pycom15g14060

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000775
Physical Location & Seq
Forward (+)
9590715 .. 9593934
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g14060.3

Sequence Viewer

Length: 2304 bp
ATGGTCTCCAAATTTTCAGTTCTACCATTCTTCTTCTTTTTCCATATTTTGTTTGCTTTTAGATTCCAACCTTCGGAGGCACAATCTTGGATCAAAGCTGCATACTGGTATTCCGGCAGCGAATTCCCTATTTCCGACATCAACTCTCCGCTCTTCACTCACCTTATTTACGCTTACGCAGATGTTAACTCATCATCCTATGAGCTCTCTTCCTTCTCCTCCGACCCCTCGGAGCAGTACTACTCCACCTTCACGCCAACTGTCAAGCTAAAGAACCCATCAGTCACTACTCTCGTCTCCATTGGCGGTAACGCAGACTACGGGGTCATTTCAACCATGGTCAGTAGCTCTTCTCACAGAAAGTCTTTCATTGATTCTTCGATAATTTTCGCAAGGCGTTATGGCTTCCTAGGCCTAGACTTTTGTTGGGTTTCAGCTAACACAAGCTCGGACATGACCAATATGGGGAAGTTATTTGAAGAGTGGCGGGCAGCTATCGCTTCTGAAGCCACAAAAAACAGCCAAACACCACTGATTTTGACTGCTGCCGTGCAGCACAAGCCAGATTTTGAGTCCTTCGCTTTCCCGGTTGAATCAATCCGGGACAACTTGAACTGGGTACATGTCATTGCCTATGACTATTACGTACCTCAGCGGATCAATTTCACCGGTGCTCATGCAGCACTATTCGATCCATCGAGTGACCTTAACACGGACTATGGTATCAAATCATGGATTGGCAGGGGATTATCTGCTTCCAAACTGGTTTTGGGATTGCCTTTCTATGGATATGCTTGGACGCTAAGCAATCCCAGGGACAGCGCTATTGGTGCACCGGCTAAAGGTCCTGCTATTACCAAGGTTGGAGCGATGAGGTACAAGGATATCAAAGGCTACACTGATAGATATGGGGCTGACATAGTCTACAATGCTACTTATGTAGCGAAGTACTGTGTGATCGGTTCAGCTTGGATCGGTTTCGATGATGCTGAAGTTGTGAAAATAAAAGTTTCTTATGCTAAGGAGCAGAAATTGCTTGGATACTTCATCTGGCAGGTCTCATATGATGATAATTGGGTGCTTTCTCTTGCAGCTGCAGCTCAAGAGGGTGGAGGCAGGGGACAAAACAAACGGAGACTTCTTGTAATCGTTCTGACCACTATAGCTTCAGTTATTCTCGTGCTAGGCTCCGCGTTGAGTTACTTCCGCATGAGACTGCACAAATCAAAATCTAAGGAATCAGAATCCAGGGCAAATGATATAGCAGACACAGCTGGAAAATTCAACAGCAACGTTCCTAATCTGAAAATATTTAGCATTGCTGACGTTGGGGTGGCAACGGGTGGATTTTCGATTGAAAATAAGCTTGGAGAGGGCGGTTATGGCCCCGTTTATAAGGGAGTATTGCCAGATGGACAAGAAATAGCTGTGAAGAAACTATCAAAAGCTTCAACTCAAGGATTTGAGGAGTTCAAGAACGAGGTCATGCTCACCTCAAAACTACAACACGTAAATCTTGTTAGGGTTTTGGGATTTTGCATCGAACACCATGAGCAAATGCTGATTTACGAGTACATGCCAAAGAAAAGCTTGGACCTCTACCTCTTTGGTCCTGTTAGAAGATATGAATTGGATTGGAGAAAGCGGGTTCAAATTATCGAAGGGGTTACTCAGGGACTTCTTTACCTCCAAGAGTACTCAAGATTGACAATCATCCATCGCGATCTGAAAGCTAGCAACATTTTACTTGATGAGGAGATGAGGCCTAAGATCTCGGATTTTGGTATGGCCAGAATTTTTTCAAGGGCTGAACTCGAAGCAAATACAGGTCGAATTGTTGGAACATATGGTTACATACCACCGGAATACGTTAAGAAAGGTTTATACTCCACTAAATCAGATGTTTACAGCTTCGGAGTTCTCCTTCTACAGATCATAAGTGGCAAGAAGAATGCCTCCTACTATGGTTCAGATGAAGACTTAAACCTACTAGAATATGCATATTTACTTCGGAAACAAGGCAAAGGGATGGAGTTTATGGATCCTACACTTGACGATACACATTCTTTATGCAAATTGATGAGATGCTTGCAAATTGCTCTCCTATGTGTTCAAGAAAATGCAAACGATAGGCCTTCGATGTTGGAAATTTCTTCAATGCTACAAAGTGAAGGAGCTAAAATGGAAAATCCCAAAATTCCAGCCTTCTCAAAAAGAAACGAAGACGAAGAAACTAATCCCACACCGCAGCCAGAAGCCTGTTCCATCGATGATGCAACAATCTCAGAAGTTGTAGGCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 ko01100 map00520 map01100
Pfam Domains
Protein Families
Glyco_hydro_18 Pkinase Pkinase_Tyr

Protein Analysis

768

Amino Acids

85.84

Weight (kDa)

6.67

Isoelectric Point (pI)

35.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1395
AasI GACNNNNNNGTC 1 cut(s) 323
Acc36I ACCTGC 1 cut(s) 1045
AccBSI CCGCTC 1 cut(s) 151
AccI GTMKAC 1 cut(s) 924
AccII CGCG 2 cut(s) 1193, 1722
AciI CCGC 9 cut(s) 149, 306, 487, 655, 1191, 1207, 1377, 1645, 2246
AclI AACGTT 1 cut(s) 1293
AclWI GGATC 6 cut(s) 98, 665, 686, 980, 2036, 2049
AcoI YGGCCR 1 cut(s) 1788
AcsI RAATTY 6 cut(s) 11, 122, 1280, 1794, 2148, 2196
AcuI CTGAAG 3 cut(s) 525, 1011, 1152
AfaI GTAC 7 cut(s) 239, 621, 648, 878, 950, 1574, 1697
AfeI AGCGCT 1 cut(s) 823
AfiI CCNNNNNNNGG 5 cut(s) 73, 465, 712, 785, 842
AflIII ACRYGT 2 cut(s) 622, 1507
AgeI ACCGGT 1 cut(s) 668
AjnI CCWGG 2 cut(s) 812, 1247
AjuI GAANNNNNNNTTGG 2 cut(s) 1350, 1382
Alw21I GWGCWC 3 cut(s) 207, 676, 835
Alw26I GTCTC 5 cut(s) 10, 301, 1063, 1129, 1207
Alw44I GTGCAC 1 cut(s) 831
AlwI GGATC 6 cut(s) 98, 665, 686, 980, 2036, 2049
Aor51HI AGCGCT 1 cut(s) 823
AoxI GGCC 6 cut(s) 412, 1384, 1763, 1788, 2132, 2296
ApaLI GTGCAC 1 cut(s) 831
ApoI RAATTY 6 cut(s) 11, 122, 1280, 1794, 2148, 2196
AsiGI ACCGGT 1 cut(s) 668
Asp700I GAANNNNTTC 1 cut(s) 1798
AspA2I CCTAGG 1 cut(s) 409
AspLEI GCGC 1 cut(s) 824
AspS9I GGNCC 4 cut(s) 845, 1385, 1594, 1610
AsuC2I CCSGG 2 cut(s) 587, 602
AsuHPI GGTGA 3 cut(s) 152, 658, 1483
AsuNHI GCTAGC 1 cut(s) 1733
AvaII GGWCC 3 cut(s) 845, 1594, 1610
AvrII CCTAGG 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 835
BaeI ACNNNNGTAYC 2 cut(s) 706, 739
BalI TGGCCA 1 cut(s) 1790
BamHI GGATCC 1 cut(s) 2041
BanII GRGCYC 1 cut(s) 207
BauI CACGAG 1 cut(s) 1178
BbsI GAAGAC 2 cut(s) 1983, 2229
Bbv12I GWGCWC 3 cut(s) 207, 676, 835
BbvCI CCTCAGC 1 cut(s) 651
BccI CCATC 6 cut(s) 286, 703, 1406, 1725, 2023, 2273
BceAI ACGGC 1 cut(s) 533
BciT130I CCWGG 2 cut(s) 814, 1249
BciVI GTATCC 1 cut(s) 1034
BcnI CCSGG 2 cut(s) 587, 602
BcoDI GTCTC 5 cut(s) 10, 301, 1063, 1129, 1207
BfaI CTAG 5 cut(s) 410, 416, 1184, 1734, 1991
BfmI CTRYAG 3 cut(s) 1095, 1161, 1928
BfoI RGCGCY 1 cut(s) 825
BfuAI ACCTGC 1 cut(s) 1045
BfuI GTATCC 1 cut(s) 1034
BglII AGATCT 1 cut(s) 1770
BlnI CCTAGG 1 cut(s) 409
BlpI GCTNAGC 1 cut(s) 803
BmcAI AGTACT 3 cut(s) 239, 950, 1697
Bme1390I CCNGG 4 cut(s) 587, 602, 814, 1249
Bme18I GGWCC 3 cut(s) 845, 1594, 1610
BmgT120I GGNCC 4 cut(s) 845, 1385, 1594, 1610
BmiI GGNNCC 3 cut(s) 1189, 1387, 2043
BmrFI CCNGG 4 cut(s) 587, 602, 814, 1249
BmrI ACTGGG 1 cut(s) 625
BmsI GCATC 4 cut(s) 976, 1548, 2075, 2263
BmtI GCTAGC 1 cut(s) 1737
BmuI ACTGGG 1 cut(s) 625
BpiI GAAGAC 2 cut(s) 1983, 2229
Bpu10I CCTNAGC 2 cut(s) 651, 1020
Bpu1102I GCTNAGC 1 cut(s) 803
BpuEI CTTGAG 3 cut(s) 1086, 1440, 1684
BpuMI CCSGG 2 cut(s) 587, 602
Bsa29I ATCGAT 1 cut(s) 2268
BsaAI YACGTR 2 cut(s) 646, 1510
BsaI GGTCTC 2 cut(s) 10, 1063
BsaJI CCNNGG 7 cut(s) 228, 336, 409, 812, 813, 858, 1248
BsaWI WCCGGW 2 cut(s) 668, 1861
Bsc4I CCNNNNNNNGG 5 cut(s) 73, 465, 712, 785, 842
Bse118I RCCGGY 2 cut(s) 668, 835
Bse1I ACTGG 3 cut(s) 110, 620, 768
Bse3DI GCAATG 2 cut(s) 627, 1317
BseBI CCWGG 2 cut(s) 814, 1249
BseCI ATCGAT 1 cut(s) 2268
BseDI CCNNGG 7 cut(s) 228, 336, 409, 812, 813, 858, 1248
BseGI GGATG 3 cut(s) 194, 1713, 2034
BseLI CCNNNNNNNGG 5 cut(s) 73, 465, 712, 785, 842
BseMI GCAATG 2 cut(s) 627, 1317
BseMII CTCAG 3 cut(s) 665, 1685, 2298
BseNI ACTGG 3 cut(s) 110, 620, 768
BseRI GAGGAG 3 cut(s) 208, 1481, 1769
BseSI GKGCMC 1 cut(s) 835
BsgI GTGCAG 2 cut(s) 572, 1202
Bsh1236I CGCG 2 cut(s) 1193, 1722
BshFI GGCC 6 cut(s) 414, 1386, 1765, 1790, 2134, 2298
BshTI ACCGGT 1 cut(s) 668
BshVI ATCGAT 1 cut(s) 2268
BsiHKAI GWGCWC 3 cut(s) 207, 676, 835
BsiSI CCGG 6 cut(s) 114, 587, 601, 669, 836, 1862
BslFI GGGAC 4 cut(s) 617, 830, 1134, 1689
BslI CCNNNNNNNGG 5 cut(s) 73, 465, 712, 785, 842
BsmAI GTCTC 5 cut(s) 10, 301, 1063, 1129, 1207
BsmBI CGTCTC 1 cut(s) 301
BsmFI GGGAC 4 cut(s) 617, 830, 1134, 1689
BsmI GAATGC 1 cut(s) 1957
BsnI GGCC 6 cut(s) 414, 1386, 1765, 1790, 2134, 2298
Bso31I GGTCTC 2 cut(s) 10, 1063
Bsp1286I GDGCHC 3 cut(s) 207, 676, 835
Bsp143I GATC 9 cut(s) 90, 657, 691, 957, 972, 1723, 1770, 1932, 2041
Bsp1720I GCTNAGC 1 cut(s) 803
Bsp19I CCATGG 1 cut(s) 336
Bsp68I TCGCGA 1 cut(s) 1722
BspACI CCGC 9 cut(s) 149, 306, 487, 655, 1191, 1207, 1377, 1645, 2246
BspANI GGCC 6 cut(s) 414, 1386, 1765, 1790, 2134, 2298
BspCNI CTCAG 3 cut(s) 664, 1684, 2297
BspDI ATCGAT 1 cut(s) 2268
BspFNI CGCG 2 cut(s) 1193, 1722
BspLI GGNNCC 3 cut(s) 1189, 1387, 2043
BspMAI CTGCAG 1 cut(s) 1099
BspMI ACCTGC 1 cut(s) 1045
BspOI GCTAGC 1 cut(s) 1737
BspPI GGATC 6 cut(s) 98, 665, 686, 980, 2036, 2049
BspQI GCTCTTC 2 cut(s) 158, 355
BspTNI GGTCTC 2 cut(s) 10, 1063
BsrBI CCGCTC 1 cut(s) 151
BsrDI GCAATG 2 cut(s) 627, 1317
BsrFI RCCGGY 2 cut(s) 668, 835
BsrI ACTGG 3 cut(s) 110, 620, 768
BssAI RCCGGY 2 cut(s) 668, 835
BssECI CCNNGG 7 cut(s) 228, 336, 409, 812, 813, 858, 1248
BssMI GATC 9 cut(s) 90, 657, 691, 957, 972, 1723, 1770, 1932, 2041
BssSI CACGAG 1 cut(s) 1178
BssT1I CCWWGG 3 cut(s) 336, 409, 858
Bst2BI CACGAG 1 cut(s) 1178
Bst2UI CCWGG 2 cut(s) 814, 1249
Bst4CI ACNGT 2 cut(s) 262, 953
Bst6I CTCTTC 4 cut(s) 158, 214, 355, 474
BstAPI GCANNNNNTGC 1 cut(s) 1033
BstBAI YACGTR 2 cut(s) 646, 1510
BstC8I GCNNGC 3 cut(s) 489, 1735, 2090
BstDEI CTNAG 7 cut(s) 651, 803, 1020, 1233, 1671, 1767, 2284
BstDSI CCRYGG 1 cut(s) 336
BstF5I GGATG 3 cut(s) 194, 1713, 2034
BstFNI CGCG 2 cut(s) 1193, 1722
BstH2I RGCGCY 1 cut(s) 825
BstHHI GCGC 1 cut(s) 824
BstKTI GATC 9 cut(s) 93, 660, 694, 960, 975, 1726, 1773, 1935, 2044
BstMAI GTCTC 5 cut(s) 10, 301, 1063, 1129, 1207
BstMBI GATC 9 cut(s) 90, 657, 691, 957, 972, 1723, 1770, 1932, 2041
BstNI CCWGG 2 cut(s) 814, 1249
BstNSI RCATGY 2 cut(s) 626, 1579
BstSCI CCNGG 4 cut(s) 585, 600, 812, 1247
BstSFI CTRYAG 3 cut(s) 1095, 1161, 1928
BstSLI GKGCMC 1 cut(s) 835
BstSNI TACGTA 1 cut(s) 646
BstUI CGCG 2 cut(s) 1193, 1722
BstV2I GAAGAC 2 cut(s) 1983, 2229
BstX2I RGATCY 2 cut(s) 1770, 2041
BstYI RGATCY 2 cut(s) 1770, 2041
Bsu15I ATCGAT 1 cut(s) 2268
BsuI GTATCC 1 cut(s) 1034
BsuRI GGCC 6 cut(s) 414, 1386, 1765, 1790, 2134, 2298
BsuTUI ATCGAT 1 cut(s) 2268
BtgI CCRYGG 1 cut(s) 336
BtgZI GCGATG 2 cut(s) 884, 1703
BtsCI GGATG 3 cut(s) 194, 1713, 2034
BtsIMutI CAGTG 2 cut(s) 530, 897
BtuMI TCGCGA 1 cut(s) 1722
BveI ACCTGC 1 cut(s) 1045
Cac8I GCNNGC 3 cut(s) 489, 1735, 2090
CfoI GCGC 1 cut(s) 824
Cfr10I RCCGGY 2 cut(s) 668, 835
Cfr13I GGNCC 4 cut(s) 845, 1385, 1594, 1610
ClaI ATCGAT 1 cut(s) 2268
CseI GACGC 1 cut(s) 808
Csp6I GTAC 7 cut(s) 238, 620, 647, 877, 949, 1573, 1696
CspAI ACCGGT 1 cut(s) 668
CspCI CAANNNNNGTGG 2 cut(s) 519, 554
CviAII CATG 9 cut(s) 337, 454, 623, 677, 732, 1210, 1486, 1550, 1576
CviQI GTAC 7 cut(s) 238, 620, 647, 877, 949, 1573, 1696
DdeI CTNAG 7 cut(s) 651, 803, 1020, 1233, 1671, 1767, 2284
DpnI GATC 9 cut(s) 92, 659, 693, 959, 974, 1725, 1772, 1934, 2043
DpnII GATC 9 cut(s) 90, 657, 691, 957, 972, 1723, 1770, 1932, 2041
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
EaeI YGGCCR 1 cut(s) 1788
Eam1104I CTCTTC 4 cut(s) 158, 214, 355, 474
EarI CTCTTC 4 cut(s) 158, 214, 355, 474
Ecl136II GAGCTC 1 cut(s) 205
Eco105I TACGTA 1 cut(s) 646
Eco130I CCWWGG 3 cut(s) 336, 409, 858
Eco147I AGGCCT 3 cut(s) 414, 1765, 2134
Eco24I GRGCYC 1 cut(s) 207
Eco31I GGTCTC 2 cut(s) 10, 1063
Eco32I GATATC 1 cut(s) 886
Eco47I GGWCC 3 cut(s) 845, 1594, 1610
Eco47III AGCGCT 1 cut(s) 823
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 3 cut(s) 525, 1011, 1152
EcoICRI GAGCTC 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 845
EcoRI GAATTC 1 cut(s) 122
EcoRII CCWGG 2 cut(s) 812, 1247
EcoRV GATATC 1 cut(s) 886
EcoT14I CCWWGG 3 cut(s) 336, 409, 858
EcoT22I ATGCAT 1 cut(s) 2002
EcoT38I GRGCYC 1 cut(s) 207
ErhI CCWWGG 3 cut(s) 336, 409, 858
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 9 cut(s) 340, 457, 626, 680, 735, 1213, 1489, 1553, 1579
FalI AAGNNNNNCTT 2 cut(s) 1574, 1606
FaqI GGGAC 4 cut(s) 617, 830, 1134, 1689
FatI CATG 9 cut(s) 336, 453, 622, 676, 731, 1209, 1485, 1549, 1575
FauI CCCGC 2 cut(s) 480, 1638
FauNDI CATATG 2 cut(s) 1063, 1846
FblI GTMKAC 1 cut(s) 924
FokI GGATG 3 cut(s) 181, 1700, 2041
FriOI GRGCYC 1 cut(s) 207
FspBI CTAG 5 cut(s) 410, 416, 1184, 1734, 1991
GlaI GCGC 1 cut(s) 823
HaeII RGCGCY 1 cut(s) 825
HaeIII GGCC 6 cut(s) 414, 1386, 1765, 1790, 2134, 2298
HapII CCGG 6 cut(s) 114, 587, 601, 669, 836, 1862
HgaI GACGC 1 cut(s) 808
HhaI GCGC 1 cut(s) 824
Hin1II CATG 9 cut(s) 340, 457, 626, 680, 735, 1213, 1489, 1553, 1579
Hin6I GCGC 1 cut(s) 822
HinP1I GCGC 1 cut(s) 822
HincII GTYRAC 1 cut(s) 187
HindII GTYRAC 1 cut(s) 187
HindIII AAGCTT 3 cut(s) 1364, 1446, 1588
HinfI GANTC 6 cut(s) 63, 374, 572, 593, 1238, 1244
HpaI GTTAAC 1 cut(s) 187
HpaII CCGG 6 cut(s) 114, 587, 601, 669, 836, 1862
HphI GGTGA 3 cut(s) 152, 658, 1483
Hpy166II GTNNAC 4 cut(s) 187, 833, 925, 1906
Hpy188III TCNNGA 5 cut(s) 1103, 1474, 1701, 1721, 2114
Hpy8I GTNNAC 4 cut(s) 187, 833, 925, 1906
HpyAV CCTTC 9 cut(s) 81, 223, 259, 586, 1655, 1934, 2145, 2165, 2215
HpyCH4III ACNGT 2 cut(s) 262, 953
HpyCH4IV ACGT 5 cut(s) 645, 1293, 1326, 1509, 1869
HpyF3I CTNAG 7 cut(s) 651, 803, 1020, 1233, 1671, 1767, 2284
HpySE526I ACGT 5 cut(s) 645, 1293, 1326, 1509, 1869
Hsp92II CATG 9 cut(s) 340, 457, 626, 680, 735, 1213, 1489, 1553, 1579
HspAI GCGC 1 cut(s) 822
KspAI GTTAAC 1 cut(s) 187
Kzo9I GATC 9 cut(s) 90, 657, 691, 957, 972, 1723, 1770, 1932, 2041
LguI GCTCTTC 2 cut(s) 158, 355
LmnI GCTCC 5 cut(s) 232, 866, 1024, 1193, 2174
LweI GCATC 4 cut(s) 976, 1548, 2075, 2263
MaeI CTAG 5 cut(s) 410, 416, 1184, 1734, 1991
MaeII ACGT 5 cut(s) 645, 1293, 1326, 1509, 1869
MaeIII GTNAC 6 cut(s) 283, 308, 701, 1199, 1666, 1850
MalI GATC 9 cut(s) 92, 659, 693, 959, 974, 1725, 1772, 1934, 2043
MbiI CCGCTC 1 cut(s) 151
MboI GATC 9 cut(s) 90, 657, 691, 957, 972, 1723, 1770, 1932, 2041
MflI RGATCY 2 cut(s) 1770, 2041
MhlI GDGCHC 3 cut(s) 207, 676, 835
MlsI TGGCCA 1 cut(s) 1790
MluNI TGGCCA 1 cut(s) 1790
MlyI GAGTC 1 cut(s) 581
MmeI TCCRAC 6 cut(s) 91, 159, 246, 844, 1819, 2124
Mox20I TGGCCA 1 cut(s) 1790
Mph1103I ATGCAT 1 cut(s) 2002
MroXI GAANNNNTTC 1 cut(s) 1798
MscI TGGCCA 1 cut(s) 1790
MseI TTAA 4 cut(s) 186, 708, 1872, 1982
Msp20I TGGCCA 1 cut(s) 1790
MspA1I CMGCKG 3 cut(s) 655, 1094, 1274
MspI CCGG 6 cut(s) 114, 587, 601, 669, 836, 1862
MspR9I CCNGG 4 cut(s) 587, 602, 814, 1249
Mva1269I GAATGC 1 cut(s) 1957
MvaI CCWGG 2 cut(s) 814, 1249
MvnI CGCG 2 cut(s) 1193, 1722
NciI CCSGG 2 cut(s) 587, 602
NcoI CCATGG 1 cut(s) 336
NdeI CATATG 2 cut(s) 1063, 1846
NdeII GATC 9 cut(s) 90, 657, 691, 957, 972, 1723, 1770, 1932, 2041
NheI GCTAGC 1 cut(s) 1733
NlaIII CATG 9 cut(s) 340, 457, 626, 680, 735, 1213, 1489, 1553, 1579
NlaIV GGNNCC 3 cut(s) 1189, 1387, 2043
NmuCI GTSAC 2 cut(s) 283, 701
NruI TCGCGA 1 cut(s) 1722
NsiI ATGCAT 1 cut(s) 2002
NspI RCATGY 2 cut(s) 626, 1579
PasI CCCWGGG 1 cut(s) 813
PceI AGGCCT 3 cut(s) 414, 1765, 2134
PciI ACATGT 1 cut(s) 622
PciSI GCTCTTC 2 cut(s) 158, 355
PctI GAATGC 1 cut(s) 1957
PdmI GAANNNNTTC 1 cut(s) 1798
PfeI GAWTC 5 cut(s) 63, 374, 593, 1238, 1244
PflFI GACNNNGTC 1 cut(s) 920
PfoI TCCNGGA 1 cut(s) 600
PinAI ACCGGT 1 cut(s) 668
PleI GAGTC 1 cut(s) 580
PpsI GAGTC 1 cut(s) 580
Ppu21I YACGTR 2 cut(s) 646, 1510
PpuMI RGGWCCY 1 cut(s) 845
PscI ACATGT 1 cut(s) 622
PsiI TTATAA 1 cut(s) 1395
Psp124BI GAGCTC 1 cut(s) 207
Psp1406I AACGTT 1 cut(s) 1293
Psp5II RGGWCCY 1 cut(s) 845
Psp6I CCWGG 2 cut(s) 812, 1247
PspGI CCWGG 2 cut(s) 812, 1247
PspN4I GGNNCC 3 cut(s) 1189, 1387, 2043
PspPI GGNCC 4 cut(s) 845, 1385, 1594, 1610
PspPPI RGGWCCY 1 cut(s) 845
PstI CTGCAG 1 cut(s) 1099
PsuI RGATCY 2 cut(s) 1770, 2041
PsyI GACNNNGTC 1 cut(s) 920
PvuII CAGCTG 2 cut(s) 1094, 1274
RruI TCGCGA 1 cut(s) 1722
RsaI GTAC 7 cut(s) 239, 621, 648, 878, 950, 1574, 1697
RsaNI GTAC 7 cut(s) 238, 620, 647, 877, 949, 1573, 1696
SacI GAGCTC 1 cut(s) 207
SapI GCTCTTC 2 cut(s) 158, 355
SaqAI TTAA 4 cut(s) 186, 708, 1872, 1982
Sau3AI GATC 9 cut(s) 90, 657, 691, 957, 972, 1723, 1770, 1932, 2041
Sau96I GGNCC 4 cut(s) 845, 1385, 1594, 1610
ScaI AGTACT 3 cut(s) 239, 950, 1697
SchI GAGTC 1 cut(s) 581
ScrFI CCNGG 4 cut(s) 587, 602, 814, 1249
SduI GDGCHC 3 cut(s) 207, 676, 835
SfaNI GCATC 4 cut(s) 976, 1548, 2075, 2263
SfcI CTRYAG 3 cut(s) 1095, 1161, 1928
SgrAI CRCCGGYG 1 cut(s) 668
SinI GGWCC 3 cut(s) 845, 1594, 1610
SmlI CTYRAG 3 cut(s) 1101, 1455, 1699
SmoI CTYRAG 3 cut(s) 1101, 1455, 1699
SnaBI TACGTA 1 cut(s) 646
SseBI AGGCCT 3 cut(s) 414, 1765, 2134
SsiI CCGC 9 cut(s) 149, 306, 487, 655, 1191, 1207, 1377, 1645, 2246
SspI AATATT 1 cut(s) 1311
SspMI CTAG 5 cut(s) 410, 416, 1184, 1734, 1991
SstI GAGCTC 1 cut(s) 207
StuI AGGCCT 3 cut(s) 414, 1765, 2134
StyD4I CCNGG 4 cut(s) 585, 600, 812, 1247
StyI CCWWGG 3 cut(s) 336, 409, 858
TaaI ACNGT 2 cut(s) 262, 953
TaiI ACGT 5 cut(s) 648, 1296, 1329, 1512, 1872
TatI WGTACW 4 cut(s) 237, 948, 1572, 1695
TfiI GAWTC 5 cut(s) 63, 374, 593, 1238, 1244
Tru1I TTAA 4 cut(s) 186, 708, 1872, 1982
Tru9I TTAA 4 cut(s) 186, 708, 1872, 1982
TscAI CASTG 2 cut(s) 537, 904
TseFI GTSAC 2 cut(s) 283, 701
Tsp45I GTSAC 2 cut(s) 283, 701
TspDTI ATGAA 4 cut(s) 358, 1036, 1641, 1989
TspGWI ACGGA 2 cut(s) 728, 1147
TspRI CASTG 2 cut(s) 537, 904
Tth111I GACNNNGTC 1 cut(s) 920
VneI GTGCAC 1 cut(s) 831
VpaK11BI GGWCC 3 cut(s) 845, 1594, 1610
XapI RAATTY 6 cut(s) 11, 122, 1280, 1794, 2148, 2196
XceI RCATGY 2 cut(s) 626, 1579
XcmI CCANNNNNNNNNTGG 1 cut(s) 766
XmaJI CCTAGG 1 cut(s) 409
XmiI GTMKAC 1 cut(s) 924
XmnI GAANNNNTTC 1 cut(s) 1798
XspI CTAG 5 cut(s) 410, 416, 1184, 1734, 1991
ZrmI AGTACT 3 cut(s) 239, 950, 1697
Zsp2I ATGCAT 1 cut(s) 2002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.