CCWGG 1 cut(s) 1253
EcoT14I CCWWGG 2 cut(s) 412, 815
EcoT22I ATGCAT 1 cut(s) 2008
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 2 cut(s) 412, 815
Esp3I CGTCTC 1 cut(s) 301
FalI AAGNNNNNCTT 2 cut(s) 1580, 1612
FaqI GGGAC 3 cut(s) 620, 833, 1695
FauI CCCGC 1 cut(s) 1644
FauNDI CATATG 1 cut(s) 1852
FblI GTMKAC 1 cut(s) 930
FokI GGATG 6 cut(s) 181, 881, 1085, 1361, 1706, 2047
FriOI GRGCYC 1 cut(s) 207
FspBI CTAG 7 cut(s) 413, 419, 816, 1190, 1740, 1997, 2258
GlaI GCGC 3 cut(s) 826, 835, 2055
GsuI CTGGAG 1 cut(s) 730
HaeII RGCGCY 1 cut(s) 828
HaeIII GGCC 6 cut(s) 417, 635, 1392, 1771, 1796, 2140
HapII CCGG 5 cut(s) 114, 590, 604, 839, 1868
HgaI GACGC 1 cut(s) 811
HhaI GCGC 3 cut(s) 827, 836, 2056
Hin6I GCGC 3 cut(s) 825, 834, 2054
HinP1I GCGC 3 cut(s) 825, 834, 2054
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HindIII AAGCTT 4 cut(s) 1370, 1452, 1594, 2262
HinfI GANTC 7 cut(s) 63, 224, 377, 575, 596, 1244, 1250
HpaI GTTAAC 1 cut(s) 187
HpaII CCGG 5 cut(s) 114, 590, 604, 839, 1868
HphI GGTGA 2 cut(s) 152, 1489
Hpy166II GTNNAC 6 cut(s) 187, 556, 660, 931, 1127, 1912
Hpy188III TCNNGA 7 cut(s) 709, 1109, 1480, 1707, 1727, 2017, 2120
Hpy8I GTNNAC 6 cut(s) 187, 556, 660, 931, 1127, 1912
HpyAV CCTTC 8 cut(s) 81, 259, 589, 1661, 1876, 2151, 2171, 2221
HpyCH4III ACNGT 4 cut(s) 262, 326, 959, 1168
HpyCH4IV ACGT 5 cut(s) 1299, 1332, 1515, 1860, 1875
HpyF3I CTNAG 6 cut(s) 344, 806, 1026, 1239, 1677, 1773
HpySE526I ACGT 5 cut(s) 1299, 1332, 1515, 1860, 1875
HspAI GCGC 3 cut(s) 825, 834, 2054
KspAI GTTAAC 1 cut(s) 187
KspI CCGCGG 1 cut(s) 2255
Kzo9I GATC 7 cut(s) 90, 872, 978, 1615, 1776, 1938, 2047
LguI GCTCTTC 2 cut(s) 158, 358
LmnI GCTCC 3 cut(s) 232, 1030, 2180
Lsp1109I GCAGC 7 cut(s) 129, 506, 535, 695, 1087, 1109, 1115
LweI GCATC 5 cut(s) 735, 982, 1554, 2081, 2269
MaeI CTAG 7 cut(s) 413, 419, 816, 1190, 1740, 1997, 2258
MaeII ACGT 5 cut(s) 1299, 1332, 1515, 1860, 1875
MaeIII GTNAC 5 cut(s) 283, 667, 1205, 1672, 1856
MalI GATC 7 cut(s) 92, 874, 980, 1617, 1778, 1940, 2049
MbiI CCGCTC 1 cut(s) 151
MboI GATC 7 cut(s) 90, 872, 978, 1615, 1776, 1938, 2047
MflI RGATCY 2 cut(s) 978, 1776
MhlI GDGCHC 3 cut(s) 207, 558, 679
MlsI TGGCCA 1 cut(s) 1796
MluNI TGGCCA 1 cut(s) 1796
MlyI GAGTC 2 cut(s) 218, 584
MmeI TCCRAC 5 cut(s) 91, 159, 246, 1825, 2130
Mox20I TGGCCA 1 cut(s) 1796
Mph1103I ATGCAT 1 cut(s) 2008
MscI TGGCCA 1 cut(s) 1796
MseI TTAA 4 cut(s) 186, 1151, 1878, 1988
Msp20I TGGCCA 1 cut(s) 1796
MspA1I CMGCKG 3 cut(s) 1100, 1280, 2254
MspI CCGG 5 cut(s) 114, 590, 604, 839, 1868
MspR9I CCNGG 3 cut(s) 590, 605, 1255
Mva1269I GAATGC 1 cut(s) 1963
MvaI CCWGG 1 cut(s) 1255
MvnI CGCG 2 cut(s) 1728, 2254
NciI CCSGG 2 cut(s) 590, 605
NdeI CATATG 1 cut(s) 1852
NdeII GATC 7 cut(s) 90, 872, 978, 1615, 1776, 1938, 2047
NheI GCTAGC 1 cut(s) 1739
NlaIV GGNNCC 1 cut(s) 1393
NmuCI GTSAC 2 cut(s) 283, 667
NruI TCGCGA 1 cut(s) 1728
NsiI ATGCAT 1 cut(s) 2008
NspI RCATGY 2 cut(s) 629, 1585
PceI AGGCCT 3 cut(s) 417, 1771, 2140
PciI ACATGT 1 cut(s) 625
PciSI GCTCTTC 2 cut(s) 158, 358
PctI GAATGC 1 cut(s) 1963
PfeI GAWTC 5 cut(s) 63, 377, 596, 1244, 1250
PflFI GACNNNGTC 1 cut(s) 926
PfoI TCCNGGA 1 cut(s) 603
PleI GAGTC 2 cut(s) 218, 583
PpsI GAGTC 2 cut(s) 218, 583
Ppu21I YACGTR 2 cut(s) 1516, 1861
PpuMI RGGWCCY 1 cut(s) 848
PscI ACATGT 1 cut(s) 625
PshAI GACNNNNGTC 1 cut(s) 665
PsiI TTATAA 1 cut(s) 1401
Psp124BI GAGCTC 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 1299
Psp5II RGGWCCY 1 cut(s) 848
Psp6I CCWGG 1 cut(s) 1253
PspGI CCWGG 1 cut(s) 1253
PspN4I GGNNCC 1 cut(s) 1393
PspPI GGNCC 5 cut(s) 650, 660, 848, 1391, 1600
PspPPI RGGWCCY 1 cut(s) 848
PstI CTGCAG 1 cut(s) 1105
PsuI RGATCY 2 cut(s) 978, 1776
PsyI GACNNNGTC 1 cut(s) 926
PvuII CAGCTG 2 cut(s) 1100, 1280
RruI TCGCGA 1 cut(s) 1728
RsaI GTAC 5 cut(s) 624, 715, 956, 1703, 1863
RsaNI GTAC 5 cut(s) 623, 714, 955, 1702, 1862
SacI GAGCTC 1 cut(s) 207
SacII CCGCGG 1 cut(s) 2255
SapI GCTCTTC 2 cut(s) 158, 358
SaqAI TTAA 4 cut(s) 186, 1151, 1878, 1988
Sau3AI GATC 7 cut(s) 90, 872, 978, 1615, 1776, 1938, 2047
Sau96I GGNCC 5 cut(s) 650, 660, 848, 1391, 1600
ScaI AGTACT 2 cut(s) 956, 1703
SchI GAGTC 2 cut(s) 218, 584
ScrFI CCNGG 3 cut(s) 590, 605, 1255
SduI GDGCHC 3 cut(s) 207, 558, 679
SfaNI GCATC 5 cut(s) 735, 982, 1554, 2081, 2269
SfcI CTRYAG 3 cut(s) 1101, 1167, 1934
Sfr303I CCGCGG 1 cut(s) 2255
SgrBI CCGCGG 1 cut(s) 2255
SinI GGWCC 4 cut(s) 650, 660, 848, 1600
SmlI CTYRAG 3 cut(s) 1107, 1461, 1705
SmoI CTYRAG 3 cut(s) 1107, 1461, 1705
SnaBI TACGTA 1 cut(s) 1861
SseBI AGGCCT 3 cut(s) 417, 1771, 2140
SspI AATATT 1 cut(s) 1317
SspMI CTAG 7 cut(s) 413, 419, 816, 1190, 1740, 1997, 2258
SstI GAGCTC 1 cut(s) 207
StuI AGGCCT 3 cut(s) 417, 1771, 2140
StyD4I CCNGG 3 cut(s) 588, 603, 1253
StyI CCWWGG 2 cut(s) 412, 815
TaaI ACNGT 4 cut(s) 262, 326, 959, 1168
TaiI ACGT 5 cut(s) 1302, 1335, 1518, 1863, 1878
TatI WGTACW 2 cut(s) 954, 1701
TauI GCSGC 4 cut(s) 515, 1343, 2254, 2257
TfiI GAWTC 5 cut(s) 63, 377, 596, 1244, 1250
Tru1I TTAA 4 cut(s) 186, 1151, 1878, 1988
Tru9I TTAA 4 cut(s) 186, 1151, 1878, 1988
TscAI CASTG 4 cut(s) 540, 676, 1129, 1171
TseFI GTSAC 2 cut(s) 283, 667
TseI GCWGC 7 cut(s) 117, 494, 548, 683, 1097, 1100, 1103
Tsp45I GTSAC 2 cut(s) 283, 667
TspDTI ATGAA 5 cut(s) 361, 1042, 1647, 1995, 2190
TspGWI ACGGA 5 cut(s) 663, 731, 878, 1153, 1361
TspRI CASTG 4 cut(s) 540, 676, 1129, 1171
Tth111I GACNNNGTC 1 cut(s) 926
VneI GTGCAC 1 cut(s) 554
VpaK11BI GGWCC 4 cut(s) 650, 660, 848, 1600
XapI RAATTY 8 cut(s) 11, 122, 364, 1036, 1286, 1800, 2154, 2202
XceI RCATGY 2 cut(s) 629, 1585
XcmI CCANNNNNNNNNTGG 2 cut(s) 705, 769
XmaJI CCTAGG 2 cut(s) 412, 815
XmiI GTMKAC 1 cut(s) 930
XspI CTAG 7 cut(s) 413, 419, 816, 1190, 1740, 1997, 2258
ZrmI AGTACT 2 cut(s) 956, 1703
Zsp2I ATGCAT 1 cut(s) 2008
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.